FastQCFastQC Report
Mon 30 Nov 2020
S33.15-35nt.14987.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.14987.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4729
Sequences flagged as poor quality0
Sequence length15-35
%GC66

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGTGCCGGAACAGCTGCTGGACC851.7974201733981814No Hit
CTATCGCCTCCCGGGTGGCCAAGGATCTCCTCTG851.7974201733981814No Hit
AAACCCTGCGCGCCGTCTGTTCCTCGCACA330.6978219496722351No Hit
CTGCCCACTGGGGTGTCGCGGCC330.6978219496722351No Hit
AACCCTGCGCGCCGTCTGTTCCTCGCACA310.6555297102981603No Hit
GAAGCCGGAATGCTGAAGTGGGGGGTCTCCGAGG300.6343835906111228No Hit
CTTCCGTCTGGTCCTCCGAAGCCT270.5709452315500105No Hit
CGTAAAGCGCTGGGATCTTAAGTTTGAGATTTCTC210.444068513427786No Hit
CTTCGTCAAAGCTCCCCTGCCCCCATCCGG200.4229223937407486No Hit
CTACAACCTGGCCACCGCGGACCTCGC190.4017762740537111No Hit
GAAGCCGGAATGCTGAAGTGGGGGGTCTCCGAGGG180.38063015436667375No Hit
AGGTCGGCGTGCGTGACACCTTCCGGTTGGCC180.38063015436667375No Hit
TATCGCCTCCCGGGTGGCCAAGGATCTCCTCTG180.38063015436667375No Hit
ATCTGTCGAGACAGGACGAACGAGCGATGGCGGT170.3594840346796363No Hit
CCCACTTCGTGGTGCCGGAACAGCTGCTGGACC170.3594840346796363No Hit
TCGTGGTGCCGGAACAGCTGCTGGACC160.33833791499259885No Hit
ATCTGTCGAGACAGGACGAACGAGCGATGGCGGTG140.296045675618524No Hit
CGCACTCAAGGTGGACTCCGACCCGTGG140.296045675618524No Hit
TTCGTCAAAGCTCCCCTGCCCCCATCCGG130.27489955593148657No Hit
ATGACGAGCGACTGGGCGGCGCCCCT130.27489955593148657No Hit
TGACGAGCGACTGGGCGGCGCCCCTGGA130.27489955593148657No Hit
CTTCCGTCTGGTCCTCCGAAGCCTCTTCCCT130.27489955593148657No Hit
TTTGTACGCCGCCCCAGGAAGAGGCCCACC130.27489955593148657No Hit
GTGGTGCCGGAACAGCTGCTGGACC130.27489955593148657No Hit
CGGGGGTCGTCCGTC120.2537534362444491No Hit
ATCGATCGCCTGACGCCTTCGTCCTCGCAGA120.2537534362444491No Hit
CGGAACAGCTGCTGGACC120.2537534362444491No Hit
GGTCGGCGTGCGTGACACCTTCCGGTTGGCCGGC120.2537534362444491No Hit
CTTCGTGGTGCCGGAACAGCTGCTGGACC110.2326073165574117No Hit
CGTTTGTACGCCGCCCCAGGAAGAGGCCCACC110.2326073165574117No Hit
CCTTCGATGACGAGCGACTGGGCGGCGCCCCTGGA110.2326073165574117No Hit
AGAGGTCTCCCCTCTTCTCCGGTCTCTGTCGCGCC110.2326073165574117No Hit
CAACGGGGGTCCATGCCCGCTGGGCGCGTCCACT110.2326073165574117No Hit
TTCGTGGTGCCGGAACAGCTGCTGGACC100.2114611968703743No Hit
CCCCTGAGGGATTCGTCGGCGTGCAGG100.2114611968703743No Hit
AGTCGCTCGGGTCTGTCTCTTGCTGAG100.2114611968703743No Hit
CTGGCCACCGCGGACCTCGC100.2114611968703743No Hit
CGACGACCCAGAGCGAACCCGTGCGCGCTCGG100.2114611968703743No Hit
TGGCCACCGCGGACCTCGCCT100.2114611968703743No Hit
ATCCTTCTCGTCGTCCGCCGCTGTGGC100.2114611968703743No Hit
ATCCGTCCCTTCGATGACGAGCGACTGGGC100.2114611968703743No Hit
TTGAACCTCTACAACCTGGCCACCGCGGACCTCGC100.2114611968703743No Hit
TTCGATGACGAGCGACTGGGCGGCGCCCCTGGA90.19031507718333687No Hit
CGAGGCTGTCCCGGATCTCCG90.19031507718333687No Hit
CGTAAAGCGCTGGGATCTTAAGTTTGAGATTTCT90.19031507718333687No Hit
CCGGTGCGCGCGGCCGCGGTACCCGAGTTGGG90.19031507718333687No Hit
AAGCCGGAATGCTGAAGTGGGGGGTCTCCGAGG90.19031507718333687No Hit
ATTATTACTGGGCCCGCTTCTGGAC90.19031507718333687No Hit
CACTTCGTGGTGCCGGAACAGCTGCTGGACC90.19031507718333687No Hit
CGAAGCCGGAATGCTGAAGTGGGGGGTCTCCGAGG90.19031507718333687No Hit
TTTGTACGCCGCCCCAGGAAGAGGCCCACCC90.19031507718333687No Hit
CTTCTCCGGTCTCTGTCGCGCC90.19031507718333687No Hit
CGCCTCCCGGGTGGCCAAGGATCTCCTCTG90.19031507718333687No Hit
CAGGCGACCGCTGTCCCGATGGAGGAGG90.19031507718333687No Hit
TCCTATCGATCGCCTGACGCC90.19031507718333687No Hit
CTACAACCTGGCCACCGCGGAC90.19031507718333687No Hit
ATCGCCTCCCGGGTGGCCAAGGATCTCCTCTG80.16916895749629943No Hit
AAGGTCGGCGTGCGTGACACCTTCCGGTTGGCC80.16916895749629943No Hit
GAAACCCTGCGCGCCGTCTGTTCCTCGCACA80.16916895749629943No Hit
GAAGCCGGAATGCTGAAGTGGGGGGTCTCCGAG80.16916895749629943No Hit
GGTGCCGGAACAGCTGCTGGACC80.16916895749629943No Hit
CTACAACCTGGCCACCGCGGACCTCGCC80.16916895749629943No Hit
TTCCGGTGCGCGCGGCCGCGGTACCCGAGTTGGGC80.16916895749629943No Hit
TCTGGACCGAGACCCGAACGG80.16916895749629943No Hit
GGCCCTTTGGCGTGGTCTCTGTGACGGCCTCAAGG80.16916895749629943No Hit
ATGACGAGCGACTGGGCGGCGCCCCTGGAA80.16916895749629943No Hit
CCACTTCGTGGTGCCGGAACAGCTGCTGGACCAGT80.16916895749629943No Hit
CCCTGCGCGCCGTCTGTTCCTCGCACA80.16916895749629943No Hit
ACTTCGTGGTGCCGGAACAGCTGCTGGACCA80.16916895749629943No Hit
TCGTGGTGCCGGAACAGCTGCTGGACCA80.16916895749629943No Hit
CTTCGTCAAAGCTCCCCTGCCCCCATCCGGG80.16916895749629943No Hit
TCCCACTTCGTGGTGCCGGAACAGCTGCTGGACC80.16916895749629943No Hit
ACCTCTACAACCTGGCCACCGCGGACCTCGCC70.148022837809262No Hit
AGCCGGAATGCTGAAGTGGGGGGTCTCCGAGG70.148022837809262No Hit
CGTGGTGCCGGAACAGCTGCTGGACCA70.148022837809262No Hit
ATGACGAGCGACTGGGCGGCGCCCCTGGA70.148022837809262No Hit
CGGAGCCCCGCGTCGACCTTTCGGG70.148022837809262No Hit
AAAGCGCTGGGATCTTAAGTTTGAGATTTCTC70.148022837809262No Hit
CCCCTGAGGGATTCGTCGGCGTGCAGGCG70.148022837809262No Hit
TCCCCCTGAGGGATTCGTCGGCGTGCAGGC70.148022837809262No Hit
CCCCGTGAGACACCTCCTCCCTGGTCCGTCCGACG70.148022837809262No Hit
CCGTCTGGTCCTCCGAAGCCT70.148022837809262No Hit
CGATGACGAGCGACTGGGCGGCGCCC70.148022837809262No Hit
AATATTGAGGAGGAGGAGGAGATCGCGGCGGA70.148022837809262No Hit
CTTCCGTCTGGTCCTCCGAAGCCTCTTCC70.148022837809262No Hit
CTTCGATGACGAGCGACTGGGCGGCGCCCCTGGA70.148022837809262No Hit
ATCTGTCGAGACAGGACGAACGAGCGATGGCGG70.148022837809262No Hit
GGTCGGCGTGCGTGACACCTTCCGGTTGGCC70.148022837809262No Hit
TCGGGCCGCAGAGACGTCGCC60.12687671812222456No Hit
GCGCTCGCGCTCTGGGCGCGGACCAGGCGGCG60.12687671812222456No Hit
CGTCCTCCGGAGATCTCTCGGGCTTTCGAGACC60.12687671812222456No Hit
GACGAGCGACTGGGCGGCGCCCCTGGA60.12687671812222456No Hit
CTCAAGGTGGACTCCGACCCGTGGAGACAGGG60.12687671812222456No Hit
CCTATCGATCGCCTGACGCCT60.12687671812222456No Hit
CGGGCGGACAGTGGCCCCTCTCGGCACGCA60.12687671812222456No Hit
ATGCGGATTATGCAGGGACCT60.12687671812222456No Hit
TGCCGGAACAGCTGCTGGACC60.12687671812222456No Hit
CTGAGGACCCCGCTGCTCTTCGAGCGACT60.12687671812222456No Hit
CTGGACCGAGACCCGAACGG60.12687671812222456No Hit
CGTGCGTGACACCTTCCGGTTGGCCGGCTTGG60.12687671812222456No Hit
CCTGGCCACCGCGGACCTCGCCT60.12687671812222456No Hit
CAGGACGAACGAGCGATGGCGGTGGGTATCCT60.12687671812222456No Hit
CATCGGCTTCCGGTGCGCGCGGCCGCGGTACC60.12687671812222456No Hit
GTTTGTACGCCGCCCCAGGAAGAGGCCCACC60.12687671812222456No Hit
TATTATTACTGGGCCCGCTTCTGGAC60.12687671812222456No Hit
CCCTGAGGGATTCGTCGGCGTGCAGG60.12687671812222456No Hit
ACTTCGTGGTGCCGGAACAGCTGCTGGACC60.12687671812222456No Hit
CTTCCGGTGCGCGCGGCCGCGGTACCCGAGTTGGG60.12687671812222456No Hit
ACATTCGGTTCCCGAAGAGTCGCACT60.12687671812222456No Hit
ATATTGAGGAGGAGGAGGAGATCGCGGCGGA60.12687671812222456No Hit
CTGGCCACCGCGGACCTCGCC60.12687671812222456No Hit
AAATATTGAGGAGGAGGAGGAGATCGCGGCGG60.12687671812222456No Hit
CCGTCCTCCGGAGATCTCTCGGGCTTTCGAGACC60.12687671812222456No Hit
CCTGACTGCCGCCACAGACCTCATGCCTACGGAC60.12687671812222456No Hit
AACCCTGCGCGCCGTCTGTTCCTC60.12687671812222456No Hit
ACCACTCGTGCTCAGAGGGGCTGTATGATGGGC60.12687671812222456No Hit
CTATCGCCTCCCGGGTGGCCAAGGATCTCCT50.10573059843518715No Hit
AGCCGGAATGCTGAAGTGGGGGGTCTCCGAG50.10573059843518715No Hit
CCGAACAGTCGCCGCGGAGGCACGCGCCGAGGGC50.10573059843518715No Hit
ATCGATCGCCTGACGCCTTCGTCCTCGCAGAGA50.10573059843518715No Hit
AAACCCTGCGCGCCGTCTGTTCCTC50.10573059843518715No Hit
CAGGACGAACGAGCGATGGCGGTGGGTATCCTTCG50.10573059843518715No Hit
ATACGAAGAGCGCTCGCCAAG50.10573059843518715No Hit
GTTACGGCTCCGCCTGCCCACTGGGGTGTCGCGGC50.10573059843518715No Hit
ATCGGCTTCCGGTGCGCGCGGCCGCGGTACCCGA50.10573059843518715No Hit
GTGGTGCCGGAACAGCTGCTGGACCA50.10573059843518715No Hit
CTTCGAGCGACTGGTCGGGCGGACAGTGGC50.10573059843518715No Hit
CTACAACCTGGCCACCGCGGACCTCG50.10573059843518715No Hit
ATGACCTGGCCGCAGCCTTCTCGCCCGAGC50.10573059843518715No Hit
CGCACTCAAGGTGGACTCCGACCCGTGGA50.10573059843518715No Hit
CTCCGGCGGGTATGGGACTCCTTCC50.10573059843518715No Hit
GCGACGACCCAGAGCGAACCCGTGCGCGCTCGGA50.10573059843518715No Hit
CTTCGTGGTGCCGGAACAGCTGCTGGACCA50.10573059843518715No Hit
CTGAGGGATTCGTCGGCGTGCAGGC50.10573059843518715No Hit
ATGCTGAAGTGGGGGGTCTCCGAGG50.10573059843518715No Hit
CTCTACAACCTGGCCACCGCGGACCTCGCC50.10573059843518715No Hit
CGCCGACCTGACTGCCGCCACAGACCTCATGCCT50.10573059843518715No Hit
CTGCGCGCCGTCTGTTCCTCGCACA50.10573059843518715No Hit
GACCACTCGTGCTCAGAGGGGCTGTATGATGGGC50.10573059843518715No Hit
CTTCCGTCTGGTCCTCCGAAGCCTCTTCCCTCGCT50.10573059843518715No Hit
CTTCGATGACGAGCGACTGGGCGGCGCCC50.10573059843518715No Hit
GAGGACCCCGCTGCTCTTCGAGCGACT50.10573059843518715No Hit
GGAGCCCCGCGTCGACCTTTCGGGC50.10573059843518715No Hit
CGACGAAGGGCTGCCCGCTTACGCGGTC50.10573059843518715No Hit
CTCTACAACCTGGCCACCGCGGACCTCGC50.10573059843518715No Hit
CCTGCGCGCCGTCTGTTCCTCGCACA50.10573059843518715No Hit
CGAGGATCCTGGGGCACGTGGGGGGGATGGG50.10573059843518715No Hit
CTCGGGTCTGTCTCTTGCTGAGGTAGC50.10573059843518715No Hit
CGGACAGTGGCCCCTCTCGGCACGCACGTGGA50.10573059843518715No Hit
CCCACTTCGTGGTGCCGGAACAGCTGCTGGACCA50.10573059843518715No Hit
AAGAGGCCACCATCGAAGGGC50.10573059843518715No Hit
TGGCCACCGCGGACCTCGC50.10573059843518715No Hit
CGGAGCCCCGCGTCGACCTTTCGGGC50.10573059843518715No Hit
GGGGTCATGGCTGAACCACGACTTGGTAGAACGGG50.10573059843518715No Hit
CGGCACGCACGTGGAGGTCGTAGG50.10573059843518715No Hit
GTTTGTACGCCGCCCCAGGAAGAGGCCCACCC50.10573059843518715No Hit
TGGCCACCGCGGACCTCGCCTGTGTCGACGGGG50.10573059843518715No Hit
CTACAACCTGGCCACCGCGGACCTC50.10573059843518715No Hit
CTCGCGCTCTGGGCGCGGACCAGGCGGCG50.10573059843518715No Hit
CTTCGATGACGAGCGACTGGGCGGCGCCCCT50.10573059843518715No Hit
CGCACTCAAGGTGGACTCCGACCCGTG50.10573059843518715No Hit
AAATATTGAGGAGGAGGAGGAGATCGCGGCGGA50.10573059843518715No Hit
TTCCGTCTGGTCCTCCGAAGCCT50.10573059843518715No Hit
CGACCTGACTGCCGCCACAGACCTCATGC50.10573059843518715No Hit
CGCCTGCAGGGCGGACAACGGAAGGCGCT50.10573059843518715No Hit
AACCGCGCGAAGTCCTATCGATCGCCTGACG50.10573059843518715No Hit
AGCCGGAATGCTGAAGTGGGGGGTCTCCGAGGG50.10573059843518715No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)