FastQCFastQC Report
Wed 18 May 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.Partitivirus_3_segment_2.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.Partitivirus_3_segment_2.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66
Sequences flagged as poor quality0
Sequence length16-35
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGAACTTCTTTACCCACCGATGCC57.575757575757576No Hit
AACCCACAGAACTTCTTTACCCACCGATGCC46.0606060606060606No Hit
CCCACAGAACTTCTTTACCCACCGATGCC34.545454545454546No Hit
CACAGAACTTCTTTACCCACCGATGCC34.545454545454546No Hit
AACCGATCTTGTCGAACC23.0303030303030303No Hit
ACCCACAGAACTTCTTTACCCACCGATG23.0303030303030303No Hit
TAACCCACAGAACTTCTTTACCCACCGATGCC23.0303030303030303No Hit
CAGTAACCCACAGAACTTCTTTACCCACCGATGCC23.0303030303030303No Hit
AACGCGTGGTCTTATGAGGAT23.0303030303030303No Hit
GCCGGGATGTCTACGACTGATGAAGCCATGGAGA23.0303030303030303No Hit
CGGATCCTACAAAACAGC23.0303030303030303No Hit
CGGACCAGTGGCCCAGCAAACCCGCACACCCGT23.0303030303030303No Hit
CTCAGAATCTGCAAACTTTGGTGTCGTACACA23.0303030303030303No Hit
ACAGAACTTCTTTACCCACCGATGC23.0303030303030303No Hit
ACCCACAGAACTTCTTTACCCACCGATGCC23.0303030303030303No Hit
CAGAACTTCTTTACCCACCGATGCC23.0303030303030303No Hit
GTAACCCACAGAACTTCTTTACCCACCGATGCC11.5151515151515151No Hit
CAGAACTTCTTTACCCACC11.5151515151515151No Hit
GATATCACCCATACGAACACCGATGTATACG11.5151515151515151No Hit
TCGCACGAGACTTCAAGCTC11.5151515151515151No Hit
TAACCCACAGAACTTCTTTACCCACCGATGCA11.5151515151515151No Hit
CCACAGAACTTCTTTACCCACCGATGCC11.5151515151515151No Hit
AATTGCAGCACGTGGTTTCTCTGGAAGTATTGAAC11.5151515151515151No Hit
CAACCGATCTTGTCGAACCCGCT11.5151515151515151No Hit
ACCCACAGAACTTCTTTACCCACCGA11.5151515151515151No Hit
CTTGGTTAATGGCGTAGGAGC11.5151515151515151No Hit
AGTAACCCACAGAACTTCTTTACCCACCGATGCC11.5151515151515151No Hit
AGTAACCCACAGAACTTCTTTACCCACCGATG11.5151515151515151No Hit
CAGTAACCCACAGAACTTCTTTACCCACCGATGC11.5151515151515151No Hit
ATATGTTATGAAGTCGAGGAT11.5151515151515151No Hit
ACCAGAGGCTGCACCA11.5151515151515151No Hit
ACGTGGTTTCTCTGGAAGT11.5151515151515151No Hit
CACTATTAGAAACCTCCCGGGCTTGCG11.5151515151515151No Hit
CTGCAATTGCAGCACGTGGTTTCTCTGGAAGTATT11.5151515151515151No Hit
CAGAACTTCTTTACCCACCGATGC11.5151515151515151No Hit
TGTCTGCTGACACTATTAGAAACCTCCCGGGCTT11.5151515151515151No Hit
GTTTATGACTCTGTTCAGCCGGGCGCTCAAT11.5151515151515151No Hit
ACCCACAGAACTTCTTTACCCACCGATGCAT11.5151515151515151No Hit
AACCGATCTTGTCGAACCCGCTGACA11.5151515151515151No Hit
GCGCAACTACAAGGACCGGACC11.5151515151515151No Hit
TAGTATCCTGCATCCCTGAACCAGA11.5151515151515151No Hit
ACAGAAACCCACAGAACTTCTTTACCAACCGATGC11.5151515151515151No Hit
ACAGAACTTCTTTACCCACCGATG11.5151515151515151No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTACC50.025.015
ACAGAAC50.025.06
TTACCCA50.025.017
CACCGAT50.025.022
AACCCAC50.025.01
CCCACCG50.025.020
CCCACAG50.025.03
AGAACTT50.025.08
ACCGATG50.025.023
ACTTCTT50.025.011
AACTTCT50.025.010
CCACCGA50.025.021
GAACTTC50.025.09
TTCTTTA50.025.013
CCACAGA50.025.04
ACCCACC50.025.019
ACCCACA50.025.02
TACCCAC50.025.018
CGATGCC50.025.025
CAGAACT50.025.07
Produced by FastQC (version 0.11.5)