FastQCFastQC Report
Mon 30 Nov 2020
S34.raw.14987.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.raw.14987.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16844
Sequences flagged as poor quality0
Sequence length11-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGGACCGAGACCCGAACGG1861.1042507717881738No Hit
ATCTGTCGAGACAGGACGAACGAGCGATGGCGGTGGGTATCCT1450.8608406554262645No Hit
TGGCCACCGCGGACCTCGCCT1150.6827356922346236No Hit
CCGTCTGGTCCTCCGAAGCCT970.575872714319639No Hit
CGTGGTGCCGGAACAGCTGCTGGACC830.4927570648302066No Hit
TCCTATCGATCGCCTGACGCC780.4630729042982664No Hit
GGGGTCATGGCTGAACCACGACTTGGTAGAACGGGC770.4571360721918784No Hit
TCGGGCCGCAGAGACGTCGCC630.37402042270244595No Hit
GAAGTCCTATCGATCGCCTGACGCCTTCGTCCTCGCAGAGAAGA620.36808359059605794No Hit
CCTATCGATCGCCTGACGCCT610.3621467584896699No Hit
GGCGATCGAGGATCCTGGGGC600.35620992638328186No Hit
CCTGGCCACCGCGGAC520.30871526953217765No Hit
ATCTGTCGAGACAGGACGAACGAGCGATGGCGGTGGGT520.30871526953217765No Hit
GGGGTCATGGCTGAACCACGACTTGGTAGAACGGGCAGTT510.3027784374257896No Hit
CGAGGCTGTCCCGGATCTCCG500.29684160531940157No Hit
GGGGGTCGTCCGTC500.29684160531940157No Hit
ACTCGGACACGATTGGGTCCCACTTCGTGGTGCCGGAACAGCTGCTGGACC480.28496794110662554No Hit
CGGGGGTCGTCCGTC480.28496794110662554No Hit
CGGACACGATTGGGTCCCACTTCGTGGTGCCGGAACAGCTGCTGGACC460.27309427689384946No Hit
CTTCCGTCTGGTCCTCCGAAGCCT450.2671574447874614No Hit
ATCTGTCGAGACAGGACGAACGAGCGATGGCGGTGGGTATCCTTCGCTTGCGCGAATGGATCTCCA440.2612206126810734No Hit
TGAGGACCCCGCTGCTCTTCGAGCGACTGGTCGGGCGGACAGTGGCCCCT420.24934694846829733No Hit
TACAACCTGGCCACCGCGGAC360.21372595582996914No Hit
CGTGCGTGACACCTTCCGGTTGGCCGGCCTGG360.21372595582996914No Hit
ACTGGGGCATCGGTCAGGCGA360.21372595582996914No Hit
ATGTGGCTCTCCGAACAGTCGCCGCGGAGGCACGCGCCGAGGGCGGCCATCGGCTTCCGGTGCGCGCGGCCGCGG350.2077891237235811No Hit
CGAGGACCCCGC350.2077891237235811No Hit
GGCACGCACGTGGAGGTCGTAGGAGA350.2077891237235811No Hit
CGATGTGGCTCTCCGAACAGTCGCCGCGGAGGCACGCGCCGAGGGCGGCC330.19591545951080502No Hit
ACCTGGCCACCGCGGAC300.17810496319164093No Hit
CTGGTATACGGAGGGCCCCTC300.17810496319164093No Hit
CGGAACAGCTGCTGGACC290.17216813108525292No Hit
GAAGTCCTATCGATCGCCTGACGCCTTCGTCCTCGCAGA290.17216813108525292No Hit
GCCCACTGGGGTGTCGCGGCC290.17216813108525292No Hit
CTTATTACGGCCCTTTGGCGTGGTCTCTGTGACGGCCTCAAGGTCGGCGT280.16623129897886488No Hit
ATCGAGGATCCTGGGGCACG280.16623129897886488No Hit
TCGTGGTGCCGGAACAGCTGCTGGACC280.16623129897886488No Hit
CTCCGGCGGGTATGGGACTCCTTCC270.16029446687247684No Hit
AGGTCGGCGTGCGTGACACCTTCCGGTTGGCC270.16029446687247684No Hit
GTGGTGCCGGAACAGCTGCTGGACC270.16029446687247684No Hit
ATTATTACTGGGCCCGCTTCTGGACCGAGACCCGAACGGGAACCACTCGG270.16029446687247684No Hit
CTGGACCGAGACCCGAACGGG260.15435763476608882No Hit
CTGCCCACTGGGGTGTCGCGGCC260.15435763476608882No Hit
CCTGGCCACCGCGGACCTCGCCT260.15435763476608882No Hit
AGTCGCTCGGGTCTGTCTCTTGCTGAG250.14842080265970078No Hit
CTGACGACGCCGTCATACGAAGAGCGCTCGCCAAGCACTCGGACACGATT250.14842080265970078No Hit
CCGGTCCGTCCGACGAAGGGC240.14248397055331277No Hit
TGGCCACCGCGGAC240.14248397055331277No Hit
TTCTCCGGTCTCTGTCGCGCC240.14248397055331277No Hit
TTCGATGACGAGCGACTGGGCGGCGCCC230.13654713844692473No Hit
AAGGTGGACTCCGACCCGTGG230.13654713844692473No Hit
AACGGAAGGCGCTCTGGGATT230.13654713844692473No Hit
CCCACTTCGTGGTGCCGGAACAGCTGCTGGACCA220.1306103063405367No Hit
TGCCGGAACAGCTGCTGGACC220.1306103063405367No Hit
CTCGATCTGTCGAGACAGGACGAACGAGCGATGGCGGTGGGTATCCT220.1306103063405367No Hit
CTGGCCACCGCGGACCTCGCCT220.1306103063405367No Hit
ACGAGCGACTGGGCGGCGCCC210.12467347423414867No Hit
CGTCTGGTCCTCCGAAGCCT210.12467347423414867No Hit
TGCGCGCCGTCTGTTCCTCGC210.12467347423414867No Hit
GCCGGAACAGCTGCTGGACC200.11873664212776064No Hit
CGTGGTGCCGGAACAGCTGCTGGACCA200.11873664212776064No Hit
CTCGTCGTCCGCCGCTGTGGC200.11873664212776064No Hit
CTGCCCCCGGCGATGGCGGATGTGCTGTACTCTGCTGAGGAGGAGAGGTC200.11873664212776064No Hit
CGACCGCTGTCCCGATGGAGG200.11873664212776064No Hit
ACCCCGCTGCTCTTCGAGCGACTGGTCGGGCGGACAGTGGCCCCTCTCGGCACGCACGTGGAGGTCGTAGG200.11873664212776064No Hit
GGCACGCACGTGGAGGTCGTAGGA200.11873664212776064No Hit
AGTAATCCCCCGCGTCCGGCG190.1127998100213726No Hit
GGGATTCGTCGGCGTGCAGGC190.1127998100213726No Hit
CTGCGCGCCGTCTGTTCCTCGCACAGCTCGCTCGCCTCGGTC190.1127998100213726No Hit
GAGGACCCCGCTGCTCTTCGAGCGACTGGTCGGGCGGACAGTGGCCCCTCTCGGCACGCACGTGGAGGTCGTAGG190.1127998100213726No Hit
ATCTGTCGAGACAGGACGAACGAGCGATGGCGGTGGGTATCCTTCG190.1127998100213726No Hit
AAGGTCGGCGTGCGTGACACCTTCCGGTTGGCC180.10686297791498457No Hit
AACCTGGCCACCGCGGAC180.10686297791498457No Hit
CACTCGTGCTCAGAGGGGCTGTATGATGGGCCTCAATCTGTCTTGGGTCA180.10686297791498457No Hit
ACAACGGAAGGCGCTCTGGGA180.10686297791498457No Hit
AGGTGGACTCCGACCCGTGGA180.10686297791498457No Hit
CAGGACGAACGAGCGATGGCGGTGGGTATCCT180.10686297791498457No Hit
ATCCTTCTCGTCGTCCGCCGCTGTGGC180.10686297791498457No Hit
ACCGCGGACCTCGCCTGTGTC180.10686297791498457No Hit
CTACAACCTGGCCACCGCGGAC180.10686297791498457No Hit
TCGGCGTGCGTGACACCTTCCGGTTGGCCGGCCTGG180.10686297791498457No Hit
GAGGAGGAGATCGCGGCGGAT170.10092614580859652No Hit
ACAGCTCGCTCGCCTCGGTC170.10092614580859652No Hit
CAACGGAAGGCGCTCTGGGAT170.10092614580859652No Hit
CTCGGACACGATTGGGTCCCACTTCGTGGTGCCGGAACAGCTGCTGGACC170.10092614580859652No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)