FastQCFastQC Report
Sun 15 May 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.clean.no-adapter.R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.clean.no-adapter.R2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9475
Sequences flagged as poor quality0
Sequence length15-35
%GC75

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGG3413.5989445910290243No Hit
GGGGGGGGGGGGGGGGGGGGGG2832.9868073878627968No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGG2582.7229551451187337No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGG2582.7229551451187337No Hit
GGGGGGGGGGGGGGGGGGGG2482.6174142480211082No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2392.5224274406332454No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2322.4485488126649075No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGG2212.33245382585752No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2192.311345646437995No Hit
GGGGGGGGGGGGGGGGGGGGG2182.300791556728232No Hit
GGGGGGGGGGGGGGGGGGG2022.131926121372032No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2022.131926121372032No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG1992.1002638522427444No Hit
GGGGGGGGGGGGGGG1972.079155672823219No Hit
GGGGGGGGGGGGGGGGGGGGGGG1962.0686015831134568No Hit
GGGGGGGGGGGGGGGGGGGGGGGG1871.9736147757255937No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1701.7941952506596308No Hit
GGGGGGGGGGGGGGGGGG1671.762532981530343No Hit
GGGGGGGGGGGGGGGG1651.7414248021108178No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1561.6464379947229553No Hit
GGGGGGGGGGGGGGGGG1421.4986807387862797No Hit
AGCGGTTCCGCGTCCAGGTAGCGGAA240.2532981530343008No Hit
AGCGGTTCCGCGTCCAGG120.1266490765171504No Hit
CCGAACCGCGTCGACCGCGCCC110.11609498680738788No Hit
AGCGGTTCCGCGTCCAGGTAGCGGAATGGGGA100.10554089709762532No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)