FastQCFastQC Report
Wed 18 May 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.Ambiguivirus.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.Ambiguivirus.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119
Sequences flagged as poor quality0
Sequence length15-30
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTTGCAGAACTCGTTGGGC43.361344537815126No Hit
TGCGAGATTGTACCTGGGTGACCT43.361344537815126No Hit
TACCACTCGTCACCAGGAACCC32.5210084033613445No Hit
TTCTAGCTCACGCACTGGACT32.5210084033613445No Hit
TGACAGTCCAGAACGTCGGGC21.680672268907563No Hit
AAACGCCACGGCGAAGCTCTCCC21.680672268907563No Hit
TTCGGCCGGCGCTTCCCCACC21.680672268907563No Hit
TGCCCGCCTCCAAAGGCCACCTG21.680672268907563No Hit
TGACAATCCACCGTCGGCCAGG21.680672268907563No Hit
TAAGACCCAAAACGCAAAGTGCC21.680672268907563No Hit
TCGAGCAAAGCGTCCTGGCGAC21.680672268907563No Hit
TTGTAGTCCCCGGACGCCCT21.680672268907563No Hit
CTTCGCGGGCCGCATC21.680672268907563No Hit
AACCAGCTCGCAACG21.680672268907563No Hit
TAGCGACCGAGGTTTGCACCCT21.680672268907563No Hit
TGCAGTACCACTCGTCACCAGGAACC21.680672268907563No Hit
TATCAAAGCAGCACGCTGGGGCC21.680672268907563No Hit
TCGGCCAGGTCCCCCAAGCCTAAGG21.680672268907563No Hit
TAGGCCTGCTGCGAACTGGGGA21.680672268907563No Hit
TGCGCCACGACACCCGACAGGGC21.680672268907563No Hit
TCCGTCCTCAGTCAAAGCCAGA21.680672268907563No Hit
TCGAGACCAGAACACCAAACCAC21.680672268907563No Hit
GCCACGGCGAAGCTCTCCC21.680672268907563No Hit
TTTGCACCCTCGGCCCG21.680672268907563No Hit
TCATCGAGCAAAGCGTCCTGGC21.680672268907563No Hit
TACTCTCCGGACAGCCGCCCG21.680672268907563No Hit
TCAGACCTTTAGCGACAACCC21.680672268907563No Hit
TCGATACAGTTGGCGTGGACCC10.8403361344537815No Hit
TACAGGACGCCTAGCCAGCTC10.8403361344537815No Hit
TTTGCACCCTCGGCCCGCCCTG10.8403361344537815No Hit
TGAAATCCAGTCCACAGCTT10.8403361344537815No Hit
TTTTGGCAACCGTCGACC10.8403361344537815No Hit
TTGTTGCAGAACTCGTTGGGCC10.8403361344537815No Hit
CCGTGTCCACCGCCC10.8403361344537815No Hit
TACAGGACGCCTAGCCAGC10.8403361344537815No Hit
TTCGACCTCCGACGCATCCTGCATC10.8403361344537815No Hit
TGCCCCTGACACAGAGGACCG10.8403361344537815No Hit
TAGGCCTGCTGCGAACTGGGGAG10.8403361344537815No Hit
TTGCTCCCGACCCTAGGCCCG10.8403361344537815No Hit
GAACGCCTGGAAGAAGATTGTTGCAGAACT10.8403361344537815No Hit
TTAGGCGGACCACGGCC10.8403361344537815No Hit
TCATAATCAACATGCACCCCG10.8403361344537815No Hit
TTTCGGCCGGCGCTTCCCC10.8403361344537815No Hit
TTTGGCCGCCATATCGCCTGGC10.8403361344537815No Hit
TGTAAGAGTTCGCAGTGTCAAG10.8403361344537815No Hit
TTCGACCTCCGACGCAGCCTGCATC10.8403361344537815No Hit
TCGGATCCCAACGCACAAGGG10.8403361344537815No Hit
TACAGTTGGCGTGGACCCTCGG10.8403361344537815No Hit
TCCCCGGACGCCCTC10.8403361344537815No Hit
TCCGACGCAGCCTGCATCTAGC10.8403361344537815No Hit
TCGAGACCAGAACACCAAACCACGA10.8403361344537815No Hit
TCATTCTCCTCTTCGACTCAGT10.8403361344537815No Hit
TCAGACCTTAAGCGACAACCCT10.8403361344537815No Hit
TTCCGCACTTTCCGCCGAATGCCT10.8403361344537815No Hit
TGCGCCTCGAACGCCTTACC10.8403361344537815No Hit
TAAGACCCAAAACGCAAAGTGCCCAGAA10.8403361344537815No Hit
TGCCAGTGTTGTAGTCCCCGGAA10.8403361344537815No Hit
TAAGGAAGTAGTCCGACGAGCACA10.8403361344537815No Hit
TAGGCCTGCTGCGAACTGGGG10.8403361344537815No Hit
TAATCGTGACGCCCCAGTTTAAAG10.8403361344537815No Hit
TAAGAGTTCGCAGTGTCAAGG10.8403361344537815No Hit
TTTCGGCCGGCGCTTCCCCACCC10.8403361344537815No Hit
TGTTGCAGAACTCGTTGGGCCC10.8403361344537815No Hit
TACCTTGACCCAATCACGCACCA10.8403361344537815No Hit
TAAGGAAGTAGTCCGACGAGCAC10.8403361344537815No Hit
TGCTCCCGACCCTAGGCCCGCAC10.8403361344537815No Hit
TGACAATCCACCGTCGGCCAGGT10.8403361344537815No Hit
TCACGCACTGGACTCTCCAA10.8403361344537815No Hit
TTGCTCCCGACCCTAGGCCCGC10.8403361344537815No Hit
TAGCTCCATCTTTGGCCGCC10.8403361344537815No Hit
CCGGACCGGTTTTCC10.8403361344537815No Hit
TTCAAGTTTCCCCAAAGCCAGTGC10.8403361344537815No Hit
TAGGCAAACTGACTGGAACTCTGACAAAG10.8403361344537815No Hit
TCCACGCCGTCGTCATTCTC10.8403361344537815No Hit
TTTCGGCCGGCGCTTCCCCACC10.8403361344537815No Hit
CGAGACCAGAACACCAAACCAC10.8403361344537815No Hit
TAAGGAAGTAGTCCGACGAGC10.8403361344537815No Hit
TGACCGGAGATGGCTAGGCAAACTG10.8403361344537815No Hit
TACGCTCACCGGACTTTCGGCC10.8403361344537815No Hit
AAAGGCAGCGTCGTAGACAGCGA10.8403361344537815No Hit
TTCTAGCTCACGCACTGGACTCT10.8403361344537815No Hit
TTGTCACCATCCACAAGGCTGT10.8403361344537815No Hit
TGCCCGAACGAGAACATGGACC10.8403361344537815No Hit
TCATCGAGCAAAGCGTCCTGGCG10.8403361344537815No Hit
TCGCAGTGTCAAGGTAGCTCCAT10.8403361344537815No Hit
TCGCCTAAAGCGCTTAGATCGGA10.8403361344537815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCAC50.032.017
CAAACCA50.032.016
Produced by FastQC (version 0.11.5)