FastQCFastQC Report
Sun 15 May 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.clean.adapter1.R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.clean.adapter1.R2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9494
Sequences flagged as poor quality0
Sequence length15-35
%GC75

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGG3413.591742152938698No Hit
GGGGGGGGGGGGGGGGGGGGGG2832.980829997893406No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGG2582.7175057931325046No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGG2582.7175057931325046No Hit
GGGGGGGGGGGGGGGGGGGG2482.6121761112281443No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2392.51737939751422No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2322.4436486201811674No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGG2212.3277859700863703No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2192.3067200337054983No Hit
GGGGGGGGGGGGGGGGGGGGG2182.296187065515062No Hit
GGGGGGGGGGGGGGGGGGG2022.127659574468085No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2022.127659574468085No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG1992.0960606698967768No Hit
GGGGGGGGGGGGGGG1972.0749947335159047No Hit
GGGGGGGGGGGGGGGGGGGGGGG1962.064461765325469No Hit
GGGGGGGGGGGGGGGGGGGGGGGG1871.9696650516115441No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1701.7906045923741312No Hit
GGGGGGGGGGGGGGGGGG1671.759005687802823No Hit
GGGGGGGGGGGGGGGG1651.7379397514219508No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1561.643143037708026No Hit
GGGGGGGGGGGGGGGGG1421.4956814830419212No Hit
AGCGGTTCCGCGTCCAGGTAGCGGAA240.25279123657046554No Hit
AGCGGTTCCGCGTCCAGG120.12639561828523277No Hit
CCGAACCGCGTCGACCGCGCCC110.11586265009479672No Hit
AGCGGTTCCGCGTCCAGGTAGCGGAATGGGGA100.10532968190436064No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATG250.002206324634.85581222
GCGGAAT250.003634188631.55368421
AGCGGAA304.3459423E-429.18224320
TAGCGGA305.713845E-427.87946319
GTAGCGG357.6461554E-526.111518
GGTAGCG300.001389590924.01923217
AGGTAGC300.002156414622.30357216
CCAGGTA402.9942963E-521.7127514
CAGGTAG300.002547354421.68402915
TCCAGGT354.9323076E-419.94853613
GTCCAGG408.73647E-518.95158812
CGTCCAG401.0449659E-418.52330411
GCGGTTC454.2880513E-415.4197532
CGGTTCC454.2880513E-415.4197533
TTCCGCG400.00360456914.8690476
GTTCCGC400.00360456914.8690475
GGTTCCG400.00360456914.8690474
TCCGCGT400.00360456914.8690477
AGCGGTT509.5439574E-413.8777771
GCGTCCA450.00634291613.65027410
Produced by FastQC (version 0.11.5)