FastQCFastQC Report
Wed 31 Mar 2021
S33.15-35nt.mivirus.NODE_8525.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.mivirus.NODE_8525.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length16-35
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTGATCAAGTAGGTATTTGTGTTTTCTCTGGC526.31578947368421No Hit
TAATATCCCAAAATGAAATGTCTACACTGTG210.526315789473683No Hit
TTCTATAGTATTTGGTGATCAAGTAGGT210.526315789473683No Hit
CTTTCGTGTATATTAGTGGCCGCCATGT15.263157894736842No Hit
TGGTGATCAAGTAGGTATTTGTGTTTTCTCTGGC15.263157894736842No Hit
AATTTCAATAATGCCCTGTGCCCTGGACG15.263157894736842No Hit
CTGTGATACAGGATGT15.263157894736842No Hit
AATTTCAATAATGCTCTGTGCCCTGGACGT15.263157894736842No Hit
CGCCTGTAGCGACGTTTGAGTAAGTCTCACA15.263157894736842No Hit
TACGGGAGATTTCACAACAAGTCATTGGAC15.263157894736842No Hit
TCGATGGAAATGGAGCCGCCT15.263157894736842No Hit
ATTTCAATAATGCTCTGTGCCCTGGACGT15.263157894736842No Hit
TTGGTGATCAAGTAGGTATTTGTGTTTTCTCT15.263157894736842No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)