FastQCFastQC Report
Thu 12 Nov 2020
S34.22483.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.22483.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244
Sequences flagged as poor quality0
Sequence length16-30
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAGAAAAGCTTACTCGGCCTGTGCATC5422.131147540983605No Hit
TGCAAGCAGTGAAGCCGCAGCCTTACGGAC156.147540983606557No Hit
TAGGAAATTCGTCGGCCTCACAAGTGTGG93.6885245901639343No Hit
TCGGCAACTTTTTCAGAGCGCGTGCGAAA93.6885245901639343No Hit
TACAACTGTGGAACGGGGCGATTGGGA62.459016393442623No Hit
TCAGGGGTCGGCAACTTTTTCAGAGCGC52.0491803278688523No Hit
TAAGGATGCAGCATCCAATGCAACGTGTAA41.639344262295082No Hit
GCAAGCAGTGAAGCCGCAGCCTTACGGAC41.639344262295082No Hit
TAAATTTTGCGAGGTAGACGTAACGTCGGC41.639344262295082No Hit
TACAACTGTGGAACGGGGCGATTGGGACG41.639344262295082No Hit
TAGGAAATTCGTCGGCCTCACAAGTGTGGA31.2295081967213115No Hit
TCAAACTCCACCTTACCTTTTCGTCAATGG31.2295081967213115No Hit
TCAAGCTCAACAGACCCTTCTATTGCAAC31.2295081967213115No Hit
TACAACTGTGGAACGGGGCGATTGGGAC31.2295081967213115No Hit
TCCCTAGGAAATTCGTCGGCCTCACAAGT31.2295081967213115No Hit
AAGAAAAAGATCGGCCATGGCAGGCA31.2295081967213115No Hit
TCTGCTCGTACTGAAGGAACTGGGGAAACT31.2295081967213115No Hit
TTTTCAGAGCGCGTGCGAAAACAGCTTT31.2295081967213115No Hit
TTTTGCGAGGTAGACGTAACGTCGTCGGC31.2295081967213115No Hit
TGCGAGGTAGACGTAACGTCGTCGGCTT20.819672131147541No Hit
TGCTGTATCCCTAGGAAATTCGTCGGCCTC20.819672131147541No Hit
TGCTCGTACTGAAGGAACTGGGGAAACTGT20.819672131147541No Hit
TAGTCAAGTCCAAGCCTACCTAGACACA20.819672131147541No Hit
TCCTACAGAATCAAGCTCAACAGACCCTT20.819672131147541No Hit
GTTTTTGCACGCGCTCTGAAAAAGTT20.819672131147541No Hit
TACAACTGTGGAACGGGGCGATTGGG20.819672131147541No Hit
TGCTGTATCCCTAGGAAATTCGTCGGCC20.819672131147541No Hit
TACGTAGCGGAGGAGGTGCGAGTGCTGT20.819672131147541No Hit
TTATACTTCATGCGGTACAGGGCAATCATT20.819672131147541No Hit
TTATTACTCCTTTGTCGTATATACCTGTA20.819672131147541No Hit
TCAGGGGTCGGCAACTTTTTCAGAGCGCG20.819672131147541No Hit
ATCAGGGGTCGGCAAC20.819672131147541No Hit
AGCAAACAAGAAAAAGATCGGCCATGGC20.819672131147541No Hit
TGTATTGTTCCCTATCATCGAGCTTCAGG20.819672131147541No Hit
TCGTACTGAAGGAACTGGGGAAACTGTG20.819672131147541No Hit
TGCTGTATCCCTAGGAAATTCGTCGGCCT20.819672131147541No Hit
TGCGTGCAGTCGTTGCAATAGAAGGGTC20.819672131147541No Hit
TACAACTGTGGAACGGGGCGATTGGGACGT20.819672131147541No Hit
AATCCTACAGAATCAAGCTCAACAGACCCT20.819672131147541No Hit
TAGGGATACAGCACTCGCACC20.819672131147541No Hit
ATCATTCTATCGGCCAAGTCTACACCACC10.4098360655737705No Hit
ATCCCTAGGAAATTCGTCGGCCTCACAAGA10.4098360655737705No Hit
AAGTTGCCGACCCCTG10.4098360655737705No Hit
TCGTATATACCTGTAGACTATTTGCCAT10.4098360655737705No Hit
TACGTAGCGGAGGAGGTGCGAGTGCTGTAA10.4098360655737705No Hit
TTGTCACATTCGGCCGCTGTACTGGTC10.4098360655737705No Hit
TGAAGCCGCAGCCTTACGGACCAGTACAG10.4098360655737705No Hit
CGCGCTCTGAAAACGTTGCCGACCCCTG10.4098360655737705No Hit
ATCCCTAGGAAATTCGTCGGCCTCACAAGT10.4098360655737705No Hit
CTCTGAAAAAGTTGCCGACCCCTGGTC10.4098360655737705No Hit
AAGAAAAAGATCGGCCATGGCAGG10.4098360655737705No Hit
TACGTAGCGGAGGAGGTGCGAGTGCTGTA10.4098360655737705No Hit
TTGGGAGTGAGATCGGGGTTTGATATTG10.4098360655737705No Hit
TCCTACAGAATCAAGCTCAACAGACCCTTC10.4098360655737705No Hit
TGAGGCCGACGAATTTCCTAGGGATACAGC10.4098360655737705No Hit
TATTGCAACGACTGCACGCAAACGAGGCTC10.4098360655737705No Hit
GAGGACATGGATACCAGCCACAATGTCGGC10.4098360655737705No Hit
TGTGTACTATTCATTAGATCAGGGGTCGGT10.4098360655737705No Hit
TTATACTTCATGCGGTACAGGGCAATCAT10.4098360655737705No Hit
TGTGTACTATTCATTAGATCAGGGGTCGGC10.4098360655737705No Hit
TAGAAGAGCAAACAAGAAAAAGATCGGCC10.4098360655737705No Hit
AGGAAGCGTAAACCCAGAGAACCGTTGGC10.4098360655737705No Hit
TCGCTTGATCGCCAACGGTTCTCTGGGT10.4098360655737705No Hit
TACAACTGTGGAACGGGGCGATTGGGAA10.4098360655737705No Hit
TTTGTCTGATATGAGTATGGTGGCGTT10.4098360655737705No Hit
TGTTCAAAATATGACTGCGCACGCGAGCTC10.4098360655737705No Hit
TCGTACTGAAGGAACTGGGGAAACTGTGT10.4098360655737705No Hit
TAAGTCTGCTCGTACTGAAGGAACTGGGG10.4098360655737705No Hit
TAGGAAGCGTAAACCCAGAGAACCGTTGGC10.4098360655737705No Hit
TCGTGTCAGGTGAAAATATTGGGAGTGAG10.4098360655737705No Hit
AACTTTTTCAGAGCGCGTGCGAAAACAGC10.4098360655737705No Hit
TCTGCTGCTAAGTCTGCTCGTACTGAAGG10.4098360655737705No Hit
TACAGAATCAAGCTCAACAGACCCTTCTAT10.4098360655737705No Hit
TTGGGACGCCGACACGAAAAAGAACG10.4098360655737705No Hit
TGCTACGTTACCCCCTAATACTTGGATT10.4098360655737705No Hit
ACCCTGTTGAGCTTGATTCT10.4098360655737705No Hit
TACAACTGTGGAACGGAGCGATTGGGACGC10.4098360655737705No Hit
TATCCCTAGGAAATTCGTCGGCCACACAAG10.4098360655737705No Hit
AATAGCAGACACAGTTTCCCCAGTTCCTTC10.4098360655737705No Hit
TCAAACTCCACCTTACCTTTTCGTCAATG10.4098360655737705No Hit
GAAATTCGTCGGCCTCACAAGTGTGGAC10.4098360655737705No Hit
TTCTGCTGCTAAGTCTGCTCGTACTGAAGG10.4098360655737705No Hit
ACGATCCTCTTCTACAAATCCTACAGAAT10.4098360655737705No Hit
TTTGTCACATTCGGCCGCTGTACTGG10.4098360655737705No Hit
TGTTTGAAGAGCCTCGTTTGCGTGCAGTC10.4098360655737705No Hit
AAAGCACCGGATTCTATGTATTGTTCCCTA10.4098360655737705No Hit
TACGTAGCGGAGGAGGTGCGAGTGCTG10.4098360655737705No Hit
TGCTGTATCCCTAGGAAATTCGTCGGCCC10.4098360655737705No Hit
TCTATTGCAACGACTGCACGCAAACGAGG10.4098360655737705No Hit
CAAAATATGACTGCGCCCGCGAGCT10.4098360655737705No Hit
TACATAAGAAGCGCAATGGTTCCACCTAGG10.4098360655737705No Hit
TCTGCTCGTACTGAAGGAACTGGGGAAA10.4098360655737705No Hit
TGCACGCAAACGAGGCTCTTCAAACACAGG10.4098360655737705No Hit
AACTGTAGCACCAATATCAAACCCCGATCT10.4098360655737705No Hit
CGGCAACTTTTTCAGAGCGCGTGCGAAAAC10.4098360655737705No Hit
TTGGGACGCCGACACGAAAAAGAACGTCT10.4098360655737705No Hit
TTTAGCAAATCTCGTGTGTGGTACTCAT10.4098360655737705No Hit
TTGGGAGTGAGATCGGGGTTTGATATTGGT10.4098360655737705No Hit
AGGGGTCGGCAACTTTTTC10.4098360655737705No Hit
TTTAGCAAATCTCGTGTGTGGTACTCATA10.4098360655737705No Hit
ACAACTGTGGAACGGGGCGATTGGGACGC10.4098360655737705No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)