FastQCFastQC Report
Mon 30 Nov 2020
S34.15-35nt.14987.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.15-35nt.14987.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4292
Sequences flagged as poor quality0
Sequence length15-35
%GC67

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGAGATCTCCGGAGGACGG2686.244175209692451No Hit
TCCCCGGAGACTCCTGACGAC1543.5880708294501398No Hit
GGAGATCGCGGCACTCGGCCT1293.0055917986952467No Hit
CGAGAAGGATCCAAGGACGGA1202.7958993476234855No Hit
TTCCCCGGAGACTCCTGACGA1072.4930102516309414No Hit
ACGAGAAGGATCCAAGGACGG641.4911463187325256No Hit
TCCTCGATCGCCTGCACGCCG631.4678471575023297No Hit
GAGTACATGACCCCACCGGCG501.1649580615097856No Hit
TCGGGCGAGAAGGCTGCGGCC410.9552656104380242No Hit
TCCGGCGACGTCTCTGCGGCC330.7688723205964585No Hit
CATCGGGATCCTCGTCGACGG310.7222739981360671No Hit
TAAGGGCCCGCGGATCACGAG300.6989748369058714No Hit
TCATCCAGCTCAGGACGCGCC270.6290773532152842No Hit
CGACGAGAAGGATCCAAGGACC240.5591798695246971No Hit
CGCATCGGGATCCTCGTCGAC240.5591798695246971No Hit
CCGGAGATCGCGGCACTCGGC220.5125815470643057No Hit
CGACGTCTCTGCGGCCCGAAA210.48928238583411No Hit
CGGAGATCGCGGCACTCGGCC210.48928238583411No Hit
CCCAGGATCCTCGATCGCCT210.48928238583411No Hit
TGTAAGGGCCCGCGGATCACG210.48928238583411No Hit
CTCGGAGATCCGGGACAGCCT200.4659832246039142No Hit
TCCGCTCGTCGTGCCCCTCGG200.4659832246039142No Hit
AGCACGAGTGGTCAGAGGGCC190.4426840633737186No Hit
CCGAGAGATCTCCGGAGGACG180.4193849021435228No Hit
GCACATCCGCCATCGCCGGGG170.39608574091332716No Hit
CGGGGTCCTCAGCTGAAGCGC170.39608574091332716No Hit
TGCTTGGCGAGCGCTCTTCGT170.39608574091332716No Hit
TCCCCGGAGACTCCTGACGACA170.39608574091332716No Hit
CCGGATTGCGTCGGCCGCGAC170.39608574091332716No Hit
CGGAGATCCGGGACAGCCTCG160.3727865796831314No Hit
CGGAGACTCCTGACGACATGG160.3727865796831314No Hit
AGGACTTCGCGCGGTTCGCCT150.3494874184529357No Hit
ATCGAGACCGGACTCCAAGCG140.32618825722274No Hit
AGGATCCTCGATCGCCTGCAC130.30288909599254427No Hit
TGTCTCCGCTCGTCGTGCCCC130.30288909599254427No Hit
CACGTGCCCCAGGATCCTCGA130.30288909599254427No Hit
ACATCGGCCACCACCTGGAGG130.30288909599254427No Hit
TTCCCCGGAGACTCCTGACGAC130.30288909599254427No Hit
CAAGGACGGACGCCCGAGTGG130.30288909599254427No Hit
AAGGGCCCGCGGATCACGAGC130.30288909599254427No Hit
GACGCCCGAGTGGTTCCCGTT130.30288909599254427No Hit
ATCGGGATCCTCGTCGACGGT120.27958993476234856No Hit
TACAGCCCCTCTGAGCACGAG120.27958993476234856No Hit
GATCGAGACCGGACTCCAAGC120.27958993476234856No Hit
TCCCCCCCACGTGCCCCAGGA120.27958993476234856No Hit
TCGGGGCATAGCGAGCACGGG110.25629077353215285No Hit
CGACGAGAAGGATCCAAGGAC110.25629077353215285No Hit
CTCGGCCTGAGTGGACGCGCC110.25629077353215285No Hit
TCGGTCCCGCTGCTATCCACC100.2329916123019571No Hit
AGAAGCTCCGAGCGCGCACGG100.2329916123019571No Hit
TCCAAGAACGCCGTGCGAGAC100.2329916123019571No Hit
GCATCGGGATCCTCGTCGACG100.2329916123019571No Hit
GAAGCCAAGAGTGTATCCCGC100.2329916123019571No Hit
AAGCCAAGAGTGTATCCCGCC100.2329916123019571No Hit
AATGGCCTTCCGGATTGCGTC100.2329916123019571No Hit
ATCCTCGATCGCCTGCACGCC100.2329916123019571No Hit
ATCCGGGTAAACGCAGTCGAC100.2329916123019571No Hit
CCAGGATCCTCGATCGCCT100.2329916123019571No Hit
TCGGCCTGAGTGGACGCGCCC100.2329916123019571No Hit
TAAGGTCCGTAGGCATGAGGT90.2096924510717614No Hit
TCCGGCACCACGAAGTGGGAC90.2096924510717614No Hit
CTCGATCGCCTGCACGCCGAC90.2096924510717614No Hit
AAGGATCCAAGGACGGACGCC90.2096924510717614No Hit
CCCACGAGACACCTCCCCCAG90.2096924510717614No Hit
TTTCCCACGAGACACCTCCCC90.2096924510717614No Hit
CGATAGCCTCCTCCATCGGGA90.2096924510717614No Hit
AAGACGTTCGAGCTCCTCGGA90.2096924510717614No Hit
ATCCAAGGACGGACGCCCGAG90.2096924510717614No Hit
TACCAGGTTCCCTACCCTCCC90.2096924510717614No Hit
CGCACGGGTTCGCTCTGGGTC90.2096924510717614No Hit
TAGGACTTCGCGCGGTTCGCCT90.2096924510717614No Hit
GGTCCGTAGGCATGAGGTCTG90.2096924510717614No Hit
TCGGGATCCTCGTCGACGGTG90.2096924510717614No Hit
AACCTCGCAGCGGATCCGGCG90.2096924510717614No Hit
CCCACGTGCCCCAGGATCCTC80.1863932898415657No Hit
TTCGGAGGACCAGACGGAAGG80.1863932898415657No Hit
CAATATTTCCAGGGGCGCCGC80.1863932898415657No Hit
ATCCGGCGACGTCTCTGCGGC80.1863932898415657No Hit
CCCGGAGACTCCTGACGACAT80.1863932898415657No Hit
TCCCGAAACTCCCCATCACCC80.1863932898415657No Hit
TACCCACCGCCATCGCTCGTT80.1863932898415657No Hit
TCTTGCCGCGCCTCTGACGGG80.1863932898415657No Hit
CAGCAAGAGACAGACCCGAGC80.1863932898415657No Hit
CCACGAGACACCTCCCCCAGC80.1863932898415657No Hit
AGGAACAGACGGCGCGCAGGG80.1863932898415657No Hit
AGCGATCTGCTCGGGCGAGAA80.1863932898415657No Hit
TTGTCTCCGCTCGTCGTGCCC80.1863932898415657No Hit
CCCCAGGATCCTCGATCGCCT80.1863932898415657No Hit
AGTGGACGCGCCCAGCGGGCA80.1863932898415657No Hit
GGCAGCCCTTCGTCGGACGGA80.1863932898415657No Hit
ATTCCTCCCAAGCTTCACCCA80.1863932898415657No Hit
TCCGGATTGCGTCGGCCGCGA80.1863932898415657No Hit
CGAGTGGTTCCCGTTCGGGTC70.16309412861137No Hit
AGGAGTCCCATACCCGCCGGA70.16309412861137No Hit
CGAGAAGGATCCAAGGACGG70.16309412861137No Hit
TCACGCACGCCGACCTTGAGG70.16309412861137No Hit
TTGTCCGCGAAGGGGGCCTGG70.16309412861137No Hit
TCCACGGGTCGGAGTCCACCT70.16309412861137No Hit
GGATCCTCGATCGCCTGCAC70.16309412861137No Hit
GTCTCCGCTCGTCGTGCCCCT70.16309412861137No Hit
TCCAGCAGCTGTTCCGGCACC70.16309412861137No Hit
CACGGGTCTCGGAAGGGCGGG70.16309412861137No Hit
ACGAGACACCTCCCCCAGCTC70.16309412861137No Hit
GAGAGATCTCCGGAGGACGG70.16309412861137No Hit
CCAAGAACGCCGTGCGAGACA70.16309412861137No Hit
GGGGCCAGGCCCACCGTGCGG70.16309412861137No Hit
GAGACCGGACTCCAAGCGAGG60.13979496738117428No Hit
ACACCTCCCCCAGCTCCTTGC60.13979496738117428No Hit
CGAAAGGTCGACGCGGGGCTC60.13979496738117428No Hit
CACCGGAAGCCGATGGCCGCC60.13979496738117428No Hit
GCGACGTCTCTGCGGCCCGAAA60.13979496738117428No Hit
AAGAGACAGACCCGAGCGACT60.13979496738117428No Hit
ATACCCACCGCCATCGCTCGT60.13979496738117428No Hit
TCCGATCTACGGGCGGCCGAG60.13979496738117428No Hit
TCGGAGATCCGGGACAGCCTC60.13979496738117428No Hit
CGAGGAGTACCTGGCCCACCA60.13979496738117428No Hit
CGAGAGATCTCCGGAGGACG60.13979496738117428No Hit
GGTTCGCTCTGGGTCGTCGCC60.13979496738117428No Hit
GTACATGACCCCACCGGCGCT60.13979496738117428No Hit
CTCTTGTTCTTGCCGCGCCTC60.13979496738117428No Hit
ATGAGGTCTGTGGCGGCAGTC60.13979496738117428No Hit
GGTCCCGCTGCTATCCACCGA60.13979496738117428No Hit
CCCGGAGACTCCTGACGACA60.13979496738117428No Hit
CACGGGGGACCAGCAGCGCCG60.13979496738117428No Hit
CCACCGCCATCGCTCGTTCGT50.11649580615097856No Hit
CCTTGAGGCCGTCACAGAGAC50.11649580615097856No Hit
TTCGAGAAGCTCCGAGCGCGC50.11649580615097856No Hit
AATATTTCCAGGGGCGCCGCC50.11649580615097856No Hit
CGCGAGATCTCCGGAGGACGG50.11649580615097856No Hit
AGGGGTCAAGGGGCGCTGCTC50.11649580615097856No Hit
TCGAGAAGCTCCGAGCGCGCA50.11649580615097856No Hit
AGGAGGGGGATCCGGTACTGTC50.11649580615097856No Hit
ATCCGCCGCGATCTCCTCCTC50.11649580615097856No Hit
AGGATCCTCGATCGCCTGCACGCC50.11649580615097856No Hit
TAAACCGTCTCGGAGTGAAGA50.11649580615097856No Hit
GACACCTCCCCCAGCTCCTTG50.11649580615097856No Hit
CGGGGCATAGCGAGCACGGGT50.11649580615097856No Hit
TCTCCGCTCGTCGTGCCCCTC50.11649580615097856No Hit
CGAGACCGGACTCCAAGCGAG50.11649580615097856No Hit
TCTTTCGGTCCCGCTGCTATC50.11649580615097856No Hit
AAGGTCGACGCGGGGCTCCGC50.11649580615097856No Hit
CGGAGACCCCCCACTTCAGCA50.11649580615097856No Hit
GAGGGTCGGGGCGTTGGCGGC50.11649580615097856No Hit
TTCCCGTTCGGGTCTCGGTCC50.11649580615097856No Hit
CAGCAGCGCCGCCTGGTCCGC50.11649580615097856No Hit
CCCCGGAGACTCCTGACGAC50.11649580615097856No Hit
TTGGCGGCCCCTTTCCCACGA50.11649580615097856No Hit
TTCGCCTCGCCGCCGGTCGCG50.11649580615097856No Hit
CACGGGGATGTCGAGGAGCGT50.11649580615097856No Hit
GAACCTCGCAGCGGATCCGGC50.11649580615097856No Hit
TTCCGGATTGCGTCGGCCGCG50.11649580615097856No Hit
CGCGGCGACTGTTCGGAGAGC50.11649580615097856No Hit
AAAGGTCGACGCGGGGCTCCG50.11649580615097856No Hit
GCGACGTCTCTGCGGCCCGAA50.11649580615097856No Hit
AGAGGGGGATCCGGTACTGTC50.11649580615097856No Hit
TGGTCAGAGGGCCGTCTGGGC50.11649580615097856No Hit
GACGAGAAGGATCCAAGGAC50.11649580615097856No Hit
CGGACGGACCAGGGAGGAGGT50.11649580615097856No Hit
CAGCAGCTGTTCCGGCACCA50.11649580615097856No Hit
AGCGCTCTTCGTATGACGGC50.11649580615097856No Hit
TCGGAGATCCGGGACAGCCT50.11649580615097856No Hit
CGGAGATCGCGGCACTCGGC50.11649580615097856No Hit
TCCTACGACCTCCACGTGCGT50.11649580615097856No Hit
TGTCCTGGTCATGCCCTCTCT50.11649580615097856No Hit
CTCCGGAGGACGGACGACCCC50.11649580615097856No Hit
TCCGGGTAAACGCAGTCGAC50.11649580615097856No Hit
ACGGGTCTCGGAAGGGCGGGT50.11649580615097856No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)