FastQCFastQC Report
Mon 30 Nov 2020
S33.15-35nt.14987.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.14987.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430
Sequences flagged as poor quality0
Sequence length15-35
%GC66

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGAGATCTCCGGAGGACGG327.441860465116279No Hit
TCCCCGGAGACTCCTGACGAC173.953488372093023No Hit
CGAGAAGGATCCAAGGACGGA122.7906976744186047No Hit
GGAGATCGCGGCACTCGGCCT122.7906976744186047No Hit
TGCTTGGCGAGCGCTCTTCGT61.3953488372093024No Hit
ATCCGGCGACGTCTCTGCGGC61.3953488372093024No Hit
TTCCCCGGAGACTCCTGACGA61.3953488372093024No Hit
ACGAGAAGGATCCAAGGACGG51.1627906976744187No Hit
GAGTACATGACCCCACCGGCG51.1627906976744187No Hit
TCCTCGATCGCCTGCACGCCG40.9302325581395349No Hit
GCACATCCGCCATCGCCGGGG40.9302325581395349No Hit
AGCACGAGTGGTCAGAGGGCC40.9302325581395349No Hit
TCCGGCGACGTCTCTGCGGCC40.9302325581395349No Hit
AGGATACCCACCGCCATCGCT40.9302325581395349No Hit
TCATCCAGCTCAGGACGCGCC40.9302325581395349No Hit
TCGGGCGAGAAGGCTGCGGCC40.9302325581395349No Hit
CATGGAACCTCGCAGCGGATC30.6976744186046512No Hit
GAACCTCGCAGCGGATCCGGC30.6976744186046512No Hit
GCGGGCAGCCCTTCGTCGGACC30.6976744186046512No Hit
TCGGGGCATAGCGAGCACGGG30.6976744186046512No Hit
TCCGGGTAAACGCAGTCGACT30.6976744186046512No Hit
CTTCGTCGGACGGACCAGGGA30.6976744186046512No Hit
GGATTACTCCCCCGTCGACAC30.6976744186046512No Hit
AGGCTTCGGAGGACCAGACGG30.6976744186046512No Hit
AACCTCGCAGCGGATCCGGCG30.6976744186046512No Hit
TCTGACGGGCACGGGGGGGG20.46511627906976744No Hit
CAGCAGCGCCGCCTGGTCCGC20.46511627906976744No Hit
GAAAGGTCGACGCGGGGCTCC20.46511627906976744No Hit
AAGGATCCAAGGACGGACGCC20.46511627906976744No Hit
TACGGGTCTCGGAAGGGCGGG20.46511627906976744No Hit
CCCCAGGATCCTCGATCGCCT20.46511627906976744No Hit
AAGACGTTCGAGCTCCTCGGA20.46511627906976744No Hit
GCGAGGGAAGAGGCTTCGGAGGACCAGA20.46511627906976744No Hit
GTCCCGCTGCTATCCACCGAA20.46511627906976744No Hit
CCGGATTGCGTCGGCCGCGAC20.46511627906976744No Hit
CCGGAGATCGCGGCACTCGGC20.46511627906976744No Hit
GACGAGAAGGATCCAAGGACG20.46511627906976744No Hit
AGGGCCCGCGGATCACGAGC20.46511627906976744No Hit
CCTTCGATGGTGGCCTCTTCC20.46511627906976744No Hit
ATGAGGTCTGTGGCGGCAGTC20.46511627906976744No Hit
CTCGGCCTGAGTGGACGCGCCT20.46511627906976744No Hit
CACGAGCGAGGGTCGGGGCGT20.46511627906976744No Hit
GCATCGGGATCCTCGTCGACG20.46511627906976744No Hit
CCCACGTGCCCCAGGATCCTC20.46511627906976744No Hit
AGAGGCTTCGGAGGACCAGACCA20.46511627906976744No Hit
CACCCGGATGGGGGCAGGGGAT20.46511627906976744No Hit
CGGACGACGAGAAGGATCCAAGGAC20.46511627906976744No Hit
TCAGCAAGAGACAGACCCGAGCGCCT20.46511627906976744No Hit
AACAGACGGCGCGCAGGGTTT20.46511627906976744No Hit
CGGACGGACCAGGGAGGAGGTC20.46511627906976744No Hit
TCGGTCCCGCTGCTATCCACC20.46511627906976744No Hit
GCTGGTAAACCGTCTCGGAGT20.46511627906976744No Hit
AGGACTTCGCGCGGTTCGCCT20.46511627906976744No Hit
AGTGAAGAGCGGCCGCGCTGC20.46511627906976744No Hit
ACATCGGCCACCACCTGGAGG20.46511627906976744No Hit
AGTCAGGTCGGCGGAGTACATGAC20.46511627906976744No Hit
GACGAGAAGGATCCAAGGAC20.46511627906976744No Hit
AGGGACGGATTCTCCGGGGGTC20.46511627906976744No Hit
GGTCAAGGGGCGCTGCTCTCG20.46511627906976744No Hit
TCACCCGGATGGGGGCAGGG20.46511627906976744No Hit
TCGGCCTGAGTGGACGCGCCC20.46511627906976744No Hit
GGCATGAGGTCTGTGGCGGCA20.46511627906976744No Hit
GGGATCCTCGTCGACGGTGAATTCGCCCCGGT10.23255813953488372No Hit
TCTCGACAGATCGAGACCGGACTCCAAGC10.23255813953488372No Hit
CCAGAGGAGATCCTTGGCCACCA10.23255813953488372No Hit
CCTTGAGGCCGTCACAGAGAC10.23255813953488372No Hit
CGTCGGACGGACCAGGGAGGA10.23255813953488372No Hit
CATCCTCCTCCTCCT10.23255813953488372No Hit
TTCGAGAAGCTCCGAGCGCGC10.23255813953488372No Hit
AGGACTTCGCGCGGTTCGCC10.23255813953488372No Hit
ACGTCTCATTCCTCCCAAGCT10.23255813953488372No Hit
TGTCTCCGCTCGTCGTGCCCC10.23255813953488372No Hit
CGAAACTCCCCATCACCCC10.23255813953488372No Hit
TGGTCATGGAACCTCGCAGCGGATCCGG10.23255813953488372No Hit
ACTCCCCCGTCGACACAGGCG10.23255813953488372No Hit
CCGCATCGGGATCCTCGTC10.23255813953488372No Hit
CCAAGAGTGTATCCCGCC10.23255813953488372No Hit
CTTCGGAGGACCAGACGGAAGGA10.23255813953488372No Hit
GAGATGGTCGTCTTCGAAGCCAAGAGTGTATC10.23255813953488372No Hit
CGGCCAAATGGCCTTCCGGACC10.23255813953488372No Hit
GTCCCATACCCGCCGGAGATCGCGGCACT10.23255813953488372No Hit
AGCTGAAGCGCTGTAAGGGCC10.23255813953488372No Hit
CGACGAGAAGGATCCAAGGACGGACGCCCGA10.23255813953488372No Hit
CCACGAGACACCTCCCCCAGC10.23255813953488372No Hit
CTCCGCTCGTCGTGCCCCTCG10.23255813953488372No Hit
GCTCGGGCGAGAAGGCTGCGGCCAGGTCATCCC10.23255813953488372No Hit
TCCGTATACCAGGTTCCCTACCCTCCCGAAA10.23255813953488372No Hit
AGGGGGAACCCACGGGGGACC10.23255813953488372No Hit
ATCACGAGCGAGGGTCGGGGC10.23255813953488372No Hit
GCCCAGTCGCTCGTCATCGACC10.23255813953488372No Hit
CCGGAGATCGCGGCACTCGGCC10.23255813953488372No Hit
CCTCGTCGACGGTGAATTCGC10.23255813953488372No Hit
GGATCCTCGATCGCCTGCACG10.23255813953488372No Hit
ACGAGAAGGATCCAAGGACG10.23255813953488372No Hit
TCCCCGGAGACTCCTGACGA10.23255813953488372No Hit
AGGAACAGACGGCGCGCAGGG10.23255813953488372No Hit
AGCGGATCCGGCGACGTCTCTGCGGCCC10.23255813953488372No Hit
ACTCGGCCTGAGTGGACGCGCCCT10.23255813953488372No Hit
CGAGTGGTCAGAGGGCCGTCT10.23255813953488372No Hit
CACCAACAGGGCCAGGGGGAG10.23255813953488372No Hit
CCGAAAGGTCGACGCGGGGC10.23255813953488372No Hit
CAGCTGAAGCGCTGTAAGGGC10.23255813953488372No Hit
TCCGAAGACGTTCGAGCTCCTT10.23255813953488372No Hit
CAGCCGGAGGCATCGAACGAC10.23255813953488372No Hit
AGATGACCCAAGACAGATTG10.23255813953488372No Hit
TACCAGGTTCCCTACCCTCCC10.23255813953488372No Hit
CGCCCAGGCACCTTGGAAGAA10.23255813953488372No Hit
TGACGGGCACGGGGGGGGGAGTA10.23255813953488372No Hit
AGCCGTAACGACTCTCGCCTTATTGCCCAACT10.23255813953488372No Hit
CAGCTCAGGACGCGCCAAATCC10.23255813953488372No Hit
CACCCGGATGGGGGCAGGGGA10.23255813953488372No Hit
GGGGCCCCCTTCCGAAGAC10.23255813953488372No Hit
CTGCTATCCACCGAACCATCCGGATCCCGCC10.23255813953488372No Hit
GACCGAAGTCGAACCGCTGGT10.23255813953488372No Hit
CCCAGGATCCTCGATCGCCTGCACGCCGACGA10.23255813953488372No Hit
CCCATCATACAGCCCCTCTG10.23255813953488372No Hit
CGGCACCACGAAGTGGGACCC10.23255813953488372No Hit
CATCCCCCCCACGTGCCCCAG10.23255813953488372No Hit
ATCGAGACCGGACTCCAAGCG10.23255813953488372No Hit
ATCGGCCGCCTGACTCC10.23255813953488372No Hit
CGGGTGAGGGAACCCGGCGCCACC10.23255813953488372No Hit
ATGGTGGCCTCTTCCCGCGGA10.23255813953488372No Hit
CGGGAGATCCGGGTAAACGCA10.23255813953488372No Hit
CTGAAGCGCTGTAAGGGCCCGC10.23255813953488372No Hit
AAACCGTCTCGGAGTGAAGAG10.23255813953488372No Hit
AGGCACCTTGGAAGAAAGAGGGGGATCCGGTAA10.23255813953488372No Hit
CACATCCGCCATCGCCGGGGG10.23255813953488372No Hit
ATCGTGTCCGAGTGCTTGGCG10.23255813953488372No Hit
CGAGTGGTTCCCGTTCGGGTC10.23255813953488372No Hit
TTCCCACGAGACACCTCCCCC10.23255813953488372No Hit
AGCCGGAGGCATCGAACGAC10.23255813953488372No Hit
CGGAACCGCGACGCAAGC10.23255813953488372No Hit
CGGAGATCGCGGCACTCGGCC10.23255813953488372No Hit
CGGGATCCTCGTCGACGGT10.23255813953488372No Hit
TATCACCCGGATGGGGGCAG10.23255813953488372No Hit
CCCAGGATCCTCGATCGCCTGCACGCCGACGAA10.23255813953488372No Hit
TCGAGACCGGACTCCAAGCGAGGGAAG10.23255813953488372No Hit
CGAAGTTTCCTTGTCTCCGCTCGTCGTGC10.23255813953488372No Hit
CAGCCTCGGAGCCCTGCTCTG10.23255813953488372No Hit
CGTTGGCGGCCCCTTTCCCAC10.23255813953488372No Hit
GAGTGAAGAGCGGCCGCGCTG10.23255813953488372No Hit
CCATCCCCCCCACGTGCCCCA10.23255813953488372No Hit
CGCGGTGGCCAGGTTGTAGAG10.23255813953488372No Hit
GAGGGGGATCCGGTACTGTCG10.23255813953488372No Hit
TTCCGGATTGCGTCGGCCGCG10.23255813953488372No Hit
TGAGGGGCCCTCCGTATACCA10.23255813953488372No Hit
TTGTTCTTGCCGCGCCTCTGA10.23255813953488372No Hit
CCCTGTCTCCACGGGTCGGACC10.23255813953488372No Hit
CCCTCACGGGGATGTC10.23255813953488372No Hit
TTGGCGGCCCCTTTCCCACGAG10.23255813953488372No Hit
CGGATTGCGTCGGCCGCGAC10.23255813953488372No Hit
AAAGAGGGGGATCCGGTACTGTCGA10.23255813953488372No Hit
ACTGTCGGAACCGCGACGCACC10.23255813953488372No Hit
CGGGGCATAGCGAGCACGGG10.23255813953488372No Hit
ACCTCGCAGCGGATCC10.23255813953488372No Hit
GATTGCGTCGGCCGCGACACCCCAGT10.23255813953488372No Hit
TCCCATACCCGCCGGAGATCGC10.23255813953488372No Hit
GGAGATCGCGGCACTCGGCC10.23255813953488372No Hit
CGGCGCTTCCCCGGAGACTCCTGACGACATGGT10.23255813953488372No Hit
GCCGCGCTGCGCTCCAAGAAC10.23255813953488372No Hit
CTGCTCTGGCGGAGGCACCGT10.23255813953488372No Hit
TGTTCGGAGAGCCACATCGGC10.23255813953488372No Hit
CCAGTGGGCAGGCGGAGCCGT10.23255813953488372No Hit
GACTCCGATCTACGGGCGGCC10.23255813953488372No Hit
ACCGAACCATCCGGATCCCGC10.23255813953488372No Hit
CTTCCCGCGGAGGCGAGGGCCCGCCCGAACCC10.23255813953488372No Hit
CAAATGGTGGAGGGGG10.23255813953488372No Hit
GACACCTCCCCCAGCTCCTTG10.23255813953488372No Hit
CATGAGGTCTGTGGCGGCAGT10.23255813953488372No Hit
ATACCCACCGCCATCGCTCGTTCGTCCTGT10.23255813953488372No Hit
CACATCGGCCACCACCTGGAG10.23255813953488372No Hit
ATTTGTCCGCGAAGGGGGCCT10.23255813953488372No Hit
GAGAGATCTCCGGAGGACGG10.23255813953488372No Hit
GGCATGAGGTCTGTGGCGGCAC10.23255813953488372No Hit
CGAGGAACAGACGGCGCGCAGT10.23255813953488372No Hit
CCTACCCTCCCGAAACTCCCC10.23255813953488372No Hit
TGTAAGGGCCCGCGGATCACG10.23255813953488372No Hit
CGGGGCATAGCGAGCACGGGT10.23255813953488372No Hit
AGCGATCTGCTCGGGCGAGA10.23255813953488372No Hit
GGTTCGCTCTGGGTCGTCGCC10.23255813953488372No Hit
AGGGGCGCCGCCCAGTCGCTC10.23255813953488372No Hit
AGGCGTCAGGCGATCGATAGG10.23255813953488372No Hit
CAGAGACCGGAGAAGAGGGGA10.23255813953488372No Hit
ATCCGCATCGGGATCCTCGTC10.23255813953488372No Hit
CCAGGATCCTCGATCGCCTGCACGCCGACGA10.23255813953488372No Hit
TCCGGATCCCGCCGGACGCGG10.23255813953488372No Hit
ACATCCGCCATCGCCGGGGGC10.23255813953488372No Hit
AGGCATGAGGTCTGTGGCGGCA10.23255813953488372No Hit
TCCCTCGGAGACCCCCCACTTC10.23255813953488372No Hit
CGACGAGAAGGATCCAAGGAC10.23255813953488372No Hit
CGATAGCCTCCTCCATCGGGA10.23255813953488372No Hit
CGCTCGTTCGTCCTGTCTCGA10.23255813953488372No Hit
AGGAGTCCCATACCCGCCGGAGATCGCGGCACTC10.23255813953488372No Hit
ATCGCGGCACTCGGCCTGAGTGG10.23255813953488372No Hit
AACTCTTTCGGTCCCGCTGCT10.23255813953488372No Hit
TGTCTCCGCTCGTCGTGCCCCTC10.23255813953488372No Hit
TCGGGATCCTCGTCGACGGTG10.23255813953488372No Hit
GCGGCGGACGACGAGA10.23255813953488372No Hit
AATGGCCTTCCGGATTGCGTC10.23255813953488372No Hit
CGACAGATCGAGACCGGACTC10.23255813953488372No Hit
AAGGGACGGATTCTCCGGGGGTCTCGACC10.23255813953488372No Hit
CGAGGAGTACCTGGCCCACCA10.23255813953488372No Hit
TTCCGGATTGCGTCGGCCGCGC10.23255813953488372No Hit
TCCAAGAACGCCGTGCGAGAC10.23255813953488372No Hit
GACGCCCGAGTGGTTCCCGTT10.23255813953488372No Hit
GCGAGGGTCGGGGCGTTGGCG10.23255813953488372No Hit
CCCGGAGACTCCTGACGAC10.23255813953488372No Hit
CGCATCGGGATCCTCGTCGAC10.23255813953488372No Hit
CTGCTCTGGCGGAGGCACCGTT10.23255813953488372No Hit
ACGGCGCGACAGAGACCGGACC10.23255813953488372No Hit
GTACATGACCCCACCGGCGC10.23255813953488372No Hit
GCGGAGTACATGACCCCACCGGGA10.23255813953488372No Hit
AAGGTCGACGCGGGGCTCCGC10.23255813953488372No Hit
CAGATCGAGACCGGACTCCAAGC10.23255813953488372No Hit
CCCAGTCGCTCGTCATCGAAG10.23255813953488372No Hit
CGGTCCCGCTGCTATCCACCGAACCATCCGGATCC10.23255813953488372No Hit
TTCCCCGGAGACTCCTGACGAC10.23255813953488372No Hit
CGCGGATCACGAGCGAGGGTC10.23255813953488372No Hit
AGAGGGGGATCCGGTACTGTC10.23255813953488372No Hit
TACCGCGGCCGCGCGCACCGGAAG10.23255813953488372No Hit
CCTCCTCCTCCTCCT10.23255813953488372No Hit
AGCGATCTGCTCGGGCGAGAA10.23255813953488372No Hit
ATCCTCGATCGCCTGCACGCC10.23255813953488372No Hit
GTCCGTAGGCATGAGGTCTGT10.23255813953488372No Hit
GTATCCCGCCCTGATGTCATC10.23255813953488372No Hit
ACGACTCTCGCCTTATTGCCC10.23255813953488372No Hit
CGATAGGACTTCGCGCGGTTC10.23255813953488372No Hit
CTTGTTCTTGCCGCGCCTCTG10.23255813953488372No Hit
CCACGGGTCGGAGTCCACCTT10.23255813953488372No Hit
ATCGGGATCCTCGTCGACGGT10.23255813953488372No Hit
AGGACGTTCGAGCTCCTCGGA10.23255813953488372No Hit
CGAGAAGGATCCAAGGACGGAT10.23255813953488372No Hit
CTCGGCCTGAGTGGACGCGCC10.23255813953488372No Hit
CTTCGAGAAGCTCCGAGCGCGC10.23255813953488372No Hit
CCGAAAGGTCGACGCGGGGCTCTCT10.23255813953488372No Hit
GGGCGAGAAGGCTGCGGCCA10.23255813953488372No Hit
GTCGACGCGGGGCTCCGCCTC10.23255813953488372No Hit
AGGAGTCCCATACCCGCCGGCC10.23255813953488372No Hit
CACGAGTGGTCAGAGGGCC10.23255813953488372No Hit
CGAGAGATCTCCGGAGGACGGT10.23255813953488372No Hit
GCCTTCCGGATTGCGTCGGCCGCGACACCCC10.23255813953488372No Hit
TCCAAGAACGCCGTGCGAGACC10.23255813953488372No Hit
ATGCCCCAGTGGAAGAGGCGTCGCTCCT10.23255813953488372No Hit
CGAGCGACTGGTCTCGGAAGCCCGAGAGA10.23255813953488372No Hit
CACGGGTCTCGGAAGGGCGGG10.23255813953488372No Hit
GGGGCCAGGCCCACCGTGCGG10.23255813953488372No Hit
GGCGAGCGCTCTTCGTATGACGG10.23255813953488372No Hit
GATCGAGACCGGACTCCAAGC10.23255813953488372No Hit
ATCGGGATCCTCGTCGACGG10.23255813953488372No Hit
TTTACGGGTGGGCCTCTTCCT10.23255813953488372No Hit
TACATGACCCCACCGGCGCTT10.23255813953488372No Hit
TCCTACGACCTCCACGTGCGT10.23255813953488372No Hit
AGGTTCCATACCCTCCCGAAACTCCCCATCA10.23255813953488372No Hit
ATGGTCGATTACAGCGGAGAAATCT10.23255813953488372No Hit
CGCGAAGGGGGCCTGGAGG10.23255813953488372No Hit
AGGTTCCCTACCCTCCCGAAA10.23255813953488372No Hit
TAGCAGCGGGAAGGGTGAGA10.23255813953488372No Hit
ACGGGGATGTCGAGGAGCG10.23255813953488372No Hit
GCACGCCGACGAATCCCTCACC10.23255813953488372No Hit
CAGCAAGAGACAGACCCGAGC10.23255813953488372No Hit
CCGAACCATCCGGATCCCGCC10.23255813953488372No Hit
ACGGCGCGCAGGGTTTCCCTC10.23255813953488372No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)