FastQCFastQC Report
Sun 15 May 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.clean.adapter2.R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.clean.adapter2.R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29596633
Sequences flagged as poor quality0
Sequence length15-35
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCACGGAATTCTTGAACCAAGCGCAAGCGG28961509.785403630203476No Hit
AATCACGGAATTCTTGAACCAAGCGCAAGCGG11404973.853468737474293No Hit
ATCACGGAATTCTTGAACCAAGCGCAAGCGGG7471342.5243885005432882No Hit
ATCACGGAATTCTTGAACCAAGCGCAAGCG6535242.208102523013344No Hit
AATCACGGAATTCTTGAACCAAGCGCAAGCG3636141.2285654249927687No Hit
AATCACGGAATTCTTGAACCAAGCGCAAGCGGG3626931.22545358453443No Hit
TCCTCGGTAGTATAGTGGTCAGTATCCCCGCCT3570031.2062284246995256No Hit
GCATCGTTGGTTCAGTGGTAGAATGCTCGCCT3289431.1114203429829332No Hit
TCCTCGGTAGTATAGTGGTCAGTATCCC3078411.0401216922208685No Hit
ACGGAATTCTTGAACCAAGCGCAAGCGG2999221.0133652702994966No Hit
AATCACGGAATTCTTGAACCAAGCGCAAGC2942040.9940455051086385No Hit
CGGAATTCTTGAACCAAGCGCAAGCGG2814920.9510946735055977No Hit
ATCACGGAATTCTTGAACCAAGCGCAAGC2757460.9316803029587859No Hit
CTCCGGGCGGGACCTCGAATCGTC2261790.7642051715815107No Hit
GCATCGTTGGTTCAGTGGTAGAATGCTCGCC2055460.6944911605316726No Hit
CTTCGCGGGCGTCAAACAGTTCGATGTTGGGA1918680.6482764441482246No Hit
CAATCACGGAATTCTTGAACCAAGCGCAAGC1790250.6048829946298283No Hit
CAATCACGGAATTCTTGAACCAAGCGCAAGCGG1192070.40277216668531185No Hit
GTCGAGACGAAGCAGGCGGGA1166790.3942306545477657No Hit
TTCGCGGGCGTCAAACAGTTCGATGTTGGGA1068310.36095659935371704No Hit
CGTCGAGGGAGGATGGCGACGGATCCGGCC1060550.3583346794887108No Hit
TCCTCGGTAGTATAGTGGTCAGTATCCCCGCC975120.3294699096346534No Hit
GTCGAGACGAAGCAGGCGGGAA875140.2956890400337092No Hit
AGGAACGCTTTTATTAGATCAAAACCCATCGGCGC855800.2891545129474694No Hit
GAGGTGTAGAATAAGTGGGAGACGGG813480.27485558914758984No Hit
CGGGGTCGTGGGTTCGAGCCCCACGTTGGGCGCCA771760.26075939111046853No Hit
CAATCACGGAATTCTTGAACCAAGCGCAAGCGGG770990.2604992263815955No Hit
CTCCGGGCGGGACCTCGAATCGTCC758510.2562825305162246No Hit
TCACGGAATTCTTGAACCAAGCGCAAGCGG724270.24471364698815573No Hit
TCCTCGTAGTATAGTGGTCAGTATCCCCGCCT722700.2441831812422717No Hit
ACGTTTCGGGTCACGTACATCCGAGGGTCGGT721290.2437067756997899No Hit
GAGGTGTAGAATAAGTGGGAGACGG711850.24051722369906064No Hit
ATCACGGAATTCTTGAACCAAGCGCAAGCGGGTCG679530.22959706261181803No Hit
CACGGAATTCTTGAACCAAGCGCAAGCGG662800.22394439259357646No Hit
ACGGAATTCTTGAACCAAGCGCAAGCGGG640630.21645367565966034No Hit
ATCACGGAATTCTTGAACCAAGCGCA618500.20897647377659478No Hit
ATCGAGTCGTCGTCGTGTC616460.20828720618321686No Hit
AGTGCTTCGTCGAGGAGTTGTGGTGTTCGAGTGGT588980.19900236624889053No Hit
GCCTCGGCGGCGCGGCGGTGCT575680.1945086118410834No Hit
GTGCTTCGTCGAGGAGTTGTGGTGTTCGAGTGGT549730.18574072260179056No Hit
TCCTCGTAGTATAGTGGTCAGTATCCC535030.18077394141421424No Hit
TTCGCGGGCGTCAAACAGTTCGATGTTGGGAC531210.17948325405798693No Hit
CGGAATTCTTGAACCAAGCGCAAGCGGG512110.17302981727685038No Hit
TCCTCGGTAGTATAGTGGTCAGTATCCCCGCCTG511900.172958863259885No Hit
AGGAACGCTTTTATTAGATCAAAACCCATCGGC489410.16536002591916452No Hit
CAATCACGGAATTCTTGAACCAAGCGCAAGCG489050.1652383904615096No Hit
GCAGATCGGCCCGCACTCCCGGGGTGCCTCGTTC488930.16519784530895795No Hit
CGTTTCGGGTCACGTACATCCGAGGGTCGGT467610.157994323205616No Hit
AATCACGGAATTCTTGAACCAAGCGCA456470.15423038154373844No Hit
GCATCGTTGGTTCAGTGGTAGAATGCTC452380.15284846759426993No Hit
CTTCGTCGAGGAGTTGTGGTGTTCGAGTGGT449670.1519328228991453No Hit
CTCGGCGGCGCGGCGGTGCTCGCGGGTCGCGTC422750.14283719367672668No Hit
ATCACGGAATTCTTGAACCAAGCGCAAGCGGGT404290.1365999977091989No Hit
GAACGCTTTTATTAGATCAAAACCCATCGGC393380.13291376758971196No Hit
TCGAGTCGTCGTCGTGTC393020.13279213213205707No Hit
CTCGGTAGTATAGTGGTCAGTATCCC382260.1291565834532597No Hit
CGGCGGCGCGGCGGTGCTCGCGGGTCGCGTC382060.12908900819900695No Hit
ACGACCTCGGGCTGTACGGGACTACCCGG377300.12748071714779177No Hit
GCCTTCACGGGCGTCGCGTCGCGGTCCG377080.12740638436811377No Hit
TCGCGGGCGTCAAACAGTTCGATGTTGGGA374120.12640627060517323No Hit
ACGTAACGTTGGCGTC359420.12143948941759693No Hit
TCCTCGGTAGTATAGTGGTCAGTATCCCC351030.11860470750169454No Hit
CCTCGGCGGCGCGGCGGTGCTCGCGGGTCGCGTC341870.11550976085691911No Hit
GTGTGCACCGTGGGGC335490.11335411024625673No Hit
AGCAGATCGGCCCGCACTCCCGGGGTGCCTCGTTC333530.1126918727545799No Hit
ACGGAATTCTTGAACCAAGCGCAAGCG326810.11042134421168787No Hit
CACGGAATTCTTGAACCAAGCGCAAGCGGGTCGCC326360.1102692998896192No Hit
CCTCCGGGCGGGACC325410.1099483174319187No Hit
CACGGAATTCTTGAACCAAGCGCAAGCGGG324920.1097827580589995No Hit
GGGGGTATAGCTCAGTGGTAGAGCATTCGACTG321800.10872858409265676No Hit
GCCTCCGGGCGGGACCTC318240.107525744566958No Hit
AATCACGGAATTCTTGAACCAAGCGCAAGCGGGT316380.10689729470240754No Hit
CCGTGTGCACCGTGGGGC310940.10505924778673303No Hit
GAGGAACGCTTTTATTAGATCAAAACCCATCGGC299470.10118380695533846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCA170550.0315.4420829
GCCTGCC26300.0264.4631329
TCGGCGC167000.0233.2609329
TCGGCTG12550.0210.7073829
ACGCACG57300.0200.111329
CTAGCTT7150.0182.0521728
GGGCGCC186100.0151.9655828
CGCCTGC33600.0145.689928
CTCGGCT16850.0141.9562828
ATATTTA958.1672624E-10140.3473429
TTCGCCT44800.0136.3359528
AAGCAGT7200.0126.5400229
TGGCTTT6050.0124.8820629
GTTCCTC20400.0122.0012929
CCGCCTG104150.0121.0282328
GCCTGTC6550.0111.9564729
AACGAGG23700.0110.6394929
ATCGGCG230050.0109.6823928
TCGCCTG60750.0108.3663427
GCTAGCT9200.0100.9317427
Produced by FastQC (version 0.11.5)