FastQCFastQC Report
Wed 18 May 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.Partitivirus_2_segment_1.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.Partitivirus_2_segment_1.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132
Sequences flagged as poor quality0
Sequence length16-30
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGCAACCAAATCCTTCAGCGG86.0606060606060606No Hit
TCCTTGAGAACATCGCTCCGCCT53.787878787878788No Hit
TTCGAGTTCTCCTCGAGGGCT43.0303030303030303No Hit
TACGTCACTTGAACTGCCGGGTTGA32.272727272727273No Hit
TACGTCACTTGAACTGCCGGGT32.272727272727273No Hit
TTGAGAACATCGCTCCGCCTAGCC32.272727272727273No Hit
TCGTCGCCTAGGACCTTGAGTTG32.272727272727273No Hit
TCTGAGCGTACTTGATGACCGTA32.272727272727273No Hit
TGTGTGGGGATCTGAAGGCA21.5151515151515151No Hit
TTTCCGGATACAACGCCAACTTGAGC21.5151515151515151No Hit
TTGAGCCAGTCGTCGTCTGAGC21.5151515151515151No Hit
TGCGAAACATGCGTCCGTCAGGCA21.5151515151515151No Hit
TTGATATCATCAGCGGGCGAGA21.5151515151515151No Hit
TACGTCACTTGAACTGCCGGGTT21.5151515151515151No Hit
TTCCGGATACAACGCCAACTTGA21.5151515151515151No Hit
TCCTTGAGAACATCGCTCCGC21.5151515151515151No Hit
TATGGATCAGCCAAGTTGGGACT21.5151515151515151No Hit
TATGTCTGGAGGAGGATTTTCCGG21.5151515151515151No Hit
TTTTCCGGATACAACGCCAACTTGA21.5151515151515151No Hit
TATAGATCTCAGGCATACACTCACC21.5151515151515151No Hit
TTGAACGGGAGTCTGCAACCAAATCC21.5151515151515151No Hit
TCTGAGCGTACTTGATGACCGTT21.5151515151515151No Hit
TATGAAATACCACTTCTGGGCAT10.7575757575757576No Hit
TTGCGGAATTGACATGGATGTATACC10.7575757575757576No Hit
TGAGCCAGTCGTCGTCTGAGCG10.7575757575757576No Hit
TAATCGGAACCGCTCACGCGTC10.7575757575757576No Hit
TGTTGAGCTACTTCCAGATCGAAG10.7575757575757576No Hit
TGTGTGGGGATCTGAAGGCAG10.7575757575757576No Hit
TCATCAGCGGGCGAGAGATGTC10.7575757575757576No Hit
GTGTGGGGATCTGAAGGCAG10.7575757575757576No Hit
TTTTGCCTTTCCTTTCGCGTGACTG10.7575757575757576No Hit
TACTGTCCACGAATGCCGTCACCA10.7575757575757576No Hit
TGCGTACTGTCCTTCAACCACC10.7575757575757576No Hit
TGATCTCCAACCATCGGCGT10.7575757575757576No Hit
TAGGACCTTGAGTTGTCGAACGTCC10.7575757575757576No Hit
TTTTGCCTTTCCTTTCGCGTGAC10.7575757575757576No Hit
TAGGAGCGGGGAATCAGGA10.7575757575757576No Hit
TATGGTTAACCACGGAGTCGATGA10.7575757575757576No Hit
TGCAACCAAATCCTTCAGCGGAA10.7575757575757576No Hit
TTACGTCACTTGAACTGCCGGGTTGA10.7575757575757576No Hit
CTGAGCGTACTTGATGACCGTA10.7575757575757576No Hit
TAGCTTGGCGTTGCGGAATTGACA10.7575757575757576No Hit
TCTGAGCGTACTTGATGACCGAT10.7575757575757576No Hit
TTTCGCGTGACTGGAGCAC10.7575757575757576No Hit
GCCCACGCCAAGTCTG10.7575757575757576No Hit
TCGTCTGAGCGTACTTGATGACC10.7575757575757576No Hit
TAGGCCGACCATCCTTGTCATAGATG10.7575757575757576No Hit
TGTGTGGGGATCTGAAGG10.7575757575757576No Hit
TCCTTGAGAACATCGCTCCGCC10.7575757575757576No Hit
AGACGATGCGTCTTGTTGAGCTACT10.7575757575757576No Hit
TAGGAGCGGGGAATCAGGAAGTTC10.7575757575757576No Hit
TGAAACTTGCGAAACATGCGTCCG10.7575757575757576No Hit
TCATACTGTCCACGAATGCCGT10.7575757575757576No Hit
TTAGTATCGGAGTGTTTATGAAA10.7575757575757576No Hit
TCAGCGGAACACAAAGAAGATG10.7575757575757576No Hit
TGAAGGCAGAATCGTCGCCTAGG10.7575757575757576No Hit
TTTGGACTTTGGTCGGATCGAAAGAACCGC10.7575757575757576No Hit
TGAGAACATCGCTCCGCCTAGC10.7575757575757576No Hit
TCGAAAAACTCGCAAAACGTCCT10.7575757575757576No Hit
TACTGTCCACGAATGCCGTCACCACG10.7575757575757576No Hit
TGAGCCAGTCGTCGTCTGAGCGT10.7575757575757576No Hit
TTCTGTGTAGAGTCGCTGCGC10.7575757575757576No Hit
TCGGAACCGCTCACGCGTCTTGGC10.7575757575757576No Hit
TTTCGAAAACCAGCCGTGGTCAGGAC10.7575757575757576No Hit
TGAGAACATCGCTCCGCCTAGCC10.7575757575757576No Hit
TCTGAAGGCAGAATCGTCGCC10.7575757575757576No Hit
TAGATGATCTCCAACCATCGGC10.7575757575757576No Hit
TCAAACATCCCGGGAGGTCTGTAGCC10.7575757575757576No Hit
TTCTCCTCGAGGGCTCACCGAG10.7575757575757576No Hit
TCAGGCATTAGTATCGGAGTGTT10.7575757575757576No Hit
TACTTGATGACCGTTCGCATACC10.7575757575757576No Hit
TTTTCCGGATACAACGCCAACTTGAG10.7575757575757576No Hit
TGAGCCAGTCGTCGTCTGAGC10.7575757575757576No Hit
TCTTGTTTCATACGGTGGG10.7575757575757576No Hit
GAGCCAGTCGTCGTCTGAGC10.7575757575757576No Hit
TTACGTCACTTGAACTGCCGGGT10.7575757575757576No Hit
TTCCGGATACAACGCCAACTTGAGC10.7575757575757576No Hit
TGTATGGTAATCGGAACCGCTCACGC10.7575757575757576No Hit
TAGCTGCGCACTCTTGCCGAGT10.7575757575757576No Hit
TGCAACCAAATCCTTCAGCGGA10.7575757575757576No Hit
TGTGTGGGGATCTGAAGGCAGA10.7575757575757576No Hit
TTTCCAGCGAACTTGAACAGTGAATCA10.7575757575757576No Hit
TTTTCCGGATACAACGCCAACTT10.7575757575757576No Hit
TCTGGAGGAGGATTTTC10.7575757575757576No Hit
TTATAGATCTCAGGCATACACTCACCCT10.7575757575757576No Hit
TTGCGGAATTGACATGGATGTA10.7575757575757576No Hit
TATGGTTAACCACGGAGTCGATG10.7575757575757576No Hit
TACTGTCCACGAATGCCGTCACC10.7575757575757576No Hit
TCGCTGCGCACTCTTGCCGAGTAG10.7575757575757576No Hit
TTCGCGTGACTGGAGCACC10.7575757575757576No Hit
TTGCCTTTCCTTTCGCGTGAC10.7575757575757576No Hit
TCAAACATCCCGGGAGGTCTGTAG10.7575757575757576No Hit
TGTCAAAAGCCGAAAAGTCGAGACC10.7575757575757576No Hit
TTTCCGGATACAACGCCAACTTG10.7575757575757576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTGAG50.036.018
CTTGAGC50.036.019
Produced by FastQC (version 0.11.5)