FastQCFastQC Report
Sun 15 May 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.clean.no-adapter.R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.clean.no-adapter.R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29586951
Sequences flagged as poor quality0
Sequence length15-35
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCACGGAATTCTTGAACCAAGCGCAAGCGG28961509.78860579449366No Hit
AATCACGGAATTCTTGAACCAAGCGCAAGCGG11404973.8547297421758664No Hit
ATCACGGAATTCTTGAACCAAGCGCAAGCGGG7471342.525214578548496No Hit
ATCACGGAATTCTTGAACCAAGCGCAAGCG6535242.208825099957072No Hit
AATCACGGAATTCTTGAACCAAGCGCAAGCG3636131.2289640794686818No Hit
AATCACGGAATTCTTGAACCAAGCGCAAGCGGG3626931.2258546005636064No Hit
TCCTCGGTAGTATAGTGGTCAGTATCCCCGCCT3570031.2066231495093902No Hit
GCATCGTTGGTTCAGTGGTAGAATGCTCGCCT3289431.11178404290459No Hit
TCCTCGGTAGTATAGTGGTCAGTATCCC3078411.0404620604536101No Hit
ACGGAATTCTTGAACCAAGCGCAAGCGG2999221.013696882791336No Hit
AATCACGGAATTCTTGAACCAAGCGCAAGC2942040.9943707954226172No Hit
CGGAATTCTTGAACCAAGCGCAAGCGG2814920.9514059086385751No Hit
ATCACGGAATTCTTGAACCAAGCGCAAGC2757460.9319851849553541No Hit
CTCCGGGCGGGACCTCGAATCGTC2261790.7644552492076658No Hit
GCATCGTTGGTTCAGTGGTAGAATGCTCGCC2055460.6947184250245996No Hit
CTTCGCGGGCGTCAAACAGTTCGATGTTGGGA1918680.6484885853902284No Hit
CAATCACGGAATTCTTGAACCAAGCGCAAGC1790250.6050809358490504No Hit
CAATCACGGAATTCTTGAACCAAGCGCAAGCGG1192070.4029039693883969No Hit
GTCGAGACGAAGCAGGCGGGA1166790.3943596621361897No Hit
TTCGCGGGCGTCAAACAGTTCGATGTTGGGA1068310.3610747183783824No Hit
CGTCGAGGGAGGATGGCGACGGATCCGGCC1060550.3584519405193188No Hit
TCCTCGGTAGTATAGTGGTCAGTATCCCCGCC975090.3295675853858683No Hit
GTCGAGACGAAGCAGGCGGGAA875140.2957858009769239No Hit
AGGAACGCTTTTATTAGATCAAAACCCATCGGCGC855800.28924913553951537No Hit
GAGGTGTAGAATAAGTGGGAGACGGG813480.27494553257616844No Hit
CGGGGTCGTGGGTTCGAGCCCCACGTTGGGCGCCA771760.26084472171532647No Hit
CAATCACGGAATTCTTGAACCAAGCGCAAGCGGG770990.26058447185044514No Hit
CTCCGGGCGGGACCTCGAATCGTCC758510.25636639611834283No Hit
TCACGGAATTCTTGAACCAAGCGCAAGCGG724270.24479372680206216No Hit
TCCTCGTAGTATAGTGGTCAGTATCCCCGCCT722700.2442630874671743No Hit
ACGTTTCGGGTCACGTACATCCGAGGGTCGGT721290.24378652602628773No Hit
GAGGTGTAGAATAAGTGGGAGACGG711850.24059593028021034No Hit
ATCACGGAATTCTTGAACCAAGCGCAAGCGGGTCG679520.22966881582357035No Hit
CACGGAATTCTTGAACCAAGCGCAAGCGG662800.22401767590043328No Hit
ACGGAATTCTTGAACCAAGCGCAAGCGGG640630.21652450771287654No Hit
ATCACGGAATTCTTGAACCAAGCGCA617390.20866969360918602No Hit
ATCGAGTCGTCGTCGTGTC616460.2083553658503034No Hit
AGTGCTTCGTCGAGGAGTTGTGGTGTTCGAGTGGT588980.19906748755557815No Hit
GCCTCGGCGGCGCGGCGGTGCT575680.1945722626167191No Hit
GTGCTTCGTCGAGGAGTTGTGGTGTTCGAGTGGT549730.18580150418338137No Hit
TCCTCGTAGTATAGTGGTCAGTATCCC535030.1808330976720109No Hit
TTCGCGGGCGTCAAACAGTTCGATGTTGGGAC531210.17954198795272958No Hit
CGGAATTCTTGAACCAAGCGCAAGCGGG512110.17308643935632298No Hit
TCCTCGGTAGTATAGTGGTCAGTATCCCCGCCTG511900.17301546212044627No Hit
AGGAACGCTTTTATTAGATCAAAACCCATCGGC489410.1654141381448869No Hit
CAATCACGGAATTCTTGAACCAAGCGCAAGCG489050.16529246288338398No Hit
GCAGATCGGCCCGCACTCCCGGGGTGCCTCGTTC488930.165251904462883No Hit
CGTTTCGGGTCACGTACATCCGAGGGTCGGT467610.15804602508720822No Hit
AATCACGGAATTCTTGAACCAAGCGCA455790.1540510206678613No Hit
GCATCGTTGGTTCAGTGGTAGAATGCTC452380.15289848555195837No Hit
CTTCGTCGAGGAGTTGTGGTGTTCGAGTGGT449670.15198254122231114No Hit
CTCGGCGGCGCGGCGGTGCTCGCGGGTCGCGTC422750.14288393555659049No Hit
ATCACGGAATTCTTGAACCAAGCGCAAGCGGGT404290.13664469853618913No Hit
GAACGCTTTTATTAGATCAAAACCCATCGGC393380.1329572621389747No Hit
TCGAGTCGTCGTCGTGTC393020.13283558687747177No Hit
CTCGGTAGTATAGTGGTCAGTATCCC382260.12919884850588356No Hit
CGGCGGCGCGGCGGTGCTCGCGGGTCGCGTC382060.12913125113838192No Hit
ACGACCTCGGGCTGTACGGGACTACCCGG377300.12752243379184292No Hit
GCCTTCACGGGCGTCGCGTCGCGGTCCG377080.1274480766875911No Hit
TCGCGGGCGTCAAACAGTTCGATGTTGGGA374120.12644763564856684No Hit
ACGTAACGTTGGCGTC359420.12147922913719633No Hit
TCCTCGGTAGTATAGTGGTCAGTATCCCC351030.11864351957050255No Hit
CCTCGGCGGCGCGGCGGTGCTCGCGGGTCGCGTC341870.11554756013892745No Hit
GTGTGCACCGTGGGGC335490.11339120411562516No Hit
AGCAGATCGGCCCGCACTCCCGGGGTGCCTCGTTC333530.1127287499141091No Hit
ACGGAATTCTTGAACCAAGCGCAAGCG326810.110457478366054No Hit
CACGGAATTCTTGAACCAAGCGCAAGCGGGTCGCC326360.11030538428917533No Hit
CCTCCGGGCGGGACC325410.10998429679354253No Hit
CACGGAATTCTTGAACCAAGCGCAAGCGGG324920.10981868324316353No Hit
GGGGGTATAGCTCAGTGGTAGAGCATTCGACTG321800.10876416431013793No Hit
GCCTCCGGGCGGGACCTC318240.10756093116860875No Hit
AATCACGGAATTCTTGAACCAAGCGCAAGCGGGT316360.10692551591409334No Hit
CCGTGTGCACCGTGGGGC310940.10509362725479891No Hit
GAGGAACGCTTTTATTAGATCAAAACCCATCGGC299470.1012169182285799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCA172600.0308.1615329
GCCTGCC26700.0252.92429
TCGGCTG11550.0230.4444929
TCGGCGC165850.0223.8823729
ACGCACG61800.0193.6303329
CTAGCTT7500.0173.2614728
GGGCGCC187900.0150.4313728
CGCCTGC31750.0150.418828
CTCGGCT16350.0137.2179928
TTCGCCT44600.0135.2204628
TTGCGTA1152.1827873E-11133.8946229
GTTCCTC20850.0125.54626529
AAGCAGT8700.0123.8909929
TACCTTG2150.0122.773829
CCGCCTG106650.0122.1559328
TGGCTTT5600.0113.9128929
TCGCCTG58200.0107.7394227
ATCGGCG230600.0106.2486928
TCCGACC68400.097.7643229
CCCGCCT572300.095.889427
Produced by FastQC (version 0.11.5)