FastQCFastQC Report
Thu 12 Nov 2020
S33.2854.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.2854.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1034
Sequences flagged as poor quality0
Sequence length15-30
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACGGCGCTGTTGGGTGGTCCCGAAGCC11110.735009671179883No Hit
TGAACCGGAACCACAGAACTCAGAGTCACT555.319148936170213No Hit
TATACGTCGTGTATGATGCGAGGCCTTGG373.5783365570599615No Hit
TAACGGCGCTGTTGGGTGGTCCCGAAGCC333.1914893617021276No Hit
TTCTAGATCTGCCGAGAACTTTCCCTTACC232.2243713733075436No Hit
TCTGAACCGGAACCACAGAACTCAGAGTC191.83752417794971No Hit
TTAACGGCGCTGTTGGGTGGTCCCGAAG181.7408123791102514No Hit
TACCCAATCGCCATGTACGCCCTCCGACT181.7408123791102514No Hit
TACGGCGGTAACAATGTCCCAGAGGCTGC151.4506769825918762No Hit
TGTTCCAGGCTCCCCACTCGTCTTCTGTG121.160541586073501No Hit
TCTAGATCTGCCGAGAACTTTCCCTTACC111.0638297872340425No Hit
TCGTCTGAACCGGAACCACAGAACTCAG100.9671179883945842No Hit
AGATACGAAACTGCATCGTGGCACTCC100.9671179883945842No Hit
TACCCTATTATCTCGTCTGTCCCCAAAGT90.8704061895551257No Hit
ACCGGAACCACAGAACTCAGAGTCACTGTT90.8704061895551257No Hit
TTTGTAGAATGGAAGTTAGCGGCGCTGTT90.8704061895551257No Hit
TTAACGGCGCTGTTGGGTGGTCCCGAAGC80.7736943907156674No Hit
TCTTATAATACGCCAGCGAAGGATCGTTCC80.7736943907156674No Hit
TTTTCAACAAGGTTCTTGGTTGGTTGGG80.7736943907156674No Hit
TGTTTGAGGACTCCACGCTGAAATGCCCT80.7736943907156674No Hit
TCAAAAAAGAAGTTCTTTCCCGCGTTGGA70.6769825918762089No Hit
TACCTGTCCACTGCTGTACGAAGGCCTCT70.6769825918762089No Hit
TTTGACTACCCAATCGCCATGTACGCCCTC70.6769825918762089No Hit
TGTTCCAGGCTCCCCACTCGTCTTCTGTGT60.5802707930367506No Hit
TAACGGCGCTGTTGGGTGGTCCCGAAG60.5802707930367506No Hit
TTGTAGAATGGAAGTTAGCGGCGCTGTT60.5802707930367506No Hit
TAGGCCAGGAAATTGTTCATCGTCTGAACC60.5802707930367506No Hit
TACGCCCTCCGACTTGAAGGTACACAGCCT60.5802707930367506No Hit
TCAGCCGTTACCTGTCCACTGCTGGACGAA60.5802707930367506No Hit
TTGTCGCATTAAGAAAAGATTCCCTACCC60.5802707930367506No Hit
TTCTAGATCTGCCGAGAACTTTCCCTTAC60.5802707930367506No Hit
TCCCTTACCATACTTCCACGACAAACAGCT60.5802707930367506No Hit
TATACGTCGTGTATGATGCGAGGCCTTGGA60.5802707930367506No Hit
TCGTCTGAACCGGAACCACAGAACTCAGA60.5802707930367506No Hit
TTATACAACCTCCCCACCAATAGCCAACAT50.4835589941972921No Hit
TGAGGACTCCACGCTGAAATGCCCTGACCC50.4835589941972921No Hit
TTTTTCAACAAGGTTCTTGGTTGGTTGGG50.4835589941972921No Hit
TCAGAGTCACTGTTTGCTGAGGCAATCTT50.4835589941972921No Hit
TCCCAGAGGCTGCTCCGACAAACATTACAT50.4835589941972921No Hit
TGAGGACTCCACGCTGAAATGCCCTGACC50.4835589941972921No Hit
CACTTCAACTACCTCGCCAACTGTTATCGC50.4835589941972921No Hit
TTTTCAACAAGGTTCTTGGTTGGTTGGGA40.3868471953578337No Hit
GAGGACTCCACGCTGAAATGCCCTGACCCG40.3868471953578337No Hit
TGATTGTCGTCGTCGTGGTCAGCCGTTA40.3868471953578337No Hit
TAGGCACAATCGTGGACGACGTGGAGGT40.3868471953578337No Hit
CCGGTTCAGACGATGAACAATTTCCTGGC40.3868471953578337No Hit
AACCACAGAACTCAGAGTCACTGTTTGCTG40.3868471953578337No Hit
TGTTGTATCGACACCCGGCTGGGCCTCTC40.3868471953578337No Hit
TGTTAACAAGTGTTCCAGGCTCCCCACTC40.3868471953578337No Hit
TAAAAGGATCTGTTCGGGTAATGAGTGTC40.3868471953578337No Hit
TCCACGCTGAAATGCCCTGACCCGCCT40.3868471953578337No Hit
GAACCGGAACCACAGAACTCAGAGTCACT40.3868471953578337No Hit
TTACCTAAATTTCCTCTACCATTCCTCGG40.3868471953578337No Hit
TCGGTTATTACTCTAAATGCCCCGCAAAAC40.3868471953578337No Hit
AGATACGAAACTGCATCGTGGCACTCCT40.3868471953578337No Hit
AGATACGAAACTGCATCGTGGCACTC40.3868471953578337No Hit
TGCGCCGAACCCGTTACATAGTTAGATCTC40.3868471953578337No Hit
TGCAGAAATACCGCTGGCAGAAGCTCCACT40.3868471953578337No Hit
TCCTGGCCTATCTGTTTCGTGTGGACGC40.3868471953578337No Hit
TACGCCAGCGAAGGATCGTTCCCTAAACTC40.3868471953578337No Hit
TAGGCCAGGAAATTGTTCATCGTCTGAAC40.3868471953578337No Hit
TCCGAATCGATATTGTCGAATAACCCACCT40.3868471953578337No Hit
TAGAATACAGCACCAGCACCAGCAGATGA30.2901353965183753No Hit
TGAACCGGAACCACAGAACTCAGAGTCAC30.2901353965183753No Hit
TACGTCGTGTATGATGCGAGGCCTTGG30.2901353965183753No Hit
AAAAAGCACAGGATAGAACTCAATGGAGAG30.2901353965183753No Hit
TACGTCGTGTATGATGCGAGGCCTTGGA30.2901353965183753No Hit
TTAGAGAGAGGACCCAACAACATTGTCGC30.2901353965183753No Hit
TACCCAATCGCCATGTACGCCCTCCGACTT30.2901353965183753No Hit
TAATGAGTGTCTTTTCTGAGTGGTCCCACC30.2901353965183753No Hit
GACGCCTCGACAAACAGTGGAGCTTCTGCC30.2901353965183753No Hit
TACGTCGTGTATGATGCGAGGCCTTGGAG30.2901353965183753No Hit
TCATCTTCTCCACCTAGTCCCTCAAATCT30.2901353965183753No Hit
TTCCCTACCCGGATGTATCTGTTTATCGAC30.2901353965183753No Hit
TACCTACCAAGCCGAAGTTACAATAAAAAT30.2901353965183753No Hit
TTCCCTTACCATACTTCCACGACAAACAGC30.2901353965183753No Hit
CAGGCTCCCCACTCGTCTTCTGTGT30.2901353965183753No Hit
ATCTAGAACACCAGGTCACCCCGAGC30.2901353965183753No Hit
TACATATACCCTATTATCTCGTCTGTCCCC30.2901353965183753No Hit
TATGGACACCCGGCTGGGCCTCTCTCA30.2901353965183753No Hit
ACCGGAACCACAGAACTCAGAGTCACTGT30.2901353965183753No Hit
TCCTTCTTGTTCTTCGTGGCGGCAGCCGA30.2901353965183753No Hit
TTCCACGAGTGCCACATAATCCCTAAGGG30.2901353965183753No Hit
TTGAGGACTCCACGCTGAAATGCCCTGACC30.2901353965183753No Hit
TTATCTCGTCTGTCCCCAAAGTACATAATT30.2901353965183753No Hit
TATACCCTATTATCTCGTCTGTCCCCAAAG30.2901353965183753No Hit
TAAGTTCGGTACGACTTTCGGAAGAGGT30.2901353965183753No Hit
TACACGTGCCTCGCGATAACAGTTGGCG30.2901353965183753No Hit
TATAGAATACAGCACCAGCAGCAGCAGAT30.2901353965183753No Hit
TATAGAATACAGCACCAGCAGCAGCAGATG20.19342359767891684No Hit
TGTTTGATCCCCACCAAGTAACGTTTTCA20.19342359767891684No Hit
ATGGAGAGGAAATGAAGAGGCCTTGGTC20.19342359767891684No Hit
TCCCTAATGAACGGAGCCAGCTGCATCTC20.19342359767891684No Hit
TTACCTAAATTTCCTCTACCATTCCTGGG20.19342359767891684No Hit
TTCCCTTACCATACTTCCACGACAAACAG20.19342359767891684No Hit
GTGGCTCCCCACTCGTC20.19342359767891684No Hit
TCCCGTTGTTAACAAGTGTTCCAGGCTCCC20.19342359767891684No Hit
TGAGTGTCTTTTCTGAGTGGTCCCACCTC20.19342359767891684No Hit
TCCTTAATGTGAAGTGAAGACCATACCCTG20.19342359767891684No Hit
CCTCGACAAACAGTGGAGCTTCTGCCAGC20.19342359767891684No Hit
GCGCCGCTAACTTCCATTCTACAAACTC20.19342359767891684No Hit
TTGATTGTCGTCGTCGTGGTCAGCCGTT20.19342359767891684No Hit
TACCTGTTGAAATTACCTACCAAGCCGAAG20.19342359767891684No Hit
TGTTGTATCGACACCCGGCTGGGCCTCTCC20.19342359767891684No Hit
TTGAAAAATATGTTTCCCGGATATTGCCC20.19342359767891684No Hit
TACCCGGATGTATCTGTTTATCGACTAAGT20.19342359767891684No Hit
TACTTCCACGACAAACAGCTGACCGCTCTT20.19342359767891684No Hit
TATAGAATACAGCACCAGCACCAGCAGAT20.19342359767891684No Hit
TGTGTAGGCGCAATCGTGGACGACGTGG20.19342359767891684No Hit
TGGGTCGAGTACGGAGGGCGTCTTGTATGC20.19342359767891684No Hit
TAAGAAAAGATTCCCTACCCGGATGTATC20.19342359767891684No Hit
TCCCTACCCGGATGTATCTGTTTATCGACT20.19342359767891684No Hit
TTTACCTAAATTTCCTCTACCATTCCTCGG20.19342359767891684No Hit
TCCTGGCCTATCTGTTTCGTGTGGACGCC20.19342359767891684No Hit
ACAGATACGAAACTGCATCGTGGCACTC20.19342359767891684No Hit
TGAAGACCATACCCTGTACCAAAAGAATA20.19342359767891684No Hit
TATCGACACCCGGCTGGGCCTCTCTCAGT20.19342359767891684No Hit
AACATGGTCTCTATTCTCAGCTGCAAGGG20.19342359767891684No Hit
TGACCGCTCTTATCAACTTCTCCGAATACC20.19342359767891684No Hit
TCAAACACTACTCCGTTGAACCATCCGACC20.19342359767891684No Hit
TCTTGCCACCAGTCCTTCTTGTTCTTCGT20.19342359767891684No Hit
TGAACCGGAATCACAGAACTCAGAGTCACT20.19342359767891684No Hit
TCCATTACCCTATCCGGATGCTTGCAGCCT20.19342359767891684No Hit
TCTTTTCTGAGTGGTCCCACCTCCCCCGCC20.19342359767891684No Hit
TATCCGGATGCTTGCAGCCTCTTTTAACA20.19342359767891684No Hit
TTTGACTACCCAATCGCCATGTACGCCC20.19342359767891684No Hit
TGAACGGAGCCAGCTGCATCTCACCCTCTT20.19342359767891684No Hit
TAGAATGGGCGGATTCTGTCATTGACA20.19342359767891684No Hit
TTATAATACGCCAGCGAAGGATCGTTCCC20.19342359767891684No Hit
TTCAACGTTTCTAAGGCCGTCGGTTCAGCT20.19342359767891684No Hit
TCTTCATTTCCTCTCCATTGAGTTCTATCC20.19342359767891684No Hit
TGTTTGAGGACTCCACGCTGAAATGCCC20.19342359767891684No Hit
TTCCCTAAACTCGCGTTCATTTCTGCCCT20.19342359767891684No Hit
AACGGCGCTGTTGGGTGGTCCCGAAGCC20.19342359767891684No Hit
TTTTCCGATCCTCTTTTTATACAACCTCCC20.19342359767891684No Hit
AAGCACAGGATAGAACTCAATGGAAGT20.19342359767891684No Hit
TACGCCCTCCGACTTGAAGGTATACAGCCT20.19342359767891684No Hit
TCCTGGCCTATCTGTTTCGTGTGGACG20.19342359767891684No Hit
TCATAACTCTTTCTAGCCATGTCCGCATA20.19342359767891684No Hit
TAATGAGTGTCTTTTCTGAGTGGTCCCA20.19342359767891684No Hit
AACGATCCGACCTGTTGTATCGACACCCGG20.19342359767891684No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)