FastQCFastQC Report
Wed 31 Mar 2021
S33.15-35nt.orbivirus.NODE_9699.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.orbivirus.NODE_9699.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1291
Sequences flagged as poor quality0
Sequence length17-35
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGGACACCGCTCAAGGATGAAAGATCATC15712.161115414407437No Hit
TTAGAGAAGAGACACCCTTCAGGTACTGT856.584043377226956No Hit
TACACCAACCAAGTCGCCAAACTCGATAGCCCT634.8799380325329205No Hit
TCAATAGATCCACTTCCAGACCTCGCATC503.8729666924864445No Hit
TAAGCACATAACCAAAGATTGGAGGGATG362.78853601859024No Hit
TAGCCCTTACAGAGACCTCACACGGAAGAG362.78853601859024No Hit
TGAACAAGGCCCAAACGCAAAGCATAAGTG312.4012393493415956No Hit
TCCTTGGGTATGAACAATCCGAATCGGGC302.3237800154918666No Hit
TCCACTTAGAATCCTCTCTAGACCGCACCT241.8590240123934934No Hit
TTCTTGTTCAGAATCTTAACTATGGCGCC221.7041053446940357No Hit
TTCAATAGATCCACTTCCAGACCTCGCATC221.7041053446940357No Hit
TGCTTTGGCGGAGGACCCTTGAGTACCT171.3168086754453912No Hit
TAAGCTGTCGCAGACGTTCAATAGATCCAC161.2393493415956625No Hit
TAACTCCTGAGAGAACTCAAGAAAGTGGG141.0844306738962044No Hit
TGAACAATCCGAATCGGGCGTAGGTCTGGAC131.0069713400464757No Hit
TAAGCCAAATCTCTCGAACGATCTCACGAT120.9295120061967467No Hit
TCCACTTAGAATCCTCTCTAGACCGCACC120.9295120061967467No Hit
TCTTGTTCAGAATCTTAACTATGGCGCCTC100.774593338497289No Hit
TAACGGCGGAATCGATGGTGACTCTCCTGC100.774593338497289No Hit
TCTTGTTCAGAATCTTAACTATGGCGCCT90.69713400464756No Hit
TTCTTGTTCAGAATCTTAACTATGGCGCCT90.69713400464756No Hit
TTGGCGGAGGACCCTTGAGTACCTTGGGGA90.69713400464756No Hit
TGTTACACCAACCAAGTCGCCAAACTCGAT90.69713400464756No Hit
TCTGATCCTTGGGTATGAACAATCCGAAT90.69713400464756No Hit
TACGGGATGAAACATAACCAATCGGGTCGC80.6196746707978312No Hit
TCAACGTCGTCTCCAACTACGCATAGGGA80.6196746707978312No Hit
TGAGACACCCTACGGATAGGACACCGCTC80.6196746707978312No Hit
TCAGAAAGACCAACTCCAACACCAGTGTT80.6196746707978312No Hit
AAGGACGATTTCTCTCCACCCAGGAGAT70.5422153369481022No Hit
TATGAACAATCCGAATCGGGCGTAGGTC70.5422153369481022No Hit
TCCACTTAGAATCCTCTCTAGACCGCACCTC60.46475600309837334No Hit
TCCTGAGAGAACTCAAGAAAGTGGGAGACT60.46475600309837334No Hit
TGAAACATAACCAATCGGGTCGCGAATCGT60.46475600309837334No Hit
TCTTAACTATGGCGCCTCTAATCGAAAAAC60.46475600309837334No Hit
TCTTGTTCAGAATCTTAACTATGGCGCC50.3872966692486445No Hit
TCTGGACATATTCAGCTGAAAGAGGGCATA50.3872966692486445No Hit
TGAGCGAAAGGACGATTTCTCTCCACCCAG50.3872966692486445No Hit
TAGGACACCGCTCAAGGATGAAAGATCA50.3872966692486445No Hit
TATGAACAATCCGAATCGGGCGTAGGTCT50.3872966692486445No Hit
ACCAACCAAGTCGCCAAACTCGATAGCCCT50.3872966692486445No Hit
TAGATCCACTTCCAGACCTCGCATCATTC40.3098373353989156No Hit
TCTCAACGTCGTCTCCAACTACGCATAGG40.3098373353989156No Hit
TAAAAACGCGATCCACTTAGAATCCTCTCT40.3098373353989156No Hit
TTGGGTATGAACAATCCGAATCGGGCGT40.3098373353989156No Hit
ATCCACTTAGAATCCTCTCTAGACCGCACC40.3098373353989156No Hit
TAGCGATATTCATGATCTGATCCTTGGGT40.3098373353989156No Hit
TAGAGAACCTGTAAGCACATAACCAAAGAT40.3098373353989156No Hit
TGGGCTCCGAATTTTCTCTCTTCTGAAATT40.3098373353989156No Hit
TGGGTATGAACAATCCGAATCGGGCGTAGG40.3098373353989156No Hit
TTAACTCCTGAGAGAACTCAAGAAAGTGGG40.3098373353989156No Hit
TTAGAATCCTCTCTAGACCGCACCTCCTT40.3098373353989156No Hit
TTAACTCCTGAGAGAACTCAAGAAAGTGG40.3098373353989156No Hit
TTAGAGAAGAGACACCCTTCAGGTACTG40.3098373353989156No Hit
TACAAAATCCGAGGCGTAGGTGCGAGAGG40.3098373353989156No Hit
TAGGTCTGGACATATTCAGCTGAAAGAGG40.3098373353989156No Hit
TGAAAGAAGAGCGCGTCCGTAATAACAAT40.3098373353989156No Hit
TCCGAGGCGTAGGTGCGAGAGGTACTTCT40.3098373353989156No Hit
TCCCCCCCAGTAATTCGGAGTTCCCTCAGCT40.3098373353989156No Hit
TCCGAGGCGTAGGTGCGAGAGGTACTTC40.3098373353989156No Hit
TACACCAACCAAGTCGCCAAACTCGATAGCCC30.23237800154918667No Hit
TAACTCACCAGAAGCCTCGCATTCAAGAT30.23237800154918667No Hit
TGGTGCCACGTGTAAATTTTGTACAATAAA30.23237800154918667No Hit
TTCTAACGGCGGAATCGATGGTGACTCTCC30.23237800154918667No Hit
TGTGAGTTGGATCGGGTTGGCACCACCG30.23237800154918667No Hit
TGAAAGATCATCTGAGAGCGAACCATGCGT30.23237800154918667No Hit
TTGGCGGAGGACCCTTGAGTACCTTGGG30.23237800154918667No Hit
AGAGTGAGACCTAGACGGGAACCGGGA30.23237800154918667No Hit
TTCAATAGATCCACTTCCAGACCTCGCATCA30.23237800154918667No Hit
TCATTATACTTGTCGGGGCCATGTGAGT30.23237800154918667No Hit
TAGGACACCGCTCAAGGATGAAAGATCATCAG30.23237800154918667No Hit
TCGATGGTGACTCTCCTGCTCTGATCAAAG30.23237800154918667No Hit
TTGTTCAGAATCTTAACTATGGCGCCTCT30.23237800154918667No Hit
TACTCCCCCCCAGTAATTCGGAGTTCCCTCAGC30.23237800154918667No Hit
TAGGGGAAGGTTGCTTTGGCGGAGGACCC30.23237800154918667No Hit
ACGGAAGAGTGAGACCTAGACGGGAACCGG30.23237800154918667No Hit
TAGGACACCGCTCAAGGATGAAAGATCATCTG30.23237800154918667No Hit
TAACCACGGGACATCTTCGAGAGCATGAGCC30.23237800154918667No Hit
TAGCCCTTACAGAGACCTCACACGGAAGA30.23237800154918667No Hit
TAGGACACCGCTCAAGGATGAAAGATCATCT30.23237800154918667No Hit
AACCACGGGACATCTTCGAGAGCATGAGCC30.23237800154918667No Hit
TAGAATCCTCTCTAGACCGCACCTCCTT30.23237800154918667No Hit
TCTCTAGACCGCACCTCCTTGATAGCGAAT30.23237800154918667No Hit
TGTAAGACGGTAGCCTCGGAATGGAGGTC30.23237800154918667No Hit
TAGTAGCTCCTCCTGTGCGCGACCTAGGG30.23237800154918667No Hit
TGGGCTCCGAATTCTCTCTCTTCTGAAATT30.23237800154918667No Hit
TCGGTGAAGGCGAGGGAGGGCTTAGTTT30.23237800154918667No Hit
TAAGGTGGAAAGTAGCGACGTCGATATC30.23237800154918667No Hit
CTTCGAGAGCATGAGCCTCCTACGGGAT20.1549186676994578No Hit
ATGGGCTCCGAATTTTC20.1549186676994578No Hit
TCACGATCTCTTCGGGAGTAATATGGCCC20.1549186676994578No Hit
GTGAGACCTAGACGGGAACCGGGATAAGA20.1549186676994578No Hit
TAACCAATCGGGTCGCGAATCGTGCGAGGC20.1549186676994578No Hit
ACACCAACCAAGTCGCCAAACTCGATAGCCCT20.1549186676994578No Hit
TACAAAATCCGAGGCGTAGGTGCGAGAG20.1549186676994578No Hit
AGATAGAGAACCTGTAAGCACATAACCAAAG20.1549186676994578No Hit
TCAAGAAAGTGGGAGACTATAGAGGAAG20.1549186676994578No Hit
GTACCTTGGGGAAAAGAACGTCTTATACTT20.1549186676994578No Hit
TAGGACACCGCTCAAGGATGAAAGATCATCAA20.1549186676994578No Hit
TTCTTGAGACACCCTACGGATAGGACACC20.1549186676994578No Hit
TCCATACAAAATCCGAGGCGTAGGTGCG20.1549186676994578No Hit
TCAGGTGGAATGGGCATGTTCTTGAG20.1549186676994578No Hit
TACATCAACATCAGAAAGACCAACTCCAACACC20.1549186676994578No Hit
TATATCGGTGAAGGCGAGGGAGGGCTTT20.1549186676994578No Hit
TGAGACCTAGACGGGAACCGGGATAAGAA20.1549186676994578No Hit
ACGGGACATCTTCGAGAGCATGAGCCTCCT20.1549186676994578No Hit
TCTCACTAGCGATATTCATGATCTGATCCT20.1549186676994578No Hit
TAAGCGATGAGTCGACGGAGCAGAGGT20.1549186676994578No Hit
TTATACTTGTCGGGGCCATGTGAGTTGG20.1549186676994578No Hit
TAGGAGGAAATATATCGGTGAAGGCGAGG20.1549186676994578No Hit
TCAAGCTCCGTGCTGTCGAAGCAATACGCCC20.1549186676994578No Hit
TCTTCGAGAGCATGAGCCTCCTACGGGATG20.1549186676994578No Hit
TCAGCGGCGAGAGGTCCCATTATATCAGG20.1549186676994578No Hit
TCAAGCTCCGTGCTGTCGAAGCAATACGCCT20.1549186676994578No Hit
TGAACAATCCGAATCGGGCGTAGGTCTGGA20.1549186676994578No Hit
TGTTCAGAATCTTAACTATGGCGCCTCT20.1549186676994578No Hit
TAAAAATAGCAGTTCATTATACTTGTCGGGGCCA20.1549186676994578No Hit
TCCGTTTTACTTTGGAGTTGCGTAACCGAG20.1549186676994578No Hit
TCCTCTCTAGACCGCACCTCCTTGATAGCGAAA20.1549186676994578No Hit
TTGGCGGAGGACCCTTGAGTACCTTGGGGAT20.1549186676994578No Hit
TGGTGACTCTCCTGCTCTGATCAAAGG20.1549186676994578No Hit
TTGGCGGAGGACCCTTGAGTACCTTGGGG20.1549186676994578No Hit
AGAGACACCCTTCAGGTACTGTAAGACGG20.1549186676994578No Hit
TGCATGAGAGCGGTGTGCGTAGCATTGTG20.1549186676994578No Hit
TAGCCTCGGAATGGAGTTCGTGGGAGG20.1549186676994578No Hit
TGTAAGACGGTAGCCTCGGAA20.1549186676994578No Hit
AAGCTGTCGCAGACGTTCAATAGATCCAC20.1549186676994578No Hit
TTCGAGAGCATGAGCCTCCTACGGGATGAA20.1549186676994578No Hit
AAGGACGATTTCTCTCCACCCAGGA20.1549186676994578No Hit
TAACGGCGGAATCGATGGTGACTCTCC20.1549186676994578No Hit
TTCAGCTGAAAGAGGGCATAGAAACGTCT20.1549186676994578No Hit
CAATTACACCAGCGCTCTTTACGACTTAAGC20.1549186676994578No Hit
TTAGAGAAGAGACACCCTTCAGGTACTGTT20.1549186676994578No Hit
TACCTTGGGGAAAAGAACGTCTTATACTT20.1549186676994578No Hit
TATTCAGCTGAAAGAGGGCATAGAAACGTC20.1549186676994578No Hit
TCAACACGGGATCTCTACTGATAAGCTGTC20.1549186676994578No Hit
TCAATAGATCCACTTCCAGACCTCGCATCA20.1549186676994578No Hit
TCGGAATGGAGTTCGTGGGAGGAGATAG20.1549186676994578No Hit
TTAAGCGATGAGTCGACGGAGCAGAGGTC20.1549186676994578No Hit
TTACACCAACCAAGTCGCCAAACTCGATAGCCCT20.1549186676994578No Hit
TGGGTATGAACAATCCGAATCGGGCGTAGGT20.1549186676994578No Hit
TAAGAACAAAGTGGTTAACTCACCAGAAGCCC20.1549186676994578No Hit
TCCATACAAAATCCGAGGCGTAGGTGCGAGA20.1549186676994578No Hit
ACGGGATGAAACATAACCAATCGGGTCGCG20.1549186676994578No Hit
AGCATAAGTGAGCGAAAGGACGATTTCTCT20.1549186676994578No Hit
TAGGACACCGCTCAAGGATGAAAGATCAT20.1549186676994578No Hit
TCCATACAAAATCCGAGGCGTAGGTGCGA20.1549186676994578No Hit
TTCAGCGGCGAGAGGTCCCATTATATCAGG20.1549186676994578No Hit
TTTGGCGGAGGACCCTTGAGTACCTTGGGG20.1549186676994578No Hit
TAGCGACGTCGATATCTGTTCTAACGGC20.1549186676994578No Hit
TGACAGAATCACAAAAACCCATGCGCTTA20.1549186676994578No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)