FastQCFastQC Report
Sun 15 May 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.Partitivirus_3_segment_1.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.Partitivirus_3_segment_1.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35
Sequences flagged as poor quality0
Sequence length15-35
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGATTGATCGCCTGGCCCC38.571428571428571No Hit
AGCCTTCGTCAATCACCC25.714285714285714No Hit
AGCAGGCTATGGATATCTCGG25.714285714285714No Hit
CACATGTCTGAGCCTCTCCCT25.714285714285714No Hit
ATTGACAGTTTGATTAATC25.714285714285714No Hit
TACAGATCCATTCGTCCATGAACTTCTCAGAGAA12.857142857142857No Hit
CCAGGACACTGTTTACGAACAACCC12.857142857142857No Hit
ACATGTCTGAGCCTCTCCCT12.857142857142857No Hit
GATTGATCGCCTGGC12.857142857142857No Hit
ACGCCCGATGGACATCGACTCACCCT12.857142857142857No Hit
TTTCTCTTGGCCTTGTATTTG12.857142857142857No Hit
ATCCATTCGTCCATGAACTTCTCAGAGAACGGCT12.857142857142857No Hit
CTCCATCCTCAGTAGCAGGCTATGGATATCTCGGC12.857142857142857No Hit
CTCACCCTTTCCTAAA12.857142857142857No Hit
AAGGATTGATCGCCTGGCCCCTC12.857142857142857No Hit
TGTCTAATAGGTGCGTCCTATAACAGAATTACC12.857142857142857No Hit
CAAGATTCGACATGTATGGGG12.857142857142857No Hit
GAACACGTCGATAATACCGTCGGACTGTATGGACT12.857142857142857No Hit
AACGCCCGATGGACATCGACTCACCCTTTCCT12.857142857142857No Hit
ATGGACATCGACTCACCCTTTCC12.857142857142857No Hit
GGATTGATCGCCTGGCCCCT12.857142857142857No Hit
ATTCCATCTTCGCTGCAGAAT12.857142857142857No Hit
TTGGCCTTGTATTTGTTGTAACCCT12.857142857142857No Hit
GAAGGATTGATCGCCTGGCCC12.857142857142857No Hit
AGACTCTTCTTTGATCAATTTGGACTGACCCT12.857142857142857No Hit
AAGCTTCAAGACGCACACAGC12.857142857142857No Hit
TGAAGGATTGATCGCCTGGCCC12.857142857142857No Hit
AAGGATTGATCGCCTGGCCC12.857142857142857No Hit
TCTTAATTGAAGGATTGATCGCCTGGA12.857142857142857No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)