FastQCFastQC Report
Wed 18 May 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.Partitivirus_3_segment_1.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.Partitivirus_3_segment_1.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36
Sequences flagged as poor quality0
Sequence length15-35
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGATTGATCGCCTGGCCCC38.333333333333332No Hit
AGCCTTCGTCAATCACCC25.555555555555555No Hit
AGCAGGCTATGGATATCTCGG25.555555555555555No Hit
CACATGTCTGAGCCTCTCCCT25.555555555555555No Hit
ATTGACAGTTTGATTAATC25.555555555555555No Hit
TACAGATCCATTCGTCCATGAACTTCTCAGAGAA12.7777777777777777No Hit
CCAGGACACTGTTTACGAACAACCC12.7777777777777777No Hit
ACATGTCTGAGCCTCTCCCT12.7777777777777777No Hit
GATTGATCGCCTGGC12.7777777777777777No Hit
ACGCCCGATGGACATCGACTCACCCT12.7777777777777777No Hit
TTTCTCTTGGCCTTGTATTTG12.7777777777777777No Hit
ATCCATTCGTCCATGAACTTCTCAGAGAACGGCT12.7777777777777777No Hit
CTCCATCCTCAGTAGCAGGCTATGGATATCTCGGC12.7777777777777777No Hit
CTCACCCTTTCCTAAA12.7777777777777777No Hit
TACAAAATACTCCTTAACAGGTTCACTGGATCG12.7777777777777777No Hit
AAGGATTGATCGCCTGGCCCCTC12.7777777777777777No Hit
TGTCTAATAGGTGCGTCCTATAACAGAATTACC12.7777777777777777No Hit
CAAGATTCGACATGTATGGGG12.7777777777777777No Hit
GAACACGTCGATAATACCGTCGGACTGTATGGACT12.7777777777777777No Hit
AACGCCCGATGGACATCGACTCACCCTTTCCT12.7777777777777777No Hit
ATGGACATCGACTCACCCTTTCC12.7777777777777777No Hit
TCTTAATTGAAGGATTGATCGCC12.7777777777777777No Hit
GGATTGATCGCCTGGCCCCT12.7777777777777777No Hit
ATTCCATCTTCGCTGCAGAAT12.7777777777777777No Hit
TTGGCCTTGTATTTGTTGTAACCCT12.7777777777777777No Hit
GAAGGATTGATCGCCTGGCCC12.7777777777777777No Hit
AGACTCTTCTTTGATCAATTTGGACTGACCCT12.7777777777777777No Hit
AAGCTTCAAGACGCACACAGC12.7777777777777777No Hit
TGAAGGATTGATCGCCTGGCCC12.7777777777777777No Hit
AAGGATTGATCGCCTGGCCC12.7777777777777777No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)