FastQCFastQC Report
Wed 18 May 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.Partitivirus_2_segment_1.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.Partitivirus_2_segment_1.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53
Sequences flagged as poor quality0
Sequence length15-35
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGCTCAGACGACGACTGGC23.7735849056603774No Hit
TAGAATGGCAGACTTGGCG23.7735849056603774No Hit
GTATCTACCCCGCTG23.7735849056603774No Hit
TGAAACAAGACGGTCGCGGTTCT23.7735849056603774No Hit
TTGGTTGCAGACTCCCGTTCAAC23.7735849056603774No Hit
TTGGTTGCAGACTCCCGTTCAA23.7735849056603774No Hit
TATCAAGACGCGGTTGGTC23.7735849056603774No Hit
AGGCGTGGTGACGGCATTCGTGA23.7735849056603774No Hit
TTTAGCAGGTGCACGCGGACATC23.7735849056603774No Hit
AAGGCATTCGTGGAC23.7735849056603774No Hit
AAGGATGGTCGGCCT23.7735849056603774No Hit
TTTGGTTGCAGACTCCCGTTCAACCCGGCAGTTC11.8867924528301887No Hit
TGACAAGGATGGTCGGCCTA11.8867924528301887No Hit
TTATCAGACTTGTTACTCC11.8867924528301887No Hit
CCCGCTCCTACTCGGCAAGAGTG11.8867924528301887No Hit
TCTGGATCTACCCCGCTGAGATG11.8867924528301887No Hit
TTTGGTTGCAGACTCCCGTTCAAC11.8867924528301887No Hit
TTGGTGGACTCAACTCATCGACT11.8867924528301887No Hit
TATGACAAGGATGGTCGGCCTATGGCTAGG11.8867924528301887No Hit
TCTGGATCTACCCCGCTGAGA11.8867924528301887No Hit
TACTCGGCAAGAGTGCGCAGCGACTCTACACAGA11.8867924528301887No Hit
TTGAAGGACAGTACGCACCCATG11.8867924528301887No Hit
TATAAGGCAGCACGCTGGTTAGCC11.8867924528301887No Hit
GTGGACTCAACTCATCGACTCCGTGGTTAACCATA11.8867924528301887No Hit
AGATTGAACCTCTTGATTGGCACA11.8867924528301887No Hit
TTTTCGAAAGACCAACGCCGATG11.8867924528301887No Hit
TATGCGAACGGTCATCAAGTACG11.8867924528301887No Hit
TTAGCCCACCGTATGAAACAAGACG11.8867924528301887No Hit
CCACGCTCAGACGAC11.8867924528301887No Hit
TGTTCAAGTTCGCTGGAAAAACTACCCGA11.8867924528301887No Hit
TATGGCTCCAAGGCCCCGAGAG11.8867924528301887No Hit
TTGAACCTCTTGATTGGCACGAAGTCTC11.8867924528301887No Hit
TAAACACTCCGATACTAATGGC11.8867924528301887No Hit
TGAAACAAGACGGTCGCGGTTC11.8867924528301887No Hit
TACTAATGCCTGACGGACGCAT11.8867924528301887No Hit
TCAGACTTGTTACTCCTGTCC11.8867924528301887No Hit
TTTGACGACCGAACCCTCGGAGT11.8867924528301887No Hit
ACAAGGATGGTCGGCCTA11.8867924528301887No Hit
TTCGCTGGAAAAACTACCCGGTA11.8867924528301887No Hit
TTTGGTTGCAGACTCCCGTTCAACCCG11.8867924528301887No Hit
TCTGGATCTACCCCGCTGAGAT11.8867924528301887No Hit
TTTGGTTGCAGACTCCCGT11.8867924528301887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG50.017.017
TCAAGTA50.017.015
GTCATCA50.017.011
TATGCGA50.017.01
GAACGGT50.017.06
TGCGAAC50.017.03
GCGAACG50.017.04
GGTCATC50.017.010
ATGCGAA50.017.02
CATCAAG50.017.013
CGAACGG50.017.05
ATCAAGT50.017.014
AACGGTC50.017.07
CGGTCAT50.017.09
TCATCAA50.017.012
ACGGTCA50.017.08
CAAGTAC50.017.016
Produced by FastQC (version 0.11.5)