FastQCFastQC Report
Mon 30 Nov 2020
S33.raw.14987.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.raw.14987.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18330
Sequences flagged as poor quality0
Sequence length11-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAGGAGGAGAGGTCTCCCCTCTTCTCCGGTCTCTGTCGCGCC2711.4784506273867977No Hit
ATCTGTCGAGACAGGACGAACGAGCGATGGCGGTGGGTATCCT1610.878341516639389No Hit
ATCTGTCGAGACAGGACGAACGAGCGATGGCGGTGGGTATCCTTCGCTTGCGCGAATGGATCTCCA1340.7310420076377523No Hit
ATTATTACTGGGCCCGCTTCTGGACCGAGACCCGAACGGGAACCACTCGG930.5073649754500819No Hit
CTATCGCCTCCCGGGTGGCCAAGGATCTCCTCTG860.4691762138570649No Hit
CGTGGTGCCGGAACAGCTGCTGGACC850.4637206764866339No Hit
ATGTGGCTCTCCGAACAGTCGCCGCGGAGGCACGCGCCGAGGGCGGCCATCGGCTTCCGGTGCGCGCGGCCGCGG700.3818876159301691No Hit
CTGCCCCCGGCGATGGCGGATGTGCTGTACTCTGCTGAGGAGGAGAGGTC700.3818876159301691No Hit
CGATGTGGCTCTCCGAACAGTCGCCGCGGAGGCACGCGCCGAGGGCGGCC590.32187670485542824No Hit
ATCTGTCGAGACAGGACGAACGAGCGATGGCGGTGGGT550.3000545553737043No Hit
GGGGTCATGGCTGAACCACGACTTGGTAGAACGGGC480.2618657937806874No Hit
TGAGGACCCCGCTGCTCTTCGAGCGACTGGTCGGGCGGACAGTGGCCCCT470.2564102564102564No Hit
GCGCTCGCCAAGCACTCGGACACGATTGGGTCCCACTTCGTGGTGCCGGAACAGCTGCTGGACC460.2509547190398254No Hit
GGGGTCATGGCTGAACCACGACTTGGTAGAACGGGCAGTT450.24549918166939444No Hit
CGGACACGATTGGGTCCCACTTCGTGGTGCCGGAACAGCTGCTGGACC450.24549918166939444No Hit
TGCGTTTACCCGGATCTCCCGGGCCCAGACGGCCCTCTGACC420.22913256955810146No Hit
GAAGTCCTATCGATCGCCTGACGCCTTCGTCCTCGCAGAGAAGA420.22913256955810146No Hit
ATTATTACTGGGCCCGCTTCTGGACCGAGACCCGAACGGGAACCACTCGGGCGTCCGTCCTTGG410.2236770321876705No Hit
TTCGAAGCCGGAATGCTGAAGTGGGGGGTCTCCGAGG410.2236770321876705No Hit
GAAACCCTGCGCGCCGTCTGTTCCTCGCACAGCTCGCTCGCCTCGGTCGTTCGATGCCTCCGGCTGACGACGCCG350.19094380796508456No Hit
ATGAGACGTTGCGTCTGGTCAAGTCCTACTCCGAATACTGCCGCGCTCGCGCTCTGGGCGCGGACCAGGCGGC350.19094380796508456No Hit
ATCCGTCCCTTCGATGACGAGCGACTGGGCGGCGCCCCTGGA330.18003273322422259No Hit
AAACCCTGCGCGCCGTCTGTTCCTCGCACA330.18003273322422259No Hit
CTGCCCACTGGGGTGTCGCGGCC330.18003273322422259No Hit
ACTCGGACACGATTGGGTCCCACTTCGTGGTGCCGGAACAGCTGCTGGACC320.1745771958537916No Hit
ATCTTGAACCTCTACAACCTGGCCACCGCGGACCTCGCCTGTGTCGACGG320.1745771958537916No Hit
TATTATTACTGGGCCCGCTTCTGGACCGAGACCCGAACGGGAACCACTCGGGCGTCCGTCCTTGGATCCTTCTCG310.1691216584833606No Hit
AACCCTGCGCGCCGTCTGTTCCTCGCACA310.1691216584833606No Hit
CTCGGACACGATTGGGTCCCACTTCGTGGTGCCGGAACAGCTGCTGGACC310.1691216584833606No Hit
AAGCACTCGGACACGATTGGGTCCCACTTCGTGGTGCCGGAACAGCTGCTGGACC300.16366612111292964No Hit
GAAGCCGGAATGCTGAAGTGGGGGGTCTCCGAGG300.16366612111292964No Hit
ATCTGTCTTGGGTCATCTTGAACCTCTACAACCTGGCCACCGCGGACCTCGC300.16366612111292964No Hit
CAGGCCGAGTGCCGCGATCTCCGGCGGGTATGGGACTC300.16366612111292964No Hit
ATGAGACGTTGCGTCTGGTCAAGTCCTACTCCGAATACTGCCGCGCTCGCGCTCTGGGCGCGGACCAGGCGGCG280.15275504637206766No Hit
CGCCGTCATACGAAGAGCGCTCGCCAAGCACTCGGACACGATTGGGTCCCACTTCGTGGTGCCGGAACAGCTGC280.15275504637206766No Hit
ATGAGACGTTGCGTCTGGTCAAGTCCTACTCCGAATACTGCCGCGCTCGCGCTCTGGGCGCGGACCAGGCGGCGC280.15275504637206766No Hit
GCACTCGGACACGATTGGGTCCCACTTCGTGGTGCCGGAACAGCTGCTGGACC280.15275504637206766No Hit
TTTCGGGAGGGTAGGGAACCTGGTATACGGAGGGCCCCTCACGGGGTCGTCCGTCCTCCGGAGATCTCTCGGGCT270.14729950900163666No Hit
CGAGCAGATCGCTCGGTACGAGTGGCTGATCACCGCGACCGGCGGCGAGG270.14729950900163666No Hit
CTATCCTTGGCCAAGTCGACTGCGTTTACCCGGATCTCCCGGGCCCAGACGGCCCTCTGACC270.14729950900163666No Hit
GGGGTCATGGCTGAACCACGACTTGGTAGAACGGGCA270.14729950900163666No Hit
CTACAACCTGGCCACCGCGGACCTCGCCTGTGTCGACG270.14729950900163666No Hit
CTTCCGTCTGGTCCTCCGAAGCCT270.14729950900163666No Hit
CTATCCTTGGCCAAGTCGACTGCGTTTACCCGGATCTCCCGGGCCCAGAC260.14184397163120568No Hit
CGATGGCGGATGTGCTGTACTCTGCTGAGGAGGAGAGGTCTCCCCTCTTCTCCGGTCTCTGTCGCGCC260.14184397163120568No Hit
CTTGAACCTCTACAACCTGGCCACCGCGGACCTCGCCTGTGTCGACGG260.14184397163120568No Hit
CAGGACGAACGAGCGATGGCGGTGGGTATCCTTCGCTTGCGCGAATGGATCTCCA260.14184397163120568No Hit
ATCTTGAACCTCTACAACCTGGCCACCGCGGACCTCGCCTGTGTCGACGGG260.14184397163120568No Hit
ATCTGTCGAGACAGGACGAACGAGCGATGGCGGTGGGTATCCTTCGCTTGCGCGAATGGATCTCCACCGCTTGGG250.13638843426077468No Hit
TTCGGGAGGGTAGGGAACCTGGTATACGGAGGGCCCCTCACGGGGTCGTCCGTCCTCCGGAGATCTCTCGGGCTT250.13638843426077468No Hit
ATATCGAGGAATTCGACCTCCAGGTGGTGGCCGATGTGGCTCTCCGAACA250.13638843426077468No Hit
CTTATTACGGCCCTTTGGCGTGGTCTCTGTGACGGCCT240.1309328968903437No Hit
GACCCCGCTGCTCTTCGAGCGACTGGTCGGGCGGACAGTGGCCCCTCTCGGCACGCACGTGGAGGTCGTAGG230.1254773595199127No Hit
AACCCTGCGCGCCGTCTGTTCCTCGCACAGCTCGCTCGCCTCGGTCGTTC230.1254773595199127No Hit
GAGGACCCCGCTGCTCTTCGAGCGACTGGTCGGGCGGACAGTGGCCCCTC230.1254773595199127No Hit
CAAGGTGGACTCCGACCCGTGGAGACAGGGCGACGACCCAGAGCGAACCC230.1254773595199127No Hit
GAGGACCCCGCTGCTCTTCGAGCGACTGGTCGGGCGGACAGTGGCCCCTCTCGGCACGCACGTGGAGGTCGTAGG230.1254773595199127No Hit
AATGAGACGTTGCGTCTGGTCAAGTCCTACTCCGAATACTGCCGCGCTCG230.1254773595199127No Hit
TTCGAAGCCGGAATGCTGAAGTGGGGGGTCTCCGAGGG230.1254773595199127No Hit
CATCTTGAACCTCTACAACCTGGCCACCGCGGACCTCGCCTGTGTCGACGG220.12002182214948172No Hit
CTGCGCGCCGTCTGTTCCTCGCACAGCTCGCTCGCCTCGGTC220.12002182214948172No Hit
AGACGTTGCGTCTGGTCAAGTCCTACTCCGAATACTGCCGCGCTCGCGCTCTGGGCGCGGACCAGGCGGC220.12002182214948172No Hit
CTTGGGTCATCTTGAACCTCTACAACCTGGCCACCGCGGACCTCGCCTGTGTCGACGG220.12002182214948172No Hit
ATCTTGAACCTCTACAACCTGGCCACCGCGGACCTCGCCTGTGTCGACGGGG220.12002182214948172No Hit
AATCTGTCTTGGGTCATCTTGAACCTCTACAACCTGGCCACCGCGGACCTCGCCTGTGTCGACGG220.12002182214948172No Hit
ATCTTGAACCTCTACAACCTGGCCACCGCGGACCTCGC210.11456628477905073No Hit
CGAGGAATTCGACCTCCAGGTGGTGGCCGATGTGGCTCTCCGAACAGTCG210.11456628477905073No Hit
CTTATTACGGCCCTTTGGCGTGGTCTCTGTGACGGCCTCAAGGTCGGCGTGCGTGACACCTTCCGGTTGGCCGGC210.11456628477905073No Hit
CTTATTACGGCCCTTTGGCGTGGTCTCTGTGACGGCCTC210.11456628477905073No Hit
GAAGTCCTATCGATCGCCTGACGCCTTCGTCCTCGCAGA210.11456628477905073No Hit
CGTAAAGCGCTGGGATCTTAAGTTTGAGATTTCTC210.11456628477905073No Hit
TCGGACACGATTGGGTCCCACTTCGTGGTGCCGGAACAGCTGCTGGACC210.11456628477905073No Hit
CGCACTCAAGGTGGACTCCGACCCGTGGAGACAGGG200.10911074740861974No Hit
CTTCGTCAAAGCTCCCCTGCCCCCATCCGG200.10911074740861974No Hit
ATCTGTCTTGGGTCATCTTGAACCTCTACAACCTGGCCACCGCGGACCTCGCCTGTGTCGACGG200.10911074740861974No Hit
CCCTTCGATGACGAGCGACTGGGCGGCGCCCCTGGAA200.10911074740861974No Hit
ACCATGTCGTCAGGAGTCTCCGGGGAAGCGCCGGTGGGGTCATGTACTCCGCCGACCTGACTGCCGCCACAGACC200.10911074740861974No Hit
CTTGAACCTCTACAACCTGGCCACCGCGGACCTCGCCTGTGTCGACGGG190.10365521003818876No Hit
TGAGGAGGAGAGGTCTCCCCTCTTCTCCGGTCTCTGTCGCGCC190.10365521003818876No Hit
CTTGGGTCATCTTGAACCTCTACAACCTGGCCACCGCGGACCTCGC190.10365521003818876No Hit
CTGAGGAGGAGAGGTCTCCCCTCTTCTCCGGTCTCTGTCGCGCCGTACTGA190.10365521003818876No Hit
CTACAACCTGGCCACCGCGGACCTCGC190.10365521003818876No Hit
CTATCCTTGGCCAAGTCGACTGCGTTTACCCGGATCTC190.10365521003818876No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)