FastQCFastQC Report
Sun 15 May 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.Partitivirus_2_segment_2.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.Partitivirus_2_segment_2.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50
Sequences flagged as poor quality0
Sequence length16-35
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTGCAGTCGCGGGTGAAAACC48.0No Hit
TGACATCGTTGGGATCTGACGA24.0No Hit
TCTCGGACTGTTCTGGAACGACC24.0No Hit
TGCCGATCTCGGACTGTTCTGGA24.0No Hit
TTGAACCCTGGTCTCGGAAACGA24.0No Hit
TTAATTCCTTAGTGATGGTGACA24.0No Hit
TTCTGCTTAAGGATTACACAACCACC24.0No Hit
TGGTCCCCAATCCGTCCTTGCCGATCTCGGACTGT24.0No Hit
TCAAGCGGATGTGGCTCCCTA24.0No Hit
TTAACATCCTCAATCTGCACATC12.0No Hit
GTGACATCGTTGGGATCTGACGAAGCCTCCT12.0No Hit
TTTTCGGTTCCCGCCCTCGGTA12.0No Hit
TTTTTCTCATGTGGTTCTGGTCTGG12.0No Hit
TAACCAAGATCTCCGCTCTCGT12.0No Hit
TTGTCTCACTCGTTTTGCTGCGAC12.0No Hit
ACCCTGTCGTCCTTGTGA12.0No Hit
GGGTCTTCCACTTGGCGACTTCTCGTCGGTTT12.0No Hit
TCCGTCCTTGCCGATCTCGGACT12.0No Hit
CGCTGGATACGAAGATGCTCAGTTTAAGGAACA12.0No Hit
TTTTCTCATGTGGTTCTGGTCTGGCCAACA12.0No Hit
TCCTTGTGACATCGTTGGGA12.0No Hit
TTGCAGTCGCGGGTGAAAACC12.0No Hit
TTGCCGATCTCGGACTGTTCTG12.0No Hit
TTACCGTTGCTGTTGTGAGAACA12.0No Hit
TTACTAATGATGGTTCGACTGTTGAC12.0No Hit
TAAGGATTACACAACCACCGTCT12.0No Hit
TTGTCTCACTCGTTTTGCTGCGACTCGTGGTATA12.0No Hit
TTGTGACATCGTTGGGATCTGACGA12.0No Hit
TTCCGACTCTGCCTCC12.0No Hit
TAACCAAGATCTCCGCTCTCG12.0No Hit
TCGGAAACGAGTCACCATGGAGAA12.0No Hit
CTGTTCTGGAACGACCCTCAAACGTCGCACC12.0No Hit
CCTTGTGACATCGTTGGG12.0No Hit
TTTTTCAGACTGGCGCTGGTGAACC12.0No Hit
TTGATTTCCTCTTCACGCCAAAAT12.0No Hit
TTTCCGGTCTCGACGAGTTCTATC12.0No Hit
GACATCGTTGGGATCTGACGA12.0No Hit
TTCTTTTCCCTGAACCCTGGACC12.0No Hit
CTCTGATCGATTCTTTTCCCTGAACCCTGGACC12.0No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTCA50.017.06
TCCTCAA50.017.07
TGCACAT50.017.016
AATCTGC50.017.012
CATCCTC50.017.05
CAATCTG50.017.011
TCAATCT50.017.010
ACATCCT50.017.04
TCTGCAC50.017.014
TAACATC50.017.02
CCTCAAT50.017.08
TTAACAT50.017.01
CTGCACA50.017.015
GCACATC50.017.017
ATCTGCA50.017.013
CTCAATC50.017.09
AACATCC50.017.03
Produced by FastQC (version 0.11.5)