FastQCFastQC Report
Thu 12 Nov 2020
S34.2854.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.2854.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1679
Sequences flagged as poor quality0
Sequence length15-30
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATACGTCGTGTATGATGCGAGGCCTTGG1227.266229898749256No Hit
TGAACCGGAACCACAGAACTCAGAGTCACT784.645622394282311No Hit
TGTTCCAGGCTCCCCACTCGTCTTCTGTG774.586063132817153No Hit
TATACGTCGTGTATGATGCGAGGCCTTGGA513.0375223347230493No Hit
TACGGCGGTAACAATGTCCCAGAGGCTGC372.20369267421084No Hit
TAGGCACAATCGTGGACGACGTGGAGGT331.9654556283502083No Hit
TACCCAATCGCCATGTACGCCCTCCGACT291.7272185824895772No Hit
TCTGAACCGGAACCACAGAACTCAGAGTC271.6081000595592614No Hit
TCTAGATCTGCCGAGAACTTTCCCTTACC271.6081000595592614No Hit
AGATACGAAACTGCATCGTGGCACTCC201.1911852293031566No Hit
TACCCTATTATCTCGTCTGTCCCCAAAGT191.1316259678379987No Hit
TTCTAGATCTGCCGAGAACTTTCCCTTACC191.1316259678379987No Hit
TACGTCGTGTATGATGCGAGGCCTTGG181.0720667063728408No Hit
AGATACGAAACTGCATCGTGGCACTC181.0720667063728408No Hit
TGTTTGAGGACTCCACGCTGAAATGCCCT181.0720667063728408No Hit
TCTTATAATACGCCAGCGAAGGATCGTTCC160.9529481834425254No Hit
GAGGACTCCACGCTGAAATGCCCTGACCCG150.8933889219773675No Hit
TTTGTAGAATGGAAGTTAGCGGCGCTGTT150.8933889219773675No Hit
TCGTCTGAACCGGAACCACAGAACTCAG150.8933889219773675No Hit
TGTTCCAGGCTCCCCACTCGTCTTCTGTGT150.8933889219773675No Hit
AGCTTGGGGACAGATACGAAACTGCATC150.8933889219773675No Hit
TCCACGCTGAAATGCCCTGACCCGCCT150.8933889219773675No Hit
ACCGGAACCACAGAACTCAGAGTCACTGTT140.8338296605122096No Hit
AGATACGAAACTGCATCGTGGCACTCCT140.8338296605122096No Hit
TTATACAACCTCCCCACCAATAGCCAACAT110.6551518761167362No Hit
TAGGCCAGGAAATTGTTCATCGTCTGAACC110.6551518761167362No Hit
TAAGTTCGGTACGACTTTCGGAAGAGGT110.6551518761167362No Hit
TGAACCGGAACCACAGAACTCAGAGTCAC100.5955926146515783No Hit
TCAAAAAAGAAGTTCTTTCCCGCGTTGGA100.5955926146515783No Hit
CCGGTTCAGACGATGAACAATTTCCTGGC100.5955926146515783No Hit
TCCCTACCCGGATGTATCTGTTTATCGACT100.5955926146515783No Hit
TACGTCGTGTATGATGCGAGGCCTTGGAG100.5955926146515783No Hit
TGAACGGAGCCAGCTGCATCTCACCCTCTT90.5360333531864204No Hit
TTCTAGATCTGCCGAGAACTTTCCCTTAC90.5360333531864204No Hit
TCCCTAAACTCGCGTTCATTTCTGCCCT90.5360333531864204No Hit
TCCTGGCCTATCTGTTTCGTGTGGACGC80.4764740917212627No Hit
TCTGCCGAGAACTTTCCCTTACCATACTTC80.4764740917212627No Hit
TGCAGAAATACCGCTGGCAGAAGCTCCACT80.4764740917212627No Hit
AAGGTATTGACGACACGACTGGTTCCGAAT80.4764740917212627No Hit
TTGTAGAATGGAAGTTAGCGGCGCTGTT70.4169148302561048No Hit
TACCTGTCCACTGCTGTACGAAGGCCTCT70.4169148302561048No Hit
TTCCCTAAACTCGCGTTCATTTCTGCCCT70.4169148302561048No Hit
TGCGCCGAACCCGTTACATAGTTAGATCTC70.4169148302561048No Hit
TTCCCTTACCATACTTCCACGACAAACAG60.357355568790947No Hit
TGTGTCTCAATATCGACAACGTGCACCGGT60.357355568790947No Hit
TATACGGCGGTAACAATGTCCCAGAGGCT60.357355568790947No Hit
TTAGCGGCGCTGTTGGGTGGTCCCGAAG60.357355568790947No Hit
TGTTGTATCGACACCCGGCTGGGCCTCTC60.357355568790947No Hit
TACGTCGTGTATGATGCGAGGCCTTGGA50.29779630732578916No Hit
TTGATTGTCGTCGTCGTGGTCAGCCGTT50.29779630732578916No Hit
TGAGTGTCTTTTGTGAGTGGTCCCACCTC50.29779630732578916No Hit
TACCCTATTATCTCGTCTGTCCCCAAAG50.29779630732578916No Hit
TACGCCCTCCGACTTGAAGGTACACAGCCT50.29779630732578916No Hit
TATAGAATACAGCACCAGCAGCAGCAGAT50.29779630732578916No Hit
TTTGACTACCCAATCGCCATGTACGCCCTC50.29779630732578916No Hit
TGATTGTCGTCGTCGTGGTCAGCCATTACC50.29779630732578916No Hit
TACCCTATCCGGATGCTTGCAGCCTCTT50.29779630732578916No Hit
TAGTTGCTCCAGAATTGATAGACTGTCCTC50.29779630732578916No Hit
TAGGCCAGGAAATTGTTCATCGTCTTTGGC50.29779630732578916No Hit
TCGTCTGAACCGGAACCACAGAACTCAGA50.29779630732578916No Hit
TTGTCGCATTAAGAAAAGATTCCCTACCCG50.29779630732578916No Hit
TAAAAGGATCTGTTCGGGTAATGAGTGT50.29779630732578916No Hit
TCAACGTTTCGAAGGCCGTCGGTTCAGCT40.23823704586063135No Hit
TTCGAAGGCCGTCGGTTCAGCTTGTGGT40.23823704586063135No Hit
TCCATCAGGGCTTTGTTGTACACTTCGGT40.23823704586063135No Hit
TAGAATGGAAGTTAGCGGCGCTGTTGGG40.23823704586063135No Hit
CCTCGACAAACAGTGGAGCTTCTGCCAGC40.23823704586063135No Hit
TCAACTTCTCCGAATACCCGTTGGCGTCT40.23823704586063135No Hit
TCCAGGCTCCCCACTCGTCTTCTGTGTT40.23823704586063135No Hit
TTCCACGAGTGCCACATAATCCCTAAGGG40.23823704586063135No Hit
TTATAATACGCCAGCGAAGGATCGTTCCC40.23823704586063135No Hit
TTATCTCGTCTGTCCCCAAAGTACATAATT40.23823704586063135No Hit
TACCTAAATTTCCTCTACCATTCCTCGGAC40.23823704586063135No Hit
TTGTTAACAAGTGTTCCAGGCTCCCCACTC40.23823704586063135No Hit
ATGGTCTCTATTCTCAGCTGCAAGGGTT40.23823704586063135No Hit
TGATTGTCGTCGTCGTGGTCAGCCGTTA40.23823704586063135No Hit
TAGATCTGCCGAGAACTTTCCCTTACCAT40.23823704586063135No Hit
TAATACGCCAGCGAAGGATCGTTCCCTAAA40.23823704586063135No Hit
TCCTGGCCTATCTGTTTCGTGTGGACGCC40.23823704586063135No Hit
TTTGACTACCCAATCGCCATGTACGCCCT40.23823704586063135No Hit
TGTATCGACACCCGGCTGGGCCTCTCTCAG40.23823704586063135No Hit
TTCGCGAAATCGTCTCCCTTGTAAATTGT40.23823704586063135No Hit
TCAAGGAGGTCCAAATTTCTTTCGGAATG40.23823704586063135No Hit
TCCATTACCCTATCCGGATGCTTGCAGCCT40.23823704586063135No Hit
TTTCCCTTACCATACTTCCACGACAAACAG40.23823704586063135No Hit
TGTACCTGTTTCCTGAGATGTACTTGCCT40.23823704586063135No Hit
TCAGAGTCACTGTTTGCTGAGGCAATCTT40.23823704586063135No Hit
TCTGAACCGGAACCACAGAACTCAGAGTCA40.23823704586063135No Hit
CAGATCTAGAACACCAGGTCACCCCGAGC40.23823704586063135No Hit
TGAACCGGAACCACAGAACTCAGAGTC30.1786777843954735No Hit
TCCCTAATGAACGGAGCCAGCTGCATCTC30.1786777843954735No Hit
ATCTAGAACACCAGATCACCCCGAGCA30.1786777843954735No Hit
TAACAATGTCCCAGAGGCTGCTCCGACA30.1786777843954735No Hit
TCCAGGCTCCCCACTCGTCTTCTGTGT30.1786777843954735No Hit
TTTTTCAACAAGGTTCTTGGTTGGTTGGG30.1786777843954735No Hit
TATCGACACCCGGCTGGGCCTCTCTCAGT30.1786777843954735No Hit
TTCCTTCTGGTGACCTGACGTTTCAACTCC30.1786777843954735No Hit
TCCCTTACCATACTTCCACGACAAACAGCT30.1786777843954735No Hit
TCTGTAATGTTTCGTTCCTTCTGGTGACCT30.1786777843954735No Hit
TCATCGTCTGAACCGGAACCACAGAACTCA30.1786777843954735No Hit
TTATCAACTTCTCCGAATACCCGTTGGC30.1786777843954735No Hit
TCAGACCTTCCGCAATCCAGTTTGTTTCCT30.1786777843954735No Hit
ACCGGAACCACAGAACTCAGAGTCACT30.1786777843954735No Hit
AGGACGTGGAGTTGAAACGTCAGGTCACC30.1786777843954735No Hit
TTCTGACAACCTAACATAATCCCTA30.1786777843954735No Hit
TTAGATTCTCATGGTCCAAGGAAACATACT30.1786777843954735No Hit
TCCTGGGACACAAGATAGAATCTGCTCTAA30.1786777843954735No Hit
TTGTATCGACACCCGGCTGGGCC30.1786777843954735No Hit
TGCAGAAATACCGCTGGCAGAAGCTCCAC30.1786777843954735No Hit
TCAATATCGACAACGTGCACCGGTGCAAGG30.1786777843954735No Hit
TCTCATGGTCCAAGGAAACATACTTATTG30.1786777843954735No Hit
TATCGACACCCGGCTGGGCCTCTCTCA30.1786777843954735No Hit
TGAAAGGAAACAAACTGGATTGCGGAAGGT30.1786777843954735No Hit
TTGCGGCGAATTCGGCATTGGGTCGTGC30.1786777843954735No Hit
AGGCACAATCGTGGACGACGTGGAGGT30.1786777843954735No Hit
TGACTACCCAATCGCCATGTACGCCCTCC30.1786777843954735No Hit
TCTTATAATACGCCAGCGAAGGATCGTTC30.1786777843954735No Hit
TCCGGATGCTTGCAGCCTCTTTTAACAAG30.1786777843954735No Hit
TGCTCTCTACTATCTTATAATACGCCAG30.1786777843954735No Hit
TACGTCGTGTATGATGCGAGGCCTTGGAA30.1786777843954735No Hit
ATGGTCTCTATTCTCAGCTGCAAGGGTTGT30.1786777843954735No Hit
TATACGTCGTGTATGATGCGAGGCCTTG30.1786777843954735No Hit
ATCTAGAACACCAGGTCACCCCGAGC30.1786777843954735No Hit
TCCTTCTGGTGACCTGACGTTTCAACTCC30.1786777843954735No Hit
ACCGGAACCACAGAACTCAGAGTCACTGT30.1786777843954735No Hit
TCTGCCGAGAACTTTCCCTTACCATACTT30.1786777843954735No Hit
TGTGACACCACCAAACTTTCTCACGTCGTC30.1786777843954735No Hit
TCCACCTAGTCCCTCAAATCTACGATCATA30.1786777843954735No Hit
TATCAACTTCTCCGAATACCCGTTGGCGTC30.1786777843954735No Hit
TTGGAGATAGAGCAAGGTATTGACGACACA30.1786777843954735No Hit
AAGGTATTGACGACACGACTGGTTCCGAA20.11911852293031568No Hit
TTGTAGAATGGAAGTTAGCGGCGCTG20.11911852293031568No Hit
TTCAACTCCACGTCCTGTGTGCGTCGTG20.11911852293031568No Hit
TCAGAGTCACTGTTTGCTGAGGCAATT20.11911852293031568No Hit
TAGAATACAGCACCAGCACCAGCAGATGA20.11911852293031568No Hit
TCATTTCGTCGGTTATTACTCTAAATGCC20.11911852293031568No Hit
TCGCCGTGAAAGGAAACAAACTGGATTGC20.11911852293031568No Hit
ACCTAAATTTCCTCTACCATTCCTGGGAC20.11911852293031568No Hit
TGAAGACCATACCCTGTACCAAAAGAAT20.11911852293031568No Hit
CGGAACCACAGAACTCAGAGTCACT20.11911852293031568No Hit
TCAACTTCTCCGAATACCCGTTGGCGTC20.11911852293031568No Hit
CCTAATGAACGGAGCCAGCTGCATCTCACC20.11911852293031568No Hit
TTGTATCGACACCCGGCTGGGCCTCTCA20.11911852293031568No Hit
TGTTCCAGGCTCCCCACTCGTCTTCTGT20.11911852293031568No Hit
TCCTGGGACACAAGATAGAATCTGCTCTA20.11911852293031568No Hit
TCTTATAATACGCCAGCGAAGGATCGT20.11911852293031568No Hit
TTATCCCGTAGGTTGTGGAAAAAGGCTA20.11911852293031568No Hit
TCCTGGCCTATCTGTTTCGTGTGGAC20.11911852293031568No Hit
TGTTGTATCGACACCCGGCTGGGCCTCT20.11911852293031568No Hit
ATGGTCTCTATTCTCAGCTGCAAGGGT20.11911852293031568No Hit
ATTGTGACATGAGGACCGTCACCGGTTACT20.11911852293031568No Hit
TTGTATCGACACCCGGCTGGGCCTCTCT20.11911852293031568No Hit
AGCACAGGATAGAACTCAATGGAGAGG20.11911852293031568No Hit
TGAGTGTCTTTTGTGAGTGGTCCCACC20.11911852293031568No Hit
TTTTCAACAAGGTTCTTGGTTGGTTGGG20.11911852293031568No Hit
TCTACTTCCGACATCTTGTTTACATTCTCC20.11911852293031568No Hit
TTCCTCTCCATGGAGTTCTATCCTGTGCT20.11911852293031568No Hit
TTCCTCTCCATTGAGTTCTATCCTGTGCT20.11911852293031568No Hit
TACATATACCCTATTATCTCGTCTGTCCCC20.11911852293031568No Hit
TTATAGAATACAGCACCAGCACCAGCAGAT20.11911852293031568No Hit
GAGGACCCAACAACATTGTCGCATTAAGAA20.11911852293031568No Hit
TGCCGAATTCGCCGCAAAAGTCACCGTCGT20.11911852293031568No Hit
TCGACAACGTGTACCGGTGCAAGGGCCC20.11911852293031568No Hit
TCCCAGAGGCTGCTCCGACAAACATTACAT20.11911852293031568No Hit
TCTGATAGTTGATTGTCGTCGTCGTGGTC20.11911852293031568No Hit
TGTTTGAGGACTCCACGCTGAAATGCCC20.11911852293031568No Hit
TAACGCGAACTATTGTTTGATCCCCACC20.11911852293031568No Hit
TGCTGGACGTTCGCAAAACTTTTGTGCCA20.11911852293031568No Hit
TTCCTCTCCATTGAGTTCTATCCTGTG20.11911852293031568No Hit
TCCATTACCCTATCCGGATGCTTGCAGCC20.11911852293031568No Hit
AGATACGAAACTGCATCGTGGCACTT20.11911852293031568No Hit
TTACCCTATCCGGATGCTTGCAGCCTCTT20.11911852293031568No Hit
TTTGAGGACTCCACGCTGAAATGCCA20.11911852293031568No Hit
TTTTCAACAAGGTTCTTGGTTGGTTGGGA20.11911852293031568No Hit
TGACTACCCAATCGCCATGTACGCCCTCCG20.11911852293031568No Hit
TGTAGAATGGAAGTTAGCGGCGCTGTT20.11911852293031568No Hit
TGAACCGGAACCACAGAACTCAGAGTCA20.11911852293031568No Hit
AAAAAGCACAGGATAGAACTCAATGGAGAG20.11911852293031568No Hit
TGTTTGAGGACTCCACGCTGAAATGC20.11911852293031568No Hit
TGAAGAGGACTTCGTCCAGCAGTGGACAG20.11911852293031568No Hit
TTACCATACTTCCACGACAAACAGCTGACC20.11911852293031568No Hit
TGTAATGTTTCGTTCCTTCTGGTGACCTG20.11911852293031568No Hit
TCTATCAAGGAGGTCCAAATTTCTTTCGG20.11911852293031568No Hit
TTCGGTACGACTTTCGGAAGAGGTAAC20.11911852293031568No Hit
TGTGAAGTGAAGACCATACCCTGTACCAA20.11911852293031568No Hit
GAACAAGAAGGACTGGTGGCAAGAAGCCA20.11911852293031568No Hit
CACAGAACTCAGAGTCACTGTTTG20.11911852293031568No Hit
TGATTGTCGTCGTCGTGGTCAGCCGTT20.11911852293031568No Hit
AGATGGCTCACAACCCTTGCAGCTGAGA20.11911852293031568No Hit
TGAGGACTCCACGCTGAAATGCCCTGACCC20.11911852293031568No Hit
TATAGAATACAGCACCAGCACCAGCAG20.11911852293031568No Hit
TTCGAAGGCCGTCGGTTCAGCTTGTGGTT20.11911852293031568No Hit
TTTTATACAACCTCCCCACCAATAGCCAAC20.11911852293031568No Hit
TACGGGCGACCTACTACAATTCATTTATCA20.11911852293031568No Hit
TAGATCTGCCGAGAACTTTCCCTTACCA20.11911852293031568No Hit
TTTGTAGAATGGAAGTTAGCGGCGCTGT20.11911852293031568No Hit
TAGTCAGTGCCTTCATATATCCCTTCATT20.11911852293031568No Hit
TCGTCGTCGTGGTCAGCCGTTACCTGT20.11911852293031568No Hit
TTCCAGGCTCCCCACTCGTCTTCTGTGTT20.11911852293031568No Hit
ATACGTCGTGTATGATGCGAGGCCTTGG20.11911852293031568No Hit
TACTCCGTTGAACCATCCGACCTGTTGT20.11911852293031568No Hit
TCTAGATCTGCCGAGAACTTTCCCTTAC20.11911852293031568No Hit
TGTTTGAGGACTCCAAGCTGAAATGCCCT20.11911852293031568No Hit
TACTCCGTTGAGCCATCCGACCTGTTGT20.11911852293031568No Hit
TAATGTTTCGTTCCTTCTGGTGACCTGAC20.11911852293031568No Hit
TATGTTTCCCGGATATTGCCCAAAGGCGC20.11911852293031568No Hit
TCATCTTCTCCACCTAGTCCCTCAAATCT20.11911852293031568No Hit
CGGATATTGCCCAAAGGCGCGGCAAA20.11911852293031568No Hit
GAACCGGAACCACAGAACTCAGAGTCACT20.11911852293031568No Hit
GACTCCACGCTGAAATGCCCTGACCCGCC20.11911852293031568No Hit
TCTATGGCTTGTGTGAACACGTTTTGACA20.11911852293031568No Hit
TGTGAAGTGAAGACCATACCCTGTACCA20.11911852293031568No Hit
TTCGACGTCATCTTCTCCACCTAGTCCCTC20.11911852293031568No Hit
TGCTTTTTCGCGAAATCGTCTCCCTTGT20.11911852293031568No Hit
TCCGACCTGTTGTATCGACACCCGGCTGG20.11911852293031568No Hit
AAGCACAGGATAGAACTCAATGGAGA20.11911852293031568No Hit
GACTCCACGCTGAAATGCCCTGACCCGCCT20.11911852293031568No Hit
TCCGGTTCAGACGATGAACAATTTCCTGGC20.11911852293031568No Hit
TCCACGTTTGGTACAAAGGCTCAGCTTT20.11911852293031568No Hit
TAAACTTTCCACGAGTGCCACATAATCCCT20.11911852293031568No Hit
TGAAGGTACACAGCCTGTCGCCGACC20.11911852293031568No Hit
TGAGGACTCCACGCTGAAATGCCCTGACC20.11911852293031568No Hit
CAAACAGTGACTCGGAGTTCTGTGGTTCC20.11911852293031568No Hit
TACGGCGGTAACAATGTCCCAGAGGCTGCT20.11911852293031568No Hit
TCCTGGCCTATCTGTTTCGTGTGGACGCCT20.11911852293031568No Hit
TTCGTATCTGTCCCCAAGCTTCTCTTTGAC20.11911852293031568No Hit
TGCCGAGAACTTTCCCTTACCATACTTCC20.11911852293031568No Hit
TGTTTGATCCCCACCAAGTAACGTT20.11911852293031568No Hit
CCACGCTGAAATGCCCTGACCCGCCTTATC20.11911852293031568No Hit
TCTCATGGTCCAAGGAAACATACTTATTGT20.11911852293031568No Hit
TCAGGGCTTTGTTGTACACTTCGGTT20.11911852293031568No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)