FastQCFastQC Report
Wed 31 Mar 2021
S33.15-35nt.mivirus.NODE_9891.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.mivirus.NODE_9891.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190
Sequences flagged as poor quality0
Sequence length26-35
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTCTCCCACAAACGTCACCACGTGACTG126.315789473684211No Hit
TGAGGACGTCGAGTTAACTTTAATGTTGGT115.7894736842105265No Hit
TTGGTCTCCCACAAACGTCACCACGTGACT84.2105263157894735No Hit
TAAAGTTATCCAGGAACCATGTTAGGGC73.684210526315789No Hit
TCTACTCCTGAGCACTCTTTGTCCCCAATT73.684210526315789No Hit
TTATAAGCAACTGTTCTTACAACTCCCT52.631578947368421No Hit
TGGTCTCCCACAAACGTCACCACGTGACT52.631578947368421No Hit
TACTCCTGAGCACTCTTTGTCCCCAATTG52.631578947368421No Hit
TTGGATATGTTAGCTACGCCTTGGTACAAT42.1052631578947367No Hit
TGGTCTCCCACAAACGTCACCACGTGACTGT42.1052631578947367No Hit
TTGGTCTCCCACAAACGTCACCACGTGACTGT42.1052631578947367No Hit
TTGGATATGTTAGCTACGCCTTGGTACAA42.1052631578947367No Hit
TCAGAGAATGAGGACGTCGAGTTAACTT42.1052631578947367No Hit
CTAGACCCCGGTTAGTAGTGTACATGCCT42.1052631578947367No Hit
TCGGTATCTTGGATATGTTAGCTACGCCT42.1052631578947367No Hit
TCAAAGTCGGCCTTAAGGGTTAGAGGG42.1052631578947367No Hit
TAGCACCTAGATTCCTGATTACCCACTCCC42.1052631578947367No Hit
TAGCATCTACTCCTGAGCACTCTTTGTCCA31.5789473684210527No Hit
TCAGAGAATGAGGACGTCGAGTTAACTTT31.5789473684210527No Hit
TTACCCACTCCCACCATCCGTACAACTTC31.5789473684210527No Hit
TGAGCACTCTTTGTCCCCAATTGTTCCAAG31.5789473684210527No Hit
TCCCCAATTGTTCCAAGATCGGTCTCAGAG31.5789473684210527No Hit
TTTGAATAACAGCCGTCCTTTCTGCAGGA21.0526315789473684No Hit
TTGTCCCCAATTGTTCCAAGATCGGTCTCA21.0526315789473684No Hit
TCGGAATCTCATCGTAGCAGCTATTTGTGG21.0526315789473684No Hit
TCTTGGATATGTTAGCTACGCCTTGGTAC21.0526315789473684No Hit
AATTGATGTCACACTGTATTGAGGGGGA21.0526315789473684No Hit
TACCCACTCCCACCATCCGTACAACTTCTT21.0526315789473684No Hit
TGTGCTTTGCGATAGTAGACCATAAACCT21.0526315789473684No Hit
TTTGCTATGTTCCTCTCCATGTACACGAAC21.0526315789473684No Hit
GGAATTGATGTCACACTGTATTGAGGGGG21.0526315789473684No Hit
CCCCAATTGTTCCAAGATCGGTCTCAGAG21.0526315789473684No Hit
TGGTCTCCCACAAACGTCACCACGTGACTG21.0526315789473684No Hit
TAACACTAGTAGATCCCATAGTATGGCTA21.0526315789473684No Hit
AAGTTATCCAGGAACCATGTTAGGGC21.0526315789473684No Hit
TCCCCATAAACCCAGGAGTCGTCAAAGTC21.0526315789473684No Hit
TGAGGACGTCGAGTTAACTTTAATGTTGG10.5263157894736842No Hit
TAAATTAGCATGGCTCACGAACGATCAA10.5263157894736842No Hit
TCAACAGTGACAACCACTCGATCATTCGGT10.5263157894736842No Hit
TCATCTCTAACTAACGAGGCACCGTGGG10.5263157894736842No Hit
TGTCCCCAATTGTTCCAAGATCGGTCTCA10.5263157894736842No Hit
GAAACTATGTCCCCGTAAACCCAGGAGTC10.5263157894736842No Hit
TCAAAAGATTTAGGCAAAGGTTTGGTCGG10.5263157894736842No Hit
CACACTGTATTGAGGGGGAAGGCTCGAT10.5263157894736842No Hit
TCAAAGTCGGCCTTAAGGGTTAGAGGGG10.5263157894736842No Hit
TCTCTAACTAACGAGGCACCGTGGGGTAAAAAAAA10.5263157894736842No Hit
CTCCGTGTAAACATCTGATACAAATCACCA10.5263157894736842No Hit
TCCTGAGCACTCTTTGTCCCCAATTGTTC10.5263157894736842No Hit
TCCCCTTTTTCATCGCTGTATATCCTACCT10.5263157894736842No Hit
TGGTCTCCCACAAACGTCACCACGTGAC10.5263157894736842No Hit
TAGTTCCTCAGTCACTTCTAATTTATAAGC10.5263157894736842No Hit
GTGCTTTGCGATAGTAGACCATAAACCT10.5263157894736842No Hit
TATCTCCGTGTAAACATCTGATACAAATCACC10.5263157894736842No Hit
TTATCCAGGAACCATGTTAGGGCCAAATC10.5263157894736842No Hit
ATTCAAAAGATTTAGGCAAAGGTTTGGTC10.5263157894736842No Hit
TACCCACTCCCACCATCCGTACAACTTCT10.5263157894736842No Hit
TGTCCCCATAAACCCAGGAGTCGTCAAAGTCG10.5263157894736842No Hit
TTGGTAACAACTAGACCCCGGTTAGTAGT10.5263157894736842No Hit
TATGGTTAACCCATTGTAAGCCGGCACCG10.5263157894736842No Hit
TTAAGTTATCCAGGAACCATGTTAGGGC10.5263157894736842No Hit
TAAAGTTATCCAGGAACCATGTTAGGAC10.5263157894736842No Hit
TTAACCCATTGTAAGCCGGCACCGCATGAGAAAAC10.5263157894736842No Hit
TTCAGTTAAGTGACTTGGTCTCCCACAAAC10.5263157894736842No Hit
TAACCCATTGTAAGCCGGCACCGCATGAGAA10.5263157894736842No Hit
TCCGTGTAAACATCTGATACAAATCACCAG10.5263157894736842No Hit
GCTGTATATCCTACCTTTTTCGTGTATGT10.5263157894736842No Hit
TAGCACCTAGATTCCTGATTACCCACTCC10.5263157894736842No Hit
AGACCCCGGTTAGTAGTGTACATGCCTGTT10.5263157894736842No Hit
TGAAACTATGTCCCCATAAACCCAGGAGTC10.5263157894736842No Hit
TAGCATCTACTCCTGAGCACTCTTTGTCC10.5263157894736842No Hit
TTTGTCCCCAATTGTTCCAAGATCGGTCTC10.5263157894736842No Hit
TCTACTCCTGAGCACTCTTTTTCCCCAATT10.5263157894736842No Hit
TGACAACCACTCGATCATTCGGTATCTTG10.5263157894736842No Hit
TAGATTCCTGATTACCCACTCCCACCATC10.5263157894736842No Hit
ATAAATTAGCATGGCTCACGAACGATCAAC10.5263157894736842No Hit
TCATCTCTAACTAACGAGGCACCGTGGGG10.5263157894736842No Hit
TGTCCCCAATTGTTCCAAGATCGGTCTCAGAG10.5263157894736842No Hit
TGGTCTCCCACAAACGTCACCACGTGTCTG10.5263157894736842No Hit
TATGCTTTTCTGAAACTATGTCCCCATA10.5263157894736842No Hit
AATAAAGTTATCCAGGAACCATGTTAGGGC10.5263157894736842No Hit
TTCCAAGATCGGTCTCAGAGAATGAGGAC10.5263157894736842No Hit
TCACCAGTTCTGTTTGCTATGTTCCTCTCC10.5263157894736842No Hit
TAAAGTTATCCAGGAACCATGTTAGGGCCA10.5263157894736842No Hit
TGTCCCCAATTGTTCCAAGATCGGTCTCAGA10.5263157894736842No Hit
TGACAACCACTCGATCATTCGGTATCTTGG10.5263157894736842No Hit
CTACTCCTGAGCACTCTTTGTCCCCAATT10.5263157894736842No Hit
TCCCCTTTTTCATCGCTGTATATCCTACCTT10.5263157894736842No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)