FastQCFastQC Report
Mon 30 Nov 2020
S34.15-35nt.14987.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.15-35nt.14987.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9584
Sequences flagged as poor quality0
Sequence length15-35
%GC67

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGGACCGAGACCCGAACGG1811.8885642737896495No Hit
TGGCCACCGCGGACCTCGCCT1141.189482470784641No Hit
CCGTCTGGTCCTCCGAAGCCT971.0121035058430716No Hit
CGTGGTGCCGGAACAGCTGCTGGACC810.8451585976627712No Hit
TCCTATCGATCGCCTGACGCC750.7825542570951586No Hit
TCGGGCCGCAGAGACGTCGCC630.6573455759599331No Hit
CCTATCGATCGCCTGACGCCT600.6260434056761269No Hit
GGCGATCGAGGATCCTGGGGC590.6156093489148581No Hit
CCTGGCCACCGCGGAC520.5425709515859767No Hit
CGGGGGTCGTCCGTC490.5112687813021703No Hit
CGAGGCTGTCCCGGATCTCCG480.5008347245409015No Hit
CTTCCGTCTGGTCCTCCGAAGCCT430.44866444073455763No Hit
TACAACCTGGCCACCGCGGAC360.3756260434056761No Hit
GGCACGCACGTGGAGGTCGTAGGAGA350.3651919866444074No Hit
ACTGGGGCATCGGTCAGGCGA350.3651919866444074No Hit
CGTGCGTGACACCTTCCGGTTGGCCGGCCTGG350.3651919866444074No Hit
ACCTGGCCACCGCGGAC290.30258764607679467No Hit
GCCCACTGGGGTGTCGCGGCC290.30258764607679467No Hit
CGGAACAGCTGCTGGACC290.30258764607679467No Hit
CTGGTATACGGAGGGCCCCTC290.30258764607679467No Hit
TCGTGGTGCCGGAACAGCTGCTGGACC280.2921535893155259No Hit
ATCGAGGATCCTGGGGCACG280.2921535893155259No Hit
GTGGTGCCGGAACAGCTGCTGGACC280.2921535893155259No Hit
CTCCGGCGGGTATGGGACTCCTTCC270.2817195325542571No Hit
AGGTCGGCGTGCGTGACACCTTCCGGTTGGCC270.2817195325542571No Hit
CTGGACCGAGACCCGAACGGG260.27128547579298834No Hit
CTGCCCACTGGGGTGTCGCGGCC260.27128547579298834No Hit
CCTGGCCACCGCGGACCTCGCCT260.27128547579298834No Hit
AGTCGCTCGGGTCTGTCTCTTGCTGAG250.26085141903171954No Hit
CCGGTCCGTCCGACGAAGGGC240.25041736227045075No Hit
TTCTCCGGTCTCTGTCGCGCC240.25041736227045075No Hit
AAGGTGGACTCCGACCCGTGG230.23998330550918195No Hit
TTCGATGACGAGCGACTGGGCGGCGCCC220.2295492487479132No Hit
CCCACTTCGTGGTGCCGGAACAGCTGCTGGACCA220.2295492487479132No Hit
CTGGCCACCGCGGACCTCGCCT220.2295492487479132No Hit
AACGGAAGGCGCTCTGGGATT220.2295492487479132No Hit
TGCCGGAACAGCTGCTGGACC220.2295492487479132No Hit
CGTCTGGTCCTCCGAAGCCT210.2191151919866444No Hit
GCCGGAACAGCTGCTGGACC200.20868113522537562No Hit
CGTGGTGCCGGAACAGCTGCTGGACCA200.20868113522537562No Hit
TGCGCGCCGTCTGTTCCTCGC200.20868113522537562No Hit
CTCGTCGTCCGCCGCTGTGGC200.20868113522537562No Hit
ACGAGCGACTGGGCGGCGCCC190.19824707846410683No Hit
AGTAATCCCCCGCGTCCGGCG190.19824707846410683No Hit
GGGATTCGTCGGCGTGCAGGC190.19824707846410683No Hit
CGACCGCTGTCCCGATGGAGG190.19824707846410683No Hit
AAGGTCGGCGTGCGTGACACCTTCCGGTTGGCC180.18781302170283806No Hit
ACCGCGGACCTCGCCTGTGTC180.18781302170283806No Hit
AACCTGGCCACCGCGGAC180.18781302170283806No Hit
CTACAACCTGGCCACCGCGGAC180.18781302170283806No Hit
ACAACGGAAGGCGCTCTGGGA180.18781302170283806No Hit
AGGTGGACTCCGACCCGTGGA180.18781302170283806No Hit
CAGGACGAACGAGCGATGGCGGTGGGTATCCT180.18781302170283806No Hit
GGCACGCACGTGGAGGTCGTAGGA180.18781302170283806No Hit
ATCCTTCTCGTCGTCCGCCGCTGTGGC180.18781302170283806No Hit
GAGGAGGAGATCGCGGCGGAT170.1773789649415693No Hit
ACAGCTCGCTCGCCTCGGTC170.1773789649415693No Hit
CCGGCGGGTATGGGACTCCTTCC160.1669449081803005No Hit
CAACGGAAGGCGCTCTGGGAT160.1669449081803005No Hit
AGGAGGAGGAGATCGCGGCGG160.1669449081803005No Hit
CTCGCTCGCCTCGGTCGTTCG160.1669449081803005No Hit
GAGTAATCCCCCGCGTCCGGC160.1669449081803005No Hit
AAACCCTCCCCCTGGCCCTGT160.1669449081803005No Hit
CGGGTTACCACGTTCGGTTCC160.1669449081803005No Hit
CGCACTCAAGGTGGACTCCGACCC160.1669449081803005No Hit
TCTCGTCGTCCGCCGCTGTGG160.1669449081803005No Hit
AAGAGGCCACCATCGAAGGGC160.1669449081803005No Hit
GAAGGCGCTCTGGGATTTGGC160.1669449081803005No Hit
CGGCGTGCGTGACACCTTCCGGTTGGCCGGCCTGG150.15651085141903173No Hit
AGGCGACCGCTGTCCCGATGGAGGAG150.15651085141903173No Hit
GACGAGCGACTGGGCGGCGCCCCT150.15651085141903173No Hit
TCCGGCGGGTATGGGACTCCTTCC150.15651085141903173No Hit
CAGGACGAACGAGCGATGGCG150.15651085141903173No Hit
CTTCCGTCTGGTCCTCCGAAGCCTCTTCCCT150.15651085141903173No Hit
CTATCGCCTCCCGGGTGGCCAAGGATCTCCTCTG150.15651085141903173No Hit
TTTGGCGCGTCCTGAGCTGGA140.14607679465776294No Hit
CTGGACCGAGACCCGAACGG140.14607679465776294No Hit
CACCGTCGACGAGGATCCC140.14607679465776294No Hit
TGAGGACCCCGCTGCTCTTCGAGCGACT140.14607679465776294No Hit
GGCGTGCAGGCGATCGAGGAT140.14607679465776294No Hit
CCACTTCGTGGTGCCGGAACAGCTGCTGGACC140.14607679465776294No Hit
GAGGACCCCGCTGCTCTTCGAGCGACT140.14607679465776294No Hit
TGACCTGGCCGCAGCCTTCTCGCCCGA140.14607679465776294No Hit
CGGAATGCTGAAGTGGGGGGT140.14607679465776294No Hit
CACAGCTCGCTCGCCTCGGTC130.13564273789649417No Hit
TGATAAACCCTCCCCCTGGCC130.13564273789649417No Hit
ATCCTTCTCGTCGTCCGCCGCTGTGG130.13564273789649417No Hit
CAAGGTCGGCGTGCGTGACACCTTCCGGTTGGCC130.13564273789649417No Hit
CTGAGGACCCCGCTGCTCTTCGAGCGACT130.13564273789649417No Hit
ATAAACCCTCCCCCTGGCCCT130.13564273789649417No Hit
ACCGCTGTCCCGATGGAGGAG130.13564273789649417No Hit
CCGAACAGTCGCCGCGGAGGC120.12520868113522537No Hit
CGCCTCCCGGGTGGCCAAGGATCTCCTCTG120.12520868113522537No Hit
GCGCCGGTGGGGTCATGTACTC120.12520868113522537No Hit
GATAAACCCTCCCCCTGGCC120.12520868113522537No Hit
AGGACGAACGAGCGATGGCGG120.12520868113522537No Hit
TCCTTCTCGTCGTCCGCCGCT120.12520868113522537No Hit
CGTCAAAGCTCCCCTGCCCCC110.1147746243739566No Hit
TGGACCGAGACCCGAACGGGA110.1147746243739566No Hit
CCCACTTCGTGGTGCCGGAACAGCTGCTGGACC110.1147746243739566No Hit
CTGGCCACCGCGGACCTCGCC110.1147746243739566No Hit
GAAGTCCTATCGATCGCCTGACGCCTTCGTCCT110.1147746243739566No Hit
GATAAACCCTCCCCCTGGCCC110.1147746243739566No Hit
TGGCGTGGTCTCTGTGACGGC110.1147746243739566No Hit
TCGAACGTCTTCGGAAGGGGG110.1147746243739566No Hit
CCGGAACAGCTGCTGGACCAG110.1147746243739566No Hit
GGCCACCGCGGACCTCGCCT110.1147746243739566No Hit
CCCTGAGGGATTCGTCGGCGTGCAGGC110.1147746243739566No Hit
AAACCCTGCGCGCCGTCTGTT110.1147746243739566No Hit
ATACGAAGAGCGCTCGCCAAG100.10434056761268781No Hit
TCCCGCACGGTGGGCCTGGCC100.10434056761268781No Hit
GCGCTTCAGCTGAGGACCCCG100.10434056761268781No Hit
TCCGAATACTGCCGCGCTCGC100.10434056761268781No Hit
GAACGAGCGATGGCGGTGGGT100.10434056761268781No Hit
CGCCTGCAGGGCGGACAACGGAAGGCGCTCTGGG100.10434056761268781No Hit
CTTCGATGACGAGCGACTGGGCGGCGCCC100.10434056761268781No Hit
ATCCTTCTCGTCGTCCGCCGC100.10434056761268781No Hit
CTCCGGCGGGTATGGGACTCCTTC100.10434056761268781No Hit
CAGGCGACCGCTGTCCCGATGGAGGAGG100.10434056761268781No Hit
CGAACGAGCGATGGCGGTGGG100.10434056761268781No Hit
AGCTCCCCTGCCCCCATCCGG100.10434056761268781No Hit
GAGGGATTCGTCGGCGTGCAG100.10434056761268781No Hit
TCCGTCTGGTCCTCCGAAGCCT100.10434056761268781No Hit
AGGCGATCGAGGATCCTGGGG100.10434056761268781No Hit
CCCCCTGAGGGATTCGTCGGCGTGCAGGC100.10434056761268781No Hit
GGAGGAGGAGGAGATCGCGGCGGAT100.10434056761268781No Hit
GATCGAGGATCCTGGGGCACG100.10434056761268781No Hit
GGTCGGCGTGCGTGACACCTTCCGGTTGGCCGGC100.10434056761268781No Hit
TCCCACTTCGTGGTGCCGGAACAGCTGCTGGACC100.10434056761268781No Hit
CCGGAATGCTGAAGTGGGGGG100.10434056761268781No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)