FastQCFastQC Report
Sun 15 May 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.Sobemovirus_segment_2.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.Sobemovirus_segment_2.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1160
Sequences flagged as poor quality0
Sequence length15-26
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTATGAACTCCTGCTGCACC625.344827586206897No Hit
AACGACTCTCTTGAAACGCCT484.137931034482759No Hit
CCCATGAATCTGATCACCCGA292.5No Hit
TCTTGAAACGCCTTCGACACC282.413793103448276No Hit
TCAGGATCCTCTATGAACTCC272.327586206896552No Hit
CTATGAACTCCTGCTGCACCA231.9827586206896552No Hit
TCTGATCACCCGACTCCCGTC181.5517241379310345No Hit
CGTGTATAGGAACTCCCCGCC161.3793103448275863No Hit
CTTGAAACGCCTTCGACACCC151.293103448275862No Hit
AAACCCGGCGGACACACTGTC151.293103448275862No Hit
GCACCAGATCTCACCAACGAC131.1206896551724137No Hit
CCGACGATCGCTCCGCGGTGT121.0344827586206897No Hit
ACCAGATCTCACCAACGACGG121.0344827586206897No Hit
CATTCTTCCTCTTGCGCGCCT121.0344827586206897No Hit
CAAACCCGGCGGACACACTGT110.9482758620689655No Hit
CCAGATCTCACCAACGACGGT100.8620689655172413No Hit
GTGTATAGGAACTCCCCGCCC80.6896551724137931No Hit
CGGGAGAGCCTGGAAAAACCC80.6896551724137931No Hit
TCTCGGAACAAAGCAAACGCT70.603448275862069No Hit
GCCCATGAATCTGATCACCCG70.603448275862069No Hit
GGATTGCAAGGTTCCCCGGGC70.603448275862069No Hit
TCCGCGTCGCCGCCGGTGCCT70.603448275862069No Hit
AAAGGGAATGAGTCAGGACGG60.5172413793103449No Hit
TGCCATCGGCAGGCAGAGGGT60.5172413793103449No Hit
TCTGATCACCCGACTCCC60.5172413793103449No Hit
CCTTTTCTTCCGCGTCGCCGC60.5172413793103449No Hit
TGCACCAGATCTCACCAACGAC60.5172413793103449No Hit
CATGAATCTGATCACCCGACT50.43103448275862066No Hit
ATACCGCGCGGAAAAGCTTCG50.43103448275862066No Hit
CTAACCAGTCGATAGCTCCTC50.43103448275862066No Hit
TGACCAAGACCAGACTCCTAC50.43103448275862066No Hit
GTATAGGAACTCCCCGCC50.43103448275862066No Hit
TTCCGCGTCGCCGCCGGTGCC50.43103448275862066No Hit
TATCAGGATCCTCTATGAACT50.43103448275862066No Hit
TCCCTGCACAACACTCACCCG50.43103448275862066No Hit
CTCCCAGACGATCGCACCCGT50.43103448275862066No Hit
ACTAGAATCTCGGCCAGGGTT50.43103448275862066No Hit
CTCTTGAAACGCCTTCGACACCA40.3448275862068966No Hit
CTCTATGAACTCCTGCTGCAC40.3448275862068966No Hit
CAAACCCGGCGGACA40.3448275862068966No Hit
CTCCCAGACGATCGCACC40.3448275862068966No Hit
GATATACCACTGGCGCGTCTG40.3448275862068966No Hit
TCAACAAGCAACAATCCCGCC40.3448275862068966No Hit
ATCGCGGGCCCATTCTTCCTC40.3448275862068966No Hit
GTATAGGAACTCCCCGCCCCG40.3448275862068966No Hit
ATATACCACTGGCGCGTCTGC40.3448275862068966No Hit
GAAAAGAGACCGTAAAAGAGCT40.3448275862068966No Hit
CTCTTGAAACGCCTTCGACACC40.3448275862068966No Hit
TTCCAGCAAACTCCACCACGT30.25862068965517243No Hit
TTGGAGGGCCCCGGCAGCGCCT30.25862068965517243No Hit
TCCATGTTAGAGGCCGAAGAC30.25862068965517243No Hit
CTTGAAACGCCTTCGACACC30.25862068965517243No Hit
AGGGAATGAGTCAGGACGGAT30.25862068965517243No Hit
GGAATGAGTCAGGACGGATCT30.25862068965517243No Hit
ATCCCAGTCAACACCGTAGGC30.25862068965517243No Hit
CCAGACAAAGGGGTAGGCCTC30.25862068965517243No Hit
CAAACCCGGCGGACACACTGTC30.25862068965517243No Hit
TTCCTTTTCTTCCGCGTCGCC30.25862068965517243No Hit
ATAGGAACTCCCCGCC30.25862068965517243No Hit
ATCGTGTATAGGAACTCCCCG30.25862068965517243No Hit
CGCGCCTTCCTTTTCTTCCGC30.25862068965517243No Hit
ATCGCAAGGTTCCCCGGGCTA30.25862068965517243No Hit
TTGGATGCTGTGCCAACGACT30.25862068965517243No Hit
GTTCCTATGTCGCCTGAGTTC30.25862068965517243No Hit
GTAGAATTGGATGCTGTGC30.25862068965517243No Hit
GAAGACATCGCCCTCCACTCC30.25862068965517243No Hit
AGCCCATGAATCTGATCACCC30.25862068965517243No Hit
CCATGAATCTGATCACCCGA30.25862068965517243No Hit
CTATGGACAAAGGCCACTGCT30.25862068965517243No Hit
GCAAGGTTCCCCGGGCTAACG30.25862068965517243No Hit
TAGGAACTCCCCGCCCCGGCG30.25862068965517243No Hit
CGGAGACAGCAGAAGAG30.25862068965517243No Hit
CTATGAACTCCTGCTGCACC30.25862068965517243No Hit
TGTATAGGAACTCCCCGCCCC30.25862068965517243No Hit
ATTCTTCCTCTTGCGCGCCTT30.25862068965517243No Hit
TTGAAACGCCTTCGACACCCC30.25862068965517243No Hit
CAGGACGATGTTTGCAAAGCC30.25862068965517243No Hit
CAAAGCAAACGCTGTTTCCAGG30.25862068965517243No Hit
AAGCAACAATCCCGCCCCGCG30.25862068965517243No Hit
AAACCCGGCGGACACA30.25862068965517243No Hit
TGAACTCCTGCTGCACCAAGG30.25862068965517243No Hit
CCACCGACGATCGCTCCGCGG30.25862068965517243No Hit
TCCGCGTCGCCGCCG30.25862068965517243No Hit
GAGCGTGCTCGAAACCCCGGA30.25862068965517243No Hit
CGTGCCCTCACGCGACACGG30.25862068965517243No Hit
TCCGCGGTGTCTGTG20.1724137931034483No Hit
CTGCACTGTCGTCTGAGCGTG20.1724137931034483No Hit
GCTTGTAGTAGAATTGGATGC20.1724137931034483No Hit
TCCATGTTAGAGGCCG20.1724137931034483No Hit
CTCTATGAACTCCTGCTGCACC20.1724137931034483No Hit
ACAGGAATGGGGGGCGAC20.1724137931034483No Hit
ATCCTCTATGAACTCCTGCTG20.1724137931034483No Hit
GAGAAGACATCGCCCTCCACT20.1724137931034483No Hit
TTGCCATCGGCAGGCAGAGGG20.1724137931034483No Hit
AACGCTGACGAAACGCTGGCT20.1724137931034483No Hit
AGACTAGAATCTCGGCCA20.1724137931034483No Hit
CTCTCTTGAAACGCCTTCGAC20.1724137931034483No Hit
ATGAACTCCTGCTGCACCAAG20.1724137931034483No Hit
CCATCGTGTATAGGAACTCC20.1724137931034483No Hit
AGGAACTCCCCGCCCCGG20.1724137931034483No Hit
CAAACCCGGCGGACACACT20.1724137931034483No Hit
GATAGCTCCTCAACCACCGCG20.1724137931034483No Hit
AACCAGCTCGCAACG20.1724137931034483No Hit
ACTGCACTGTCGTCTGAGCGT20.1724137931034483No Hit
TTCCGCGTCGCCGCCG20.1724137931034483No Hit
CGCGTCTGCAACCTCTCCCTA20.1724137931034483No Hit
CGTCTGAGCGTGGATTGCA20.1724137931034483No Hit
TGTATAGGAACTCCCCGCCC20.1724137931034483No Hit
TGTATAGGAACTCCCCGCC20.1724137931034483No Hit
CATCGTGTATAGGAACTCCCC20.1724137931034483No Hit
CGCGTCTGCAACCTCTCCCTAT20.1724137931034483No Hit
AAATATCCTCTGAAGACTCCT20.1724137931034483No Hit
CTTCCTCTTGCGCGCCTTCCT20.1724137931034483No Hit
ATAGGAACTCCCCGCCCCG20.1724137931034483No Hit
ATTCTTCCTCTTGCGCGCCT20.1724137931034483No Hit
CGTACCTGCACCAGATCTCACC20.1724137931034483No Hit
CAAACCCGGCGGACACACTGTA20.1724137931034483No Hit
TATGGACAAAGGCCACTGCTG20.1724137931034483No Hit
ACGACTCTCTTGAAACGCCT20.1724137931034483No Hit
AATATGGCCCCCCCTCGG20.1724137931034483No Hit
GTCGATAGCTCCTCAACCACC20.1724137931034483No Hit
TTGGAGGGCCCCGGCAGCGCC20.1724137931034483No Hit
ATCAGGATCCTCTATGAACT20.1724137931034483No Hit
TCGAAACCCCGGATCCTACCC20.1724137931034483No Hit
CTCCCGTCAAGCGATTTCGGC20.1724137931034483No Hit
TATGGCCCCCCCTCGGATTC20.1724137931034483No Hit
ACCAGATCTCACCAACGACGGG20.1724137931034483No Hit
AGGCTTGTAGTAGAATTGGAT20.1724137931034483No Hit
GATCGCACCCGTGAGTCTCGG20.1724137931034483No Hit
CCTCTGAAGACTCCTCATCTAA20.1724137931034483No Hit
CCATGAATCTGATCACCCGAC20.1724137931034483No Hit
ATAGGAACTCCCCGCCCCGG20.1724137931034483No Hit
GGAAGAGAAGAGACTAGAATC20.1724137931034483No Hit
TTCACGCGACACGGGGC20.1724137931034483No Hit
TATAGGAACTCCCCGCCCCGG20.1724137931034483No Hit
TTAAGGCGGCCACCGACG20.1724137931034483No Hit
CACCAGATCTCACCAACGAC20.1724137931034483No Hit
GTGTATAGGAACTCCCCGCC20.1724137931034483No Hit
CGAGAGCTTCAGCGAG20.1724137931034483No Hit
TCACGCGACACGGGGCCC20.1724137931034483No Hit
ATGAACTCCTGCTGCACC20.1724137931034483No Hit
AGACTAGAATCTCGGCCAGGG20.1724137931034483No Hit
AGATCTCACCAACGACGGT20.1724137931034483No Hit
CCCATGAATCTGATCACCCG20.1724137931034483No Hit
TCTTCCGCGTCGCCGCCGGTG20.1724137931034483No Hit
TATGAACTCCTGCTGCACCA20.1724137931034483No Hit
CATGTTAGAGGCCGAAGACG20.1724137931034483No Hit
AGGGAATGAGTCAGGACGGATT20.1724137931034483No Hit
CTATGTCGCCTGAGTTCC20.1724137931034483No Hit
CAGCACCGCCACCCAA20.1724137931034483No Hit
TACCACTGGCGCGTCTGCAAC20.1724137931034483No Hit
CAACGACTCTCTTGAAACGCC20.1724137931034483No Hit
CTCACCCGCTCGACATCAAGG20.1724137931034483No Hit
CTTCTTCCGCGTCGCCGCCGG20.1724137931034483No Hit
GCGCGTCTGCAACCTCTCCCT20.1724137931034483No Hit
GACCAGACAAAGGGGTAGGCC20.1724137931034483No Hit
GAACTCCTGCTGCACCAAGGTA20.1724137931034483No Hit
GACTAGAATCTCGGCC20.1724137931034483No Hit
ATCAGGATCCTCTATGAACTCC20.1724137931034483No Hit
TGAGCGTGGATTGCAAGGTTCCC20.1724137931034483No Hit
CATCGCCCTCCACTCCATCGT20.1724137931034483No Hit
CAAGACCAGACTCCTACGGGA20.1724137931034483No Hit
CACCAGATCTCACCAACGACG20.1724137931034483No Hit
TCTGCAACCTCTCCCTAGGAC20.1724137931034483No Hit
TTTCCCCCTCCCAGACG20.1724137931034483No Hit
AGCGCGCCAACTGTCACCGAC20.1724137931034483No Hit
TGAATCTGATCACCCGACTCC20.1724137931034483No Hit
TAACGCTGACGAAACGCTGGC20.1724137931034483No Hit
CGGAGACAGCAGAAGAGG20.1724137931034483No Hit
TTATCAGGATCCTCTATGAAC20.1724137931034483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCACC50.0322.016
Produced by FastQC (version 0.11.5)