FastQCFastQC Report
Sun 15 May 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.Sobemovirus_segment_1.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.Sobemovirus_segment_1.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346
Sequences flagged as poor quality0
Sequence length15-33
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGAGAACCGTCCGACAAC154.335260115606936No Hit
CTCCTGGATCCGACACTGCTG144.046242774566474No Hit
CTCCGGTAGACCATGCAGCCT102.8901734104046244No Hit
TAAACTCAACCTCGGCTGACC92.601156069364162No Hit
TCATGAGCATGCCTGACGCCT82.312138728323699No Hit
TTAGAGCTCACCCCACCGCCT61.7341040462427744No Hit
CTACTCGCCTCCCTTTCTTCA51.4450867052023122No Hit
AGCCAAGACATGGTCCGGACC41.1560693641618496No Hit
ATGCCTGACGCCTACTCGCCT41.1560693641618496No Hit
CAACGGCTCCGGACTCACGCC41.1560693641618496No Hit
CGATCACTACCCGCTCCATGC30.8670520231213872No Hit
ACCATATCCGTTGCGACCTCGT30.8670520231213872No Hit
TTGAGACTTATCGCCTGCTGG30.8670520231213872No Hit
TCTCCTGGATCCGACACTGCT30.8670520231213872No Hit
ATCTCCTGGATCCGACACTGC30.8670520231213872No Hit
ATCCGTTGCGACCTCGACCCG30.8670520231213872No Hit
CAGAGAACCGTCCGACAACAT30.8670520231213872No Hit
AATCACTACCCGCTCCATGCC30.8670520231213872No Hit
ATACAGAGAACCGTCCGACAA30.8670520231213872No Hit
CAATCACTACCCGCT30.8670520231213872No Hit
AGAGAACCGTCCGACAACAT20.5780346820809248No Hit
AGAGAACCGTCCGACAACA20.5780346820809248No Hit
CCCAGGGGACTGGTCTTGCCT20.5780346820809248No Hit
CAACTCCATGCCGGCTCCCCG20.5780346820809248No Hit
AACTCCATGCCGGCTC20.5780346820809248No Hit
CAGGGCGCCCTCCGAACGCGA20.5780346820809248No Hit
TACCTGTAGGCATGGCGC20.5780346820809248No Hit
AGAACAATCTCCACATCCGCC20.5780346820809248No Hit
CGCATTGATGGGAGGCGGCTT20.5780346820809248No Hit
TCCGTTGCGACCTCGACCCGT20.5780346820809248No Hit
ACAGAGAACCGTCCGACAACA20.5780346820809248No Hit
AACTTCTCCGGTAGACCATGC20.5780346820809248No Hit
ACAGAGAACCGTCCGACAAC20.5780346820809248No Hit
TCGGACGCCATCATCTCCTGG20.5780346820809248No Hit
CACAACTCCATGCCGGCTCCC20.5780346820809248No Hit
TGGCGTGCTGCGTAGCTCTCC20.5780346820809248No Hit
ATGACCATATCCGTTGCGACC20.5780346820809248No Hit
CCCAGATGCCGGACGACC20.5780346820809248No Hit
TTCAACCCTCTCACTAGCGCC20.5780346820809248No Hit
CCAGGGGACTGGTCTTGCCT20.5780346820809248No Hit
CACATTCGCGTCCGACCGATC20.5780346820809248No Hit
CCAACATCAAACCAGTACCTG20.5780346820809248No Hit
CGGCTCCGGACTCACGCC20.5780346820809248No Hit
AACTCAACCTCGGCTGAC20.5780346820809248No Hit
CCATTTCACTATCGTTCGGGA20.5780346820809248No Hit
AGACATGGTCCGGACCA20.5780346820809248No Hit
GCACACATTCGCGTCCGACCG20.5780346820809248No Hit
TTCGCGTCCGACCGATCAGCC20.5780346820809248No Hit
GGATCCGACACTGCTGCTTCC20.5780346820809248No Hit
GTTAGAGCTCACCCCACCGCC20.5780346820809248No Hit
ACCGCTTTCAACCCTCTCACT20.5780346820809248No Hit
CCACTCAACCTCGGC20.5780346820809248No Hit
TTCCTGGATCCGACACTGCTG20.5780346820809248No Hit
CCATTGGTAGAGATTGTGCACCC20.5780346820809248No Hit
AGAGAACCGTCCGACAACATC20.5780346820809248No Hit
CGGCTTCTCCCTCTGATAAGG20.5780346820809248No Hit
AGCCAAGACATGGTCCGGACCA20.5780346820809248No Hit
CGGTAGACCATGCAGCCTGGC20.5780346820809248No Hit
CTCCGAAGCTTCAGGTCTGGT20.5780346820809248No Hit
TTCCGCGCCTCGGACGCCATC20.5780346820809248No Hit
CTTCTCCCTCTGATAAGGCACC10.2890173410404624No Hit
CCCACAGTCGGCTTCTCCCTC10.2890173410404624No Hit
AGAACAATCTCCACATCCGC10.2890173410404624No Hit
CACATCCGCCCAGAGTCTGTC10.2890173410404624No Hit
TTTCGACCTCCTCTTCTTGAG10.2890173410404624No Hit
CGCTCCATGCCGGTG10.2890173410404624No Hit
TCCTGGATCCGACACTGC10.2890173410404624No Hit
ATCAGGGCGCCCTCCGAACGC10.2890173410404624No Hit
CAAGACATGGTCCGGACCA10.2890173410404624No Hit
CAACGGGGTTAGAGCTCACCC10.2890173410404624No Hit
GAACTCCTCCGCCCGATCACC10.2890173410404624No Hit
GACCCTGTCATCATACG10.2890173410404624No Hit
TACAACATCCCCGTGGTCCCT10.2890173410404624No Hit
CCATTCGTCTTGCCCCCC10.2890173410404624No Hit
ACGGACTCTAGGGTAACG10.2890173410404624No Hit
ATAAAGGCCTGCGGA10.2890173410404624No Hit
CTTCTCCGGTAGACCATGCACC10.2890173410404624No Hit
GCCAAGACATGGTCCGGACCA10.2890173410404624No Hit
AAACCAGTACCTGTAGGCATG10.2890173410404624No Hit
CAACGGCTCCGGACTCACGCCC10.2890173410404624No Hit
CACCAGCAGACTGTCCAGTTC10.2890173410404624No Hit
CTACCGCTTTCAACCCTCTCA10.2890173410404624No Hit
AACGCCTCCACTCTCC10.2890173410404624No Hit
TAGGGTAACGGGCATCAGTGG10.2890173410404624No Hit
AAGCGGCTCCGGACTCA10.2890173410404624No Hit
CCGCCATTCGTCTTGCCCCC10.2890173410404624No Hit
ACTACCGCTTTCAACCCTCTC10.2890173410404624No Hit
ATTCGTCTTGCCCCCCCGCTG10.2890173410404624No Hit
CCTCCCTTTCTTCATCGCAT10.2890173410404624No Hit
ATACAGAGAACCGTCCGACAAC10.2890173410404624No Hit
TCGTCTTGAGCGAATGAGGCT10.2890173410404624No Hit
CATGCCTGACGCCTACTCGCCC10.2890173410404624No Hit
AAACAGAGCCGGGTCATCATA10.2890173410404624No Hit
ACTGATCAAAGCCCACCCCGT10.2890173410404624No Hit
ACTGAGGGTCTGGTCCG10.2890173410404624No Hit
AGCGAACTCCTGACAATGTCC10.2890173410404624No Hit
TTCCGCGCCTCGGACGCC10.2890173410404624No Hit
AAAACAATCTCCACATCCGCC10.2890173410404624No Hit
AGGTGCCGTCCAATCCC10.2890173410404624No Hit
GCGCACACATTCGCGTCCGAC10.2890173410404624No Hit
CACGCCCATCCGCATGCAAGC10.2890173410404624No Hit
CGAACTCCTGACAATGTCCAT10.2890173410404624No Hit
ACCGATCAGCCAAGAC10.2890173410404624No Hit
TGAGCGAACTCCTGACAA10.2890173410404624No Hit
TGAGACTTATCGCCTGCTGG10.2890173410404624No Hit
ACCGGGGTTAGAGCTCACCC10.2890173410404624No Hit
GATCACTACCCGCTCC10.2890173410404624No Hit
CCAAGACATGGTCCGGACCA10.2890173410404624No Hit
CCGGTAGACCATGCAGCC10.2890173410404624No Hit
GATCACTACCCGCTCCATGCCC10.2890173410404624No Hit
CTGAGGGTCATGAGCATGCC10.2890173410404624No Hit
TCCGGACTCACGCCCATCCGC10.2890173410404624No Hit
TCCGGTAGACCATGCAGCCTG10.2890173410404624No Hit
CATCAACCCATCCGTCCCGCTT10.2890173410404624No Hit
TGCCCCATCTTCGTTGCGAT10.2890173410404624No Hit
TGAGCATGCCTGACGCCT10.2890173410404624No Hit
CAACGATCCCCATAGAAGCATAAAACTCTACTG10.2890173410404624No Hit
AGTACCTGTAGGCAT10.2890173410404624No Hit
GGTAGACCATGCAGCCTGGC10.2890173410404624No Hit
ATAAGGCAAACCAGGGGAC10.2890173410404624No Hit
AATGGCTCCGGACTCACGCCC10.2890173410404624No Hit
ATGTCTAGGGTGAATCAGGGTG10.2890173410404624No Hit
GACATGGTCCGGACC10.2890173410404624No Hit
TTGCGACCTCGACCCG10.2890173410404624No Hit
ACCACTCCCCCACAGTCGGCT10.2890173410404624No Hit
TCCATTTCACTATCGTTCGGG10.2890173410404624No Hit
TCAAGAACAATCTCCACATCC10.2890173410404624No Hit
CCAGATGCCGGACGACCG10.2890173410404624No Hit
TCCGACAACATCACCACCACT10.2890173410404624No Hit
TTAAGACTTATCGCCTGCTGG10.2890173410404624No Hit
CACTATCGTTCGGGACAAGGG10.2890173410404624No Hit
TCTCCGGTAGACCATGCAGCC10.2890173410404624No Hit
GTCCGGACCAATGAGGTAATC10.2890173410404624No Hit
CCATTCGTCTTGCCCCC10.2890173410404624No Hit
CGCTCCGCCAACCCCGCCAGC10.2890173410404624No Hit
CCGCCATTCGTCTTGCCCCCC10.2890173410404624No Hit
CCCCCGATGACCACGAAAACCCCGTCT10.2890173410404624No Hit
AGACATGGTCCGGACC10.2890173410404624No Hit
GTGGTCCGAGAGAATGAGCTC10.2890173410404624No Hit
GGCTTGGATCCTCTGAGG10.2890173410404624No Hit
TAGAACGGATACGGCCTTAGCA10.2890173410404624No Hit
TGAGACTTATCGCCTGCTG10.2890173410404624No Hit
TTCGCGTCCGACCGATCAGCCT10.2890173410404624No Hit
CTAGAGCACCGACCTTGGCCT10.2890173410404624No Hit
GCCAAGACATGGTCCGGAC10.2890173410404624No Hit
AAGGGCTCCAAAGAAAC10.2890173410404624No Hit
CTCCGGTAGACCATGCAGCCTT10.2890173410404624No Hit
CATCTTCGTTGCGATAGTTCC10.2890173410404624No Hit
TGGTAGAGATTGTGCACACA10.2890173410404624No Hit
CGGACTCACGCCCATCCGC10.2890173410404624No Hit
CAAAAACTCCTCCGCC10.2890173410404624No Hit
TTCCGCGCCTCGGACG10.2890173410404624No Hit
CTCCCGTTCTTCCGCGCCTCG10.2890173410404624No Hit
GCGAACTCCTGACAATGTCC10.2890173410404624No Hit
CCATATCCGTTGCGACCTCG10.2890173410404624No Hit
TCATACAACATCCCCGTGGTC10.2890173410404624No Hit
TCCGGTAGACCATGCAGCCT10.2890173410404624No Hit
CGACATACGCGTCCCACCCAC10.2890173410404624No Hit
ATACAACATACCCGTGGTCCC10.2890173410404624No Hit
CATGAGCATGCCTGACGCCT10.2890173410404624No Hit
AACTCCATGCCGGCTCCC10.2890173410404624No Hit
ACTCAACCTCGGCTGACCCGG10.2890173410404624No Hit
AAACTTCTCCGGTAGACCATG10.2890173410404624No Hit
CCAAGACATGGTCCGGACC10.2890173410404624No Hit
TTGGGACAAGGGCGC10.2890173410404624No Hit
GACATGGTCCGGACCAATGA10.2890173410404624No Hit
AGCGTAGAAATGGTCCG10.2890173410404624No Hit
CGATACAGAGAACCGTCCGAC10.2890173410404624No Hit
CCACGTACTGAGGGTC10.2890173410404624No Hit
AAGAAAACTTTGGATTCTCCT10.2890173410404624No Hit
AATCCGGCTCCCGTTCTTCCG10.2890173410404624No Hit
ACATGGTCCGGACCA10.2890173410404624No Hit
AGACCACACTCGATCTCTTTGTCGTTCTG10.2890173410404624No Hit
AAACTTTGGATTCTCCTTC10.2890173410404624No Hit
CTCTAGAGCACCGACCTTGGC10.2890173410404624No Hit
TTCGTCTTGCCCCCC10.2890173410404624No Hit
ATAAAACTCTACTGTCTGGAC10.2890173410404624No Hit
CTCACCAGATCAATGTTCACCAAT10.2890173410404624No Hit
TATAAGGGCTCCAAAG10.2890173410404624No Hit
CGGCTCCGGACTCACGC10.2890173410404624No Hit
GCATGCCTGACGCCTACTCGC10.2890173410404624No Hit
TCCAGTTCGCAGGCCGGTTCT10.2890173410404624No Hit
AGACATGGTCCGGAC10.2890173410404624No Hit
TTCTTCCGCGCCTCGGACG10.2890173410404624No Hit
CCATTCGTCTTGCCCCCCCGC10.2890173410404624No Hit
ATGGTCCGAGAGAATGAGCTC10.2890173410404624No Hit
CGACACTGCTGCTTCCGAGTCCA10.2890173410404624No Hit
ACAATCTCCACATCCGCCCAG10.2890173410404624No Hit
GAGTGAACTCAACCTCGGCT10.2890173410404624No Hit
CAGAAAGTCATCAGGTAGAGT10.2890173410404624No Hit
GTGCCGTCCAATCCCAGGC10.2890173410404624No Hit
CGTTTCGACCTCCTCTTC10.2890173410404624No Hit
CCGGTTCTATTTCTCCTGGGT10.2890173410404624No Hit
GAGACTTATCGCCTGCTGGCT10.2890173410404624No Hit
CCCGCCATTCGTCTTGCCCCCT10.2890173410404624No Hit
CTGAGGGTCATGAGCATGCCT10.2890173410404624No Hit
CATCATCTCCTGGATCCGACA10.2890173410404624No Hit
GGAAGCGGCTCCGGACT10.2890173410404624No Hit
TGGTCCGGACCAATGAGGTAAT10.2890173410404624No Hit
AACTCCATGCCGGCTCCCC10.2890173410404624No Hit
CAACTCCATGCCGGCTCCCC10.2890173410404624No Hit
CCGACACTGCTGCTTCCGAG10.2890173410404624No Hit
GCGTATGCACCAGCAGACTGT10.2890173410404624No Hit
ACAAGGGCGCCCTCCGAACGC10.2890173410404624No Hit
AAACAACGGGGTTAGAGCTC10.2890173410404624No Hit
CGCAGGCCGGTTCTATTTCTC10.2890173410404624No Hit
CTGGATCCGACACTGCTGCTT10.2890173410404624No Hit
CAGTACCTGTAGGCATG10.2890173410404624No Hit
ATCAGCCAAGACATGGTCCGG10.2890173410404624No Hit
ATATGCCAGGCCACCTGCAC10.2890173410404624No Hit
AATCTCCACATCCGCCCA10.2890173410404624No Hit
TGCCCCCATCTTCGTTGCGAT10.2890173410404624No Hit
TGAGCATGCCTGACGC10.2890173410404624No Hit
AGCTTCAGGTCTGGTCCGGTT10.2890173410404624No Hit
GGCGAACTCCTGACAATGTCC10.2890173410404624No Hit
AGCGTAGAAATGGTCCGAGAG10.2890173410404624No Hit
ATCCGGCTCCCGTTCTTCCGC10.2890173410404624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTCGT50.091.016
Produced by FastQC (version 0.11.5)