FastQCFastQC Report
Wed 18 May 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.Sobemovirus_segment_2.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.Sobemovirus_segment_2.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1164
Sequences flagged as poor quality0
Sequence length15-26
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTATGAACTCCTGCTGCACC625.326460481099656No Hit
AACGACTCTCTTGAAACGCCT484.123711340206185No Hit
CCCATGAATCTGATCACCCGA292.491408934707904No Hit
TCTTGAAACGCCTTCGACACC282.405498281786942No Hit
TCAGGATCCTCTATGAACTCC272.3195876288659796No Hit
CTATGAACTCCTGCTGCACCA231.9759450171821304No Hit
TCTGATCACCCGACTCCCGTC181.5463917525773196No Hit
CGTGTATAGGAACTCCCCGCC161.3745704467353952No Hit
CTTGAAACGCCTTCGACACCC151.2886597938144329No Hit
AAACCCGGCGGACACACTGTC151.2886597938144329No Hit
GCACCAGATCTCACCAACGAC131.1168384879725086No Hit
CCGACGATCGCTCCGCGGTGT121.0309278350515463No Hit
ACCAGATCTCACCAACGACGG121.0309278350515463No Hit
CATTCTTCCTCTTGCGCGCCT121.0309278350515463No Hit
CAAACCCGGCGGACACACTGT110.9450171821305842No Hit
CCAGATCTCACCAACGACGGT100.859106529209622No Hit
GTGTATAGGAACTCCCCGCCC80.6872852233676976No Hit
CGGGAGAGCCTGGAAAAACCC80.6872852233676976No Hit
TCTCGGAACAAAGCAAACGCT70.6013745704467355No Hit
GCCCATGAATCTGATCACCCG70.6013745704467355No Hit
GGATTGCAAGGTTCCCCGGGC70.6013745704467355No Hit
TCCGCGTCGCCGCCGGTGCCT70.6013745704467355No Hit
AAAGGGAATGAGTCAGGACGG60.5154639175257731No Hit
TGCCATCGGCAGGCAGAGGGT60.5154639175257731No Hit
TCTGATCACCCGACTCCC60.5154639175257731No Hit
CCTTTTCTTCCGCGTCGCCGC60.5154639175257731No Hit
TGCACCAGATCTCACCAACGAC60.5154639175257731No Hit
CATGAATCTGATCACCCGACT50.429553264604811No Hit
ATACCGCGCGGAAAAGCTTCG50.429553264604811No Hit
CTAACCAGTCGATAGCTCCTC50.429553264604811No Hit
TGACCAAGACCAGACTCCTAC50.429553264604811No Hit
GTATAGGAACTCCCCGCC50.429553264604811No Hit
TTCCGCGTCGCCGCCGGTGCC50.429553264604811No Hit
TATCAGGATCCTCTATGAACT50.429553264604811No Hit
TCCCTGCACAACACTCACCCG50.429553264604811No Hit
CTCCCAGACGATCGCACCCGT50.429553264604811No Hit
ACTAGAATCTCGGCCAGGGTT50.429553264604811No Hit
CTCTTGAAACGCCTTCGACACCA40.3436426116838488No Hit
CTCTATGAACTCCTGCTGCAC40.3436426116838488No Hit
CAAACCCGGCGGACA40.3436426116838488No Hit
CTCCCAGACGATCGCACC40.3436426116838488No Hit
GATATACCACTGGCGCGTCTG40.3436426116838488No Hit
TCAACAAGCAACAATCCCGCC40.3436426116838488No Hit
ATCGCGGGCCCATTCTTCCTC40.3436426116838488No Hit
GTATAGGAACTCCCCGCCCCG40.3436426116838488No Hit
ATATACCACTGGCGCGTCTGC40.3436426116838488No Hit
GAAAAGAGACCGTAAAAGAGCT40.3436426116838488No Hit
CTCTTGAAACGCCTTCGACACC40.3436426116838488No Hit
TTCCAGCAAACTCCACCACGT30.25773195876288657No Hit
TTGGAGGGCCCCGGCAGCGCCT30.25773195876288657No Hit
TCCATGTTAGAGGCCGAAGAC30.25773195876288657No Hit
CTTGAAACGCCTTCGACACC30.25773195876288657No Hit
AGGGAATGAGTCAGGACGGAT30.25773195876288657No Hit
GGAATGAGTCAGGACGGATCT30.25773195876288657No Hit
ATCCCAGTCAACACCGTAGGC30.25773195876288657No Hit
CCAGACAAAGGGGTAGGCCTC30.25773195876288657No Hit
CCGACGATCGCTCCGCGG30.25773195876288657No Hit
CAAACCCGGCGGACACACTGTC30.25773195876288657No Hit
TTCCTTTTCTTCCGCGTCGCC30.25773195876288657No Hit
ATAGGAACTCCCCGCC30.25773195876288657No Hit
ATCGTGTATAGGAACTCCCCG30.25773195876288657No Hit
CGCGCCTTCCTTTTCTTCCGC30.25773195876288657No Hit
ATCGCAAGGTTCCCCGGGCTA30.25773195876288657No Hit
TTGGATGCTGTGCCAACGACT30.25773195876288657No Hit
GTTCCTATGTCGCCTGAGTTC30.25773195876288657No Hit
GTAGAATTGGATGCTGTGC30.25773195876288657No Hit
GAAGACATCGCCCTCCACTCC30.25773195876288657No Hit
AGCCCATGAATCTGATCACCC30.25773195876288657No Hit
CCATGAATCTGATCACCCGA30.25773195876288657No Hit
CTATGGACAAAGGCCACTGCT30.25773195876288657No Hit
GCAAGGTTCCCCGGGCTAACG30.25773195876288657No Hit
TAGGAACTCCCCGCCCCGGCG30.25773195876288657No Hit
CGGAGACAGCAGAAGAG30.25773195876288657No Hit
CTATGAACTCCTGCTGCACC30.25773195876288657No Hit
TGTATAGGAACTCCCCGCCCC30.25773195876288657No Hit
ATTCTTCCTCTTGCGCGCCTT30.25773195876288657No Hit
TTGAAACGCCTTCGACACCCC30.25773195876288657No Hit
CAGGACGATGTTTGCAAAGCC30.25773195876288657No Hit
CAAAGCAAACGCTGTTTCCAGG30.25773195876288657No Hit
AAGCAACAATCCCGCCCCGCG30.25773195876288657No Hit
AAACCCGGCGGACACA30.25773195876288657No Hit
TGAACTCCTGCTGCACCAAGG30.25773195876288657No Hit
CCACCGACGATCGCTCCGCGG30.25773195876288657No Hit
TCCGCGTCGCCGCCG30.25773195876288657No Hit
GAGCGTGCTCGAAACCCCGGA30.25773195876288657No Hit
CGTGCCCTCACGCGACACGG30.25773195876288657No Hit
CTGCACTGTCGTCTGAGCGTG20.1718213058419244No Hit
GCTTGTAGTAGAATTGGATGC20.1718213058419244No Hit
TCCATGTTAGAGGCCG20.1718213058419244No Hit
CTCTATGAACTCCTGCTGCACC20.1718213058419244No Hit
ACAGGAATGGGGGGCGAC20.1718213058419244No Hit
ATCCTCTATGAACTCCTGCTG20.1718213058419244No Hit
GAGAAGACATCGCCCTCCACT20.1718213058419244No Hit
TTGCCATCGGCAGGCAGAGGG20.1718213058419244No Hit
AACGCTGACGAAACGCTGGCT20.1718213058419244No Hit
AGACTAGAATCTCGGCCA20.1718213058419244No Hit
CTCTCTTGAAACGCCTTCGAC20.1718213058419244No Hit
ATGAACTCCTGCTGCACCAAG20.1718213058419244No Hit
CCATCGTGTATAGGAACTCC20.1718213058419244No Hit
AGGAACTCCCCGCCCCGG20.1718213058419244No Hit
CAAACCCGGCGGACACACT20.1718213058419244No Hit
GATAGCTCCTCAACCACCGCG20.1718213058419244No Hit
AACCAGCTCGCAACG20.1718213058419244No Hit
ACTGCACTGTCGTCTGAGCGT20.1718213058419244No Hit
TTCCGCGTCGCCGCCG20.1718213058419244No Hit
CGCGTCTGCAACCTCTCCCTA20.1718213058419244No Hit
TGTATAGGAACTCCCCGCCC20.1718213058419244No Hit
TCCGCGGTGTCTGTGAGATCG20.1718213058419244No Hit
TGTATAGGAACTCCCCGCC20.1718213058419244No Hit
CATCGTGTATAGGAACTCCCC20.1718213058419244No Hit
CGCGTCTGCAACCTCTCCCTAT20.1718213058419244No Hit
AAATATCCTCTGAAGACTCCT20.1718213058419244No Hit
CTTCCTCTTGCGCGCCTTCCT20.1718213058419244No Hit
ATAGGAACTCCCCGCCCCG20.1718213058419244No Hit
ATTCTTCCTCTTGCGCGCCT20.1718213058419244No Hit
CGTACCTGCACCAGATCTCACC20.1718213058419244No Hit
CAAACCCGGCGGACACACTGTA20.1718213058419244No Hit
TATGGACAAAGGCCACTGCTG20.1718213058419244No Hit
ACGACTCTCTTGAAACGCCT20.1718213058419244No Hit
AATATGGCCCCCCCTCGG20.1718213058419244No Hit
GTCGATAGCTCCTCAACCACC20.1718213058419244No Hit
CGTCTGAGCGTGGATTGCAAGA20.1718213058419244No Hit
TTGGAGGGCCCCGGCAGCGCC20.1718213058419244No Hit
ATCAGGATCCTCTATGAACT20.1718213058419244No Hit
TCGAAACCCCGGATCCTACCC20.1718213058419244No Hit
CTCCCGTCAAGCGATTTCGGC20.1718213058419244No Hit
TATGGCCCCCCCTCGGATTC20.1718213058419244No Hit
ACCAGATCTCACCAACGACGGG20.1718213058419244No Hit
AGGCTTGTAGTAGAATTGGAT20.1718213058419244No Hit
GATCGCACCCGTGAGTCTCGG20.1718213058419244No Hit
CCTCTGAAGACTCCTCATCTAA20.1718213058419244No Hit
CCATGAATCTGATCACCCGAC20.1718213058419244No Hit
ATAGGAACTCCCCGCCCCGG20.1718213058419244No Hit
GGAAGAGAAGAGACTAGAATC20.1718213058419244No Hit
TTCACGCGACACGGGGC20.1718213058419244No Hit
TATAGGAACTCCCCGCCCCGG20.1718213058419244No Hit
TTAAGGCGGCCACCGACG20.1718213058419244No Hit
CACCAGATCTCACCAACGAC20.1718213058419244No Hit
GTGTATAGGAACTCCCCGCC20.1718213058419244No Hit
CGAGAGCTTCAGCGAG20.1718213058419244No Hit
TCACGCGACACGGGGCCC20.1718213058419244No Hit
ATGAACTCCTGCTGCACC20.1718213058419244No Hit
AGACTAGAATCTCGGCCAGGG20.1718213058419244No Hit
AGATCTCACCAACGACGGT20.1718213058419244No Hit
CCCATGAATCTGATCACCCG20.1718213058419244No Hit
TCTTCCGCGTCGCCGCCGGTG20.1718213058419244No Hit
TATGAACTCCTGCTGCACCA20.1718213058419244No Hit
CATGTTAGAGGCCGAAGACG20.1718213058419244No Hit
AGGGAATGAGTCAGGACGGATT20.1718213058419244No Hit
CTATGTCGCCTGAGTTCC20.1718213058419244No Hit
CAGCACCGCCACCCAA20.1718213058419244No Hit
TACCACTGGCGCGTCTGCAAC20.1718213058419244No Hit
CAACGACTCTCTTGAAACGCC20.1718213058419244No Hit
CTCACCCGCTCGACATCAAGG20.1718213058419244No Hit
CTTCTTCCGCGTCGCCGCCGG20.1718213058419244No Hit
GCGCGTCTGCAACCTCTCCCT20.1718213058419244No Hit
GACCAGACAAAGGGGTAGGCC20.1718213058419244No Hit
GAACTCCTGCTGCACCAAGGTA20.1718213058419244No Hit
GACTAGAATCTCGGCC20.1718213058419244No Hit
ATCAGGATCCTCTATGAACTCC20.1718213058419244No Hit
TGAGCGTGGATTGCAAGGTTCCC20.1718213058419244No Hit
CATCGCCCTCCACTCCATCGT20.1718213058419244No Hit
CAAGACCAGACTCCTACGGGA20.1718213058419244No Hit
CACCAGATCTCACCAACGACG20.1718213058419244No Hit
TCTGCAACCTCTCCCTAGGAC20.1718213058419244No Hit
TTTCCCCCTCCCAGACG20.1718213058419244No Hit
AGCGCGCCAACTGTCACCGAC20.1718213058419244No Hit
TGAATCTGATCACCCGACTCC20.1718213058419244No Hit
TAACGCTGACGAAACGCTGGC20.1718213058419244No Hit
CGGAGACAGCAGAAGAGGAGA20.1718213058419244No Hit
TTATCAGGATCCTCTATGAAC20.1718213058419244No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)