FastQCFastQC Report
Sun 15 May 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.clean.adapter2.R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.clean.adapter2.R2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9497
Sequences flagged as poor quality0
Sequence length15-35
%GC75

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGG3413.5906075602821943No Hit
GGGGGGGGGGGGGGGGGGGGGG2832.979888385806044No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGG2582.7166473623249447No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGG2582.7166473623249447No Hit
GGGGGGGGGGGGGGGGGGGG2482.6113509529325047No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2392.516584184479309No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2322.4428766979046017No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGG2212.327050647572918No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2192.30599136569443No Hit
GGGGGGGGGGGGGGGGGGGGG2182.2954617247551856No Hit
GGGGGGGGGGGGGGGGGGG2022.1269874697272826No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2022.1269874697272826No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG1992.0953985469095504No Hit
GGGGGGGGGGGGGGG1972.0743392650310626No Hit
GGGGGGGGGGGGGGGGGGGGGGG1962.0638096240918187No Hit
GGGGGGGGGGGGGGGGGGGGGGGG1871.9690428556386228No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1701.7900389596714754No Hit
GGGGGGGGGGGGGGGGGG1671.7584500368537432No Hit
GGGGGGGGGGGGGGGG1651.7373907549752554No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1561.6426239865220595No Hit
GGGGGGGGGGGGGGGGG1421.495209013372644No Hit
AGCGGTTCCGCGTCCAGGTAGCGGAA240.2527113825418553No Hit
AGCGGTTCCGCGTCCAGG120.12635569127092766No Hit
CCGAACCGCGTCGACCGCGCCC110.1158260503316837No Hit
AGCGGTTCCGCGTCCAGGTAGCGGAATGGGGA100.10529640939243971No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)