FastQCFastQC Report
Wed 31 Mar 2021
S33.15-35nt.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5636634
Sequences flagged as poor quality0
Sequence length15-35
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGACCTCGGGCTGTACGGGACTACCCGG2144923.80532069316546No Hit
CTCCGGGCGGGACCTCGAATCGTC1018211.80641496325644No Hit
AATCACGGAATTCTTGAACCAAGCGCAAGCGG782631.3884704949798055No Hit
TAGACTGTACCCCCTCTGGTCTAATGGACCGC758001.3447742038954456No Hit
ATCACGGAATTCTTGAACCAAGCGCAAGCGG703761.2485465616536395No Hit
GCATCGTTGGTTCAGTGGTAGAATGCTCGCCT691511.2268137331606062No Hit
TCCTCGGTAGTATAGTGGTCAGTATCCCCGCCT648761.150970596991041No Hit
TAGACTGTACCCCCTCTGGTCTAATGGACCGCT390920.6935344746527804No Hit
TAGACTGTACCCCCCCTGGTCTAATGGACCGT364360.6464141542629874No Hit
ATCACGGAATTCTTGAACCAAGCGCAAGCGGGTCG307140.544899668844917No Hit
AATCACGGAATTCTTGAACCAAGCGCAAGCG302830.5372532614322661No Hit
ATCACGGAATTCTTGAACCAAGCGCAAGCG295620.5244619395192237No Hit
GGGGCTGATTTTGGATTCGACGAAATTT294220.5219781876914485No Hit
CTCGGTAGTATAGTGGTCAGTATCCCCGCCT275640.48901525271997437No Hit
GCATCGTTGGTTCAGTGGTAGAATGCTCGCC238200.4225926324114711No Hit
AACGACCTCGGGCTGTACGGGACTACCCGG231790.41122059725715737No Hit
AGGAACGCTTTTATTAGATCAAAACCCATCGGCGC230750.40937552447081005No Hit
GCCTTCACGGGCGTCGCGTCGCGGTCCG198310.3518234464043612No Hit
AATCACGGAATTCTTGAACCAAGCGCAAGCGGG186410.3307115558682717No Hit
CGGGGTCGTGGGTTCGAGCCCCACGTTGGGCGCCA180150.31960563698121963No Hit
AATCACGGAATTCTTGAACCAAGCGCAAGCGGGT166790.29590354811045033No Hit
GGGGACAGAACTAGGAAGCAACTTAAAAGC165480.29357946604303203No Hit
ATCACGGAATTCTTGAACCAAGCGCAAGCGGG165300.2932601265223181No Hit
CTACGAGCCGGGCCTGTGCGAGGTGAAGTT151090.26805004547039957No Hit
CGCCGCGCTTCGGACCCCAGCGTG148170.26286964880103975No Hit
CGGAATTCTTGAACCAAGCGCAAGCGGGTCGCCG145530.25818600249723506No Hit
ATCACGGAATTCTTGAACCAAGCGCAAGC143380.25437166933315164No Hit
CTCGGCGGCGCGGCGGTGCTCGCGGGTCGCGTC142680.25312979341926406No Hit
AACATCGACATGTCGAACGCACATTGCTGGCCCGA127120.2255246659619908No Hit
CCTCGGCGGCGCGGCGGTGCTCGCGGGTCGCGTC125080.2219054847272326No Hit
GTGCCTTCACGGGCGTCGCGTCGCGGTCCG124570.22100068941854306No Hit
ACGTTTCGGGTCACGTACATCCGAGGGTCGGT120560.2138865145404154No Hit
TCCTCGTAGTATAGTGGTCAGTATCCCCGCCT115210.20439503434141723No Hit
GGTTCGCGCGCGTTTCTGCGGCGTATTGCGGC115040.20409343590518741No Hit
GTCAGCGAAATGTCCTGCCGGTGGCGGTTCG113300.20100648720495243No Hit
CTCCGGGCGGGACCTCGAATCGTCC113120.20068714768423848No Hit
CACGGAATTCTTGAACCAAGCGCAAGCGGGTCGCC111600.19799050284265396No Hit
AATCACGGAATTCTTGAACCAAGCGCAAGC110980.1968905556046392No Hit
CGTGCGGGAGGAACGCTTTTATTAGATCAAAACCC110100.19532934017003767No Hit
CGAGCCGGGCCTGTGCGAGGTGAAGTT108690.19282784725777832No Hit
CGGAATTCTTGAACCAAGCGCAAGCGGGTCGCC106450.18885384433333796No Hit
GGGGGTATAGCTCAGTGGTAGAGCATTCGACTG104100.1846846894795724No Hit
ACATCGACATGTCGAACGCACATTGCTGGCCCGA96160.17059826839919No Hit
CGGAATTCTTGAACCAAGCGCAAGCGGGTCGC93820.16644685462990855No Hit
ATCACGGAATTCTTGAACCAAGCGCAAGCGGGT93760.1663404081230039No Hit
GCATCGTTGGTTCAGTGGTAGAATGCTCGCCTGCC89210.15826821468273441No Hit
TCCTCGGTAGTATAGTGGTCAGTATCCCCGCC86240.1529991125909541No Hit
GGTCGGTATTTCCCGCGTCGGCGA85420.1515443436632572No Hit
ATCGACATGTCGAACGCACATTGCTGGCCCGA81280.14419953468683613No Hit
CTTTAACACTGAGGCGTGATGACGAGTC80380.14260283708326635No Hit
AAGGGTCCTGGGTTCGAGCCCCAGTTCGGGCGCCA77520.13752888692081125No Hit
ACGGAATTCTTGAACCAAGCGCAAGCGGGTCGCCG77470.1374401814983907No Hit
CTTCGCGGGCGTCAAACAGTTCGATGTTGGGA76370.13548866220513875No Hit
CAATCACGGAATTCTTGAACCAAGCGCAAGCGG76220.1352225459378771No Hit
AATTGAGTGTAGGCGACGACAATACCAGGAGTCCC76180.13515158159994067No Hit
AACACTGAGGCGTGATGACGAGTC75400.1337677770101802No Hit
CGTTTCGGGTCACGTACATCCGAGGGTCGGT73280.13000666709954914No Hit
GCGAAGATGGCGGAATCGGTAGACGCGCTAGC73190.12984699733919217No Hit
GAGGAACGCTTTTATTAGATCAAAACCCATCGGC72340.12833900515804292No Hit
TCCTCGGTAGTATAGTGGTCAGTATCCC71480.12681327189240954No Hit
ACGAGCCGGGCCTGTGCGAGGTGAAGTT70760.1255359138095537No Hit
AAGGAACTCCTCATACCCGTTGG69910.12402792162840447No Hit
TCCTCGGTAGTATAGTGGTCAGTATCCCCGCCTG69210.12278604571451686No Hit
GCCTCCGGGCGGGACCTCGAATCGTC65790.11671859482095166No Hit
CGCCGGTCGTGGCTGGGAACC60290.10696099835469182No Hit
CGGCGGCGCGGCGGTGCTCGCGGGTCGCGTC59170.10497399689247164No Hit
CACCCTCCGACACACGTTCCTGACCCTT59170.10497399689247164No Hit
AGGAACGCTTTTATTAGATCAAAACCCATCGGC58650.10405146049929799No Hit
CATCGACATGTCGAACGCACATTGCTGGCCCGAAA58120.10311118302164023No Hit
CGAGGCCAGGTGCGTCCTGTCGCGGTGCTG56930.10099999396803126No Hit
ACGGAATTCTTGAACCAAGCGCAAGCGGGTCGCC56460.10016616299727817No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)