FastQCFastQC Report
Sun 15 May 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.Sobemovirus_segment_2.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.Sobemovirus_segment_2.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8784
Sequences flagged as poor quality0
Sequence length15-35
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCGTAGGAGTCTGGTCTTGGTCACAGCATC730.8310564663023678No Hit
TCCCGTAGGAGTCTGGTCTTGGTCACAGCATC700.7969034608378871No Hit
AGGCACCGGCGGCGACG650.7399817850637522No Hit
CCCGTAGGAGTCTGGTCTTGGTCACAGCATC580.6602914389799636No Hit
CTCGTGTTTGACCCTCTGCCT460.5236794171220401No Hit
TATATCCACGGGTCGTCTTCG390.44398907103825136No Hit
AGAATGTGGCGTCGGTGACA390.44398907103825136No Hit
CGAAGGCGTTTCAAGAGAGTCGTTGGCACAG380.4326047358834244No Hit
TCAAGAGAGTCGTTGGCACAG360.4098360655737705No Hit
CGGGTGATCAGATTCATGGGCTCTTTTACGGTCTC340.3870673952641166No Hit
CTCTCCCGTAGGAGTCTGGTCTTGGTCACAG320.36429872495446264No Hit
GCACCGGCGGCGACG310.35291438979963574No Hit
GTGGCGGTGCTGTGGGTTTTTCCA290.3301457194899818No Hit
ATCCACGCTCAGACGACA290.3301457194899818No Hit
CAAGAGAGTCGTTGGCACAGC280.31876138433515483No Hit
CGAAGGCGTTTCAAGAGAGTCGTTGGCACA280.31876138433515483No Hit
GGCTCTCCCGTAGGAGTCTGGTCTTGGTCACAG270.3073770491803279No Hit
TTCAAGAGAGTCGTTGGCACA250.2846083788706739No Hit
GCGGCATCAAAACCCTGGCC240.273224043715847No Hit
AAGGCACCGGCGGCGACG240.273224043715847No Hit
CGCGGCGCTGCCGGGGCCC240.273224043715847No Hit
TCTCCCGTAGGAGTCTGGTCTTGGTCACAGCATC230.26183970856102007No Hit
CTCTCCCGTAGGAGTCTGGTCTTGGTCACAGCATC210.23907103825136614No Hit
GTACACGAGCCAGCGTTTCGTCAGCGTTACAATG210.23907103825136614No Hit
GAAGGCGTTTCAAGAGAGTCGTTGGCACAG200.22768670309653918No Hit
GGCGGTGCTGTGGGTTTTTCCA200.22768670309653918No Hit
ACCCTGTTGGGTGGCGGTGCTGTGGGTTTTTCCA200.22768670309653918No Hit
TCGCGGCGCTGCCGGGGC190.2163023679417122No Hit
TTTCAAGAGAGTCGTTGGCAC180.20491803278688525No Hit
TCGCGGCGCTGCCGGGGCCC180.20491803278688525No Hit
CTCCCGTAGGAGTCTGGTCTTGGTC180.20491803278688525No Hit
TGTTGGGTGGCGGTGCTGTGGGTTTTTCCAGGCT170.1935336976320583No Hit
CGAAGGCGTTTCAAGAGAGTCGTTGGCACAGC170.1935336976320583No Hit
GTGCGATCGTCTGGGAGGG170.1935336976320583No Hit
ATGATCACGCCCTGCCCCTTGATGTCGAGCGG160.18214936247723132No Hit
CTCTCCCGTAGGAGTCTGGTCTTGGTCACA160.18214936247723132No Hit
TTGGGCGGATTCGCAGGGCAAACCCTTGG160.18214936247723132No Hit
AGGGAGAGGTTGCAGACGCGCC150.17076502732240437No Hit
CGCTTGGGCGGATTCGCAGGGCAAACCCTTGG150.17076502732240437No Hit
AAGAGAGTCGTTGGCACAGCA140.15938069216757741No Hit
TTGGGTGGCGGTGCTGTGGGTTTTTCCAGGCT140.15938069216757741No Hit
ATTTTCAAGATCCGTCCTGACTCATTCCCT140.15938069216757741No Hit
TACCCTGTTGGGTGGCGGTGCTGTGGGTTTTTCCA140.15938069216757741No Hit
GATTTTCAAGATCCGTCCTGACTCATTCCCT140.15938069216757741No Hit
CTGTTGGGTGGCGGTGCTGTGGGTTTTTCCAGGCT140.15938069216757741No Hit
CTCGCGGCGCTGCCGGGGCCC140.15938069216757741No Hit
CCGCGGAGCGATCGTCGGTGGCC130.14799635701275046No Hit
CAGAGGATATTTGAGCGGCGC130.14799635701275046No Hit
AGGGAGAGGTTGCAGACGCGC130.14799635701275046No Hit
AGAATGTGGCGTCGGTGA130.14799635701275046No Hit
TCCCGTAGGAGTCTGGTCTTGGTCACAG130.14799635701275046No Hit
CAAGAGAGTCGTTGGCACAG130.14799635701275046No Hit
CAAGAGAGTCGTTGGCACA130.14799635701275046No Hit
AGAGAGTCGTTGGCACAGC130.14799635701275046No Hit
TGGCCACGCATGCCGCTTGGGC130.14799635701275046No Hit
AGAGAGTCGTTGGCACA130.14799635701275046No Hit
CTCGCTGAAGCTCTCGCGGCGCTGCCG130.14799635701275046No Hit
GAGAGTCGTTGGCACAG130.14799635701275046No Hit
TCAAGAGAGTCGTTGGCACA120.1366120218579235No Hit
ATGGACGTTAGCCCGGGGAACCTTGCAATC120.1366120218579235No Hit
ACTCAGGCGACATAGGAACC120.1366120218579235No Hit
GTGCGATCGTCTGGGAGGGG120.1366120218579235No Hit
TGGGTGGCGGTGCTGTGGGTTTTTCCAGGCT120.1366120218579235No Hit
GAAGGCGTTTCAAGAGAGTCGTTGGCACA120.1366120218579235No Hit
CACAGACACCGCGGAGC120.1366120218579235No Hit
TAGCGGCATCAAAACCCTGGCC120.1366120218579235No Hit
GTCGGTGACAGTTGGCGC120.1366120218579235No Hit
ATTTTTAAGATCCGTCCTGACTCATTCCCT120.1366120218579235No Hit
TGGCGGTGCTGTGGGTTTTTCCAGGCT120.1366120218579235No Hit
GCAAGAGGAAGAATGGGC120.1366120218579235No Hit
AGAAGAATAAGAATGTGGCGTCGGTGACA120.1366120218579235No Hit
GAATGTGGCGTCGGTGACA120.1366120218579235No Hit
AGCGGCATCAAAACCCTGGCC110.12522768670309653No Hit
AGTTCCATGATCACGCCCTGC110.12522768670309653No Hit
TCTCGCGGCGCTGCC110.12522768670309653No Hit
AAGAGAGTCGTTGGCACA110.12522768670309653No Hit
GGGTGCGATCGTCTGGGAGGG110.12522768670309653No Hit
ACATGGACGTTAGCCCGGGGA110.12522768670309653No Hit
AAAGGTGCTGTGGCTGGG110.12522768670309653No Hit
TTCCATGATCACGCCCTGCCC110.12522768670309653No Hit
TAGCGGCATCAAAACCCTGGCCGAGATTCTAGTCT110.12522768670309653No Hit
TTGGGCGGATTCGCAGGGCAAGCC110.12522768670309653No Hit
TTCAAGAGAGTCGTTGGCACAG110.12522768670309653No Hit
TCTCCCGTAGGAGTCTGGTCTTGGTCACA110.12522768670309653No Hit
CTCGCTGAAGCTCTCGCGGCGCTGCC110.12522768670309653No Hit
CGAAGGCGTTTCAAGAGAGTCGTTGGCAC110.12522768670309653No Hit
GAGACTTACGGGTGCGATCGTCTGGGAGGGGGA110.12522768670309653No Hit
GGCGTTTCAAGAGAGTCGTTGGCACA100.11384335154826959No Hit
AGAGGATATTTGAGCGGCGCG100.11384335154826959No Hit
TCCCGTAGGAGTCTGGTCTTGGTCACAGCA100.11384335154826959No Hit
GTTTCAAGAGAGTCGTTGGCACA100.11384335154826959No Hit
GGGGAACCTTGCAATCCACGCTCAGACGACA100.11384335154826959No Hit
TTGGGCGGATTCGCAGGGCAAACC100.11384335154826959No Hit
CTGTTGGGTGGCGGTGCT100.11384335154826959No Hit
GATCAGATTCATGGGCTCTTTTACGGTCTC100.11384335154826959No Hit
TTTCAAGAGAGTCGTTGGCACA100.11384335154826959No Hit
GGACACCGTCGTTGGT100.11384335154826959No Hit
TTGGGCGGATTCGCAGGGCAAACCCTTGGTA90.10245901639344263No Hit
TGGCGGTGCTGTGGGTTTTTCCAGGC90.10245901639344263No Hit
CTCGCTGAAGCTCTCGCGGCGCTGCCGG90.10245901639344263No Hit
GCGGTGCTGTGGGTTTTTCCA90.10245901639344263No Hit
CGGGGAACCTTGCAATCCACGCTCAGACGACA90.10245901639344263No Hit
GTGGCGTCGGTGACAGTTGGC90.10245901639344263No Hit
AGAAAAGGAAGGCGCGCAAGAGGAAGAATGGGCC90.10245901639344263No Hit
TGTTGGGTGGCGGTGCTGTGGGTTTTTCCA90.10245901639344263No Hit
CGCTCGCTGAAGCTCTCGCGGC90.10245901639344263No Hit
GGTGCGATCGTCTGGGAGGGG90.10245901639344263No Hit
TGATAAGGATGTTTTC90.10245901639344263No Hit
GTGCGATCGTCTGGGAGGGGG90.10245901639344263No Hit
CTGTTGGGTGGCGGTGCTGTG90.10245901639344263No Hit
ATGGACGTTAGCCCGGGGAACCTTGCAATCC90.10245901639344263No Hit
AAGGCGTTTCAAGAGAGTCGTTGGCACA90.10245901639344263No Hit
CATCGTCCTGCGACGTGGAGTGCATGACA90.10245901639344263No Hit
ATTTTCAAGATCCGCCCTGACTCATTCCCT90.10245901639344263No Hit
CCACGCTCAGACGACA90.10245901639344263No Hit
GGTGGCGGTGCTGTGGGTTTTTCCA90.10245901639344263No Hit
GAGACTTACGGGTGCGATCGTCTGGGAGGGG90.10245901639344263No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)