FastQCFastQC Report
Wed 18 May 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.Partitivirus_1_segment_1.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.Partitivirus_1_segment_1.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length15-29
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACGGAAGTCGGGTTCAACTT211.11111111111111No Hit
TCCCCGGAGCTCCCCTCA211.11111111111111No Hit
TATCGATCGAAGCCACTCCAG15.555555555555555No Hit
TATCGATCGAAGCCACTCCAGTC15.555555555555555No Hit
TAGGTCATCCAGTCCCATAGATTCTGAAT15.555555555555555No Hit
CCATCTGACTCTCCT15.555555555555555No Hit
CTCGAGCGTTCTTCC15.555555555555555No Hit
TTCGGCAGGTCCTCTAGATCTC15.555555555555555No Hit
TATCGCGGATGCGGCGAGTTCGGC15.555555555555555No Hit
ACCGGAAGAGACCAAGGGTAGAAAC15.555555555555555No Hit
TTGACAAAAGAGTCGAGTAGCTG15.555555555555555No Hit
TCTCCCCGGAGCTCCCCTCA15.555555555555555No Hit
TGCGGCGAGTTCGGCAGGTCCT15.555555555555555No Hit
TCGTGAACATCCCATTGCTGC15.555555555555555No Hit
TCACGGAAGTCGGGTTCAACT15.555555555555555No Hit
TAACGCCATCGTCACCTTGGACTT15.555555555555555No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)