FastQCFastQC Report
Thu 12 Nov 2020
S34.3452.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.3452.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1781
Sequences flagged as poor quality0
Sequence length15-30
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGCTCGGTACTGACTCGACAAGCGCC1357.580011229646266No Hit
TATTGTCGAATAACCCACCTCTTGTTGG1277.130825379000562No Hit
TTGGGCTCGGTACTGACTCGACAAGCGCCT884.941044357102752No Hit
TCTATATTGTCGAATAACCCACCTCTTGG543.0320044918585065No Hit
TTAACGACCCGCTCTGTTCCATATCGATA462.582818641212802No Hit
TATTGTCGAATAACCCACCTCTTGG442.470522178551376No Hit
TATACGTCGTGTACGACGCGAGACCGCGGA392.18978102189781No Hit
TAAAAGGACCGATTCGGGCAGTGAGTGTC372.0774845592363844No Hit
TTAACGACCCGCTCTGTTCCATATCGATAT321.7967434025828188No Hit
TTTGTTAACTGGCCGACTTCCCCCGCCT301.6844469399213924No Hit
TGAATTGCCGTGGCTCGGAAGTTGGGCTC271.5160022459292533No Hit
TCGGTACTGACTCGACAAGCGCCTCGTAC271.5160022459292533No Hit
CACGTCGTGTACGACGCGAGACCGCGGAG261.4598540145985401No Hit
TAACGACCCGCTCTGTTCCATATCGATAT261.4598540145985401No Hit
TCGGTACTGACTCGACAAGCGCCTCGTACT261.4598540145985401No Hit
TGGGCTCGGTACTGACTCGACAAGCGCCT241.347557551937114No Hit
TGAGAAAAAGTCACCGTCGTCCTCATGAC191.0668163952835485No Hit
AAAAGTCACCGTCGTCCTCATGACTGCCT191.0668163952835485No Hit
TAACGACCCGCTCTGTTCCATATCGATATT191.0668163952835485No Hit
TATTGTCGAATAACCCACCTCTTGGT181.0106681639528354No Hit
TGGGCTCGGTACTGACTCGACAAGCGCC150.8422234699606962No Hit
TGAATTGCGGTGGCTCGGAAGTTGGGCTC140.7860752386299831No Hit
TAACACAAGCCCGATCGCTCCGATACATG140.7860752386299831No Hit
TGGGGATCCCGTAAGAAATACACGTCGTGT130.7299270072992701No Hit
TTGGGGATCCCGTAAGAAATACACGTCGTG130.7299270072992701No Hit
TTAGATGGACCCGGTATAGGATTTTCGCTC120.673778775968557No Hit
TATATTGTCGAATAACCCACCTCTTGG110.617630544637844No Hit
TCCCTCTTCGAACGTAACACAAGCCCGATC110.617630544637844No Hit
TAACGACCCGCTCTGTTCCATATCGATA100.5614823133071308No Hit
TCCCAAACTTCGACTCGGACCAGTGCCGG100.5614823133071308No Hit
CATTGTCGAATAACCCACCTCTTGG90.5053340819764177No Hit
TGCCGAAGCCATAAAAGGACCGATTCGGGC90.5053340819764177No Hit
TTCGACTGTTAGGTGGGCAAAATTTGGGA90.5053340819764177No Hit
TCGTGTACGACGCGAGACCGCGGAGGCA80.4491858506457047No Hit
TTCTTGTTCGGAAAAGTCGCTGGCTTCGT80.4491858506457047No Hit
CATTGTCGAATAACCCACCTCTTGGT80.4491858506457047No Hit
TATAGACGTTAACGGCGCTGAGGGGTGT80.4491858506457047No Hit
TCTATATTGTCGAATAACCCACCTCTTGGT80.4491858506457047No Hit
TTAGATGGACCCGGAATAGGATTTTCGCTC70.39303761931499154No Hit
CACGTCGTGTACGACGCGAGACCGCGGAGG70.39303761931499154No Hit
TGAATTGCCGTGGCTCGGAAGTTGGGCTCG70.39303761931499154No Hit
TCACCGTCGTCCTCATGACTGCCTCCTGCC70.39303761931499154No Hit
TGTCTTTTGTTAACTGGCCGACTTCCCCC70.39303761931499154No Hit
TCTTTTGTTAACTGGCCGACTTCCCCCGCC60.3368893879842785No Hit
TGACTGCCTCCTGCCACCAATCCTTCTTGT60.3368893879842785No Hit
TACTTGGATTTGTGGGATGATGGCATCGC60.3368893879842785No Hit
TACAATTTCTGCATCGTTATAGCACTGAT60.3368893879842785No Hit
TCCGATACATGGATTGAACTTCCTGAGTGT60.3368893879842785No Hit
TCATCTTCTTGTTCGGAAAAGTCGCTGGCT60.3368893879842785No Hit
TTCGAAGGGTTTCGAGAAATTGGGACACT60.3368893879842785No Hit
TTATTAATGAGACCGCGGAGGACTATCTC60.3368893879842785No Hit
TAGATGGACCCGGAATAGGATTTTCGCTC60.3368893879842785No Hit
TATTGTCGAATAACCCACCTCTTGGTG50.2807411566535654No Hit
TGAGGACCAGGGGCTGTGTGAGGGCCTAA50.2807411566535654No Hit
TTAACGACCCGCTCTGTTCCATATCGA50.2807411566535654No Hit
TTGGGCTCGGTACTGACTCGACAAGCG50.2807411566535654No Hit
CTTGGATCGGAAATGTTCATGGGGACGTT50.2807411566535654No Hit
TATTCACTGGCTTCCGCTTCAAAACTCCT50.2807411566535654No Hit
TCTTTTGTTAACTGGCCGACTTCCCCCGC50.2807411566535654No Hit
TTAACGACCCGCTCTGTTCCATATCGAT50.2807411566535654No Hit
TGGGGATCCCGTAAGAAATACACGTCGTG50.2807411566535654No Hit
TGCAATATAGACGTTAACGGCGCTGAGGGG50.2807411566535654No Hit
AACACAAGCCCGATCGCTCCGATACATGG50.2807411566535654No Hit
TCCTGAGTGTCCGGAAGATCCTTCTGACG50.2807411566535654No Hit
TTTGAACGTACATAACTTTTCTCCGACCCT50.2807411566535654No Hit
TGAGAAAAAGTCACCGTCGTCCTCATGACT50.2807411566535654No Hit
TCGGATCCTATTGCATATGCGGATGCATC50.2807411566535654No Hit
TCTTCTTGTTCGGAAAAGTCGCTGGCTTCG40.22459292532285235No Hit
TACACGTCGTGTACGACGCGAGACCGCGGA40.22459292532285235No Hit
TGGCTTCGTATTCACTGGCTTCCGCTTCA40.22459292532285235No Hit
TCGACTGTTAGGTGGGCAAAATTTGGGA40.22459292532285235No Hit
TTGGGGATCCCGTAAGAAATACACGTCGT40.22459292532285235No Hit
ATTGTCGAATAACCCACCTCTTGTTGG40.22459292532285235No Hit
TGAGGACCAGGGGCTGTGTGAGGGCCTA40.22459292532285235No Hit
TACTGACTCGACAAGCGCCTCGTACTTGT40.22459292532285235No Hit
GGATGAAGACGCAGCGGCATGTTGAACTG40.22459292532285235No Hit
GATGTTGGGAAGGGGATACCTATCGCCT40.22459292532285235No Hit
TCACCGTCGTCCTCATGACTGCCTCCTGC40.22459292532285235No Hit
CACAAGCCCGATCGCTCCGATACATGGATT40.22459292532285235No Hit
TCTACATTGTCGAATAACCCACCTCTTGGT40.22459292532285235No Hit
TAGGGAATTTTTGAATGGCTGCTATTTT40.22459292532285235No Hit
TTCTACTTGGATTTGTGGGATGATGGCATC40.22459292532285235No Hit
TCTGAGAAAAAGTCACCGTCGTCCTCATG40.22459292532285235No Hit
TGCAATATAGACGTTAACGGCGCTGAGGG30.16844469399213924No Hit
ACCGCGGAGGCACTCTTTGAAGCAGT30.16844469399213924No Hit
TTAGACAGAATAATAACAGGAGTACGGCCC30.16844469399213924No Hit
TGTCGATTAACGAGCTCCACACTTCTTTGG30.16844469399213924No Hit
TGTTAACTGGCCGACTTCCCCCGCCT30.16844469399213924No Hit
TTTTTTTGGGCGGATTCTTTCGGGGGT30.16844469399213924No Hit
TCGGTACTGACTCGACAAGCGCCTCGTA30.16844469399213924No Hit
TAGACGTTAACGGCGCTGAGGGGTGTTG30.16844469399213924No Hit
TCTTCTTGTTCGGAAAAGTCGCTGGCTTC30.16844469399213924No Hit
CTTCTTGTTCGGAAAAGTCGC30.16844469399213924No Hit
CGCGAGACCGCGGAGGCACTCTTTGAAGG30.16844469399213924No Hit
TGGCAGGAGATTGAACGTTTGTTTCGGTG30.16844469399213924No Hit
CACAGACGCGGTGTTTACTCTCTG30.16844469399213924No Hit
GACCGCGGAGGCACTCTTTGAAGG30.16844469399213924No Hit
TCAAAACTCCCTCTTCGAACGTAACACAAG30.16844469399213924No Hit
CACAAGCCCGATCGCTCCGATACATGGAT30.16844469399213924No Hit
TCACACACACTACTGATACTATCGGTCTCA30.16844469399213924No Hit
TATTGTCGAATAACCCACCTCTTGTTG30.16844469399213924No Hit
TTCGAAGGGTTTCGAGAAATTGGGACA30.16844469399213924No Hit
CCGTCGTCCTCATGACTGCCTCCTGCCACC30.16844469399213924No Hit
TGCCACGACGCATTTTCGTAGTTCTCCCCA30.16844469399213924No Hit
ATTGTCGAATAACCCACCTCTTGG30.16844469399213924No Hit
TACTGACTCGACAAGCGCCTCGTACTTG30.16844469399213924No Hit
TTCGAGAAATTGGGACACTGTAATATTGGG30.16844469399213924No Hit
TGCAGTCGTGGATGACGTGGACGTGGTCGT30.16844469399213924No Hit
TTCCAACGTCTCGTTCCATCGGATCACTT30.16844469399213924No Hit
TCTTGGATCGGAAATGTTCATGGGGACGTT30.16844469399213924No Hit
TGGCCGACTTCCCCCGCCTGAAAGTGGTC30.16844469399213924No Hit
TCTTGTGAATTCGACTGTTAGGTGGGCA30.16844469399213924No Hit
TGTCTTTTGTTAACTGGCCGACTTCCCCT30.16844469399213924No Hit
TTGGATCGGAAATGTTCATGGGGACGTT30.16844469399213924No Hit
TCTGAGTATGGGGCTATTTCCGTGTGGAAA30.16844469399213924No Hit
TACAACTCTGCCATAGACACGTCCATTAGT30.16844469399213924No Hit
TCACAATTTCCAACGTCTCGTTCCATCGGA30.16844469399213924No Hit
TCAGTGTTGAAGGCTGTTTTTGGAATTGA30.16844469399213924No Hit
CACATGAGTTGAATTGCCGTGGCTCGGAA30.16844469399213924No Hit
TCGTGGATGACGTGGACGTGGTCGTTGTG30.16844469399213924No Hit
TTTTGTTAACTGGCCGACTTCCCCCGCCT30.16844469399213924No Hit
TGGATCGGAAATATTCATGGGGACGTTT30.16844469399213924No Hit
TCCTCATGACTGCCTCCTGCCACCAATCC30.16844469399213924No Hit
TGCATGAAGGAAGATTGATGGCAGGAGAT30.16844469399213924No Hit
TTGTACAATTTCTGCATCGTTATAGCACT30.16844469399213924No Hit
TGTCTTTTGTTAACTGGCCGACTTCCCCCG30.16844469399213924No Hit
CACGTCGTGTACGACGCGAGACCG30.16844469399213924No Hit
TGTGTCAGTGTTACTAATGATTCCTTCTTC30.16844469399213924No Hit
GACCGCGGAGGCACTCTTTGAAGCAGT20.11229646266142618No Hit
TCGATTAACGAACTCCACACTTCTTTGGG20.11229646266142618No Hit
TCTCGATTGATTAAAACTGCACAGACGCG20.11229646266142618No Hit
TCGTACTTGTCGATTAACGAGCTCCACACT20.11229646266142618No Hit
TTTACGGTAGTAGCCTAGTAATCCGAGAAA20.11229646266142618No Hit
TATCTCGATTGATTAAAACTGCACAGACGC20.11229646266142618No Hit
TCGTATTCACTGGCTTCCGCTTCAAAACT20.11229646266142618No Hit
TCACTGGCTTCCGCTTCAAAACTCCTCTCG20.11229646266142618No Hit
TTCGAGAAATTGGGACACTGTAATATTGG20.11229646266142618No Hit
TTTACGGTAGTAGCCTAGTAATCCGAGAA20.11229646266142618No Hit
TCTTGCTTTCGACGCGCTAGAGTGGCTGC20.11229646266142618No Hit
TGCGCCGGATGAAGACGCAGCGGCATGTTG20.11229646266142618No Hit
TACTGACTCGACAAGCGCCTCGTACT20.11229646266142618No Hit
TTGGGCGGATTCTTTCGGGGGTGACA20.11229646266142618No Hit
CAATATAGACGTTAACGGCGCTGAGGGG20.11229646266142618No Hit
TTCTTGTTCGGAAAAGTCGCTGGCTTCGTA20.11229646266142618No Hit
TCATGACTGCCTCCTGCCACCAATCCT20.11229646266142618No Hit
TGAACGTTTGTTTCGGTGTCCACACGAGAA20.11229646266142618No Hit
CTTGTTGTAGACCTCTGTCGCACGGCCC20.11229646266142618No Hit
TATAGACGTTAACGGCGCTGAGGGGTGTT20.11229646266142618No Hit
TGGGGATCCCGTAAGAAATACAC20.11229646266142618No Hit
CACGACGCATTTTCGTAGTTCTCCCCAAG20.11229646266142618No Hit
TCTAACAAACTGACGGACACAAACCCTGAA20.11229646266142618No Hit
TAGACGTTAACGGCGCTGAGGGGTGT20.11229646266142618No Hit
TATTGTCGAATAACCCACCTCTTGTT20.11229646266142618No Hit
TCATGACTGCCTCCTGCCACCAATCCTTC20.11229646266142618No Hit
CTTGCGGCTCATTCCAAAGAAGAATTCTT20.11229646266142618No Hit
ACGTGGTCGTTGTGGAGGCACATGAGTTT20.11229646266142618No Hit
TCTACTTGGATTTGTGGGATGATGGCATC20.11229646266142618No Hit
TCCAGACTTCTTTGGGGATCCCGTAAGAAA20.11229646266142618No Hit
CATTGTCGAATAACCCACCTCTTG20.11229646266142618No Hit
TTGGGCGGATTCTTTCGGGGGTGACAATAT20.11229646266142618No Hit
TGCATGAAGGAAGATTGATGGCAGGAGATT20.11229646266142618No Hit
TGCATCGGTAGTTAATTCGAAGGGTTTCGA20.11229646266142618No Hit
TCAGTGATTTCTGAAGGGATGTGATGGGT20.11229646266142618No Hit
TGCTTTCGACGCGCTAGAGTGGCTGCAT20.11229646266142618No Hit
TTAACGACCCGCTCTGTTCCATATCGATC20.11229646266142618No Hit
TTGAATTGCCGTGGCTCGGAAGTTGGGC20.11229646266142618No Hit
CTGGCCGACTTCCCCCGCCTGAAAGTGGTC20.11229646266142618No Hit
TCTGCCATAGACACGTCCATTAGTTCAAGC20.11229646266142618No Hit
CACGTCGTGTACGACGCGAGACCGCGGA20.11229646266142618No Hit
TTCGGTAGGGTCTATGTGCGAGTCACGAA20.11229646266142618No Hit
TTATTAATGAGACCGCGGAGGACTATC20.11229646266142618No Hit
TCTTGTTCGGAAAAGTCGCTGGCTTCGTA20.11229646266142618No Hit
TCTGTTGTTGGGAATTCCATTTCTGAATTA20.11229646266142618No Hit
GATGCGAGACCGCGGAGGCACTCTTTGAAG20.11229646266142618No Hit
TCGGTAGGGTCTATGTGCGAGTCACGAAT20.11229646266142618No Hit
TCGAAGGGTTTCGAGAAATTGGGACACT20.11229646266142618No Hit
TAACACAAGCCCGATCGCTCCGATACATGG20.11229646266142618No Hit
TCCTTCTGACGAATTCGAATCTGCGTTTC20.11229646266142618No Hit
TAACAGGAGTACGGCCCACAGTAGCGTCGC20.11229646266142618No Hit
TGTTGGTTCTGCTTGCGGCTCATTCCAAA20.11229646266142618No Hit
TCGCAAGTGTTTAAGATGTTCTTCTAGGG20.11229646266142618No Hit
TTCACTGGCTTCCGCTTCAAAACTCCTCT20.11229646266142618No Hit
TCTGCATCGTTATAGCACTGATAGAATAGT20.11229646266142618No Hit
TGAGAAAAAGTCACCGTCGTCCTCATGA20.11229646266142618No Hit
TTGAATTGCCGTGGCTCGGAAGTTGGGCTC20.11229646266142618No Hit
TGATTTCATCTGTGTTTGTGTTCGTGG20.11229646266142618No Hit
ATTTTCGTAGTTCTCCCCAAGTTTTTGTTT20.11229646266142618No Hit
TAACTGCAGTCGTGGATGACGTGGACGT20.11229646266142618No Hit
TCGTGGATGACGTGGACGTGGTCGTTGT20.11229646266142618No Hit
TCTGCATCGTTATAGCACTGATAGAATAG20.11229646266142618No Hit
TGGTGTAACTGCAGTCGTGGATGACGTGG20.11229646266142618No Hit
TAGGGAATTTTTGAATGGCTGCTATTT20.11229646266142618No Hit
TGGGCTCGGTACTGACTCGACAAGCG20.11229646266142618No Hit
TAGATGGACCCGGTATAGGATTTTCGCTC20.11229646266142618No Hit
TATGCGCCGGATGAAGACGCAGCGGCATG20.11229646266142618No Hit
TCGTTCCATCGGATCACTTGGCGTTTGAGT20.11229646266142618No Hit
TCGTACAACTCTGCCATAGACACGTCCATT20.11229646266142618No Hit
TATTAATGAGACCGCGGAGGACTATCTC20.11229646266142618No Hit
TAGAGTGGCTGCATGAAGGAAGGTT20.11229646266142618No Hit
TCAATCTTTTTGTGAATTCGACTGTTAGGT20.11229646266142618No Hit
TTCTAACAAACTGACGGACACAAACCCTG20.11229646266142618No Hit
TGTATCTTGGATCGGAAATGTTCATGGGG20.11229646266142618No Hit
TAACAGGAGTACGGCCCACAGTAGCGTC20.11229646266142618No Hit
CCCCCGCCTGAAAGTGGTCCATTAAGGCCT20.11229646266142618No Hit
TTTGTGAATTCGACTGTTAGGTGGGCAA20.11229646266142618No Hit
TGCCTCCTGCCACCAATCCTTCTTGTTT20.11229646266142618No Hit
CACGACGCATTTTCGTAGTTCTCCCCAAGC20.11229646266142618No Hit
TCCTCATGACTGCCTCCTGCCACCAATCCT20.11229646266142618No Hit
CTATATTGTCGAATAACCCACCTCTTGG20.11229646266142618No Hit
ACAAGAACAACAGAA20.11229646266142618No Hit
TAACGACCCGCTCTGTTCC20.11229646266142618No Hit
TCCTCTCGATTTGCGGGGAGGAGGACT20.11229646266142618No Hit
CCGCGGAGGCACTCTTTGAAGCAGTGCCA20.11229646266142618No Hit
TGGACGTGGTCGTTGTGGAGGCACATGAGT20.11229646266142618No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)