FastQCFastQC Report
Sun 15 May 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.Totivirus.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.Totivirus.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4845
Sequences flagged as poor quality0
Sequence length15-35
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGATCGGGCGCCA67813.993808049535602No Hit
AGACCCAAGGACTCC170.3508771929824561No Hit
AAGCCGCTCGTAGATACGGACCGCACCCTGA110.22703818369453044No Hit
AGAGGACCAGGCACTG110.22703818369453044No Hit
AAGCCGCTCGTAGATACGGACCGCACCCTGAGG100.20639834881320948No Hit
TACAAGGCATGCCTATGGACT100.20639834881320948No Hit
CACACGAACTCCTCCTAGCCT80.1651186790505676No Hit
ATCCCTGTAGCCCGTCGACTTCGTAACTTCG80.1651186790505676No Hit
CAAGTCACACGAACTCCTCCT80.1651186790505676No Hit
TCGACTGATCTAGTGGCACCT80.1651186790505676No Hit
TTTAATGTTGTAGATCCGCCT80.1651186790505676No Hit
TTACAAGGCATGCCTATGGAC80.1651186790505676No Hit
TATGGACTGCGATCGCG80.1651186790505676No Hit
TCTACCCCTGCCATCGTTCGC80.1651186790505676No Hit
GATGTATCCCTGTAGCCCGTC80.1651186790505676No Hit
CTGGGCCGTAGGGGT70.14447884416924664No Hit
TCTGGCCCAGCACCATGTTCCGTGACTCGA70.14447884416924664No Hit
ACCCCTGCCATCGTTCGCTTC70.14447884416924664No Hit
TCTGACAGCATGGCCTACTCC70.14447884416924664No Hit
TCGTAGATACGGACCGCACCC60.12383900928792571No Hit
GAGGACCAGGCACTG60.12383900928792571No Hit
CAAGCCGCTCGTAGATACGGACCGCACCCTGAGG60.12383900928792571No Hit
ACCCCTGCCATCGTTCGCTT60.12383900928792571No Hit
TTCGACTGATCTAGTGGCACC60.12383900928792571No Hit
GACGGGCTGGAACAGGA60.12383900928792571No Hit
AAGTTGACATTCCTCTGGCCC60.12383900928792571No Hit
CATCAACACGGTCATTGTTCAACTCGTAAAGATCA60.12383900928792571No Hit
ATGATGTATCCCTGTAGCCC60.12383900928792571No Hit
AAGCCGGAATGGACTCGCGCC60.12383900928792571No Hit
AGGGGACACGTCGAGGCA60.12383900928792571No Hit
GGGACATGTCTTTAATGTTGTAGATCCGCCT60.12383900928792571No Hit
CACAGCTGCGTAGCTCTGCATTGGCCTCC60.12383900928792571No Hit
CGACTTCGTAACTTCGGCGAAGGAACTCAGCCCT60.12383900928792571No Hit
TGCCTATGGACTGCGATCGCG60.12383900928792571No Hit
TACCGGCTTGGGCTCAGC50.10319917440660474No Hit
GCAAGCCGCTCGTAGATACGGACCGCACCCT50.10319917440660474No Hit
AGAAGATGTGAGGACGTGCAGAACTACGT50.10319917440660474No Hit
ATAATGTCGTGGCTCTCAGATATACAGGCCT50.10319917440660474No Hit
CCGGTTTCTGACAGCATGGCC50.10319917440660474No Hit
GAAACCACGACTGGCTGCTC50.10319917440660474No Hit
TTGTAGAGCTCGCACACC50.10319917440660474No Hit
CAAGCCGCTCGTAGATACGGACCGCACCCTGA50.10319917440660474No Hit
CTCTTCACTCGTAGCCACCTC50.10319917440660474No Hit
AACACTCCTTCGCTTC50.10319917440660474No Hit
CGCGCCGTCAGAACTGA50.10319917440660474No Hit
TCCGGAGGAACCAGTACCACCT50.10319917440660474No Hit
GTCGACACCCAGGTCCTCATC50.10319917440660474No Hit
CTCGCGCCGTCAGAACTGA50.10319917440660474No Hit
ATCAACACGGTCATTGTTCAACTCGTAAAGATCA50.10319917440660474No Hit
GAGCATCTGAGGACAAGGA50.10319917440660474No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCACT50.006917397769.529
GTCCCGT50.006917397769.529
GGCGCCA551.0443364E-716.031259
CCAGATC507.587041E-716.031251
GATCGGG551.0443364E-716.031254
CGGGCGC551.0443364E-716.031257
ATCGGGC551.0443364E-716.031255
CAGATCG551.0443364E-716.031252
GGGCGCC551.0443364E-716.031258
AGATCGG551.0443364E-716.031253
TCGGGCG551.0443364E-716.031256
Produced by FastQC (version 0.11.5)