FastQCFastQC Report
Sun 15 May 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.Totivirus.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.Totivirus.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5253
Sequences flagged as poor quality0
Sequence length15-35
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAGATCTTGGCCATGTTGGACTATCCCCTGGGC130.2474776318294308No Hit
ACCCGTGCGGACGTAATGCTC110.20940415000951837No Hit
TCAGCAGGTCCAGGGC110.20940415000951837No Hit
CTTTGATGAGGACCTGGGTG100.19036740909956215No Hit
GAGACCAGTCGACGCTG90.17133066818960593No Hit
TCTGAGGATAGGAGCCTGGCA90.17133066818960593No Hit
TCGCGGGCAGGATTTTCGGCC90.17133066818960593No Hit
CGACGGGCTACAGGGATACATC80.15229392727964972No Hit
TACAGACTGGAGGATGCCCGCGCTTAAGGTGG70.1332571863696935No Hit
ACGACGAGGTCTGGAACGCTGCCTGCGGCAC70.1332571863696935No Hit
ACCCGTGCGGACGTAATGCTCATA70.1332571863696935No Hit
CGAGGTCTGGAACGCTGCCTGCGGCA70.1332571863696935No Hit
GAGGAGATAGACTGTG60.1142204454597373No Hit
CGCCGAAGTTACGAAGTCGAC60.1142204454597373No Hit
CGGGCTACAGGGATACATCATTCGCACTCTAACA60.1142204454597373No Hit
TACGCGATCGCAGTCCATAGG60.1142204454597373No Hit
ATGTTGGACTATCCCCTGGGC60.1142204454597373No Hit
TAGGTACGAGAGCCCCCCTGG60.1142204454597373No Hit
CACCCGTGCGGACGTAATGCTCATA60.1142204454597373No Hit
GGCCATGTATCCTGGATGTCGGAGT60.1142204454597373No Hit
GCGCAGACCCTGTAGACGCGGCCCAGAACCCGAAT60.1142204454597373No Hit
AGGACACCTTGCACATGGCAGGCAGGCTAGGAGGA60.1142204454597373No Hit
CGCAGACCCTGTAGACGCGGCCCAGAACCCGA60.1142204454597373No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)