FastQCFastQC Report
Wed 18 May 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.Sobemovirus_segment_1.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.Sobemovirus_segment_1.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230280
Sequences flagged as poor quality0
Sequence length15-35
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTGTAGGCGACGACAATACC2518610.937120027792254No Hit
ATTGAGTGTAGGCGACGACAATACC178327.743616466909849No Hit
AGTGTAGGCGACGACAATACC154426.705749522320653No Hit
TTGAGTGTAGGCGACGACAATACC145196.304933124891436No Hit
ATTGAGTGTAGGCGACGACAATACCAGGAGTCCC120095.214955706096926No Hit
CTGAATTGAGTGTAGGCGACGACAATACC107294.659110647906896No Hit
AATTGAGTGTAGGCGACGACAATACC75393.273840541948932No Hit
AATTGAGTGTAGGCGACGACAATACCAGGAGTCCC69713.027184297377106No Hit
TTGAGTGTAGGCGACGACAATACCAGGAGTCCC68762.985930171964565No Hit
GAATTGAGTGTAGGCGACGACAATACC60932.645909327774883No Hit
CCCGAGCGCGCGTGGCC46512.0197151294076776No Hit
CGAGCGCGCGTGGCC46442.0166753517457012No Hit
GAGTGTAGGCGACGACAATACC42891.86251519888831No Hit
ATTGAGTGTAGGCGACGACAATACCAGGAGTCC38251.6610213652944243No Hit
CCGAGCGCGCGTGGCC36621.5902379711655374No Hit
TGAATTGAGTGTAGGCGACGACAATACC33961.474726420010422No Hit
TTGAGTGTAGGCGACGACAATACCAGGAGTCC25251.0964912280701753No Hit
GTGTAGGCGACGACAATACC24281.0543685947542123No Hit
AATTGAGTGTAGGCGACGACAATACCAGGAGTCC23761.0317873892652423No Hit
TGAGTGTAGGCGACGACAATACCAGGAGTCCC21930.9523189161021366No Hit
ACTGAATTGAGTGTAGGCGACGACAATACC19930.8654681257599445No Hit
ATTGAGTGTAGGCGACGACAATA16430.7134792426611083No Hit
ATTGAGTGTAGGCGACGACAATACCAGGAGTCCCG15980.693937814834115No Hit
TGAGTGTAGGCGACGACAATAC13020.5653986451276707No Hit
TGAGTGTAGGCGACGACAATA12650.5493312489143651No Hit
CTGAATTGAGTGTAGGCGACGACAATACCAGGA12180.52892131318395No Hit
GAATTGAGTGTAGGCGACGACAATACCAGGAGTCC11900.5167622025360431No Hit
GACTGAATTGAGTGTAGGCGACGACAATACC11410.49548375890220603No Hit
ATTGAGTGTAGGCGACGACAATAC10330.4485843321174222No Hit
TGAGTGTAGGCGACGACAATACCAGGAGTCC9240.4012506513809275No Hit
TTGAGTGTAGGCGACGACAATA8950.38865728678130973No Hit
GGAGTCCCGAGCGCGCGTGGCC8470.3678130970991836No Hit
ACAGACTGAATTGAGTGTAGGCGACGACAATACC8200.3560882404029877No Hit
AGACTGAATTGAGTGTAGGCGACGACAATACC8130.3530484627410109No Hit
GTCCCGAGCGCGCGTGGCC7790.33828382838283827No Hit
ATTGAGTGTAGGCGACGACAATACCAGG7720.33524405072086155No Hit
ATTGAGTGTAGGCGACGACAATACCAGGA7710.33480979676915057No Hit
TTGAGTGTAGGCGACGACAATACCAGGAGTCCCG7500.3256904637832204No Hit
CCCGAGCGCGCGTGGC7440.3230849400729547No Hit
AGGAGTCCCGAGCGCGCGTGGCC7200.3126628452318916No Hit
TTGAGTGTAGGCGACGACAATAC7060.3065832899079382No Hit
CCGAGCGCGCGTGGCCC7050.30614903595622717No Hit
CAGGAGTCCCGAGCGCGCGTGGCC6630.28791036998436687No Hit
TCCCGAGCGCGCGTGGCC6400.2779225290950148No Hit
CCGAGCGCGCGTGGC6130.2661976723988188No Hit
CCCGAGCGCGCGTGGCCC5630.2444849748132708No Hit
TGAGTGTAGGCGACGACAATACCAGGAGTCCCG5460.23710265763418445No Hit
TGAGTGTAGGCGACGACAATACCA5060.21973249956574606No Hit
CAGACTGAATTGAGTGTAGGCGACGACAATACC4990.21669272190376931No Hit
CTGAATTGAGTGTAGGCGACGACAATAC4790.20800764286955012No Hit
ATTGAGTGTAGGCGACGACAATACCAGGAGTC4680.20323084940072955No Hit
ATTGAGTGTAGGCGACGACAATACCA4640.2014938335938857No Hit
CTGAATTGAGTGTAGGCGACGACAATACCA4470.19411151641479937No Hit
AATTGAGTGTAGGCGACGACAATACCAGGA4470.19411151641479937No Hit
GAATTGAGTGTAGGCGACGACAATACCAGGA4380.19020323084940075No Hit
AATTGAGTGTAGGCGACGACAATACCAGG4380.19020323084940075No Hit
GTCCCGAGCGCGCGTGGC4370.18976897689768976No Hit
CCAGGAGTCCCGAGCGCGCGTGGCC4350.18890046899426785No Hit
CGAGCGCGCGTGGCCC4350.18890046899426785No Hit
GAATTGAGTGTAGGCGACGACAATACCAGG3990.17326732673267328No Hit
CTGAATTGAGTGTAGGCGACGACAATACCAGG3970.17239881882925134No Hit
AGCGCGCGTGGCCCT3920.17022754907069654No Hit
ATACCAGGAGTCCCGAGCGCGCGTGGCC3780.16414799374674308No Hit
AGCGCGCGTGGCCCTC3620.15719993051936773No Hit
TTGAGTGTAGGCGACGACAATACCA3410.14808059753343755No Hit
TTGAGTGTAGGCGACGACAATACCAGG3400.1476463435817266No Hit
TGTAGGCGACGACAATACC3370.1463435817265937No Hit
TTGAGTGTAGGCGACGACAATACCAGGAGTC3350.1454750738231718No Hit
AGTGTAGGCGACGACAATAC3140.1363557408372416No Hit
GAGTCCCGAGCGCGCGTGGCC3000.13027618551328818No Hit
GAATTGAGTGTAGGCGACGACAATACCAGGAGT2950.12810491575473337No Hit
AATTGAGTGTAGGCGACGACAATACCAGGAGTC2940.12767066180302242No Hit
AATTGAGTGTAGGCGACGACAATACCAGGAGT2930.12723640785131143No Hit
GAGCGCGCGTGGCCC2910.12636789994788952No Hit
GGAGTCCCGAGCGCGCGTGGC2870.1246308841410457No Hit
CAGGAGTCCCGAGCGCGCGTGGC2800.12159110647906896No Hit
TGAGTGTAGGCGACGACAATACCAGG2700.11724856696195936No Hit
TGAATTGAGTGTAGGCGACGACAATACCAGGA2640.11464304325169358No Hit
GTAGGCGACGACAATACC2610.11334028139656072No Hit
TTACGCCTGAACGCCT2590.11247177349313878No Hit
CGACAATACCAGGAGTCCCGAGCGCGCGTGGCC2560.11116901163800591No Hit
AATTGAGTGTAGGCGACGACAATAC2500.10856348792774013No Hit
GAGTGTAGGCGACGACAATACCAGGAGTCCC2490.1081292339760292No Hit
TACCAGGAGTCCCGAGCGCGCGTGGCC2460.1068264721208963No Hit
TTGAGTGTAGGCGACGACAATACCAGGAGT2400.10422094841063052No Hit
GAATTGAGTGTAGGCGACGACAATACCAGGAGTC2390.10378669445891958No Hit
TGAATTGAGTGTAGGCGACGACAATACCAGG2390.10378669445891958No Hit
ATTGAGTGTAGGCGACGACAATACCAGGAGT2380.10335244050720863No Hit
AATTGAGTGTAGGCGACGACAATACCA2380.10335244050720863No Hit
ATCCGTTCTAATCGTAGTGGTGTACAGACTGA2360.10248393260378669No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCCC42750.068.03483629
AGTCCCG13500.043.08873429
GGAGTCC54900.032.5560527
AGGAGTC55250.028.03674726
CAGGAGT55650.025.99685325
CCAGGAG55000.024.05690424
ACCAGGA58350.019.20464523
TACCAGG61100.017.45441222
ATACCAG61350.016.12397621
AATACCA63600.014.40302820
CTGAATT23450.013.6409891
TGAATTG28700.011.1456872
TTACGCC1654.9943992E-89.6631731
TACAGAC3050.0029964099.18122924
TACGCCT1801.9040817E-78.8579082
GAATTGA37800.08.4624663
ATTGAGT99600.08.4143461
GTACAGA3200.0011547088.20862622
CAATACC178350.08.1241819
ACAGACT4001.6657395E-77.875772524
Produced by FastQC (version 0.11.5)