FastQCFastQC Report
Wed 18 May 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.Ourmiavirus.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.Ourmiavirus.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340
Sequences flagged as poor quality0
Sequence length15-30
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAGTTACCCTGCGAGCCTTGC4513.23529411764706No Hit
TTGCAGACTCGTAGTCCCCGGA154.411764705882353No Hit
TGTAGTTACCCTGCGAGCCTTGCC113.2352941176470593No Hit
TGAACAAACGTCTTCCCGGC102.941176470588235No Hit
TCGGCACACTCGAACACGCAGA92.6470588235294117No Hit
TTCTCGGCACACTCGAACACGC82.3529411764705883No Hit
TTGCAGACTCGTAGTCCCCGGAA72.0588235294117645No Hit
TTCCTTTCTCGGCACACTCGA61.7647058823529411No Hit
TCGGACCGCCACAAAGGG51.4705882352941175No Hit
TTCTCGGCACACTCGAACACGCAG41.1764705882352942No Hit
TCACTTGCAGACTCGTAGTCCCCGG41.1764705882352942No Hit
TGTAGTTACCCTGCGAGCCTTG41.1764705882352942No Hit
TCGGGAACTCGGAGATCGGCTC41.1764705882352942No Hit
TGAACAAACGTCTTCCCGGCC41.1764705882352942No Hit
TTTCTCGGCACACTCGAACACGC41.1764705882352942No Hit
TGTAGTTACCCTGCGAGCCTTGCCG41.1764705882352942No Hit
GTAGTTACCCTGCGAGCCTTGC30.8823529411764706No Hit
TCACCCACCAGGTCGGCCCGTGC30.8823529411764706No Hit
TCGGGAACTCGGAGATCGGCTCA30.8823529411764706No Hit
TGTAGTTACCCTGCGAGCCT30.8823529411764706No Hit
TTGCAGACTCGTAGTCCCCG30.8823529411764706No Hit
TTTCTCGGCACACTCGAACA30.8823529411764706No Hit
TTCGAGTAGAACCCCTCCCAATGA30.8823529411764706No Hit
TACTCATTCGCTGTACATACTCAG30.8823529411764706No Hit
TCCGCCACAGAACTGCTTGATCGACG30.8823529411764706No Hit
TTCGAGTAGAACCCCTCCCAATG30.8823529411764706No Hit
TGCCATCGGCGGACTCAACCCGT30.8823529411764706No Hit
TCCCTCTCAACGACGCCGACC20.5882352941176471No Hit
TCACTTGCAGACTCGTAGTCCCCGGA20.5882352941176471No Hit
TCGTTGTGGAAGTAGCGGCC20.5882352941176471No Hit
TCTTCACCTCCTTCGGGATCCACCG20.5882352941176471No Hit
TGCAGACTCGTAGTCCCCGGAA20.5882352941176471No Hit
TAGCCACTTCTGCTTGGACAGGTG20.5882352941176471No Hit
TTTCAGGCCTAAGCCCTCCACT20.5882352941176471No Hit
TCTCCAGTTGCGGTCCCACCC20.5882352941176471No Hit
TAATTGTCGAAGCAAGGAAACCCG20.5882352941176471No Hit
TTGGACAGGTGTGCATAGAGGAGGGA20.5882352941176471No Hit
TTTGTACGAATCTCAGATAGGCACG20.5882352941176471No Hit
TCAAGTCATCGGGAACTCGGA20.5882352941176471No Hit
TTCGGGATCCACCGCGAAGCTGC20.5882352941176471No Hit
TCGCGACTTTCTTGGAGGGGA20.5882352941176471No Hit
TTGCAGACTCGTAGTCCCCGGAAAC20.5882352941176471No Hit
TCACTCAAGTCATCGGGAACTCGA20.5882352941176471No Hit
TACGTCGTTGTGGAAGTAGCGG20.5882352941176471No Hit
TTACCGATCGTTCCAGCAGTCCG20.5882352941176471No Hit
TACCGATCGTTCCAGCAGTCCG20.5882352941176471No Hit
TCCCCGGAAACAAAAACCTCTCCT20.5882352941176471No Hit
TTCAGAGGGGAGGTTCGAGTAGAA20.5882352941176471No Hit
TGCAAAGACACCCCTCAAGA20.5882352941176471No Hit
TCTTCCCGGCCGACAGTTTCAGG20.5882352941176471No Hit
TTCCTTTCTCGGCACACTCG20.5882352941176471No Hit
TGAACAAACGTCTTCCCGGCCGT20.5882352941176471No Hit
TTCCTTTCTCGGCACACTCGAACA20.5882352941176471No Hit
TGAACAAACGTCTTCCCGGCCGA20.5882352941176471No Hit
TGGACAGGTGTGCATAGAGGA20.5882352941176471No Hit
TAGTCGACAACACGCTCTTTCTCCA10.29411764705882354No Hit
TGCTCGGAGTAGTCGACAACACGG10.29411764705882354No Hit
TACCCTGCGAGCCTTGCC10.29411764705882354No Hit
TCATCCTCGAGCGCATCGGACCG10.29411764705882354No Hit
TAGCCACTTCTGCTTGGACAGG10.29411764705882354No Hit
TGCGGTCCCACCCCACC10.29411764705882354No Hit
TAATTGTCGAAGCAAGGAAACCCGA10.29411764705882354No Hit
TAAGCGACTCCAACGCCACCC10.29411764705882354No Hit
TCGGACCGCCACAAAGGGC10.29411764705882354No Hit
TGATTTTGACGGGTAGG10.29411764705882354No Hit
TACCCCGCCCCCGTTCCTCGCGCG10.29411764705882354No Hit
TAGAGGAGGGTATGAAGGGG10.29411764705882354No Hit
TGTAGTTACCCTGCGAGCCTTGT10.29411764705882354No Hit
ACGCACCGGTCGCGACTTTCTTGGAG10.29411764705882354No Hit
TGAGCCCGCAATTGAAAATCTG10.29411764705882354No Hit
TGCATAGAGGAGGGTATGAAGGG10.29411764705882354No Hit
TCGAGTAGAACCCCTCCCAATGA10.29411764705882354No Hit
TAAGCGACTCCAACGCCACCCT10.29411764705882354No Hit
CGTAGTTACCCTGCGAGCCTTGC10.29411764705882354No Hit
ACCCTGCGAGCCTTGCCGTCCC10.29411764705882354No Hit
CACACTGATGCCCAGACCTCTG10.29411764705882354No Hit
TGCCCAGACCTCTGCGCACGCT10.29411764705882354No Hit
TACTCAGCGACCCCCGGCCTCTG10.29411764705882354No Hit
TCGGGATCCACCGCGAAGCTGC10.29411764705882354No Hit
TGATGCCCAGACCTCTGCGC10.29411764705882354No Hit
TTGACAGTTTCAGGC10.29411764705882354No Hit
TTCCTTTGTACGAATCTCAGATAGGCACGA10.29411764705882354No Hit
TGAGACAGCCGGCTGGGG10.29411764705882354No Hit
TTCGAGTAGAATCCCTCCCAATGAA10.29411764705882354No Hit
TGAAAGAACCCCGCGGCCGAAA10.29411764705882354No Hit
TAGCAGAAGGCGAGGTAGTTTTGC10.29411764705882354No Hit
TTTCTCGGCACACTCGAACAC10.29411764705882354No Hit
TCACTTGCAGACTCGTAGTCCCCG10.29411764705882354No Hit
TCGGGAACTCGGAGATCGGCT10.29411764705882354No Hit
TAGCCACTTCTGCTTGGACAG10.29411764705882354No Hit
TTCGAGTAGAACCCCTCCCA10.29411764705882354No Hit
TTGCAGACTCGTAGTCCCC10.29411764705882354No Hit
TCACTTGCAGACTCGTAGTCCCCGA10.29411764705882354No Hit
TGTACATACTCAGCGACCCCCGGA10.29411764705882354No Hit
TTTTCCGAAAAGAGAACAATGAGCC10.29411764705882354No Hit
TGTAGTTACCCAGCGAGCCTTGC10.29411764705882354No Hit
TTGCAGACTCGTAGTCCCCGGAAAA10.29411764705882354No Hit
TCAAGTCATCGGGAACTCGGAG10.29411764705882354No Hit
TACCCCGCCCCCGTTCCTCGCGCGA10.29411764705882354No Hit
TCAGAGGGGAGGTTCGAGTAGAA10.29411764705882354No Hit
TCGGCACACTCGAACACGCAG10.29411764705882354No Hit
TTCAGAGGGGAGGTTCGAGTAGA10.29411764705882354No Hit
TTTCAGGCCTAAGCCCTCCACTC10.29411764705882354No Hit
TGCTCAAAGCCAGCGAACGCC10.29411764705882354No Hit
TACTCAGCGACCCCCGGCC10.29411764705882354No Hit
TCCCCGGAAACAAAAACCTCTCA10.29411764705882354No Hit
TGCTCGGAGTAGTCGACAACACGA10.29411764705882354No Hit
TCGGCACACTCGAACACGCAGAA10.29411764705882354No Hit
TTGAAAATCTGTCCACCGCC10.29411764705882354No Hit
TCACTTGCAGACTCGTAGTCCCC10.29411764705882354No Hit
CCATCGGCGGACTCAACCCGTTTGTAGA10.29411764705882354No Hit
TCACCCACCAGGTCGGCCCGTG10.29411764705882354No Hit
TCCGACCCTCAACACGCCCGG10.29411764705882354No Hit
TGGAAGTAGCGGCCCACTGCGG10.29411764705882354No Hit
TAGAGGAGGGTATGAAGGGGG10.29411764705882354No Hit
TCATCGGGAACTCGGAGATCGG10.29411764705882354No Hit
TAGTCCCCGGAAACAAAAACCTC10.29411764705882354No Hit
TAGTTACCCTGCGAGCCTTGCCGT10.29411764705882354No Hit
TAAGTCGAGTTCAGGGAGA10.29411764705882354No Hit
TAATTGTCGAAGCAAGGAAACCC10.29411764705882354No Hit
TACCGATCGTTCCAGCAGT10.29411764705882354No Hit
TTACCGATCGTTCCAGCAGT10.29411764705882354No Hit
TTCACCTCCTTCGGGATCCACCG10.29411764705882354No Hit
TACTCAGCGACCCCCGGC10.29411764705882354No Hit
TCTCTCCGACCCTCAACACGC10.29411764705882354No Hit
TCTAAGCGACTCCAACGCCACC10.29411764705882354No Hit
TCCTCGAGCGCATCGGACCG10.29411764705882354No Hit
TCAAGTCATCGGGAACTCGGAGA10.29411764705882354No Hit
TTGCGGTCCCACCCCACC10.29411764705882354No Hit
TTCTCGGCACACTCGAACACGA10.29411764705882354No Hit
TTTCTCGGCACACTCGAACACG10.29411764705882354No Hit
TACTCATTCGCTGTACATACTCA10.29411764705882354No Hit
TTCTCGGCACACTCGAACACGCAGA10.29411764705882354No Hit
TCACCCACCAGGTCGGC10.29411764705882354No Hit
TTGCAGACTCGTAGTCCCCGGAAA10.29411764705882354No Hit
TTGTCACTTGCAGACTCGTAGT10.29411764705882354No Hit
TCGGCACACTCGAACACGCAGAAGT10.29411764705882354No Hit
TCCGAAAAGAGAACAATGAGCCCGCAA10.29411764705882354No Hit
TAATTGTCGAAGCAAGGAAACCCGAAG10.29411764705882354No Hit
TGCAGACTCGTAGTCCCCGGAAA10.29411764705882354No Hit
TTGCAGACTCGTAGTCCCCGGAAACAAAA10.29411764705882354No Hit
TAAGTCGAGTTCAGGGAGAAAAAC10.29411764705882354No Hit
TAGGCACGTGAGACAGCCGGC10.29411764705882354No Hit
TGAAAGAACCCCGCGGCCGAAAGC10.29411764705882354No Hit
GCTCGGAGTAGTCGACAACACGA10.29411764705882354No Hit
TTGCAGACTCGTAGTCCCCGG10.29411764705882354No Hit
TACTCATTCGCTGTACATACT10.29411764705882354No Hit
TCGAGTAGAACCCCTCCCAATGAA10.29411764705882354No Hit
TGAAGTTACCCTGCGAGCCTTGC10.29411764705882354No Hit
TTTCCTTTGTACGAATCTCAGAA10.29411764705882354No Hit
GTCCTTTCTCGGCACACTCGAACACG10.29411764705882354No Hit
TCTAAGCGACTCCAACGCCACCC10.29411764705882354No Hit
TAGCCACTTCTGCTTGGACAGGT10.29411764705882354No Hit
TTGTCGAAGCAAGGAAACCCGAAGC10.29411764705882354No Hit
TAAGTCGAGTTCAGGGAGAAAC10.29411764705882354No Hit
TCGGGAACTCGGAGATCGGCTCCA10.29411764705882354No Hit
TTTCAGGCCTAAGCCCTCCACACCG10.29411764705882354No Hit
TTTACCCCGCCCCCGTTCCTCGCGC10.29411764705882354No Hit
TGCCATCGGCGGACTCA10.29411764705882354No Hit
TAGCCACTTCTGCTTGGACAGGC10.29411764705882354No Hit
TTTTGGAGGCAAAGAAGAGGGA10.29411764705882354No Hit
TAAGCGACTCCAACGCCACCCTC10.29411764705882354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGAA50.098.019
CCAATGA50.098.018
Produced by FastQC (version 0.11.5)