FastQCFastQC Report
Wed 18 May 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.Partitivirus_2_segment_2.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.Partitivirus_2_segment_2.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172
Sequences flagged as poor quality0
Sequence length15-34
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATTGCTCGAATTCTCACCGGGA158.720930232558139No Hit
TTTCACCCGCGACTGCAAAA95.232558139534884No Hit
TAGAGAAAACTCAGGAGCAGAGA84.651162790697675No Hit
TTTTCACCCGCGACTGCAAAA74.069767441860465No Hit
TTAACCTCAGGAGGCTTCGTCAGA63.488372093023256No Hit
TCACCCGCGACTGCAAAATGGAT63.488372093023256No Hit
TTCGATTGCTCGAATTCTCACCG52.9069767441860463No Hit
TCGATTGCTCGAATTCTCACCGGG52.9069767441860463No Hit
TCGATTGCTCGAATTCTCACCGG52.9069767441860463No Hit
TCTTGGTTACTCCCATAACGGTC42.3255813953488373No Hit
TCTGCGTCGGGGAACCAACGGCG31.744186046511628No Hit
TTCGGTCGGATCAAGGATCTCC31.744186046511628No Hit
TTTCACCCGCGACTGCAAAATG31.744186046511628No Hit
TCGTTCCAGAACAGTCCGAGA31.744186046511628No Hit
TTCCTTAAACTGAGCATCTTCGT21.1627906976744187No Hit
TGGCCAGACCAGAACCACATGAGA21.1627906976744187No Hit
TTCGTCAGATCCCAACGATGT21.1627906976744187No Hit
TAAGGAATTAACCTCAGGAGGCT21.1627906976744187No Hit
TTCGATTGCTCGAATTCTCACCGGGA21.1627906976744187No Hit
TTTCACCCGCGACTGCAAAATGGT21.1627906976744187No Hit
TTGAGGAGTCGGAGCGGAGC21.1627906976744187No Hit
TTCAGGTGCGACGTTTGAGGGT21.1627906976744187No Hit
TCCGAGATCGGCAAGGACGGA21.1627906976744187No Hit
TTCTTCTTGGGTTGCTGCGCG10.5813953488372093No Hit
TAACCTCAGGAGGCTTCGTCAGA10.5813953488372093No Hit
TACTCCCATAACGGTCGTAACGGA10.5813953488372093No Hit
TCGGCAAGGACGGATTGGGGA10.5813953488372093No Hit
TTTTCACCCGCGACTGCAAAAT10.5813953488372093No Hit
TGAGAAAAACTTCTCCATGGTGACT10.5813953488372093No Hit
TGTTCTCACAACAGCAACGGTC10.5813953488372093No Hit
TGGCCGTGACTAGGGTAACCCC10.5813953488372093No Hit
TCGAGACCGGAAATGACATCGTTGATTG10.5813953488372093No Hit
TTCGGAGGTCGTCTGGCTTC10.5813953488372093No Hit
TCACCCGCGACTGCAAAA10.5813953488372093No Hit
TGGATTGAACCACGTTGCAACC10.5813953488372093No Hit
TCACAAGGACGACAGGGTTTT10.5813953488372093No Hit
TAACCGGACTACTTCCAATCA10.5813953488372093No Hit
TTCACCCGCGACTGCAAAA10.5813953488372093No Hit
TAGGGAGCCACATCCGCTTGACAGG10.5813953488372093No Hit
TCCCACGAGAGCGGAGATCTTG10.5813953488372093No Hit
TCTTGGTTACTCCCATAACGGTCGA10.5813953488372093No Hit
TTGCTCGAATTCTCACCGGGATGGC10.5813953488372093No Hit
TCACCCGCGACTGCAAAATGGACA10.5813953488372093No Hit
TAACCGGACTACTTCCAATCACCT10.5813953488372093No Hit
TAATTGAGGATGTTAA10.5813953488372093No Hit
TTGGCCAGACCAGAACCACATGAGA10.5813953488372093No Hit
TCACCGGGATGGCCGTGACTAGGG10.5813953488372093No Hit
TGCCCTTCGGTCGGATCAAGGA10.5813953488372093No Hit
TTCACCCGCGACTGCAAAATGGATTGAACCACGT10.5813953488372093No Hit
TTAACCTCAGGAGGCTTCGTCAG10.5813953488372093No Hit
TCGTTCCAGAACAGTCCGAGAT10.5813953488372093No Hit
TAGGGAGCCACATCCGCTTGAC10.5813953488372093No Hit
TTGGTTACTCCCATAACGGTCGTAA10.5813953488372093No Hit
TATGACTTTCCAGGTCGGTAACCGG10.5813953488372093No Hit
TAGAACTCGTCGAGACCGGAA10.5813953488372093No Hit
CAGATCCCAACGATGTCACAAG10.5813953488372093No Hit
CCCATAACGGTCGTAACGGTCGACA10.5813953488372093No Hit
TAGAAACCGGAAAGAGGGAAGCAACT10.5813953488372093No Hit
TGCAGATTGAGGATGTTAACGC10.5813953488372093No Hit
TGTAGAAACCGGAAAGAGGGAA10.5813953488372093No Hit
TGTGCCGTGTTCGGAGGTCGTCTGGC10.5813953488372093No Hit
TCGATTGCTCGAATTCTCACAG10.5813953488372093No Hit
TGCACTTGGAAGAAAGACATCTGA10.5813953488372093No Hit
TAACCCCATAGTCACCGGTGTAGAAACCA10.5813953488372093No Hit
TTAACCTCAGGAGGCTTCGTCAGAT10.5813953488372093No Hit
TTGCCAGGTTGAGGAGTCGGAGC10.5813953488372093No Hit
TATGCCCTTCGGTCGGATCAAGG10.5813953488372093No Hit
CACCCGCGACTGCAAAA10.5813953488372093No Hit
CGAGACCGGAAATGACATCG10.5813953488372093No Hit
CTACAGTCCGAGATC10.5813953488372093No Hit
TGGTTACTCCCATAACGGTCGTC10.5813953488372093No Hit
TCCAGAACAGTCCGAGATCGGC10.5813953488372093No Hit
TTTGGACTGATCCTTTACACC10.5813953488372093No Hit
TGCCAAAATGTGCCGTGTTCGGA10.5813953488372093No Hit
TTGCCAAAATGTGCCGTGTTCG10.5813953488372093No Hit
TAACAAGGACGACAGGGTTTT10.5813953488372093No Hit
TTTTCACCCGCGACTGCA10.5813953488372093No Hit
TACCGAGGGCGGGAACCGAAAACTC10.5813953488372093No Hit
TAGTCATCAGGCTCGGATGTGC10.5813953488372093No Hit
CTTGGTTACTCCCATAACGGTC10.5813953488372093No Hit
CCCGCGACTGCAAAATG10.5813953488372093No Hit
TCTGCGTCGGGGAACCAACGGC10.5813953488372093No Hit
TTCGATTGCTCGAATTCTCACCGGG10.5813953488372093No Hit
TTGGAGGCAGAGTCGGA10.5813953488372093No Hit
TTTTCACCCGCGACTGCAAAATA10.5813953488372093No Hit
TGTAGAAACCGGAAAGAGGGAAGCA10.5813953488372093No Hit
TACCGAGGGCGGGAACCGAAAACT10.5813953488372093No Hit
TTTCCGAGACCAGGGTTCAACTT10.5813953488372093No Hit
TTGCTCGAATTCTCACCGGGA10.5813953488372093No Hit
TTCGGGACAAAGTTGAAATTCAGGTG10.5813953488372093No Hit
TGGCCAGACCAGAACCACATGAGAAAAAA10.5813953488372093No Hit
TTTTCACCCGCGACTGCAAAATG10.5813953488372093No Hit
TTCGTCAGATCCCAACGATGTC10.5813953488372093No Hit
TTGAGGAGTCGGAGCGGAGCGA10.5813953488372093No Hit
TGCAGATTGAGGATGTTAACGCT10.5813953488372093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCAGA50.051.99999618
Produced by FastQC (version 0.11.5)