FastQCFastQC Report
Wed 31 Mar 2021
S33.15-35nt.orbivirus.NODE_20583.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.orbivirus.NODE_20583.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1441
Sequences flagged as poor quality0
Sequence length15-35
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTGCAAACGGCGCGGATCAGATCAACTC745.135322692574601No Hit
TAGGCGGTCGGAACAAGGGTCAAAGTTGGC624.302567661346287No Hit
CCTGAGCCTGAGCCGGAGATCCAACTTAC584.02498265093685No Hit
TCTCGAGATCGTAGGCAAAGTCCCCGGCC382.63705759888966No Hit
TCCAGGTCTCATCGGCAAGACAACCAGCC372.5676613462873004No Hit
TAAACCAGTTGATAAGGCTCGTCGTGCCAAA312.1512838306731434No Hit
TCCTGAGCCTGAGCCGGAGATCCAACTTAC271.8736988202637055No Hit
TACAATATGTTCTCCACCCAAGCCAAGCCT261.8043025676613464No Hit
GCCAAGCCTGAGATCGAACACATTGAGAA201.3879250520471893No Hit
TTCTCGAGATCGTAGGCAAAGTCCCCGGCC201.3879250520471893No Hit
TCGAGATCGTAGGCAAAGTCCCCGGCCAC191.31852879944483No Hit
TTCTCCACCCAAGCCAAGCCTGAGATCGAAC181.2491325468424705No Hit
TGAGGGACTCACGCTCAGCACGAGAAA171.179736294240111No Hit
TTCCCACTAACTGAGACCTGGACGGTCACGGCCT161.1103400416377516No Hit
TCCGAACTCGAACTGGCCCCGGTCCTTTT151.040943789035392No Hit
TCAAAGTTGGCGTGGCGATGGGTCCAGGT130.9021512838306732No Hit
TGAGGGACTCACGCTCAGCACGAGAAAGA130.9021512838306732No Hit
CCTGAGCCTGAGCCGGAGATCCAACTTACT130.9021512838306732No Hit
TATCCCCGGATATCTTTGACGAAAGACTT120.8327550312283136No Hit
TACTATGCGCGGAAGAGCCTTGAGCAAGG120.8327550312283136No Hit
TTCCGATCTCTTAGTCTCTCCGTCGCCT120.8327550312283136No Hit
TCTCCACCCAAGCCAAGCCTGAGATCGAAC110.7633587786259541No Hit
AAGGACATCCACTTAAACCAGTTGATAAGGC110.7633587786259541No Hit
AGGAGAAGTAGGATCAACGGGTATGGGT100.6939625260235947No Hit
TGCATCTGTGAACCCAGGATTCGGGGGT100.6939625260235947No Hit
TAGTGCAAACGGCGCGGATCAGATCAACT100.6939625260235947No Hit
TTAAACCAGTTGATAAGGCTCGTCGTGCC100.6939625260235947No Hit
TATGAAAAGGAGAAGTAGGATCAACGGGT100.6939625260235947No Hit
TCGAGCGCCACATGCCGATGCATCTGGGAA80.5551700208188758No Hit
TACTATGCGCGGAAGAGCCTTGAGCAAG80.5551700208188758No Hit
TAGGCGGTCGGAACAAGGGTCAAAGTT80.5551700208188758No Hit
TCAACTTCTCGAGATCGTAGGCAAAGTCCCC80.5551700208188758No Hit
TCCACCCAAGCCAAGCCTGAGATCGAACAC80.5551700208188758No Hit
CCTGAGCCTGAGCCGGAGATCCAACTTA80.5551700208188758No Hit
TAAACCAGTTGATAAGGCTCGTCGTGCCAAAC70.4857737682165163No Hit
TGAAACGGATGAGGACTCAGCAGAGAGTT70.4857737682165163No Hit
TAGTCGATAGTCGAGGGGATGAAACGGAT70.4857737682165163No Hit
TCCGATTAGCGCGCCCGTACCCAGCAACTT70.4857737682165163No Hit
TATGAAAAGGAGAAGTAGGATCAACGGG70.4857737682165163No Hit
CCTGAGCCTGAGCCGGAGATCCAACTTAA70.4857737682165163No Hit
TCCTGAGCCTGAGCCGGAGATCCAACTTA60.4163775156141568No Hit
TTAGCGCGCCCGTACCCAGCAACTTTCATG60.4163775156141568No Hit
TTAGAGAGTAGATGAGGGACCTCCTGTT60.4163775156141568No Hit
AACCAGTTGATAAGGCTCGTCGTGCCAAA60.4163775156141568No Hit
TGGGAAGGCAATCGGAGAAGGAGGACATC60.4163775156141568No Hit
TGCACTGTCCACTGAGGATACGAATTCGCC60.4163775156141568No Hit
GACACAATGTCGTCGAGCACATCATAAAGG60.4163775156141568No Hit
AGCCAAGCCTGAGATCGAACACATTGAGAA50.3469812630117973No Hit
TGAGGGACTCACGCTCAGCACGAGAA50.3469812630117973No Hit
CACTGAGGATACGAATTCGCCCGAACCGG50.3469812630117973No Hit
TGAGGGACTCACGCTCAGCACGAGAAAG50.3469812630117973No Hit
TAGGTGGGAGCTAACTGCAACTTGAGCGT50.3469812630117973No Hit
TAGGCGGTCGGAACAAGGGTCAAAGTTGG50.3469812630117973No Hit
TGAAAAGAACTTACTCCCATGGTACGAACC50.3469812630117973No Hit
TGCAAACGGCGCGGATCAGATCAACTC50.3469812630117973No Hit
TCGGAACAAGGGTCAAAGTTGGCGTGG50.3469812630117973No Hit
TCCACTGAGGATACGAATTCGCCCGAACCGG50.3469812630117973No Hit
TTAGCGCGCCCGTACCCAGCAACTTTCATGAT50.3469812630117973No Hit
TCCACCCAAGCCAAGCCTGAGATCGAACACA50.3469812630117973No Hit
TCGAACTGGCCCCGGTCCTTTTAAAAACTC50.3469812630117973No Hit
TATGTCGTCCTGAGCCTGAGCCGGAGAT50.3469812630117973No Hit
TTAGGGCTTCCAATGAAGGACATCCACT40.2775850104094379No Hit
TTCTCAAGATGAGAACCTAGGCGGTCGGAAC40.2775850104094379No Hit
TCCAAAAGGTTAGGGCTTCCAATGAAGGAC40.2775850104094379No Hit
CTTATGTCGTCCTGAGCCTGAGCCGGAG40.2775850104094379No Hit
TGCAAACGGCGCGGATCAGATCAACTCCGTC40.2775850104094379No Hit
TCCACTTAAACCAGTTGATAAGGCTCGTCG40.2775850104094379No Hit
TGGGAAGCGGAAGGACAAATATGGTACGAG40.2775850104094379No Hit
TGCATCTGTGAACCCAGGATTCGGGGG40.2775850104094379No Hit
TCCACTGAGGATACGAATTCGCCCGAACCG40.2775850104094379No Hit
TGAGGGACTCACGCTCAGCACGAGAAAGAA40.2775850104094379No Hit
TAAACCAGTTGATAAGGCTCGTCGTGCCAA40.2775850104094379No Hit
TATTCTGCAGACGTCGGCCCATGTGGCGGC40.2775850104094379No Hit
AAACCAGTTGATAAGGCTCGTCGTGCCAAA40.2775850104094379No Hit
TTCCGAACTCGAACTGGCCCCGGTCCTTTT40.2775850104094379No Hit
TTTCCGATCTCTTAGTCTCTCCGTCGCCT40.2775850104094379No Hit
TGCACTGTCCACTGAGGATACGAATTCGCCCGAA40.2775850104094379No Hit
TCAACTTCTCGAGATCGTAGGCAAAGTCCC40.2775850104094379No Hit
TCCAGGTCTCATCGGCAAGACAACCAGC40.2775850104094379No Hit
TAGTCGATAGTCGAGGGGATGAAACGGA40.2775850104094379No Hit
TTCCGAACTCGAACTGGCCCCGGTCCTTT40.2775850104094379No Hit
TCCCCGGATATCTTTGACGAAAGACT30.2081887578070784No Hit
TGCATCTGGGAACCCAGGATTCGGGGG30.2081887578070784No Hit
TGTGGCGGCGCTAGCACTAGTGCAAACGGC30.2081887578070784No Hit
TATGCGCGGAAGAGCCTTGAGCAAG30.2081887578070784No Hit
TCGGAACATAGAACGAGGAGAGAAGACTGC30.2081887578070784No Hit
TGTCGTCCTGAGCCTGAGCCGGAGATCCAC30.2081887578070784No Hit
TCCGTCGCCTTCCGAACTCGAACTGGCCC30.2081887578070784No Hit
TGACGAAAGACTTGGGAGAGCACGACACA30.2081887578070784No Hit
TACTATGCGCGGAAGAGCCTTGAGCAAGGC30.2081887578070784No Hit
TATGGGAAGGCAATCGGAGAAGGAGGA30.2081887578070784No Hit
AAAGTTGGCGTGGCGATGGGTCCAGGT30.2081887578070784No Hit
TAGTCGAGCGCCACATGCCGATGCATCTG30.2081887578070784No Hit
CCTGAGCCTGAGCCGGAGATCCAACTT30.2081887578070784No Hit
TCTGATGTCGTCCGAAGATTGCAATGAAGC30.2081887578070784No Hit
TCTCTCCGTCGCCTTCCGAACTCGAACTG30.2081887578070784No Hit
TTGAGCGTGAATGGTGGGAAGCGGAAGGAT30.2081887578070784No Hit
TTAAACCAGTTGATAAGGCTCGTCGTGC30.2081887578070784No Hit
TCCTGAGCCTGAGCCGGAGATCCAACTT30.2081887578070784No Hit
TATTCTGCAGACGTCGGCCCATGTGGCGG30.2081887578070784No Hit
CCTGAGCCTGAGCCGGAGATCCAACTTAAA30.2081887578070784No Hit
TGAGGATACGAATTCGCCCGAACCGG30.2081887578070784No Hit
TCAGATCAACTCCGTCGACGGAAAGGAT30.2081887578070784No Hit
CGCGTATTCTGCAGACGTCGGCCCATG30.2081887578070784No Hit
TAGCCTTTGTCTATGTGTTCGCCCCTTC30.2081887578070784No Hit
TCGAACTGGCCCCGGTCCTTTTAAAAACT30.2081887578070784No Hit
AACCAGTTGATAAGGCTCGTCGTGCCAAAC30.2081887578070784No Hit
TGAGACCTGGACGGTCACGGCCTTGACACC30.2081887578070784No Hit
TAAACCAGTTGATAAGGCTCGTCGTGCC30.2081887578070784No Hit
TCGGAACAAGGGTCAAAGTTGGCGTGGCGC30.2081887578070784No Hit
TCGGAACAAGGGTCAAAGTTGGCGTGGCGAT30.2081887578070784No Hit
TCAACGGGTATGGGTTCAAGGAATTGAGT30.2081887578070784No Hit
TGCATCTGGGAACCCAGGATTCGGGGGTG30.2081887578070784No Hit
TAACTGAGACCTGGACGGTCACGGCCTTGAC30.2081887578070784No Hit
TCGGAACATAGAACGAGGAGAGAAGACT30.2081887578070784No Hit
TTCTCCACCCAAGCCAAGCCGGAGATCGAAC30.2081887578070784No Hit
TGATGTCGTCCGAAGATTGCAATGAAGC30.2081887578070784No Hit
TCGTCCTGAGCCTGAGCCGGAGATCCAACT30.2081887578070784No Hit
TTAAACCAGTTGATAAGGCTCGTCGTGCCAAA30.2081887578070784No Hit
TCACGAAGTCCCTCTCTGATGTCGTCCG30.2081887578070784No Hit
TTAGTCTCTCCGTCGCCTTCCGAACTCGA20.13879250520471895No Hit
CCACCCAAGCCAAGCCTGAGATCGAACACAT20.13879250520471895No Hit
TCCATAAAGGATCTGGGCTTTTCCGGCAAA20.13879250520471895No Hit
TGAAACGGATGAGGACTCAGCAGAGAGTTA20.13879250520471895No Hit
CACCCAAGCCAAGCCTGAGATCGAACACAT20.13879250520471895No Hit
CTGAGCCTGAGCCGGAGATCCAACTTACT20.13879250520471895No Hit
TTTGACGAAAGACTTGGGAGAGCACGACAC20.13879250520471895No Hit
TCAAAGGCCTCGTAACTTCCCTGCTCGACT20.13879250520471895No Hit
TTCACGAAGTCCCTCTCTGATGTCGTCCG20.13879250520471895No Hit
TTTGACGAAAGACTTGGGAGAGCACGACCCC20.13879250520471895No Hit
TGAGACCTGGACGGTCACGGCCTTGACACCCC20.13879250520471895No Hit
AAGGATCTGGGCTTTTCCGGCAAATTCCGGCC20.13879250520471895No Hit
TAGTGCAAACGGCGCGGATCAGATCAAC20.13879250520471895No Hit
TAGGATCAACGGGTATGGGTTCAAGGAAT20.13879250520471895No Hit
CCTGAGCCTGAGCCGGAGATCCAACTTACTA20.13879250520471895No Hit
TGAGTATGTATGGGAAGGCAATCGGAGAA20.13879250520471895No Hit
TCAAGATGAGAACCTAGGCGGTCGGAACA20.13879250520471895No Hit
TCGAGATCGTAGGCAAAGTCCCCGGCCACT20.13879250520471895No Hit
TCCGATCTCTTAGTCTCTCCGTCGCCTTCC20.13879250520471895No Hit
TCCACCCAAGCCAAGCCTGAGATCGAACACAT20.13879250520471895No Hit
TATGTCGTCCTGAGCCTGAGCCGGAGATCC20.13879250520471895No Hit
TTCGGGGGTGGTAAGCGAGAAGCCTGCGGC20.13879250520471895No Hit
TCTTTAACTTAGCTTTCTGAACCTTCCC20.13879250520471895No Hit
TGAAGGACATCCACTTAAACCAGTTGATA20.13879250520471895No Hit
TGAGCCGGAGATCCAACTTACTAGGTGGGA20.13879250520471895No Hit
TCCCTCTCTGATGTCGTCCGAAGATTGC20.13879250520471895No Hit
TCCCTCTCTGATGTCGTCCGAAGATTGCA20.13879250520471895No Hit
CACTAGTGCAAACGGCGCGGATCAGATC20.13879250520471895No Hit
CTTTACAATATGTTCTCCACCCAAGCCAAG20.13879250520471895No Hit
TAATCGAGCGCCACATGCCGATGCATCTG20.13879250520471895No Hit
CTCGAGATCGTAGGCAAAGTCCCCGGCCAC20.13879250520471895No Hit
TCCCCGGATATCTTTGACGAAAGACTTGG20.13879250520471895No Hit
TGAGGGACTCACGCTCAGCACGAGAAAGAC20.13879250520471895No Hit
TCAAAGTTGGCGTGGCGATGGGTCCAGG20.13879250520471895No Hit
TTCCGATCTCTTAGTCTCTCCGTCGCCTTC20.13879250520471895No Hit
TAGGGCTTCCAATGAAGGACATCCACT20.13879250520471895No Hit
TGCATCTGGGAACCCAGGATTCGGGGGTGG20.13879250520471895No Hit
TCGAGATCGTAGGCAAAGTCCCCGGCCACGC20.13879250520471895No Hit
TGCGGCAATGGCCTGGGAATGAGCCTGAGA20.13879250520471895No Hit
TAACTTAGCTTTCTGAACCTTCCCACTA20.13879250520471895No Hit
TTCTCCACCCAAGCCAAGCCTGAGATCGAACACAT20.13879250520471895No Hit
TCGGAACAAGGGTCAAAGTTGGCGTGGCG20.13879250520471895No Hit
TTAACTTAGCTTTCTGAACCTTCCCACTA20.13879250520471895No Hit
TGAAACGGATGAGGACTCAGCAGAGAGTTAGCC20.13879250520471895No Hit
CCCCCAACGGGACAACGCCTCCTCACCATC20.13879250520471895No Hit
CACTAACTGAGACCTGGACGGTCACGGCC20.13879250520471895No Hit
TATCCCCGGATATCTTTGACGAAAGACTTG20.13879250520471895No Hit
CGACTATCGTAACGAAAAGCGTTCCCTT20.13879250520471895No Hit
TATGTCGTCCTGAGCCTGAGCCGGAGA20.13879250520471895No Hit
TAGGCGGTCGGAACAAGGGTCAAAGTTGGCT20.13879250520471895No Hit
TAGGCGGTCGGAACAAGGGTCAAAGTTGGCG20.13879250520471895No Hit
CCTAGGCGGTCGGAACAAGGGTCAAAGTT20.13879250520471895No Hit
TAGGATCAACGGGTATGGGTTCAAGGAA20.13879250520471895No Hit
TGGTGGGAAGCGGAAGGACAAATATGGTAAA20.13879250520471895No Hit
CTTCCCACTAACTGAGACCTGGACGGTCACGG20.13879250520471895No Hit
AAACGGATGAGGACTCAGAAGAGAGTTAGC20.13879250520471895No Hit
TTCCCACTAACTGAGACCTGGACGGTCACG20.13879250520471895No Hit
CGGAAGGACAAATATGGTACGAGTGGCT20.13879250520471895No Hit
AGCCAAGCCTGAGATCGAACACATTGAGA20.13879250520471895No Hit
TGTCGTCCGAAGATTGCAATGAAGCAACTC20.13879250520471895No Hit
TATGTTCTCCACCCAAGCCAAGCCTGAGA20.13879250520471895No Hit
TGTCCGATTAGCGCGCCCGTACCCAGCAACT20.13879250520471895No Hit
AACGGGACAACGCCTCCTCACCATCAGAGC20.13879250520471895No Hit
TTATGTCGTCCTGAGCCTGAGCCGGAGAT20.13879250520471895No Hit
TGAGCCTGAGCCGGAGATCCAACTTACT20.13879250520471895No Hit
TCAAAGGCCTCGTAACTTCCCTGCTCGAC20.13879250520471895No Hit
TCTCGAGATCGTAGGCAAAGTC20.13879250520471895No Hit
TTAGCGCGCCCGTACCCAGCAACTTTCAT20.13879250520471895No Hit
AGGGCTTCCAATGAAGGACATCCACTTAAACCAG20.13879250520471895No Hit
TTTAACTTAGCTTTCTGAACCTTCCCACT20.13879250520471895No Hit
AAGGAGAAGTAGGATCAACGGGTATGGGT20.13879250520471895No Hit
TGTTCTCCACCCAAGCCAAGCCTGAGATC20.13879250520471895No Hit
CTTCCCACTAACTGAGACCTGGACGGTCACGGCCT20.13879250520471895No Hit
TCCCACTAACTGAGACCTGGACGGTCACGGCCT20.13879250520471895No Hit
TCAAAGTTGGCGTGGCGATGGGTCCAAAA20.13879250520471895No Hit
TATGTCGTCCTGAGCCTGAGCCGGAGAA20.13879250520471895No Hit
AGGAGAAGTAGGATCAACGGGTATGGGTTC20.13879250520471895No Hit
TAGCTTTCTGAACCTTCCCACTAACTGAG20.13879250520471895No Hit
TGAGCCTGAGCCGGAGATCCAACTTACTA20.13879250520471895No Hit
TCGAGCGCCACATGCCGATGCATCTGGGA20.13879250520471895No Hit
TGAGATCGAACACATTGAGAAGGCATTCAA20.13879250520471895No Hit
TGAAACGGATGAGGACTCAGAAGAGAGTT20.13879250520471895No Hit
TAGGATCAACGGGTATGGGTTCAAGGA20.13879250520471895No Hit
TCCTGAGCCTGAGCCGGAGATCCAACTTACT20.13879250520471895No Hit
TTGAAAAGAACTTACTCCCATGGTACGAACC20.13879250520471895No Hit
TGCGGCGATGGCCTGGGAATGAGCCTGAG20.13879250520471895No Hit
TCCGTCGACGGAAAGGATACTATGCGCGG20.13879250520471895No Hit
TAGCACGGAATTCCAAAAGGTTAGGGCTTC20.13879250520471895No Hit
TCTCGAGATCGTAGGCAAAGTCCCCGGC20.13879250520471895No Hit
AAGGAGAAGTAGGATCAACGGGTATGGG20.13879250520471895No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)