FastQCFastQC Report
Wed 18 May 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.Partitivirus_1_segment_1.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.Partitivirus_1_segment_1.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67991
Sequences flagged as poor quality0
Sequence length15-35
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGTCGGGGTAACCCGAAAAGACCCGG39205.7654689591269435No Hit
TTTCGTGGCCCTCGTTTCC25313.722551514170993No Hit
GTTTCGTGGCCCTCG23523.4592813754761664No Hit
TTTCGTGGCCCTCGTTTCCG21203.118059743201306No Hit
GTTTCGTGGCCCTCGTTTCC18712.751834801664926No Hit
GTTTCGTGGCCCTCGTTTCCG15392.2635348796164196No Hit
TGTTTCGTGGCCCTCG13301.9561412539894987No Hit
TTTCGTGGCCCTCGTTTCCGATC13131.9311379447279786No Hit
TGTTTCGTGGCCCTC12201.7943551352384874No Hit
CCTCGTCGGGGTAACCCGAAAAGACCCGGA11971.7605271285905488No Hit
GTTTCGTGGCCCTCGTTTCCGATC11771.7311114706358193No Hit
TGTTTCGTGGCCCTCGTTTCC10781.5855039637599093No Hit
CCTCGTCGGGGTAACCCGAAAAGACCCG9921.4590166345545734No Hit
ATGTTTCGTGGCCCTC9721.429600976599844No Hit
ATGTTTCGTGGCCCTCG8401.2354576340986307No Hit
TTTCGTGGCCCTCGTTTC7791.1457398773367065No Hit
TTTCGTGGCCCTCGT7731.1369151799502875No Hit
TGTTTCGTGGCCCTCGTTTCCG7251.0663176008589372No Hit
TGTTTCGTGGCCCTCGTTTCCGATC6931.0192525481313703No Hit
GTTTCGTGGCCCTCGT6150.9045314821079261No Hit
GTTTCGTGGCCCTCGTTTC5060.7442161462546514No Hit
GTTTCGTGGCCCTCGTTTCCGA4880.7177420540953949No Hit
TGTTTCGTGGCCCTCGT4840.7118589225044492No Hit
CCGGAGAAGCTGAGGGGAGCTC4840.7118589225044492No Hit
TTCGTGGCCCTCGTTTCCG4810.7074465738112397No Hit
TGTTTCGTGGCCCTCGTTTC4530.6662646526746187No Hit
TGTTTCGTGGCCCTCGTTTCCGA4400.6471444750040446No Hit
GTTTCGTGGCCCTCGTTTCCGAT4260.6265535144357341No Hit
TTTCGTGGCCCTCGTTTCCGA4230.6221411657425248No Hit
TTCGTGGCCCTCGTTTCC4200.6177288170493154No Hit
CCTCGTCGGGGTAACCCGAAAAGACCCGGAGA3950.5809592446059038No Hit
CCTCGTCGGGGTAACCCGA3800.5588975011398567No Hit
CCCGGAGAAGCTGAGGGGAGCTC3730.5486020208557015No Hit
AGGCCTCGTCGGGGTAACCC3630.5338941918783369No Hit
TTTCGTGGCCCTCGTTTCCGAT3460.5088908826168169No Hit
CCTCGTCGGGGTAACCCGAAAAGACCC3370.4956538365371887No Hit
CGGAGAAGCTGAGGGGAGCTC3160.464767395684723No Hit
TTTCGTGGCCCTCGTT3120.4588842640937771No Hit
ATGTTTCGTGGCCCT3040.4471180009118854No Hit
GTTTCGTGGCCCTCGTT2900.4265270403435749No Hit
CATGTTTCGTGGCCCTC2680.39416981659337263No Hit
AGGCCTCGTCGGGGTAACCCGA2670.3926990336956362No Hit
ATGTTTCGTGGCCCTCGTTTCCG2630.3868159021046903No Hit
TTTCGTGGCCCTCGTTTCCGATCTTTGCG2600.3824035534114809No Hit
GTTTCGTGGCCCTCGTTTCCGATCTTTGCGA2520.3706372902295892No Hit
CATGTTTCGTGGCCCTCG2500.3676957244341163No Hit
TGTTTCGTGGCCCTCGTTTCCGAT2450.36034180994543397No Hit
ATGTTTCGTGGCCCTCGTTTCC2420.3559294612522246No Hit
CTCGTCGGGGTAACCCGAAAAGACCCGG2340.34416319807033285No Hit
CCGGAGAAGCTGAGGGGAGCT2300.33828006647938696No Hit
TTTCGTGGCCCTCGTTTCCGATCTTTGCGA2280.335338500683914No Hit
TTTCGTGGCCCTCGTTTCCGATCTTTGCGATCTG2260.33239693488844113No Hit
GAAGGCCTCGTCGGG2150.31621832301334No Hit
TCGTGGCCCTCGTTTCCG2080.30592284272918474No Hit
TTCGTGGCCCTCGTTTC2050.30151049403597535No Hit
AAAGACCCGGAGAAGCTGAGGGGAGCTCCGG2010.2956273624450295No Hit
AGGCCTCGTCGGGGTAACCCGAA1970.28974423085408363No Hit
TTTCGTGGCCCTCGTTTCCGATCT1900.27944875056992835No Hit
TTCGTGGCCCTCGTTTCCGATCTTTGCGATCTG1880.2765071847744554No Hit
ATCTGTGTCGCGAAGG1870.27503640187671896No Hit
ATGTTTCGTGGCCCTCGTTTCCGATC1850.27209483608124607No Hit
CCGGAGAAGCTGAGGGGAGCTCC1840.27062405318350957No Hit
ATGTTTCGTGGCCCTCGT1810.2662117044903002No Hit
TCGTGGCCCTCGTTTCC1790.2632701386948273No Hit
TTCGTGGCCCTCGTTTCCGATC1790.2632701386948273No Hit
CGTGGCCCTCGTTTCCG1790.2632701386948273No Hit
CATGTTTCGTGGCCC1720.25297465841067196No Hit
GGAGCTCCGGGGAGA1710.25150387551293557No Hit
TGTTTCGTGGCCCTCGTT1690.24856230971746263No Hit
TTTCGTGGCCCTCGTTTCCGATCTTTGCGATCTGT1680.24709152681972615No Hit
GTTTCGTGGCCCTCGTTTCCGATCTTTGCG1660.2441499610242532No Hit
CCTCGTCGGGGTAACC1660.2441499610242532No Hit
GATCTGTGTCGCGAAGG1660.2441499610242532No Hit
GTTTCGTGGCCCTCGTTT1630.23973761233104385No Hit
GTGGCCCTCGTTTCCG1620.23826682943330738No Hit
AGGGGAGCTCCGGGGAGA1610.2367960465355709No Hit
CGGAGAAGCTGAGGGGAGCT1590.23385448074009796No Hit
CCGCTCTCATGTTTCGTGGCCC1570.23091291494462501No Hit
CCTCGTCGGGGTAACCCGAAAAGACCCGGAGAAGC1560.22944213204688854No Hit
AAGGCCTCGTCGGGGTAACCCGA1520.2235590004559427No Hit
TGTTTCGTGGCCCTCGTTTCCGATCTTTGCGA1500.22061743466046976No Hit
AGGCCTCGTCGGGGTAACCCGAAA1500.22061743466046976No Hit
GAAGGCCTCGTCGGGGTAACCCGAAA1480.21767586886499682No Hit
CGGAGAAGCTGAGGGGAGCTCC1400.2059096056831051No Hit
TGTTTCGTGGCCCTCGTTTCCGATCTTTGCG1390.20443882278536868No Hit
GAGAAGCTGAGGGGAGCTC1340.19708490829668632No Hit
GGAGAAGCTGAGGGGAGCTC1300.19120177670574046No Hit
CCTCGTCGGGGTAACCCGAAAAGACCCGGAG1290.189730993808004No Hit
TCTGTGTCGCGAAGGC1290.189730993808004No Hit
GGGGAGCTCCGGGGAGA1280.18826021091026754No Hit
TCGTGGCCCTCGTTTCCGATC1280.18826021091026754No Hit
CTCGTCGGGGTAACCCGAAAAGACCCGGA1270.18678942801253107No Hit
ATGTTTCGTGGCCCTCGTTTCCGA1260.1853186451147946No Hit
GTTTCGTGGCCCTCGTTTCCGATCT1240.18237707931932168No Hit
ATGTTTCGTGGCCCTCGTTTC1220.17943551352384873No Hit
CGTGGCCCTCGTTTCC1220.17943551352384873No Hit
CATGTTTCGTGGCCCTCGTTTCCG1200.17649394772837582No Hit
TGAGGGGAGCTCCGGGGAGA1180.17355238193290287No Hit
AAAGACCCGGAGAAGCTGA1170.17208159903516643No Hit
GGCCCTCGTTTCCGATCTTTGCG1170.17208159903516643No Hit
CCTCGTCGGGGTAACCCGAAAAGACC1170.17208159903516643No Hit
AAAGACCCGGAGAAGCTGAGGGGAGCTC1150.16914003323969348No Hit
AAGGCCTCGTCGGGGTAACCCGAA1140.167669250341957No Hit
AAGGCCTCGTCGGGGTAACCC1130.16619846744422057No Hit
CTCATGTTTCGTGGCCCTC1130.16619846744422057No Hit
CCTCGTCGGGGTAACCC1130.16619846744422057No Hit
TCTGTGTCGCGAAGG1120.1647276845464841No Hit
CATGTTTCGTGGCCCTCGTTTCC1120.1647276845464841No Hit
TTTCGTGGCCCTCGTTTCCGATCTTT1120.1647276845464841No Hit
GCCTCGTCGGGGTAACCCGAAAAGACCCGG1110.16325690164874762No Hit
CGCGAAGGCCTCGTCGGGGTAACCCGAAA1070.15737377005780176No Hit
TTTCGTGGCCCTCGTTT1050.15443220426232884No Hit
GAAGGCCTCGTCGGGGTAACCCGAA1040.15296142136459237No Hit
TCATGTTTCGTGGCCCTC1040.15296142136459237No Hit
GAAGGCCTCGTCGGGGTAACCCGA1030.1514906384668559No Hit
CGTGGCCCTCGTTTCCGATC980.14413672397817356No Hit
GTGGCCCTCGTTTCCGATC970.14266594108043712No Hit
CGCTCTCATGTTTCGTGGCCC970.14266594108043712No Hit
ATGTTTCGTGGCCCTCGTTTCCGAT960.14119515818270065No Hit
AAGGCCTCGTCGGGGTAACCCGAAA960.14119515818270065No Hit
AGGCCTCGTCGGGGTAACC950.13972437528496418No Hit
AAAAGACCCGGAGAAGCTGAGGGGAGCTCCGG930.13678280948949126No Hit
CCCGGAGAAGCTGAGGGGAGCTCC930.13678280948949126No Hit
ACCGCTCTCATGTTTCGTGGCCCT920.13531202659175479No Hit
TGTTTCGTGGCCCTCGTTT900.13237046079628184No Hit
ATCTGTGTCGCGAAG890.1308996778985454No Hit
AAGACCCGGAGAAGCTGAGGGGAGCTCCGG880.12942889500080892No Hit
AAAGACCCGGAGAAGCTGAGGGGAGCTCCG880.12942889500080892No Hit
AGACCCGGAGAAGCTGAGGGGAGCTC870.12795811210307245No Hit
GAAAAGACCCGGAGAAGCTGAGGGGAGCTC860.12648732920533598No Hit
TCGTGGCCCTCGTTTC850.12501654630759954No Hit
CTCATGTTTCGTGGCCC830.1220749805121266No Hit
CTCGTCGGGGTAACCCGA820.12060419761439013No Hit
GTGGCCCTCGTTTCCGATCTTTGCGATCTG810.11913341471665369No Hit
CTCATGTTTCGTGGCCCTCGTTTCCGATC810.11913341471665369No Hit
GTTTCGTGGCCCTCGTTTCCGATCTTTGCGAT810.11913341471665369No Hit
CGGAGAAGCTGAGGGGAGC790.11619184892118074No Hit
ATCTGTGTCGCGAAGGCCTCGTCGGGGTAACCCGA780.11472106602344427No Hit
AAGACCCGGAGAAGCTGAGGGGAGCTC780.11472106602344427No Hit
ACCGCTCTCATGTTTCGTGGCCC770.11325028312570783No Hit
AAAGACCCGGAGAAGCTG770.11325028312570783No Hit
CTCTCATGTTTCGTGGCCC770.11325028312570783No Hit
GGGGTAACCCGAAAAGACCCGGAGAAGCTGAG760.11177950022797135No Hit
CTGAGGGGAGCTCCGGGGAGA760.11177950022797135No Hit
CATGTTTCGTGGCCCT750.11030871733023488No Hit
CGAAGGCCTCGTCGGGGTAACCCGAAA750.11030871733023488No Hit
CTCGTCGGGGTAACC750.11030871733023488No Hit
TCATGTTTCGTGGCCCTCG750.11030871733023488No Hit
TCATGTTTCGTGGCCC740.10883793443249841No Hit
CGCGAAGGCCTCGTCGGGGTAACCCGAA740.10883793443249841No Hit
CGTCGGGGTAACCCGAAAAGACCCGGAGAAGCTG740.10883793443249841No Hit
CATGTTTCGTGGCCCTCGTTTCCGATC740.10883793443249841No Hit
CCTCGTCGGGGTAACCCGAAAAGACCCGGAGAA730.10736715153476196No Hit
GGGGTAACCCGAAAAGACCCGGAGAAGCTGA730.10736715153476196No Hit
CGCTCTCATGTTTCGTGGCCCTCGTTTCCGATC720.10589636863702549No Hit
GGGTAACCCGAAAAGACCCGGAGAAGCTG700.10295480284155255No Hit
TTCGTGGCCCTCGTTTCCGAT700.10295480284155255No Hit
AGACCCGGAGAAGCTGAGGGGAGCTCCGG680.10001323704607963No Hit
TTCGTGGCCCTCGTT680.10001323704607963No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCACC50.0070943491048.38129
GATCTGT1751.4060788E-8149.768729
CGATCTG1708.731149E-10125.3282728
GCGATCT1652.264278E-881.6920227
TGCGATC1751.1026714E-660.87373428
GACCCGG9350.053.75715623
TTGCGAT2108.5398824E-753.48882327
ACCCGGA5000.052.06486524
AGACCCG10850.046.7274722
AAGACCC11200.043.24571221
CCCGGAG4650.041.64715225
AAAGACC11050.041.0667220
AAAAGAC10350.039.34465419
TTTGCGA2856.8157533E-939.1134826
CCGGAGA6000.037.15780626
GAAAAGA10350.034.85287518
CGAAAAG10150.030.78799617
CCGAAAA10250.026.7624516
CTTTGCG3753.805326E-924.46222325
CCCGAAA10800.021.8513415
Produced by FastQC (version 0.11.5)