FastQCFastQC Report
Sun 15 May 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.clean.adapter1.R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.clean.adapter1.R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29596624
Sequences flagged as poor quality0
Sequence length15-35
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCACGGAATTCTTGAACCAAGCGCAAGCGG28961509.785406605834503No Hit
AATCACGGAATTCTTGAACCAAGCGCAAGCGG11404973.8534699092707334No Hit
ATCACGGAATTCTTGAACCAAGCGCAAGCGGG7471342.524389268181398No Hit
ATCACGGAATTCTTGAACCAAGCGCAAGCG6535242.208103194472451No Hit
AATCACGGAATTCTTGAACCAAGCGCAAGCG3636141.2285657985856764No Hit
AATCACGGAATTCTTGAACCAAGCGCAAGCGGG3626931.2254539571810623No Hit
TCCTCGGTAGTATAGTGGTCAGTATCCCCGCCT3570031.2062287915000036No Hit
GCATCGTTGGTTCAGTGGTAGAATGCTCGCCT3289431.1114206809533411No Hit
TCCTCGGTAGTATAGTGGTCAGTATCCC3078411.04012200851016No Hit
ACGGAATTCTTGAACCAAGCGCAAGCGG2999221.0133655784524613No Hit
AATCACGGAATTCTTGAACCAAGCGCAAGC2942040.9940458073866802No Hit
CGGAATTCTTGAACCAAGCGCAAGCGG2814920.9510949627227754No Hit
ATCACGGAATTCTTGAACCAAGCGCAAGC2757460.931680586272272No Hit
CTCCGGGCGGGACCTCGAATCGTC2261790.7642054039676958No Hit
GCATCGTTGGTTCAGTGGTAGAATGCTCGCC2055460.6944913717186123No Hit
CTTCGCGGGCGTCAAACAGTTCGATGTTGGGA1918680.6482766412817894No Hit
CAATCACGGAATTCTTGAACCAAGCGCAAGC1790250.6048831785679339No Hit
CAATCACGGAATTCTTGAACCAAGCGCAAGCGG1192070.4027722891637911No Hit
GTCGAGACGAAGCAGGCGGGA1166790.39423077442886734No Hit
TTCGCGGGCGTCAAACAGTTCGATGTTGGGA1068310.36095670911655325No Hit
CGTCGAGGGAGGATGGCGACGGATCCGGCC1060550.3583347884542507No Hit
TCCTCGGTAGTATAGTGGTCAGTATCCCCGCC975120.32947000982274194No Hit
GTCGAGACGAAGCAGGCGGGAA875140.2956891299494158No Hit
AGGAACGCTTTTATTAGATCAAAACCCATCGGCGC855800.28915460087609995No Hit
GAGGTGTAGAATAAGTGGGAGACGGG813480.27485567272807876No Hit
CGGGGTCGTGGGTTCGAGCCCCACGTTGGGCGCCA771760.26075947040446235No Hit
CAATCACGGAATTCTTGAACCAAGCGCAAGCGGG770990.26049930559647616No Hit
CTCCGGGCGGGACCTCGAATCGTCC758510.25628260844885553No Hit
TCACGGAATTCTTGAACCAAGCGCAAGCGG724270.24471372140281947No Hit
TCCTCGTAGTATAGTGGTCAGTATCCCCGCCT722700.2441832554956268No Hit
ACGTTTCGGGTCACGTACATCCGAGGGTCGGT721290.24370684980827545No Hit
GAGGTGTAGAATAAGTGGGAGACGG711850.2405172968376393No Hit
ATCACGGAATTCTTGAACCAAGCGCAAGCGGGTCG679530.22959713242969873No Hit
CACGGAATTCTTGAACCAAGCGCAAGCGG662800.22394446069254384No Hit
ACGGAATTCTTGAACCAAGCGCAAGCGGG640630.2164537414807851No Hit
ATCACGGAATTCTTGAACCAAGCGCA618500.2089765373239867No Hit
ATCGAGTCGTCGTCGTGTC616460.20828726952101026No Hit
AGTGCTTCGTCGAGGAGTTGTGGTGTTCGAGTGGT588980.19900242676326868No Hit
GCCTCGGCGGCGCGGCGGTGCT575680.19450867098896146No Hit
GTGCTTCGTCGAGGAGTTGTGGTGTTCGAGTGGT549730.1857407790834522No Hit
TCCTCGTAGTATAGTGGTCAGTATCCC535030.1807739963855337No Hit
TTCGCGGGCGTCAAACAGTTCGATGTTGGGAC531210.1794833086368229No Hit
CGGAATTCTTGAACCAAGCGCAAGCGGG512110.1730298698932689No Hit
TCCTCGGTAGTATAGTGGTCAGTATCCCCGCCTG511900.17295891585472722No Hit
AGGAACGCTTTTATTAGATCAAAACCCATCGGC489410.1653600762032859No Hit
CAATCACGGAATTCTTGAACCAAGCGCAAGCG489050.165238440708643No Hit
GCAGATCGGCCCGCACTCCCGGGGTGCCTCGTTC488930.16519789554376202No Hit
CGTTTCGGGTCACGTACATCCGAGGGTCGGT467610.15799437124991011No Hit
AATCACGGAATTCTTGAACCAAGCGCA456470.1542304284434603No Hit
GCATCGTTGGTTCAGTGGTAGAATGCTC452380.15284851407376732No Hit
CTTCGTCGAGGAGTTGTGGTGTTCGAGTGGT449670.15193286910020548No Hit
CTCGGCGGCGCGGCGGTGCTCGCGGGTCGCGTC422750.14283723711190846No Hit
ATCACGGAATTCTTGAACCAAGCGCAAGCGGGT404290.1366000392477196No Hit
GAACGCTTTTATTAGATCAAAACCCATCGGC393380.13291380800729166No Hit
TCGAGTCGTCGTCGTGTC393020.13279217251264874No Hit
CTCGGTAGTATAGTGGTCAGTATCCC382260.12915662272832198No Hit
CGGCGGCGCGGCGGTGCTCGCGGGTCGCGTC382060.12908904745352037No Hit
ACGACCTCGGGCTGTACGGGACTACCCGG377300.127480755913242No Hit
GCCTTCACGGGCGTCGCGTCGCGGTCCG377080.12740642311096023No Hit
TCGCGGGCGTCAAACAGTTCGATGTTGGGA374120.12640630904389635No Hit
ACGTAACGTTGGCGTC359420.12143952634597784No Hit
TCCTCGGTAGTATAGTGGTCAGTATCCCC351030.11860474356805019No Hit
CCTCGGCGGCGCGGCGGTGCTCGCGGGTCGCGTC341870.11550979598213633No Hit
GTGTGCACCGTGGGGC335490.1133541447159649No Hit
AGCAGATCGGCCCGCACTCCCGGGGTGCCTCGTTC333530.11269190702290911No Hit
ACGGAATTCTTGAACCAAGCGCAAGCG326810.11042137778957492No Hit
CACGGAATTCTTGAACCAAGCGCAAGCGGGTCGCC326360.11026933342127129No Hit
CCTCCGGGCGGGACC325410.10994835086596363No Hit
CACGGAATTCTTGAACCAAGCGCAAGCGGG324920.10978279144269967No Hit
GGGGGTATAGCTCAGTGGTAGAGCATTCGACTG321800.10872861715579452No Hit
GCCTCCGGGCGGGACCTC318240.10752577726432583No Hit
AATCACGGAATTCTTGAACCAAGCGCAAGCGGGT316380.10689732720867082No Hit
CCGTGTGCACCGTGGGGC310940.10505927973406698No Hit
GAGGAACGCTTTTATTAGATCAAAACCCATCGGC299470.10118383772419448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCA169150.0302.8050529
GCCTGCC26550.0265.3536429
TCGGCGC166300.0233.9231629
TCGGCTG12500.0222.2938429
ACGCACG60350.0197.9470729
CTAGCTT7400.0186.0962528
CGCCTGC33250.0150.637328
GGGCGCC183950.0149.6060528
CTCGGCT17700.0143.6844828
GGTACCA3450.0133.1784429
TTCGCCT43650.0132.8109628
GTTCCTC19950.0122.8312429
CCGCCTG104950.0119.0468128
GCCTGTC6600.0116.0266629
ATCGGCG231850.0111.6561928
TAGGCGC603.776315E-4109.39657629
TCGCCTG59650.0107.67477427
ATATATA655.1918667E-4100.9814629
TGGCTTT5450.0100.3638329
TCCGACC66550.098.9582829
Produced by FastQC (version 0.11.5)