FastQCFastQC Report
Sun 15 May 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.Partitivirus_2_segment_2.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.Partitivirus_2_segment_2.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171
Sequences flagged as poor quality0
Sequence length16-34
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATTGCTCGAATTCTCACCGGGA158.771929824561402No Hit
TAGAGAAAACTCAGGAGCAG84.678362573099415No Hit
TTAACCTCAGGAGGCTTCGTC74.093567251461988No Hit
TTTCACCCGCGACTGCAAAATGGA63.508771929824561No Hit
TCACCCGCGACTGCAAAATGGAT63.508771929824561No Hit
TTCGATTGCTCGAATTCTCACCG52.923976608187134No Hit
TCGATTGCTCGAATTCTCACCGGG52.923976608187134No Hit
TTTTCACCCGCGACTGCAAAATGGA52.923976608187134No Hit
TCGATTGCTCGAATTCTCACCGG52.923976608187134No Hit
TCGTTCCAGAACAGTCCG42.3391812865497075No Hit
TCTTGGTTACTCCCATAACGGTC42.3391812865497075No Hit
TTTCACCCGCGACTGCAAAA31.7543859649122806No Hit
TCTGCGTCGGGGAACCAACGGCG31.7543859649122806No Hit
TTCGGTCGGATCAAGGATCTCC31.7543859649122806No Hit
TTTCACCCGCGACTGCAAAATG31.7543859649122806No Hit
TTCCTTAAACTGAGCATCTTCGT21.1695906432748537No Hit
TTCGTCAGATCCCAACGATGT21.1695906432748537No Hit
TAAGGAATTAACCTCAGGAGGCT21.1695906432748537No Hit
TTCGATTGCTCGAATTCTCACCGGGA21.1695906432748537No Hit
TTTCACCCGCGACTGCAAAATGGT21.1695906432748537No Hit
TTGAGGAGTCGGAGCGGAGC21.1695906432748537No Hit
TGGCCAGACCAGAACCACATG21.1695906432748537No Hit
TTCAGGTGCGACGTTTGAGGGT21.1695906432748537No Hit
TCCGAGATCGGCAAGGACGGA21.1695906432748537No Hit
TTTTCACCCGCGACTGCAAAATGG21.1695906432748537No Hit
TTCTTCTTGGGTTGCTGCGCG10.5847953216374269No Hit
TACTCCCATAACGGTCGTAACGGA10.5847953216374269No Hit
TTGGCCAGACCAGAACCACATG10.5847953216374269No Hit
TCGGCAAGGACGGATTGGGGA10.5847953216374269No Hit
TTTTCACCCGCGACTGCAAAAT10.5847953216374269No Hit
TGAGAAAAACTTCTCCATGGTGACT10.5847953216374269No Hit
TGTTCTCACAACAGCAACGGTC10.5847953216374269No Hit
TGGCCGTGACTAGGGTAACCCC10.5847953216374269No Hit
TTCACCCGCGACTGCAAAATGGA10.5847953216374269No Hit
TCGAGACCGGAAATGACATCGTTGATTG10.5847953216374269No Hit
TTCGGAGGTCGTCTGGCTTC10.5847953216374269No Hit
TGGATTGAACCACGTTGCAACC10.5847953216374269No Hit
TCACAAGGACGACAGGGTTTT10.5847953216374269No Hit
TAACCGGACTACTTCCAATCA10.5847953216374269No Hit
TAGGGAGCCACATCCGCTTGACAGG10.5847953216374269No Hit
TCCCACGAGAGCGGAGATCTTG10.5847953216374269No Hit
TCTTGGTTACTCCCATAACGGTCGA10.5847953216374269No Hit
TTGCTCGAATTCTCACCGGGATGGC10.5847953216374269No Hit
TCACCCGCGACTGCAAAATGGACA10.5847953216374269No Hit
TAACCGGACTACTTCCAATCACCT10.5847953216374269No Hit
TAATTGAGGATGTTAA10.5847953216374269No Hit
TCACCGGGATGGCCGTGACTAGGG10.5847953216374269No Hit
TGCCCTTCGGTCGGATCAAGGA10.5847953216374269No Hit
TTCACCCGCGACTGCAAAATGGATTGAACCACGT10.5847953216374269No Hit
TTAACCTCAGGAGGCTTCGTCAG10.5847953216374269No Hit
TAGGGAGCCACATCCGCTTGAC10.5847953216374269No Hit
TTGGTTACTCCCATAACGGTCGTAA10.5847953216374269No Hit
TATGACTTTCCAGGTCGGTAACCGG10.5847953216374269No Hit
TAGAACTCGTCGAGACCGGAA10.5847953216374269No Hit
CAGATCCCAACGATGTCACAAG10.5847953216374269No Hit
CCCATAACGGTCGTAACGGTCGACA10.5847953216374269No Hit
TAGAAACCGGAAAGAGGGAAGCAACT10.5847953216374269No Hit
TGCAGATTGAGGATGTTAACGC10.5847953216374269No Hit
TGTAGAAACCGGAAAGAGGGAA10.5847953216374269No Hit
TGTGCCGTGTTCGGAGGTCGTCTGGC10.5847953216374269No Hit
TCGATTGCTCGAATTCTCACAG10.5847953216374269No Hit
TGCACTTGGAAGAAAGACATCTGA10.5847953216374269No Hit
TAACCCCATAGTCACCGGTGTAGAAACCA10.5847953216374269No Hit
TTGCCAGGTTGAGGAGTCGGAGC10.5847953216374269No Hit
TATGCCCTTCGGTCGGATCAAGG10.5847953216374269No Hit
CGAGACCGGAAATGACATCG10.5847953216374269No Hit
TTGCTCGAATTCTCACCGGGATGG10.5847953216374269No Hit
CACCCGCGACTGCAAAATGG10.5847953216374269No Hit
TGGTTACTCCCATAACGGTCGTC10.5847953216374269No Hit
TCCAGAACAGTCCGAGATCGGC10.5847953216374269No Hit
TTTGGACTGATCCTTTACACC10.5847953216374269No Hit
TGCCAAAATGTGCCGTGTTCGGA10.5847953216374269No Hit
TTGCCAAAATGTGCCGTGTTCG10.5847953216374269No Hit
TAACAAGGACGACAGGGTTTT10.5847953216374269No Hit
TAACCTCAGGAGGCTTCGTC10.5847953216374269No Hit
TTTTCACCCGCGACTGCA10.5847953216374269No Hit
TACCGAGGGCGGGAACCGAAAACTC10.5847953216374269No Hit
TAGTCATCAGGCTCGGATGTGC10.5847953216374269No Hit
CTTGGTTACTCCCATAACGGTC10.5847953216374269No Hit
CCCGCGACTGCAAAATG10.5847953216374269No Hit
TCTGCGTCGGGGAACCAACGGC10.5847953216374269No Hit
TTCGATTGCTCGAATTCTCACCGGG10.5847953216374269No Hit
TTGGAGGCAGAGTCGGA10.5847953216374269No Hit
TTTTCACCCGCGACTGCAAAATA10.5847953216374269No Hit
TGTAGAAACCGGAAAGAGGGAAGCA10.5847953216374269No Hit
TACCGAGGGCGGGAACCGAAAACT10.5847953216374269No Hit
TTTCCGAGACCAGGGTTCAACTT10.5847953216374269No Hit
TTCGGGACAAAGTTGAAATTCAGGTG10.5847953216374269No Hit
TGGCCAGACCAGAACCACATGAGAAAAAA10.5847953216374269No Hit
TCACCCGCGACTGCAAAATGGA10.5847953216374269No Hit
TTTTCACCCGCGACTGCAAAATG10.5847953216374269No Hit
TTCGTCAGATCCCAACGATGTC10.5847953216374269No Hit
TTGAGGAGTCGGAGCGGAGCGA10.5847953216374269No Hit
TGCAGATTGAGGATGTTAACGCT10.5847953216374269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAGC50.044.017
GTCGGAG50.044.016
Produced by FastQC (version 0.11.5)