FastQCFastQC Report
Wed 31 Mar 2021
S33.15-35nt.orbivirus.NODE_5225.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.orbivirus.NODE_5225.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2079
Sequences flagged as poor quality0
Sequence length17-35
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTGCAAACGGCGCGGATCAGATCAACTC743.5594035594035596No Hit
TAGGCGGTCGGAACAAGGGTCAAAGTTGGC622.982202982202982No Hit
CCTGAGCCTGAGCCGGAGATCCAACTTAC582.7898027898027897No Hit
TCACGAGGAGGTAAAAATGGAAGGATAGG492.356902356902357No Hit
TCTCGAGATCGTAGGCAAAGTCCCCGGCC381.8278018278018278No Hit
TCGACAGGCAGGACAGAACCAGAGAGTCTCC381.8278018278018278No Hit
TCCAGGTCTCATCGGCAAGACAACCAGCC371.7797017797017798No Hit
TCGGAAGTTGTCCATCTTAGCGTGCTGGT361.7316017316017316No Hit
TAAACCAGTTGATAAGGCTCGTCGTGCCAAA311.491101491101491No Hit
TCAACTCCGTCGACGGAAACGATTTCCCCT311.491101491101491No Hit
TCCTGAGCCTGAGCCGGAGATCCAACTTAC271.2987012987012987No Hit
TACAATATGTTCTCCACCCAAGCCAAGCCT261.2506012506012507No Hit
TCAACTCCGTCGACGGAAACGATTTCCCC251.2025012025012025No Hit
GCCAAGCCTGAGATCGAACACATTGAGAA200.9620009620009621No Hit
TTCTCGAGATCGTAGGCAAAGTCCCCGGCC200.9620009620009621No Hit
TCGAGATCGTAGGCAAAGTCCCCGGCCAC190.9139009139009139No Hit
TTCTCCACCCAAGCCAAGCCTGAGATCGAAC180.8658008658008658No Hit
TGAGGGACTCACGCTCAGCACGAGAAA170.8177008177008177No Hit
TTCCCACTAACTGAGACCTGGACGGTCACGGCCT160.7696007696007696No Hit
TCAACCAGCTCGGAGAACGAGATACCGTCAGGC150.7215007215007215No Hit
TTATCCCACCAAGTGCCATAGACGAAACCCT140.6734006734006733No Hit
TCAAAGTTGGCGTGGCGATGGGTCCAGGT130.6253006253006254No Hit
TGAGGGACTCACGCTCAGCACGAGAAAGA130.6253006253006254No Hit
TCAACTCCGTCGACGGAAACGATTTCCCCTT130.6253006253006254No Hit
CCTGAGCCTGAGCCGGAGATCCAACTTACT130.6253006253006254No Hit
TATCCCCGGATATCTTTGACGAAAGACTT120.5772005772005772No Hit
TTATCCATTATTCTGACCCCCGAGGTAGAA120.5772005772005772No Hit
TCTCCACCCAAGCCAAGCCTGAGATCGAAC110.5291005291005291No Hit
TGGTATGAGAAACTTTATGCATGGCGCGC110.5291005291005291No Hit
TCAGTAAGGGGTACAGGGACGTCAGGGG110.5291005291005291No Hit
GGCTGGGTGTCGACAGGCAGGACAGAACC110.5291005291005291No Hit
AAGGACATCCACTTAAACCAGTTGATAAGGC110.5291005291005291No Hit
AGGAGAAGTAGGATCAACGGGTATGGGT100.48100048100048104No Hit
TGCATCTGTGAACCCAGGATTCGGGGGT100.48100048100048104No Hit
TTATCCATTATTCTGACCCCCGAGGTAGA100.48100048100048104No Hit
TACCCGGGTACTGCACAACACTGTCCAACAC100.48100048100048104No Hit
TAGTGCAAACGGCGCGGATCAGATCAACT100.48100048100048104No Hit
TGAACAAGGTCCAATGAGGAGACAGCTCTCGC100.48100048100048104No Hit
TTAAACCAGTTGATAAGGCTCGTCGTGCC100.48100048100048104No Hit
TATGAAAAGGAGAAGTAGGATCAACGGGT100.48100048100048104No Hit
TATGTACCCGGGTACTGCACAACACTGTCC90.4329004329004329No Hit
TCGACAGGCAGGACAGAACCAGAGAGTCTC90.4329004329004329No Hit
TCGGAAGTTGTCCATCTTAGCGTGCTGGTC90.4329004329004329No Hit
TCGAGCGCCACATGCCGATGCATCTGGGAA80.3848003848003848No Hit
TAGGCGGTCGGAACAAGGGTCAAAGTT80.3848003848003848No Hit
CCCGGGTACTGCACAACACTGTCCAACACC80.3848003848003848No Hit
TGCCCACTGTGCTTATGTTATCAAGGCGTG80.3848003848003848No Hit
TACAAGGAAAAGTTGTTGAGGGCTGGTG80.3848003848003848No Hit
TCAACTTCTCGAGATCGTAGGCAAAGTCCCC80.3848003848003848No Hit
TCCACCCAAGCCAAGCCTGAGATCGAACAC80.3848003848003848No Hit
CCTGAGCCTGAGCCGGAGATCCAACTTA80.3848003848003848No Hit
TAAACCAGTTGATAAGGCTCGTCGTGCCAAAC70.33670033670033667No Hit
TACAAGGAAAAGTTGTTGAGAGCTGGTG70.33670033670033667No Hit
TATCCCACCAAGTGCCATAGACGAAACCCT70.33670033670033667No Hit
TGTCGACAGGCAGGACAGAACCAGAGAGT70.33670033670033667No Hit
TTATCCATTATTCTGACCCCCGAGGTAG70.33670033670033667No Hit
TCCGATTAGCGCGCCCGTACCCAGCAACTT70.33670033670033667No Hit
TATGAAAAGGAGAAGTAGGATCAACGGG70.33670033670033667No Hit
CCTGAGCCTGAGCCGGAGATCCAACTTAA70.33670033670033667No Hit
TCCTGAGCCTGAGCCGGAGATCCAACTTA60.2886002886002886No Hit
TTAGCGCGCCCGTACCCAGCAACTTTCATG60.2886002886002886No Hit
TTAGAGAGTAGATGAGGGACCTCCTGTT60.2886002886002886No Hit
TCAGTAAGGGGTACAGGGACGTCAGGGGA60.2886002886002886No Hit
TTCTCAGTAAGGGGTACAGGGACGTCAGGG60.2886002886002886No Hit
TCACGAGGAGGTAAAAATGGAAGGATAGGA60.2886002886002886No Hit
AACCAGTTGATAAGGCTCGTCGTGCCAAA60.2886002886002886No Hit
TGGGAAGGCAATCGGAGAAGGAGGACATC60.2886002886002886No Hit
TGTACCCGGGTACTGCACAACACTGTCCAAC60.2886002886002886No Hit
GACACAATGTCGTCGAGCACATCATAAAGG60.2886002886002886No Hit
TAGGGGAGTAGCTCCGTGGTCTGGTGAG50.24050024050024052No Hit
AGCCAAGCCTGAGATCGAACACATTGAGAA50.24050024050024052No Hit
TGAGGGACTCACGCTCAGCACGAGAA50.24050024050024052No Hit
TGAGGGACTCACGCTCAGCACGAGAAAG50.24050024050024052No Hit
TAGGTGGGAGCTAACTGCAACTTGAGCGT50.24050024050024052No Hit
TATCCATTATTCTGACCCCCGAGGTAGAA50.24050024050024052No Hit
TGAGAGCTGGTGTCAAAATAAATTCTGTC50.24050024050024052No Hit
CGCTTAATCCACGCGCCATGCTATCGCCT50.24050024050024052No Hit
TAGGCGGTCGGAACAAGGGTCAAAGTTGG50.24050024050024052No Hit
TGAAAAGAACTTACTCCCATGGTACGAACC50.24050024050024052No Hit
TGAAGGTGGAATGAAGACTGGGAAGAGGC50.24050024050024052No Hit
TGCAAACGGCGCGGATCAGATCAACTC50.24050024050024052No Hit
TCGGAACAAGGGTCAAAGTTGGCGTGG50.24050024050024052No Hit
TAGAATAGGAACCGGTGGAGATGTAGCT50.24050024050024052No Hit
TTAGCGCGCCCGTACCCAGCAACTTTCATGAT50.24050024050024052No Hit
TCCACCCAAGCCAAGCCTGAGATCGAACACA50.24050024050024052No Hit
TATGTCGTCCTGAGCCTGAGCCGGAGAT50.24050024050024052No Hit
TGAACAAGGTCCAATGAGGAGACAGCTCT50.24050024050024052No Hit
TACGTCCATTATCCCACCAAGTGCCATAGAC50.24050024050024052No Hit
TGTACAGTACAAGGAAAAGTTGTTGAGAGC50.24050024050024052No Hit
TTAGGGCTTCCAATGAAGGACATCCACT40.1924001924001924No Hit
TTCTCAAGATGAGAACCTAGGCGGTCGGAAC40.1924001924001924No Hit
TCCAAAAGGTTAGGGCTTCCAATGAAGGAC40.1924001924001924No Hit
CTTATGTCGTCCTGAGCCTGAGCCGGAG40.1924001924001924No Hit
TCCATACCAAGCTTATGTCGTCCTGAGCCT40.1924001924001924No Hit
TGCAAACGGCGCGGATCAGATCAACTCCGTC40.1924001924001924No Hit
TAGGGGAGTAGCTCCGTGGTCTGGTGAGG40.1924001924001924No Hit
TCCACTTAAACCAGTTGATAAGGCTCGTCG40.1924001924001924No Hit
CGGAAGTTGTCCATCTTAGCGTGCTGGT40.1924001924001924No Hit
TACAGAGTCGATTTGAAGCAGTTTGCCCAC40.1924001924001924No Hit
TGGGAAGCGGAAGGACAAATATGGTACGAG40.1924001924001924No Hit
TGCATCTGTGAACCCAGGATTCGGGGG40.1924001924001924No Hit
TCGGAAGTTGTCCATCTTAGCGTGCTGG40.1924001924001924No Hit
TGAGGGACTCACGCTCAGCACGAGAAAGAA40.1924001924001924No Hit
TAAACCAGTTGATAAGGCTCGTCGTGCCAA40.1924001924001924No Hit
TACCCGGGTACTGCACAACACTGTCCAACA40.1924001924001924No Hit
TAGGAACCGGTGGAGATGTAGCTAGCGTC40.1924001924001924No Hit
TATTCTGCAGACGTCGGCCCATGTGGCGGC40.1924001924001924No Hit
TACAGAGTCGATTTGAAGCAGTTTGCCCACT40.1924001924001924No Hit
AAACCAGTTGATAAGGCTCGTCGTGCCAAA40.1924001924001924No Hit
TTACAGAGTCGATTTGAAGCAGTTTGCCCAC40.1924001924001924No Hit
TATGTTATCAAGGCGTGTTGAACATGTC40.1924001924001924No Hit
TCAACTTCTCGAGATCGTAGGCAAAGTCCC40.1924001924001924No Hit
TTATCCATTATTCTGACCCCCGAGGTAGAAT40.1924001924001924No Hit
TTATCCATTATTCTGACCCCCGAGGTAGAAA40.1924001924001924No Hit
TAGACTCTCGCTTAATCCACGCGCCATGC40.1924001924001924No Hit
TTTGCCCACTGTGCTTATGTTATCAAGGCGTG40.1924001924001924No Hit
TTAATCCACGCGCCATGCTATCGCCTACG40.1924001924001924No Hit
TCCAGGTCTCATCGGCAAGACAACCAGC40.1924001924001924No Hit
TCCCCGGATATCTTTGACGAAAGACT30.1443001443001443No Hit
TGCATCTGGGAACCCAGGATTCGGGGG30.1443001443001443No Hit
TCTCAGTAAGGGGTACAGGGACGTCAGG30.1443001443001443No Hit
TGTGGCGGCGCTAGCACTAGTGCAAACGGC30.1443001443001443No Hit
TCTGACCCCCGAGGTAGAATAGGAACCGGTT30.1443001443001443No Hit
TCGGAACATAGAACGAGGAGAGAAGACTGC30.1443001443001443No Hit
TGTCGTCCTGAGCCTGAGCCGGAGATCCAC30.1443001443001443No Hit
TGACGAAAGACTTGGGAGAGCACGACACA30.1443001443001443No Hit
TATGGGAAGGCAATCGGAGAAGGAGGA30.1443001443001443No Hit
TAAGGGGTACAGGGACGTCAGGGGAGA30.1443001443001443No Hit
AAAGTTGGCGTGGCGATGGGTCCAGGT30.1443001443001443No Hit
CTCTCGCTTAATCCACGCGCCATGCTATC30.1443001443001443No Hit
TCCCACCAAGTGCCATAGACGAAACCCTCT30.1443001443001443No Hit
TAGTCGAGCGCCACATGCCGATGCATCTG30.1443001443001443No Hit
CCTGAGCCTGAGCCGGAGATCCAACTT30.1443001443001443No Hit
TCTGATGTCGTCCGAAGATTGCAATGAAGC30.1443001443001443No Hit
TTGAGCGTGAATGGTGGGAAGCGGAAGGAT30.1443001443001443No Hit
TCACTTTTCTCACACAAGAGTCCAGCTCACAC30.1443001443001443No Hit
TTAAACCAGTTGATAAGGCTCGTCGTGC30.1443001443001443No Hit
TCCTGAGCCTGAGCCGGAGATCCAACTT30.1443001443001443No Hit
TCGGAGAACGAGATACCGTCAGGCCCATATC30.1443001443001443No Hit
TATTCTGCAGACGTCGGCCCATGTGGCGG30.1443001443001443No Hit
CCTGAGCCTGAGCCGGAGATCCAACTTAAA30.1443001443001443No Hit
TGAAGGTGGAATGAAGACTGGGAAGAGG30.1443001443001443No Hit
CCGGGTACTGCACAACACTGTCCAACACC30.1443001443001443No Hit
TCAGATCAACTCCGTCGACGGAAAGGAT30.1443001443001443No Hit
CGCGTATTCTGCAGACGTCGGCCCATG30.1443001443001443No Hit
TTCTCACACAAGAGTCCAGCTCACACAAGAG30.1443001443001443No Hit
TGGTATGAGAAACTTTATGCATGGCGCGCAT30.1443001443001443No Hit
TCCCACCAAGTGCCATAGACGAAACCCT30.1443001443001443No Hit
AACCAGTTGATAAGGCTCGTCGTGCCAAAC30.1443001443001443No Hit
TGAGACCTGGACGGTCACGGCCTTGACACC30.1443001443001443No Hit
TAAACCAGTTGATAAGGCTCGTCGTGCC30.1443001443001443No Hit
TCGGAACAAGGGTCAAAGTTGGCGTGGCGC30.1443001443001443No Hit
ACTCCGTCGACGGAAACGATTTCCCCTTA30.1443001443001443No Hit
TCGGAACAAGGGTCAAAGTTGGCGTGGCGAT30.1443001443001443No Hit
TCAACGGGTATGGGTTCAAGGAATTGAGT30.1443001443001443No Hit
TCCACGCGCCATGCTATCGCCTACGATTAAC30.1443001443001443No Hit
TTTGCCCACTGTGCTCATGTTATCAAGGCGTG30.1443001443001443No Hit
TGCATCTGGGAACCCAGGATTCGGGGGTG30.1443001443001443No Hit
TAACTGAGACCTGGACGGTCACGGCCTTGAC30.1443001443001443No Hit
TACAGAGTCGATTTGAAGCAGTTTTCCCAC30.1443001443001443No Hit
TCGGAACATAGAACGAGGAGAGAAGACT30.1443001443001443No Hit
TAGCACATAGACTCTCGCTTAATCCACGC30.1443001443001443No Hit
TTCTCCACCCAAGCCAAGCCGGAGATCGAAC30.1443001443001443No Hit
TCCGTCGACGGAAACGATTTCCCCTTAGC30.1443001443001443No Hit
TCACGAGGAGGTAAAAATGGAAGGATAG30.1443001443001443No Hit
TGATGTCGTCCGAAGATTGCAATGAAGC30.1443001443001443No Hit
TCGTCCTGAGCCTGAGCCGGAGATCCAACT30.1443001443001443No Hit
TTAAACCAGTTGATAAGGCTCGTCGTGCCAAA30.1443001443001443No Hit
TCCATCTTAGCGTGCTGGTCGAAAGAGCT30.1443001443001443No Hit
TCACGAAGTCCCTCTCTGATGTCGTCCG30.1443001443001443No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)