FastQCFastQC Report
Sun 15 May 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.Partitivirus_3_segment_2.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.Partitivirus_3_segment_2.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64
Sequences flagged as poor quality0
Sequence length16-35
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGAACTTCTTTACCCACCGATGCC57.8125No Hit
AACCCACAGAACTTCTTTACCCACCGATGCC46.25No Hit
CCCACAGAACTTCTTTACCCACCGATGCC34.6875No Hit
CACAGAACTTCTTTACCCACCGATGCC34.6875No Hit
AACCGATCTTGTCGAACC23.125No Hit
ACCCACAGAACTTCTTTACCCACCGATG23.125No Hit
TAACCCACAGAACTTCTTTACCCACCGATGCC23.125No Hit
GCCGGGATGTCTACGACTGATGAAGCCATGG23.125No Hit
CAGTAACCCACAGAACTTCTTTACCCACCGATGCC23.125No Hit
AACGCGTGGTCTTATGAGGAT23.125No Hit
CGGATCCTACAAAACAGC23.125No Hit
CTCAGAATCTGCAAACTTTGGTGTCGTACACA23.125No Hit
ACAGAACTTCTTTACCCACCGATGC23.125No Hit
ACCCACAGAACTTCTTTACCCACCGATGCC23.125No Hit
CAGAACTTCTTTACCCACCGATGCC23.125No Hit
GTAACCCACAGAACTTCTTTACCCACCGATGCC11.5625No Hit
CAGAACTTCTTTACCCACC11.5625No Hit
GATATCACCCATACGAACACCGATGTATACG11.5625No Hit
TCGCACGAGACTTCAAGCTC11.5625No Hit
TAACCCACAGAACTTCTTTACCCACCGATGCA11.5625No Hit
CCACAGAACTTCTTTACCCACCGATGCC11.5625No Hit
AATTGCAGCACGTGGTTTCTCTGGAAGTATTGAAC11.5625No Hit
CAACCGATCTTGTCGAACCCGCT11.5625No Hit
ACCCACAGAACTTCTTTACCCACCGA11.5625No Hit
CTTGGTTAATGGCGTAGGAGC11.5625No Hit
AGTAACCCACAGAACTTCTTTACCCACCGATGCC11.5625No Hit
AGTAACCCACAGAACTTCTTTACCCACCGATG11.5625No Hit
CAGTAACCCACAGAACTTCTTTACCCACCGATGC11.5625No Hit
ATATGTTATGAAGTCGAGGAT11.5625No Hit
TAGTATCCTGCATCCCTGAACC11.5625No Hit
ACCAGAGGCTGCACCA11.5625No Hit
ACGTGGTTTCTCTGGAAGT11.5625No Hit
CACTATTAGAAACCTCCCGGGCTTGCG11.5625No Hit
CTGCAATTGCAGCACGTGGTTTCTCTGGAAGTATT11.5625No Hit
CAGAACTTCTTTACCCACCGATGC11.5625No Hit
TGTCTGCTGACACTATTAGAAACCTCCCGGGCTT11.5625No Hit
GTTTATGACTCTGTTCAGCCGGGCGCTCAAT11.5625No Hit
ACCCACAGAACTTCTTTACCCACCGATGCAT11.5625No Hit
AACCGATCTTGTCGAACCCGCTGACA11.5625No Hit
GCGCAACTACAAGGACCGGACC11.5625No Hit
ACAGAAACCCACAGAACTTCTTTACCAACCGATGC11.5625No Hit
ACAGAACTTCTTTACCCACCGATG11.5625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTACC50.019.09
TTACCCA50.019.011
CACCGAT50.019.016
CCCACCG50.019.014
AGAACTT50.019.02
ACCGATG50.019.017
ACTTCTT50.019.05
AACTTCT50.019.04
CCACCGA50.019.015
GAACTTC50.019.03
TTCTTTA50.019.07
ACCCACC50.019.013
TACCCAC50.019.012
CGATGCC50.019.019
CAGAACT50.019.01
TCTTTAC50.019.08
TTTACCC50.019.010
CCGATGC50.019.018
CTTCTTT50.019.06
Produced by FastQC (version 0.11.5)