FastQCFastQC Report
Wed 18 May 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.Partitivirus_1_segment_2.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.Partitivirus_1_segment_2.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32
Sequences flagged as poor quality0
Sequence length15-35
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTTTGTCCTCTCACGGCT26.25No Hit
TTCCAACACTGAGTCTGGTCTGA26.25No Hit
TTTGCTGACGACCTGGGCTTTGA26.25No Hit
TGAACGTGTTGGTCCTGCTGAGT26.25No Hit
CACGCGACATCCATGGCGAAG13.125No Hit
CTCGGAAGTATGTCTCGTGTTAC13.125No Hit
TCCAAATGCTCTCGGAAGTATGTCTCGTGTTAC13.125No Hit
CGACACCCGCCGCCC13.125No Hit
TACTATGAACGTGTTGGTC13.125No Hit
CCCATGTGTTCTACT13.125No Hit
TTGCTGACGACATGGGCTTTGA13.125No Hit
AAATGCTCTCGGAAGTATGTCTCGTGTTAC13.125No Hit
TCACTGAGGATTCGAATCAGATCGACCCAGCCCGT13.125No Hit
TCAGCACTGAACGCTTCTGGCACTCCAAATGCT13.125No Hit
TCGGAAGTATGTCTCGTGTT13.125No Hit
AATGCTCTCGGAAGTATGTCTCGTGTTAC13.125No Hit
TCTGATCGACACCCGAATGGCAA13.125No Hit
TCAGCACTGAACGCTTCTGGCACTCCA13.125No Hit
TCTTTCTGTGGTCTCGTTTCTTCT13.125No Hit
TTTCTCGACCCGCCGTGTCTGGC13.125No Hit
CGTGCAGCCGTCGTCATC13.125No Hit
TGCTGACGACCTGGGCTTTGATA13.125No Hit
GAACGTATCAAGCTCCATGGGGA13.125No Hit
TCTCACGGCTATTCCAACACTG13.125No Hit
TTTAGCTACCCGTACGTCGTACA13.125No Hit
TTCCCCGACCCGCGCC13.125No Hit
ACCCAGCCCGTCGTTTTCTCG13.125No Hit
GCTCTCGGAAGTATGTCTCGTGTTAC13.125No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)