FastQCFastQC Report
Sun 15 May 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.Sobemovirus_segment_1.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.Sobemovirus_segment_1.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230602
Sequences flagged as poor quality0
Sequence length15-35
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTGTAGGCGACGACAATACC2518510.921414384957632No Hit
ATTGAGTGTAGGCGACGACAATACC178347.733671000251515No Hit
AGTGTAGGCGACGACAATACC154426.696385981040928No Hit
TTGAGTGTAGGCGACGACAATACC145196.296129261671625No Hit
ATTGAGTGTAGGCGACGACAATACCAGGAGTCCC120095.207673827633759No Hit
CTGAATTGAGTGTAGGCGACGACAATACC107304.653038568616057No Hit
AATTGAGTGTAGGCGACGACAATACC75393.2692691303631367No Hit
AATTGAGTGTAGGCGACGACAATACCAGGAGTCCC69713.02295730305895No Hit
TTGAGTGTAGGCGACGACAATACCAGGAGTCCC68762.981760782647158No Hit
GAATTGAGTGTAGGCGACGACAATACC60932.6422147249373378No Hit
CCCGAGCGCGCGTGGCC46512.0168949098446673No Hit
CGAGCGCGCGTGGCC46442.013859376761693No Hit
GAGTGTAGGCGACGACAATACC42891.8599144846965767No Hit
ATTGAGTGTAGGCGACGACAATACCAGGAGTCC38251.65870200605372No Hit
CCGAGCGCGCGTGGCC36621.5880174499787514No Hit
TGAATTGAGTGTAGGCGACGACAATACC33961.4726671928257344No Hit
TTGAGTGTAGGCGACGACAATACCAGGAGTCC25251.0949601477870963No Hit
GTGTAGGCGACGACAATACC24281.0528963322087406No Hit
AATTGAGTGTAGGCGACGACAATACCAGGAGTCC23761.0303466578780758No Hit
TGAGTGTAGGCGACGACAATACCAGGAGTCCC21930.9509891501374663No Hit
ACTGAATTGAGTGTAGGCGACGACAATACC19930.8642596334810626No Hit
ATTGAGTGTAGGCGACGACAATA16430.7124829793323562No Hit
ATTGAGTGTAGGCGACGACAATACCAGGAGTCCCG15970.6925351905013833No Hit
TGAGTGTAGGCGACGACAATAC13020.5646091534331878No Hit
TGAGTGTAGGCGACGACAATA12650.5485641928517532No Hit
CTGAATTGAGTGTAGGCGACGACAATACCAGGA12180.5281827564374983No Hit
GAATTGAGTGTAGGCGACGACAATACCAGGAGTCC11900.5160406241056018No Hit
GACTGAATTGAGTGTAGGCGACGACAATACC11410.49479189252478295No Hit
ATTGAGTGTAGGCGACGACAATAC10330.44795795353032497No Hit
TGAGTGTAGGCGACGACAATACCAGGAGTCC9240.400690366952585No Hit
TTGAGTGTAGGCGACGACAATA8950.3881145870374064No Hit
GGAGTCCCGAGCGCGCGTGGCC8470.3672995030398696No Hit
ACAGACTGAATTGAGTGTAGGCGACGACAATACC8200.35559101829125506No Hit
AGACTGAATTGAGTGTAGGCGACGACAATACC8130.35255548520828095No Hit
GTCCCGAGCGCGCGTGGCC7790.3378114673766923No Hit
ATTGAGTGTAGGCGACGACAATACCAGG7720.33477593429371816No Hit
ATTGAGTGTAGGCGACGACAATACCAGGA7710.3343422867104362No Hit
TTGAGTGTAGGCGACGACAATACCAGGAGTCCCG7510.32566933504479584No Hit
CCCGAGCGCGCGTGGC7440.3226338019618217No Hit
AGGAGTCCCGAGCGCGCGTGGCC7200.3122262599630532No Hit
TTGAGTGTAGGCGACGACAATAC7060.306155193797105No Hit
CCGAGCGCGCGTGGCCC7050.30572154621382297No Hit
CAGGAGTCCCGAGCGCGCGTGGCC6630.28750834771597816No Hit
TCCCGAGCGCGCGTGGCC6400.27753445330049176No Hit
CCGAGCGCGCGTGGC6130.26582596855187723No Hit
CCCGAGCGCGCGTGGCCC5630.24414358938777636No Hit
TGAGTGTAGGCGACGACAATACCAGGAGTCCCG5460.23677158047198202No Hit
TGAGTGTAGGCGACGACAATACCA5060.21942567714070127No Hit
CAGACTGAATTGAGTGTAGGCGACGACAATACC4990.21639014405772714No Hit
CTGAATTGAGTGTAGGCGACGACAATAC4790.20771719239208677No Hit
ATTGAGTGTAGGCGACGACAATACCAGGAGTC4680.20294706897598458No Hit
ATTGAGTGTAGGCGACGACAATACCA4640.20121247864285652No Hit
CTGAATTGAGTGTAGGCGACGACAATACCA4470.1938404697270622No Hit
AATTGAGTGTAGGCGACGACAATACCAGGA4470.1938404697270622No Hit
AATTGAGTGTAGGCGACGACAATACCAGG4390.19037128906080608No Hit
GAATTGAGTGTAGGCGACGACAATACCAGGA4380.18993764147752407No Hit
GTCCCGAGCGCGCGTGGC4370.18950399389424202No Hit
CCAGGAGTCCCGAGCGCGCGTGGCC4350.188636698727678No Hit
CGAGCGCGCGTGGCCC4350.188636698727678No Hit
GAATTGAGTGTAGGCGACGACAATACCAGG3990.1730253857295253No Hit
CTGAATTGAGTGTAGGCGACGACAATACCAGG3970.17215809056296127No Hit
AGCGCGCGTGGCCCT3920.1699898526465512No Hit
ATACCAGGAGTCCCGAGCGCGCGTGGCC3780.16391878648060293No Hit
AGCGCGCGTGGCCCTC3620.15698042514809066No Hit
TTGAGTGTAGGCGACGACAATACCAGG3430.1487411210657323No Hit
TTGAGTGTAGGCGACGACAATACCA3410.14787382589916825No Hit
TGTAGGCGACGACAATACC3370.1461392355660402No Hit
TTGAGTGTAGGCGACGACAATACCAGGAGTC3350.14527194039947616No Hit
AGTGTAGGCGACGACAATAC3140.1361653411505538No Hit
GAGTCCCGAGCGCGCGTGGCC3000.13009427498460552No Hit
GAATTGAGTGTAGGCGACGACAATACCAGGAGT2950.12792603706819541No Hit
AATTGAGTGTAGGCGACGACAATACCAGGAGTC2940.1274923894849134No Hit
AATTGAGTGTAGGCGACGACAATACCAGGAGT2930.12705874190163138No Hit
GAGCGCGCGTGGCCC2910.12619144673506735No Hit
GGAGTCCCGAGCGCGCGTGGC2870.12445685640193928No Hit
CAGGAGTCCCGAGCGCGCGTGGC2790.12098767573568313No Hit
TGAGTGTAGGCGACGACAATACCAGG2700.11708484748614496No Hit
TGAATTGAGTGTAGGCGACGACAATACCAGGA2640.11448296198645284No Hit
GTAGGCGACGACAATACC2610.11318201923660678No Hit
TTACGCCTGAACGCCT2590.11231472407004275No Hit
CGACAATACCAGGAGTCCCGAGCGCGCGTGGCC2560.11101378132019671No Hit
AATTGAGTGTAGGCGACGACAATAC2500.10841189582050459No Hit
GAGTGTAGGCGACGACAATACCAGGAGTCCC2490.10797824823722257No Hit
TACCAGGAGTCCCGAGCGCGCGTGGCC2460.10667730548737653No Hit
TTGAGTGTAGGCGACGACAATACCAGGAGT2400.10407541998768441No Hit
GAATTGAGTGTAGGCGACGACAATACCAGGAGTC2390.1036417724044024No Hit
TGAATTGAGTGTAGGCGACGACAATACCAGG2390.1036417724044024No Hit
ATTGAGTGTAGGCGACGACAATACCAGGAGT2380.10320812482112038No Hit
AATTGAGTGTAGGCGACGACAATACCA2380.10320812482112038No Hit
ATCCGTTCTAATCGTAGTGGTGTACAGACTGA2360.10234082965455635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCCC43150.065.137729
AGTCCCG12800.044.8787529
GGAGTCC55000.033.32861727
AGGAGTC55150.028.50785426
CAGGAGT55900.026.3109125
CCAGGAG55400.024.69756724
GAGTGAA250.00444428120.0221214
TGAAGGC250.00444428120.0221217
AAGGCGA250.00444428120.0221219
AGTGAAG250.00444428120.0221215
GTGAAGG250.00444428120.0221216
TGAGTGA250.00444428120.0221213
GAAGGCG250.00444428120.0221218
ACCAGGA59000.019.87483423
TACCAGG61850.018.17728622
ATACCAG61550.016.5923821
AATACCA63950.014.76469520
GGGATTA902.8319664E-713.3451861
CTGAATT22750.013.066551
GGATTAC1001.0161075E-612.0106682
Produced by FastQC (version 0.11.5)