FastQCFastQC Report
Mon 30 Nov 2020
S34.raw.14987.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.raw.14987.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4658
Sequences flagged as poor quality0
Sequence length11-76
%GC67

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGAGATCTCCGGAGGACGG2725.839416058394161No Hit
TCCCCGGAGACTCCTGACGAC1543.3061399742378708No Hit
GGAGATCGCGGCACTCGGCCT1312.812365822241305No Hit
CGAGGGTCGGG1292.769428939458995No Hit
CGAGAAGGATCCAAGGACGGA1212.5976814083297555No Hit
TTCCCCGGAGACTCCTGACGA1082.31859167024474No Hit
ACGAGAAGGATCCAAGGACGG661.4169171318162301No Hit
TCCTCGATCGCCTGCACGCCG631.3525118076427651No Hit
GAGTACATGACCCCACCGGCG511.094890510948905No Hit
TCGGGCGAGAAGGCTGCGGCC410.8802060970373551No Hit
CCGAGGGTCGGG360.7728638900815801No Hit
TCCGGCGACGTCTCTGCGGCC350.7513954486904251No Hit
CATCGGGATCCTCGTCGACGG310.6655216831258051No Hit
TAAGGGCCCGCGGATCACGAG300.6440532417346501No Hit
TCATCCAGCTCAGGACGCGCC270.579647917561185No Hit
CGACGAGAAGGATCCAAGGACC240.51524259338772No Hit
CGCATCGGGATCCTCGTCGAC240.51524259338772No Hit
CCGGAGATCGCGGCACTCGGC220.47230571060541005No Hit
CGACGTCTCTGCGGCCCGAAA220.47230571060541005No Hit
CGGAGATCGCGGCACTCGGCC220.47230571060541005No Hit
TGTAAGGGCCCGCGGATCACG220.47230571060541005No Hit
CCCAGGATCCTCGATCGCCT210.4508372692142551No Hit
CTCGGAGATCCGGGACAGCCT200.42936882782310004No Hit
TCCGCTCGTCGTGCCCCTCGG200.42936882782310004No Hit
AGCACGAGTGGTCAGAGGGCC190.40790038643194504No Hit
CCGAGAGATCTCCGGAGGACG180.38643194504079004No Hit
CCGGATTGCGTCGGCCGCGAC180.38643194504079004No Hit
GCACATCCGCCATCGCCGGGG170.36496350364963503No Hit
CGGAGACTCCTGACGACATGG170.36496350364963503No Hit
CGGGGTCCTCAGCTGAAGCGC170.36496350364963503No Hit
TGCTTGGCGAGCGCTCTTCGT170.36496350364963503No Hit
TCCCCGGAGACTCCTGACGACA170.36496350364963503No Hit
CGGAGATCCGGGACAGCCTCG160.34349506225848003No Hit
AGGACTTCGCGCGGTTCGCCT160.34349506225848003No Hit
TGTCTCCGCTCGTCGTGCCCC140.30055817947617003No Hit
ATCGAGACCGGACTCCAAGCG140.30055817947617003No Hit
CACGTGCCCCAGGATCCTCGA140.30055817947617003No Hit
ACATCGGCCACCACCTGGAGG140.30055817947617003No Hit
AGGATCCTCGATCGCCTGCAC130.279089738085015No Hit
TACAGCCCCTCTGAGCACGAG130.279089738085015No Hit
TTCCCCGGAGACTCCTGACGAC130.279089738085015No Hit
CAAGGACGGACGCCCGAGTGG130.279089738085015No Hit
AAGGGCCCGCGGATCACGAGC130.279089738085015No Hit
GACGCCCGAGTGGTTCCCGTT130.279089738085015No Hit
ATCGGGATCCTCGTCGACGGT120.25762129669386No Hit
CGACGAGAAGGATCCAAGGAC120.25762129669386No Hit
GATCGAGACCGGACTCCAAGC120.25762129669386No Hit
TCCCCCCCACGTGCCCCAGGA120.25762129669386No Hit
TCGGGGCATAGCGAGCACGGG110.23615285530270502No Hit
CTCGGCCTGAGTGGACGCGCC110.23615285530270502No Hit
AGAAGCTCCGAGCGCGCACGG100.21468441391155002No Hit
TCCAAGAACGCCGTGCGAGAC100.21468441391155002No Hit
GCATCGGGATCCTCGTCGACG100.21468441391155002No Hit
GAAGCCAAGAGTGTATCCCGC100.21468441391155002No Hit
AAGCCAAGAGTGTATCCCGCC100.21468441391155002No Hit
AATGGCCTTCCGGATTGCGTC100.21468441391155002No Hit
ATCCTCGATCGCCTGCACGCC100.21468441391155002No Hit
ATCCGGGTAAACGCAGTCGAC100.21468441391155002No Hit
CCAGGATCCTCGATCGCCT100.21468441391155002No Hit
TCGGCCTGAGTGGACGCGCCC100.21468441391155002No Hit
TAAGGTCCGTAGGCATGAGGT90.19321597252039502No Hit
TCCGGCACCACGAAGTGGGAC90.19321597252039502No Hit
TCGGTCCCGCTGCTATCCACC90.19321597252039502No Hit
CAATATTTCCAGGGGCGCCGC90.19321597252039502No Hit
CTCGATCGCCTGCACGCCGAC90.19321597252039502No Hit
AAGGATCCAAGGACGGACGCC90.19321597252039502No Hit
CCCACGAGACACCTCCCCCAG90.19321597252039502No Hit
TTTCCCACGAGACACCTCCCC90.19321597252039502No Hit
CGATAGCCTCCTCCATCGGGA90.19321597252039502No Hit
AAGACGTTCGAGCTCCTCGGA90.19321597252039502No Hit
ATCCAAGGACGGACGCCCGAG90.19321597252039502No Hit
TACCAGGTTCCCTACCCTCCC90.19321597252039502No Hit
CGCACGGGTTCGCTCTGGGTC90.19321597252039502No Hit
TAGGACTTCGCGCGGTTCGCCT90.19321597252039502No Hit
GGTCCGTAGGCATGAGGTCTG90.19321597252039502No Hit
TCGGGATCCTCGTCGACGGTG90.19321597252039502No Hit
AACCTCGCAGCGGATCCGGCG90.19321597252039502No Hit
CCCACGTGCCCCAGGATCCTC80.17174753112924002No Hit
TTCGGAGGACCAGACGGAAGG80.17174753112924002No Hit
ATCCGGCGACGTCTCTGCGGC80.17174753112924002No Hit
CCCGGAGACTCCTGACGACAT80.17174753112924002No Hit
TCCCGAAACTCCCCATCACCC80.17174753112924002No Hit
TACCCACCGCCATCGCTCGTT80.17174753112924002No Hit
TCTTGCCGCGCCTCTGACGGG80.17174753112924002No Hit
CAGCAAGAGACAGACCCGAGC80.17174753112924002No Hit
CCACGAGACACCTCCCCCAGC80.17174753112924002No Hit
AGGAACAGACGGCGCGCAGGG80.17174753112924002No Hit
GTCTCCGCTCGTCGTGCCCCT80.17174753112924002No Hit
AGCGATCTGCTCGGGCGAGAA80.17174753112924002No Hit
TTGTCTCCGCTCGTCGTGCCC80.17174753112924002No Hit
CCCCAGGATCCTCGATCGCCT80.17174753112924002No Hit
AGTGGACGCGCCCAGCGGGCA80.17174753112924002No Hit
GGCAGCCCTTCGTCGGACGGA80.17174753112924002No Hit
ATTCCTCCCAAGCTTCACCCA80.17174753112924002No Hit
TCCGGATTGCGTCGGCCGCGA80.17174753112924002No Hit
CGCGAGATCTCCGGAGGACGG70.15027908973808501No Hit
CGAGTGGTTCCCGTTCGGGTC70.15027908973808501No Hit
AGGAGTCCCATACCCGCCGGA70.15027908973808501No Hit
TCACGCACGCCGACCTTGAGG70.15027908973808501No Hit
TCGTGCGTGCCG70.15027908973808501No Hit
TTGTCCGCGAAGGGGGCCTGG70.15027908973808501No Hit
TCCACGGGTCGGAGTCCACCT70.15027908973808501No Hit
GGATCCTCGATCGCCTGCAC70.15027908973808501No Hit
TCCAGCAGCTGTTCCGGCACC70.15027908973808501No Hit
CACGGGTCTCGGAAGGGCGGG70.15027908973808501No Hit
ACGAGACACCTCCCCCAGCTC70.15027908973808501No Hit
CCAAGAACGCCGTGCGAGACA70.15027908973808501No Hit
GGGGCCAGGCCCACCGTGCGG70.15027908973808501No Hit
GAGACCGGACTCCAAGCGAGG60.12881064834693No Hit
ACACCTCCCCCAGCTCCTTGC60.12881064834693No Hit
CGAAAGGTCGACGCGGGGCTC60.12881064834693No Hit
CACCGGAAGCCGATGGCCGCC60.12881064834693No Hit
GCGACGTCTCTGCGGCCCGAAA60.12881064834693No Hit
AAGAGACAGACCCGAGCGACT60.12881064834693No Hit
ATACCCACCGCCATCGCTCGT60.12881064834693No Hit
TCCGATCTACGGGCGGCCGAG60.12881064834693No Hit
TCGGAGATCCGGGACAGCCTC60.12881064834693No Hit
CGAGGAGTACCTGGCCCACCA60.12881064834693No Hit
GGTTCGCTCTGGGTCGTCGCC60.12881064834693No Hit
GTACATGACCCCACCGGCGCT60.12881064834693No Hit
CTCTTGTTCTTGCCGCGCCTC60.12881064834693No Hit
ATGAGGTCTGTGGCGGCAGTC60.12881064834693No Hit
GGTCCCGCTGCTATCCACCGA60.12881064834693No Hit
CCCGGAGACTCCTGACGACA60.12881064834693No Hit
CACGGGGGACCAGCAGCGCCG60.12881064834693No Hit
CCACCGCCATCGCTCGTTCGT50.10734220695577501No Hit
CCTTGAGGCCGTCACAGAGAC50.10734220695577501No Hit
TTCGAGAAGCTCCGAGCGCGC50.10734220695577501No Hit
AATATTTCCAGGGGCGCCGCC50.10734220695577501No Hit
AGGGGTCAAGGGGCGCTGCTC50.10734220695577501No Hit
TCGAGAAGCTCCGAGCGCGCA50.10734220695577501No Hit
CGTGCGTGCCG50.10734220695577501No Hit
AGGAGGGGGATCCGGTACTGTC50.10734220695577501No Hit
ATCCGCCGCGATCTCCTCCTC50.10734220695577501No Hit
AGGATCCTCGATCGCCTGCACGCC50.10734220695577501No Hit
TAAACCGTCTCGGAGTGAAGA50.10734220695577501No Hit
CGGGGCATAGCGAGCACGGGT50.10734220695577501No Hit
TCTCCGCTCGTCGTGCCCCTC50.10734220695577501No Hit
CGAGACCGGACTCCAAGCGAG50.10734220695577501No Hit
TCTTTCGGTCCCGCTGCTATC50.10734220695577501No Hit
AAGGTCGACGCGGGGCTCCGC50.10734220695577501No Hit
CTCGAAGAGCAGCGGGGTCCT50.10734220695577501No Hit
CGGAGACCCCCCACTTCAGCA50.10734220695577501No Hit
CGAGAAGGATCCAAGGACGG50.10734220695577501No Hit
GAGGGTCGGGGCGTTGGCGGC50.10734220695577501No Hit
TTCCCGTTCGGGTCTCGGTCC50.10734220695577501No Hit
CAGCAGCGCCGCCTGGTCCGC50.10734220695577501No Hit
CCCCGGAGACTCCTGACGAC50.10734220695577501No Hit
TTGGCGGCCCCTTTCCCACGA50.10734220695577501No Hit
TTCGCCTCGCCGCCGGTCGCG50.10734220695577501No Hit
CACGGGGATGTCGAGGAGCGT50.10734220695577501No Hit
GAACCTCGCAGCGGATCCGGC50.10734220695577501No Hit
TTCCGGATTGCGTCGGCCGCG50.10734220695577501No Hit
CGCGGCGACTGTTCGGAGAGC50.10734220695577501No Hit
AAAGGTCGACGCGGGGCTCCG50.10734220695577501No Hit
GCGACGTCTCTGCGGCCCGAA50.10734220695577501No Hit
AGAGGGGGATCCGGTACTGTC50.10734220695577501No Hit
TGGTCAGAGGGCCGTCTGGGC50.10734220695577501No Hit
GACGAGAAGGATCCAAGGAC50.10734220695577501No Hit
CGGACGGACCAGGGAGGAGGT50.10734220695577501No Hit
CAGCAGCTGTTCCGGCACCA50.10734220695577501No Hit
AGCGCTCTTCGTATGACGGC50.10734220695577501No Hit
TCGGAGATCCGGGACAGCCT50.10734220695577501No Hit
CCCGCGGAGGCGAGGGCTCGC50.10734220695577501No Hit
CGGAGATCGCGGCACTCGGC50.10734220695577501No Hit
TCCTACGACCTCCACGTGCGT50.10734220695577501No Hit
TGTCCTGGTCATGCCCTCTCT50.10734220695577501No Hit
CTCCGGAGGACGGACGACCCC50.10734220695577501No Hit
CCGAGAGATCTCCGGAGGACGG50.10734220695577501No Hit
GAGAGATCTCCGGAGGACGG50.10734220695577501No Hit
TCCGGGTAAACGCAGTCGAC50.10734220695577501No Hit
GTGACCGCCACAGCGGCGGACGACGAGAAGGATCCAAGGACGGAC50.10734220695577501No Hit
ACGGGTCTCGGAAGGGCGGGT50.10734220695577501No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)