FastQCFastQC Report
Thu 12 Nov 2020
S33.22483.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.22483.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98
Sequences flagged as poor quality0
Sequence length26-30
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAGAAAAGCTTACTCGGCCTGTGCATC3333.6734693877551No Hit
TCGGCAACTTTTTCAGAGCGCGTGCGAAA77.142857142857142No Hit
TCAGGGGTCGGCAACTTTTTCAGAGCGC55.1020408163265305No Hit
TACATCAGGGGTCGGCAACTTTTTCAGAGC44.081632653061225No Hit
TCGGCAACTTTTTCAGAGCGCGTGCGAAAA33.061224489795918No Hit
TTCTGCTGCTAAGTCTGCTCGTACTGAAGG33.061224489795918No Hit
TACATAAGAAGCGCAATGGTTCCACCTAGG22.0408163265306123No Hit
TTCTACAAATCCTACGGAATCAAGCTCAAC22.0408163265306123No Hit
TCGTACTGAAGGAACTGGGGAAACTGTG22.0408163265306123No Hit
TCGGCTAAGTCTACACCACCATATTTGTA22.0408163265306123No Hit
TATGCCTGCCATGGCCGATCTTTTTCTTCT22.0408163265306123No Hit
GCAAACAAGAAAAAGATCGGCCATGGCAG11.0204081632653061No Hit
TACCTCAATTAAATCCTTCCACCCAAGATC11.0204081632653061No Hit
TATAAAAAATCAAACTCCACCTTACCTTT11.0204081632653061No Hit
TCCCTAGGAAATTCGTCGGCCTCACAAGT11.0204081632653061No Hit
TCTATTGCAACGACTGCACGCAAACGAGGC11.0204081632653061No Hit
TGGTCCCAACTGGTTTGTCTGATATGAGT11.0204081632653061No Hit
TCTATCGGCTAAGTCTACACCACCATATT11.0204081632653061No Hit
TACAACTGTGGAACGGAGCGATTGGGACGC11.0204081632653061No Hit
TGAAGGAACTGGGGAAACTGTGTCTGCT11.0204081632653061No Hit
ATGGTCCCAACTGGTTTGTCTGATATGAGT11.0204081632653061No Hit
TAGGAAATTCGTCGGCCACACAAGTGTG11.0204081632653061No Hit
AGGAAGCGTAAACCCAGAGAACCGTTGGC11.0204081632653061No Hit
ATCCCTAGGAAATTCGTCGGCCTCACAAGT11.0204081632653061No Hit
TACTGAAGGAACTGGGGAAACTGTGTCT11.0204081632653061No Hit
TTCCTAGGGATACAGCACTCGCACCTCCT11.0204081632653061No Hit
TAGGAAATTCGTCGGCCACACAAGTGT11.0204081632653061No Hit
TTTCCTAGGGATACAGCACTCGCACCTCCT11.0204081632653061No Hit
CGGCGTCCCAATCGCCCCGTTCCACAG11.0204081632653061No Hit
TACAACTGTGGAACGGGGCGATTGGGAC11.0204081632653061No Hit
AATACAACTGTGGAACGGGGCGATTGGGA11.0204081632653061No Hit
TGCGGTACAGGGCAATCATTCTATCGGCC11.0204081632653061No Hit
TCTACGTAGCGGAGGAGGTGCGAGTGCT11.0204081632653061No Hit
TCAGGGGTCGGCAACTTTTTCAGAGC11.0204081632653061No Hit
TAGGAAGCGTAAACCCAGAGAACCGTTGGC11.0204081632653061No Hit
TTACTCCTTTGTCGTATATACCTGTAGACT11.0204081632653061No Hit
TACATAAGAAGTGCAATGGTTCCACCTAGG11.0204081632653061No Hit
TCAAACTCCACCTTACCTTTTCGTCAATGG11.0204081632653061No Hit
TCCTACGGAATCAAGCTCAACAGACCCTTC11.0204081632653061No Hit
GCGAGTGCTGTATCCCTAGGAAATTCGT11.0204081632653061No Hit
TACAAATCCTACGGAATCAAGCTCAACAGA11.0204081632653061No Hit
TAAATTTTGCGAGGTAGACGTAACGTCGGC11.0204081632653061No Hit
TATGCCTGCCATGGCCGATCTTTTTGTTCT11.0204081632653061No Hit
CCACAAGTTCATGTTGGTATCAGCGCTAT11.0204081632653061No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)