FastQCFastQC Report
Wed 31 Mar 2021
S33.15-35nt.orbivirus.NODE_5225.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.orbivirus.NODE_5225.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11
Sequences flagged as poor quality0
Sequence length27-33
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCACCTAGTAAGTTGGATCTCCGGCTCAG218.181818181818183No Hit
TTTTTACCTCCTCGTGACTCTTGTGTG218.181818181818183No Hit
AAAAAAAATGTAGGTAGGTACCTATCCTATCCT218.181818181818183No Hit
TATCCTTCCATTTTTACCTCCTCGTGACT19.090909090909092No Hit
CCAGCTCTCAACAACTTTTCCTTGTACT19.090909090909092No Hit
TTAATCGAATAGACAATATGACATGAGAGAA19.090909090909092No Hit
ATCCTTCCATTTTTACCTCCTCGTGACT19.090909090909092No Hit
CTCTGGTTCTGTCCTGCCTGGCGACACC19.090909090909092No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)