FastQCFastQC Report
Sun 15 May 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.Partitivirus_1_segment_2.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.Partitivirus_1_segment_2.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length15-35
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTGCAGCCGTCGTCATCACTGAGAATTCGAATC25.88235294117647No Hit
TTCTTTGTCCTCTCACGGCT25.88235294117647No Hit
TTCCAACACTGAGTCTGGTCTGA25.88235294117647No Hit
TTTGCTGACGACCTGGGCTTTGA25.88235294117647No Hit
TGAACGTGTTGGTCCTGCTGAGT25.88235294117647No Hit
AATGCTCTCGGAAGTATGTCTCGTGTTACTGG12.941176470588235No Hit
CACGCGACATCCATGGCGAAG12.941176470588235No Hit
CTCGGAAGTATGTCTCGTGTTAC12.941176470588235No Hit
TCCAAATGCTCTCGGAAGTATGTCTCGTGTTAC12.941176470588235No Hit
CGACACCCGCCGCCC12.941176470588235No Hit
TACTATGAACGTGTTGGTC12.941176470588235No Hit
CCCATGTGTTCTACT12.941176470588235No Hit
TTGCTGACGACATGGGCTTTGA12.941176470588235No Hit
AAATGCTCTCGGAAGTATGTCTCGTGTTAC12.941176470588235No Hit
TCACTGAGGATTCGAATCAGATCGACCCAGCCCGT12.941176470588235No Hit
TCAGCACTGAACGCTTCTGGCACTCCAAATGCT12.941176470588235No Hit
TCGGAAGTATGTCTCGTGTT12.941176470588235No Hit
TCTGATCGACACCCGAATGGCAA12.941176470588235No Hit
TCAGCACTGAACGCTTCTGGCACTCCA12.941176470588235No Hit
TCTTTCTGTGGTCTCGTTTCTTCT12.941176470588235No Hit
TTTCTCGACCCGCCGTGTCTGGC12.941176470588235No Hit
CGTGCAGCCGTCGTCATC12.941176470588235No Hit
TGCTGACGACCTGGGCTTTGATA12.941176470588235No Hit
GAACGTATCAAGCTCCATGGGGA12.941176470588235No Hit
TCTCACGGCTATTCCAACACTG12.941176470588235No Hit
TTTAGCTACCCGTACGTCGTACA12.941176470588235No Hit
TTCCCCGACCCGCGCC12.941176470588235No Hit
ACCCAGCCCGTCGTTTTCTCG12.941176470588235No Hit
GCTCTCGGAAGTATGTCTCGTGTTAC12.941176470588235No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)