FastQCFastQC Report
Thu 12 Nov 2020
S34.3452.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.3452.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1596
Sequences flagged as poor quality0
Sequence length16-30
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGCTCGGTACTGACTCGACAAGCGCC1358.458646616541353No Hit
TATTGTCGAATAACCCACCTCTTGTTGG1277.957393483709272No Hit
TTGGGCTCGGTACTGACTCGACAAGCGCCT885.513784461152882No Hit
TTAACGACCCGCTCTGTTCCATATCGATA462.882205513784461No Hit
TATTGTCGAATAACCCACCTCTTGG442.756892230576441No Hit
TATACGTCGTGTACGACGCGAGACCGCGGA392.443609022556391No Hit
TAAAAGGACCGATTCGGGCAGTGAGTGTC372.318295739348371No Hit
TTAACGACCCGCTCTGTTCCATATCGATAT322.0050125313283207No Hit
TTTGTTAACTGGCCGACTTCCCCCGCCT301.8796992481203008No Hit
TCGGTACTGACTCGACAAGCGCCTCGTAC271.6917293233082706No Hit
TGAATTGCCGTGGCTCGGAAGTTGGGCTC271.6917293233082706No Hit
TAACGACCCGCTCTGTTCCATATCGATAT261.6290726817042605No Hit
TCGGTACTGACTCGACAAGCGCCTCGTACT261.6290726817042605No Hit
TGGGCTCGGTACTGACTCGACAAGCGCCT241.5037593984962405No Hit
TGAGAAAAAGTCACCGTCGTCCTCATGAC191.1904761904761905No Hit
TAACGACCCGCTCTGTTCCATATCGATATT191.1904761904761905No Hit
AAAAGTCACCGTCGTCCTCATGACTGCCT191.1904761904761905No Hit
TATTGTCGAATAACCCACCTCTTGGT181.1278195488721803No Hit
TGGGCTCGGTACTGACTCGACAAGCGCC150.9398496240601504No Hit
TAACACAAGCCCGATCGCTCCGATACATG140.8771929824561403No Hit
TGGGGATCCCGTAAGAAATACACGTCGTGT130.8145363408521302No Hit
TTGGGGATCCCGTAAGAAATACACGTCGTG130.8145363408521302No Hit
TTAGATGGACCCGGTATAGGATTTTCGCTC120.7518796992481203No Hit
TCCCTCTTCGAACGTAACACAAGCCCGATC110.6892230576441103No Hit
TAACGACCCGCTCTGTTCCATATCGATA100.6265664160401002No Hit
TCCCAAACTTCGACTCGGACCAGTGCCGG100.6265664160401002No Hit
TGCCGAAGCCATAAAAGGACCGATTCGGGC90.5639097744360901No Hit
TTCGACTGTTAGGTGGGCAAAATTTGGGA90.5639097744360901No Hit
TTCTTGTTCGGAAAAGTCGCTGGCTTCGT80.5012531328320802No Hit
TCGTGTACGACGCGAGACCGCGGAGGCA80.5012531328320802No Hit
TATAGACGTTAACGGCGCTGAGGGGTGT80.5012531328320802No Hit
TTAGATGGACCCGGAATAGGATTTTCGCTC70.43859649122807015No Hit
TCACCGTCGTCCTCATGACTGCCTCCTGCC70.43859649122807015No Hit
TGTCTTTTGTTAACTGGCCGACTTCCCCC70.43859649122807015No Hit
TGAATTGCCGTGGCTCGGAAGTTGGGCTCG70.43859649122807015No Hit
TCTTTTGTTAACTGGCCGACTTCCCCCGCC60.37593984962406013No Hit
TCATCTTCTTGTTCGGAAAAGTCGCTGGCT60.37593984962406013No Hit
TTCGAAGGGTTTCGAGAAATTGGGACACT60.37593984962406013No Hit
TGACTGCCTCCTGCCACCAATCCTTCTTGT60.37593984962406013No Hit
TACTTGGATTTGTGGGATGATGGCATCGC60.37593984962406013No Hit
TACAATTTCTGCATCGTTATAGCACTGAT60.37593984962406013No Hit
TCCGATACATGGATTGAACTTCCTGAGTGT60.37593984962406013No Hit
TTATTAATGAGACCGCGGAGGACTATCTC60.37593984962406013No Hit
TAGATGGACCCGGAATAGGATTTTCGCTC60.37593984962406013No Hit
TATTGTCGAATAACCCACCTCTTGGTG50.3132832080200501No Hit
TGGGGATCCCGTAAGAAATACACGTCGTG50.3132832080200501No Hit
TGAGGACCAGGGGCTGTGTGAGGGCCTAA50.3132832080200501No Hit
TGCAATATAGACGTTAACGGCGCTGAGGGG50.3132832080200501No Hit
TTAACGACCCGCTCTGTTCCATATCGA50.3132832080200501No Hit
TTGGGCTCGGTACTGACTCGACAAGCG50.3132832080200501No Hit
AACACAAGCCCGATCGCTCCGATACATGG50.3132832080200501No Hit
TCCTGAGTGTCCGGAAGATCCTTCTGACG50.3132832080200501No Hit
CTTGGATCGGAAATGTTCATGGGGACGTT50.3132832080200501No Hit
TATTCACTGGCTTCCGCTTCAAAACTCCT50.3132832080200501No Hit
TCTTTTGTTAACTGGCCGACTTCCCCCGC50.3132832080200501No Hit
TTTGAACGTACATAACTTTTCTCCGACCCT50.3132832080200501No Hit
TTAACGACCCGCTCTGTTCCATATCGAT50.3132832080200501No Hit
TGAGAAAAAGTCACCGTCGTCCTCATGACT50.3132832080200501No Hit
TCGGATCCTATTGCATATGCGGATGCATC50.3132832080200501No Hit
TCTTCTTGTTCGGAAAAGTCGCTGGCTTCG40.2506265664160401No Hit
TACTGACTCGACAAGCGCCTCGTACTTGT40.2506265664160401No Hit
GGATGAAGACGCAGCGGCATGTTGAACTG40.2506265664160401No Hit
GATGTTGGGAAGGGGATACCTATCGCCT40.2506265664160401No Hit
TCACCGTCGTCCTCATGACTGCCTCCTGC40.2506265664160401No Hit
TGGCTTCGTATTCACTGGCTTCCGCTTCA40.2506265664160401No Hit
TCGACTGTTAGGTGGGCAAAATTTGGGA40.2506265664160401No Hit
CACAAGCCCGATCGCTCCGATACATGGATT40.2506265664160401No Hit
ATTGTCGAATAACCCACCTCTTGTTGG40.2506265664160401No Hit
TAGGGAATTTTTGAATGGCTGCTATTTT40.2506265664160401No Hit
TTCTACTTGGATTTGTGGGATGATGGCATC40.2506265664160401No Hit
TCTGAGAAAAAGTCACCGTCGTCCTCATG40.2506265664160401No Hit
TGAGGACCAGGGGCTGTGTGAGGGCCTA40.2506265664160401No Hit
TGCAATATAGACGTTAACGGCGCTGAGGG30.18796992481203006No Hit
ACCGCGGAGGCACTCTTTGAAGCAGT30.18796992481203006No Hit
TGTCTTTTGTTAACTGGCCGACTTCCCCT30.18796992481203006No Hit
TTAGACAGAATAATAACAGGAGTACGGCCC30.18796992481203006No Hit
TGTCGATTAACGAGCTCCACACTTCTTTGG30.18796992481203006No Hit
TTGGATCGGAAATGTTCATGGGGACGTT30.18796992481203006No Hit
TGTTAACTGGCCGACTTCCCCCGCCT30.18796992481203006No Hit
TCTGAGTATGGGGCTATTTCCGTGTGGAAA30.18796992481203006No Hit
TTTTTTTGGGCGGATTCTTTCGGGGGT30.18796992481203006No Hit
TACAACTCTGCCATAGACACGTCCATTAGT30.18796992481203006No Hit
TCACAATTTCCAACGTCTCGTTCCATCGGA30.18796992481203006No Hit
TCAGTGTTGAAGGCTGTTTTTGGAATTGA30.18796992481203006No Hit
CACATGAGTTGAATTGCCGTGGCTCGGAA30.18796992481203006No Hit
TCGGTACTGACTCGACAAGCGCCTCGTA30.18796992481203006No Hit
TCGTGGATGACGTGGACGTGGTCGTTGTG30.18796992481203006No Hit
TAGACGTTAACGGCGCTGAGGGGTGTTG30.18796992481203006No Hit
TCTTCTTGTTCGGAAAAGTCGCTGGCTTC30.18796992481203006No Hit
CTTCTTGTTCGGAAAAGTCGC30.18796992481203006No Hit
CGCGAGACCGCGGAGGCACTCTTTGAAGG30.18796992481203006No Hit
TGGCAGGAGATTGAACGTTTGTTTCGGTG30.18796992481203006No Hit
CACAGACGCGGTGTTTACTCTCTG30.18796992481203006No Hit
GACCGCGGAGGCACTCTTTGAAGG30.18796992481203006No Hit
TCAAAACTCCCTCTTCGAACGTAACACAAG30.18796992481203006No Hit
CACAAGCCCGATCGCTCCGATACATGGAT30.18796992481203006No Hit
TCACACACACTACTGATACTATCGGTCTCA30.18796992481203006No Hit
TTTTGTTAACTGGCCGACTTCCCCCGCCT30.18796992481203006No Hit
TGGATCGGAAATATTCATGGGGACGTTT30.18796992481203006No Hit
TCCTCATGACTGCCTCCTGCCACCAATCC30.18796992481203006No Hit
TGCATGAAGGAAGATTGATGGCAGGAGAT30.18796992481203006No Hit
TATTGTCGAATAACCCACCTCTTGTTG30.18796992481203006No Hit
TTCGAAGGGTTTCGAGAAATTGGGACA30.18796992481203006No Hit
CCGTCGTCCTCATGACTGCCTCCTGCCACC30.18796992481203006No Hit
TTGTACAATTTCTGCATCGTTATAGCACT30.18796992481203006No Hit
TGCCACGACGCATTTTCGTAGTTCTCCCCA30.18796992481203006No Hit
ATTGTCGAATAACCCACCTCTTGG30.18796992481203006No Hit
TACTGACTCGACAAGCGCCTCGTACTTG30.18796992481203006No Hit
TGTCTTTTGTTAACTGGCCGACTTCCCCCG30.18796992481203006No Hit
TTCGAGAAATTGGGACACTGTAATATTGGG30.18796992481203006No Hit
TGCAGTCGTGGATGACGTGGACGTGGTCGT30.18796992481203006No Hit
TGTGTCAGTGTTACTAATGATTCCTTCTTC30.18796992481203006No Hit
TTCCAACGTCTCGTTCCATCGGATCACTT30.18796992481203006No Hit
TCTTGGATCGGAAATGTTCATGGGGACGTT30.18796992481203006No Hit
TGGCCGACTTCCCCCGCCTGAAAGTGGTC30.18796992481203006No Hit
TCCTTCTGACGAATTCGAATCTGCGTTTC20.12531328320802004No Hit
TAACAGGAGTACGGCCCACAGTAGCGTCGC20.12531328320802004No Hit
TGTTGGTTCTGCTTGCGGCTCATTCCAAA20.12531328320802004No Hit
GACCGCGGAGGCACTCTTTGAAGCAGT20.12531328320802004No Hit
TCGCAAGTGTTTAAGATGTTCTTCTAGGG20.12531328320802004No Hit
TCGATTAACGAACTCCACACTTCTTTGGG20.12531328320802004No Hit
TTCACTGGCTTCCGCTTCAAAACTCCTCT20.12531328320802004No Hit
TCTGCATCGTTATAGCACTGATAGAATAGT20.12531328320802004No Hit
TGAGAAAAAGTCACCGTCGTCCTCATGA20.12531328320802004No Hit
TTGAATTGCCGTGGCTCGGAAGTTGGGCTC20.12531328320802004No Hit
TGATTTCATCTGTGTTTGTGTTCGTGG20.12531328320802004No Hit
TCGTACTTGTCGATTAACGAGCTCCACACT20.12531328320802004No Hit
ATTTTCGTAGTTCTCCCCAAGTTTTTGTTT20.12531328320802004No Hit
TAACTGCAGTCGTGGATGACGTGGACGT20.12531328320802004No Hit
TCGTGGATGACGTGGACGTGGTCGTTGT20.12531328320802004No Hit
TTTACGGTAGTAGCCTAGTAATCCGAGAAA20.12531328320802004No Hit
TCTGCATCGTTATAGCACTGATAGAATAG20.12531328320802004No Hit
TGGTGTAACTGCAGTCGTGGATGACGTGG20.12531328320802004No Hit
TCGTATTCACTGGCTTCCGCTTCAAAACT20.12531328320802004No Hit
TCACTGGCTTCCGCTTCAAAACTCCTCTCG20.12531328320802004No Hit
TTCGAGAAATTGGGACACTGTAATATTGG20.12531328320802004No Hit
TTTACGGTAGTAGCCTAGTAATCCGAGAA20.12531328320802004No Hit
TCTTGCTTTCGACGCGCTAGAGTGGCTGC20.12531328320802004No Hit
TGCGCCGGATGAAGACGCAGCGGCATGTTG20.12531328320802004No Hit
TAGGGAATTTTTGAATGGCTGCTATTT20.12531328320802004No Hit
TGGGCTCGGTACTGACTCGACAAGCG20.12531328320802004No Hit
TAGATGGACCCGGTATAGGATTTTCGCTC20.12531328320802004No Hit
TACTGACTCGACAAGCGCCTCGTACT20.12531328320802004No Hit
TTGGGCGGATTCTTTCGGGGGTGACA20.12531328320802004No Hit
CAATATAGACGTTAACGGCGCTGAGGGG20.12531328320802004No Hit
TTCTTGTTCGGAAAAGTCGCTGGCTTCGTA20.12531328320802004No Hit
TATGCGCCGGATGAAGACGCAGCGGCATG20.12531328320802004No Hit
TCATGACTGCCTCCTGCCACCAATCCT20.12531328320802004No Hit
TCGTTCCATCGGATCACTTGGCGTTTGAGT20.12531328320802004No Hit
TGAACGTTTGTTTCGGTGTCCACACGAGAA20.12531328320802004No Hit
CTTGTTGTAGACCTCTGTCGCACGGCCC20.12531328320802004No Hit
TATAGACGTTAACGGCGCTGAGGGGTGTT20.12531328320802004No Hit
TGGGGATCCCGTAAGAAATACAC20.12531328320802004No Hit
CACGACGCATTTTCGTAGTTCTCCCCAAG20.12531328320802004No Hit
TCTAACAAACTGACGGACACAAACCCTGAA20.12531328320802004No Hit
TCGTACAACTCTGCCATAGACACGTCCATT20.12531328320802004No Hit
TAGACGTTAACGGCGCTGAGGGGTGT20.12531328320802004No Hit
TATTGTCGAATAACCCACCTCTTGTT20.12531328320802004No Hit
TCATGACTGCCTCCTGCCACCAATCCTTC20.12531328320802004No Hit
CTTGCGGCTCATTCCAAAGAAGAATTCTT20.12531328320802004No Hit
TATTAATGAGACCGCGGAGGACTATCTC20.12531328320802004No Hit
ACGTGGTCGTTGTGGAGGCACATGAGTTT20.12531328320802004No Hit
TCTACTTGGATTTGTGGGATGATGGCATC20.12531328320802004No Hit
TAGAGTGGCTGCATGAAGGAAGGTT20.12531328320802004No Hit
TCAATCTTTTTGTGAATTCGACTGTTAGGT20.12531328320802004No Hit
TTCTAACAAACTGACGGACACAAACCCTG20.12531328320802004No Hit
TTGGGCGGATTCTTTCGGGGGTGACAATAT20.12531328320802004No Hit
TGCATGAAGGAAGATTGATGGCAGGAGATT20.12531328320802004No Hit
TGCATCGGTAGTTAATTCGAAGGGTTTCGA20.12531328320802004No Hit
TCAGTGATTTCTGAAGGGATGTGATGGGT20.12531328320802004No Hit
TGTATCTTGGATCGGAAATGTTCATGGGG20.12531328320802004No Hit
TAACAGGAGTACGGCCCACAGTAGCGTC20.12531328320802004No Hit
TGCTTTCGACGCGCTAGAGTGGCTGCAT20.12531328320802004No Hit
CCCCCGCCTGAAAGTGGTCCATTAAGGCCT20.12531328320802004No Hit
TTAACGACCCGCTCTGTTCCATATCGATC20.12531328320802004No Hit
TTGAATTGCCGTGGCTCGGAAGTTGGGC20.12531328320802004No Hit
CTGGCCGACTTCCCCCGCCTGAAAGTGGTC20.12531328320802004No Hit
TCTGCCATAGACACGTCCATTAGTTCAAGC20.12531328320802004No Hit
TTTGTGAATTCGACTGTTAGGTGGGCAA20.12531328320802004No Hit
TGCCTCCTGCCACCAATCCTTCTTGTTT20.12531328320802004No Hit
CACGACGCATTTTCGTAGTTCTCCCCAAGC20.12531328320802004No Hit
TTCGGTAGGGTCTATGTGCGAGTCACGAA20.12531328320802004No Hit
TCCTCATGACTGCCTCCTGCCACCAATCCT20.12531328320802004No Hit
TTATTAATGAGACCGCGGAGGACTATC20.12531328320802004No Hit
TCTTGTTCGGAAAAGTCGCTGGCTTCGTA20.12531328320802004No Hit
TCTGTTGTTGGGAATTCCATTTCTGAATTA20.12531328320802004No Hit
TAACGACCCGCTCTGTTCC20.12531328320802004No Hit
TCCTCTCGATTTGCGGGGAGGAGGACT20.12531328320802004No Hit
TCGGTAGGGTCTATGTGCGAGTCACGAAT20.12531328320802004No Hit
TCGAAGGGTTTCGAGAAATTGGGACACT20.12531328320802004No Hit
TAACACAAGCCCGATCGCTCCGATACATGG20.12531328320802004No Hit
TGGACGTGGTCGTTGTGGAGGCACATGAGT20.12531328320802004No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)