FastQCFastQC Report
Wed 18 May 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.Totivirus.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.Totivirus.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4847
Sequences flagged as poor quality0
Sequence length15-35
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGATCGGGCGCCA67813.98803383536208No Hit
AGACCCAAGGACTCC170.3507324118011141No Hit
AAGCCGCTCGTAGATACGGACCGCACCCTGA110.22694450175366207No Hit
AGAGGACCAGGCACTG110.22694450175366207No Hit
AAGCCGCTCGTAGATACGGACCGCACCCTGAGG100.20631318341242005No Hit
TACAAGGCATGCCTATGGACT100.20631318341242005No Hit
CACACGAACTCCTCCTAGCCT80.16505054672993605No Hit
ATCCCTGTAGCCCGTCGACTTCGTAACTTCG80.16505054672993605No Hit
CAAGTCACACGAACTCCTCCT80.16505054672993605No Hit
TCGACTGATCTAGTGGCACCT80.16505054672993605No Hit
TTTAATGTTGTAGATCCGCCT80.16505054672993605No Hit
TTACAAGGCATGCCTATGGAC80.16505054672993605No Hit
TATGGACTGCGATCGCG80.16505054672993605No Hit
TCTACCCCTGCCATCGTTCGC80.16505054672993605No Hit
GATGTATCCCTGTAGCCCGTC80.16505054672993605No Hit
TCTGGCCCAGCACCATGTTCCGTGACTCGA70.14441922838869403No Hit
ACCCCTGCCATCGTTCGCTTC70.14441922838869403No Hit
TCTGACAGCATGGCCTACTCC70.14441922838869403No Hit
TCGTAGATACGGACCGCACCC60.12378791004745203No Hit
GAGGACCAGGCACTG60.12378791004745203No Hit
CAAGCCGCTCGTAGATACGGACCGCACCCTGAGG60.12378791004745203No Hit
ACCCCTGCCATCGTTCGCTT60.12378791004745203No Hit
TTCGACTGATCTAGTGGCACC60.12378791004745203No Hit
GACGGGCTGGAACAGGA60.12378791004745203No Hit
AAGTTGACATTCCTCTGGCCC60.12378791004745203No Hit
CATCAACACGGTCATTGTTCAACTCGTAAAGATCA60.12378791004745203No Hit
ATGATGTATCCCTGTAGCCC60.12378791004745203No Hit
AAGCCGGAATGGACTCGCGCC60.12378791004745203No Hit
AGGGGACACGTCGAGGCA60.12378791004745203No Hit
GGGACATGTCTTTAATGTTGTAGATCCGCCT60.12378791004745203No Hit
CACAGCTGCGTAGCTCTGCATTGGCCTCC60.12378791004745203No Hit
CGACTTCGTAACTTCGGCGAAGGAACTCAGCCCT60.12378791004745203No Hit
TGCCTATGGACTGCGATCGCG60.12378791004745203No Hit
TACCGGCTTGGGCTCAGC50.10315659170621003No Hit
GCAAGCCGCTCGTAGATACGGACCGCACCCT50.10315659170621003No Hit
CGACTTCGTAACTTCGGCGAAGGAACTCAGCCC50.10315659170621003No Hit
AGAAGATGTGAGGACGTGCAGAACTACGT50.10315659170621003No Hit
ATAATGTCGTGGCTCTCAGATATACAGGCCT50.10315659170621003No Hit
CCGGTTTCTGACAGCATGGCC50.10315659170621003No Hit
GAAACCACGACTGGCTGCTC50.10315659170621003No Hit
TTGTAGAGCTCGCACACC50.10315659170621003No Hit
CAAGCCGCTCGTAGATACGGACCGCACCCTGA50.10315659170621003No Hit
CTCTTCACTCGTAGCCACCTC50.10315659170621003No Hit
AACACTCCTTCGCTTC50.10315659170621003No Hit
CGCGCCGTCAGAACTGA50.10315659170621003No Hit
TCCGGAGGAACCAGTACCACCT50.10315659170621003No Hit
GTCGACACCCAGGTCCTCATC50.10315659170621003No Hit
CTCGCGCCGTCAGAACTGA50.10315659170621003No Hit
ATCAACACGGTCATTGTTCAACTCGTAAAGATCA50.10315659170621003No Hit
GAGCATCTGAGGACAAGGA50.10315659170621003No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAAGA50.007008175764.5000629
AAGATCA50.007008175764.5000629
GGCGCCA601.546141E-815.9270849
CCAGATC601.546141E-815.9270841
GATCGGG601.546141E-815.9270844
CGGGCGC601.546141E-815.9270847
ATCGGGC601.546141E-815.9270845
CAGATCG601.546141E-815.9270842
GGGCGCC601.546141E-815.9270848
AGATCGG601.546141E-815.9270843
TCGGGCG601.546141E-815.9270846
Produced by FastQC (version 0.11.5)